Rh1CG210500

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000382
Physical Location & Seq
Forward (+)
45394273 .. 45409625
15353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG210500.1

Sequence Viewer

Length: 804 bp
ATGGAAGAGGAAAAGGGTCCAGTTTGTGTAACGGGTGGAACTGGATTCATCGGTTCATGGCTGGTTATGAAGCTTCTGCAAAATGGTTACACTGTTCGAACTACTGTTAGACCTGATCCAAAATGCAAGAGAGACATCAACTACCTCACAAGCCTGCCTAGAGCATCGGAGAAGCTTCACATCTTCAATGGGGATCTGAACCAACCTGAAAGTTTTAATGTAGCAATTGAAGGATGCACCGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAATTTGATGAAGCATCGGTAACCAAACAATCTGTAGAAGGTACCCTAGGCATATTGAAAGCTTGCCTAAATGCCAAGACTGTGAAAAAGGTTGTTTACACTTCTAGTGCAGCAACTGTTGCTTATAGTGGCAACAACAAAGACATGGCGGATGAGAGTTCATGGAGTGATATAGAGTACCATAGGTCTCTTGGATTATTTAGGAGTTCATCGTATGTGGCTGCTAAGACCAAAACAGAGCAAGCAGTACTGGAATTTGCAGAAAAAAATGGATTAGAACTTGTGACTTTGATCCCTCCATTCGTCCTGGTGGTTTCATCTGCAAAGATCTCCCTGAAAATGAATGATCCTGACGATTTGCTCGAGAATGCGAATGTTGTTGCTCCCGTGACCTCCGTCGCTGGCCTTACCGTCGCCGAGAGCCTCGACGAGCAGCGCCACAAACCTGTTGCATTTGGAGAGAAACTTCCTGTTCTCTTCCGACTCCTTGGCGAACCGCGCAATCTTTGCCAGCAAATCCACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

267

Amino Acids

29.22

Weight (kDa)

6.33

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 135 6.2e-09 NmrA-like family
Epimerase PF01370 8 - 192 1.5e-19 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 137 1.8e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 188 1.9e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 190 4.5e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 198 1.4e-13 Male sterility protein
NAD_binding_10 PF13460 12 - 132 2.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 1 cut(s) 775
AciI CCGC 2 cut(s) 425, 773
AclWI GGATC 4 cut(s) 110, 201, 562, 617
AcsI RAATTY 2 cut(s) 278, 530
AfaI GTAC 3 cut(s) 319, 455, 525
AgsI TTSAA 3 cut(s) 187, 230, 334
AjnI CCWGG 1 cut(s) 582
AluBI AGCT 3 cut(s) 73, 175, 338
AluI AGCT 3 cut(s) 73, 175, 338
Alw26I GTCTC 2 cut(s) 126, 468
AlwI GGATC 4 cut(s) 110, 201, 562, 617
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 3 cut(s) 386, 497, 709
ApoI RAATTY 2 cut(s) 278, 530
Asp718I GGTACC 1 cut(s) 317
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 2 cut(s) 714, 777
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 322
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 3 cut(s) 398, 484, 721
BciT130I CCWGG 1 cut(s) 584
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 126, 468
BfaI CTAG 3 cut(s) 159, 323, 381
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 715
BglII AGATCT 1 cut(s) 603
BisI GCNGC 3 cut(s) 387, 498, 710
BlnI CCTAGG 1 cut(s) 322
BlsI GCNGC 3 cut(s) 388, 499, 711
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 241, 584
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 319
BmrFI CCNGG 2 cut(s) 241, 584
BmsI GCATC 3 cut(s) 173, 224, 299
BoxI GACNNNNGTC 1 cut(s) 671
BpiI GAAGAC 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 3 cut(s) 240, 322, 763
Bse1I ACTGG 3 cut(s) 20, 46, 531
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 3 cut(s) 240, 322, 763
BseGI GGATG 3 cut(s) 239, 259, 433
BseNI ACTGG 3 cut(s) 20, 46, 531
BseXI GCAGC 3 cut(s) 398, 484, 721
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 775
BshFI GGCC 1 cut(s) 681
BshNI GGYRCC 1 cut(s) 317
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 1 cut(s) 240
BsmAI GTCTC 2 cut(s) 126, 468
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 1 cut(s) 681
Bso31I GGTCTC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 638
Bsp119I TTCGAA 1 cut(s) 97
Bsp143I GATC 5 cut(s) 115, 193, 567, 603, 622
BspACI CCGC 2 cut(s) 425, 773
BspANI GGCC 1 cut(s) 681
BspFNI CGCG 1 cut(s) 775
BspLI GGNNCC 2 cut(s) 18, 319
BspPI GGATC 4 cut(s) 110, 201, 562, 617
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 468
BsrI ACTGG 3 cut(s) 20, 46, 531
BssECI CCNNGG 3 cut(s) 240, 322, 763
BssMI GATC 5 cut(s) 115, 193, 567, 603, 622
BssT1I CCWWGG 2 cut(s) 322, 763
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 5 cut(s) 94, 106, 358, 394, 688
Bst6I CTCTTC 1 cut(s) 758
BstAPI GCANNNNNTGC 2 cut(s) 395, 783
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 5 cut(s) 155, 340, 519, 679, 788
BstDEI CTNAG 1 cut(s) 501
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 3 cut(s) 239, 259, 433
BstFNI CGCG 1 cut(s) 775
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 2 cut(s) 714, 777
BstKTI GATC 5 cut(s) 118, 196, 570, 606, 625
BstMAI GTCTC 2 cut(s) 126, 468
BstMBI GATC 5 cut(s) 115, 193, 567, 603, 622
BstMWI GCNNNNNNNGC 3 cut(s) 395, 774, 783
BstNI CCWGG 1 cut(s) 584
BstPAI GACNNNNGTC 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 239, 582
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 775
BstV1I GCAGC 3 cut(s) 398, 484, 721
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 193, 603
BstYI RGATCY 2 cut(s) 193, 603
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 3 cut(s) 239, 259, 433
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 5 cut(s) 155, 340, 519, 679, 788
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 2 cut(s) 714, 777
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 3 cut(s) 318, 454, 524
CviAII CATG 4 cut(s) 57, 251, 421, 438
CviJI RGCY 8 cut(s) 61, 73, 153, 175, 338, 497, 681, 699
CviKI_1 RGCY 8 cut(s) 61, 73, 153, 175, 338, 497, 681, 699
CviQI GTAC 3 cut(s) 318, 454, 524
DdeI CTNAG 1 cut(s) 501
DpnI GATC 5 cut(s) 117, 195, 569, 605, 624
DpnII GATC 5 cut(s) 115, 193, 567, 603, 622
Eam1104I CTCTTC 1 cut(s) 758
EarI CTCTTC 1 cut(s) 758
EciI GGCGGA 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 322, 763
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 17
Eco88I CYCGRG 1 cut(s) 638
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 582
EcoT14I CCWWGG 2 cut(s) 322, 763
ErhI CCWWGG 2 cut(s) 322, 763
FaeI CATG 4 cut(s) 60, 254, 424, 441
FatI CATG 4 cut(s) 56, 250, 420, 437
Fnu4HI GCNGC 3 cut(s) 387, 498, 710
FokI GGATG 3 cut(s) 246, 246, 440
Fsp4HI GCNGC 3 cut(s) 387, 498, 710
FspBI CTAG 3 cut(s) 159, 323, 381
GlaI GCGC 2 cut(s) 713, 776
GluI GCNGC 3 cut(s) 387, 498, 710
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 1 cut(s) 681
HapII CCGG 1 cut(s) 240
HhaI GCGC 2 cut(s) 714, 777
Hin1II CATG 4 cut(s) 60, 254, 424, 441
Hin6I GCGC 2 cut(s) 712, 775
HinP1I GCGC 2 cut(s) 712, 775
HindIII AAGCTT 3 cut(s) 71, 173, 336
HinfI GANTC 2 cut(s) 45, 759
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 3 cut(s) 169, 198, 758
Hpy188III TCNNGA 2 cut(s) 626, 640
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 3 cut(s) 677, 692, 707
HpyAV CCTTC 2 cut(s) 224, 308
HpyCH4III ACNGT 5 cut(s) 94, 106, 358, 394, 688
HpyCH4V TGCA 7 cut(s) 79, 126, 237, 386, 536, 599, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 774, 783
HpyF3I CTNAG 1 cut(s) 501
Hsp92II CATG 4 cut(s) 60, 254, 424, 441
HspAI GCGC 2 cut(s) 712, 775
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 5 cut(s) 115, 193, 567, 603, 622
LmnI GCTCC 1 cut(s) 664
Lsp1109I GCAGC 3 cut(s) 398, 484, 721
LweI GCATC 3 cut(s) 173, 224, 299
MaeI CTAG 3 cut(s) 159, 323, 381
MaeIII GTNAC 5 cut(s) 28, 86, 295, 559, 664
MalI GATC 5 cut(s) 117, 195, 569, 605, 624
MboI GATC 5 cut(s) 115, 193, 567, 603, 622
MboII GAAGA 4 cut(s) 17, 175, 238, 745
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 2 cut(s) 193, 603
MluCI AATT 3 cut(s) 225, 278, 530
MlyI GAGTC 1 cut(s) 753
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 4 cut(s) 155, 582, 679, 710
MseI TTAA 1 cut(s) 216
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 241, 584
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 1 cut(s) 584
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 3 cut(s) 395, 774, 783
NciI CCSGG 1 cut(s) 241
NdeII GATC 5 cut(s) 115, 193, 567, 603, 622
NlaIII CATG 4 cut(s) 60, 254, 424, 441
NlaIV GGNNCC 2 cut(s) 18, 319
NmeAIII GCCGAG 1 cut(s) 718
NmuCI GTSAC 2 cut(s) 559, 664
NspV TTCGAA 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PctI GAATGC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 45
PkrI GCNGC 3 cut(s) 388, 499, 711
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
PshAI GACNNNNGTC 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 582
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 2 cut(s) 18, 319
PspPI GGNCC 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 193, 603
RsaI GTAC 3 cut(s) 319, 455, 525
RsaNI GTAC 3 cut(s) 318, 454, 524
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 3 cut(s) 387, 498, 710
Sau3AI GATC 5 cut(s) 115, 193, 567, 603, 622
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 2 cut(s) 241, 584
SfaNI GCATC 3 cut(s) 173, 224, 299
SfcI CTRYAG 1 cut(s) 309
Sfr274I CTCGAG 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 3 cut(s) 225, 278, 530
SsiI CCGC 2 cut(s) 425, 773
SspMI CTAG 3 cut(s) 159, 323, 381
StyD4I CCNGG 2 cut(s) 239, 582
StyI CCWWGG 2 cut(s) 322, 763
TaaI ACNGT 5 cut(s) 94, 106, 358, 394, 688
TaqI TCGA 3 cut(s) 97, 639, 702
TasI AATT 3 cut(s) 225, 278, 530
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 2 cut(s) 559, 664
TseI GCWGC 3 cut(s) 386, 497, 709
Tsp45I GTSAC 2 cut(s) 559, 664
TspDTI ATGAA 8 cut(s) 37, 45, 83, 300, 426, 474, 582, 632
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 278, 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XhoI CTCGAG 1 cut(s) 638
XmaJI CCTAGG 1 cut(s) 322
XspI CTAG 3 cut(s) 159, 323, 381
ZrmI AGTACT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.