FvH4_7g11780

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000382
Physical Location & Seq
Forward (+)
10797095 .. 10799367
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11780.t1

Sequence Viewer

Length: 1005 bp
ATGGAAGAGGAAAAAGGTCCAGTTTGCGTGACGGGTGGAACTGGATTCATCGGCTCATGGCTCGTTATGAAGCTTCTGCAAAATGGTTACACTGTTCGAACTACCGTTAGACCTGACCCAGAATGCAAGAGAGACATCAGCTACCTCACAAGCCTGCCTAGAGCATCAGAGAAGCTTCACATCTTTGATGGGGATCTGAACCAACCTGAAAGTTTCAATGCAGCAATCGAAGGATGCACCGGGGTCTTCCATGTAGCTCATCCTATGTCTAACAAAGAACTTGATGAAGCATCGGTAACCAAACAATCTGTAGAAGGAACCCTAGGAATATTGAAAGCTTGCTTAAATGCCAAGACTGTGAAGAAGGTTGTTTACACTTCTAGTGCAGCAACTGTTGCATATAGTGGCAGCAAAAAAGACATAGTGGATGAGAGTTCATGGAGTGACATTGAATATCATAGGTCCCTTGGAATATTTAGGAGTTCATCATATGTGGCTGCTAAGACCAAAACAGAGCAAGCGGTACTGCAATTTGCGGACCAAAATGGATTGGAACTTGTTACTCTAATCCCTCCATTCGTCCTTGGTGGATTCATCTGCAAAGATCTTCCTGGTTCAGTAGAGTTGATCATATCTATGATGTTCGGAAAAGTAGACAACTTCACACAGCTTCGCAAGTTAAGTTTGATACATGTAGATGATTTGGCTAGAGCCCATATATTTCTTTTCGAACATTCTAATGCCAAGGGGAGGTACATTTGCTCTTCAACTCCAACATCAATGGACGAAATGCACCAATTTTTTTCTGCAAAATACCCTGAATTTCACATACCCCCAATTCAATCACTGAAAGAAACTTACAAAATTTGTGGTTTCTCATCGATGAAGCTCCTGAATACTGGATTCGAGTTTAAGCATGGTCTCGATGACATATTGGATGGCGCAATTCAATCTTGCAGAGAAAAGGGTGTTCTAGAAAAGCTCTTTGAAAAAATTGACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

335

Amino Acids

36.98

Weight (kDa)

6.08

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 137 2.5e-09 NmrA-like family
Epimerase PF01370 8 - 249 1.4e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 241 2.6e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 219 2.7e-14 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 190 1.4e-13 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 131 6.6e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AciI CCGC 2 cut(s) 521, 536
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 2 cut(s) 821, 864
AfaI GTAC 2 cut(s) 525, 755
AfiI CCNNNNNNNGG 1 cut(s) 750
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 7 cut(s) 217, 334, 452, 768, 842, 950, 989
AjnI CCWGG 1 cut(s) 610
AluBI AGCT 8 cut(s) 73, 141, 175, 257, 338, 670, 889, 982
AluI AGCT 8 cut(s) 73, 141, 175, 257, 338, 670, 889, 982
Alw26I GTCTC 2 cut(s) 126, 926
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 1 cut(s) 392
ApeKI GCWGC 4 cut(s) 221, 386, 408, 497
ApoI RAATTY 2 cut(s) 821, 864
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 1 cut(s) 944
AspS9I GGNCC 3 cut(s) 17, 462, 538
AsuC2I CCSGG 1 cut(s) 241
AsuII TTCGAA 2 cut(s) 97, 729
AvaII GGWCC 3 cut(s) 17, 462, 538
AvrII CCTAGG 1 cut(s) 322
BanII GRGCYC 1 cut(s) 715
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 4 cut(s) 233, 398, 420, 484
BccI CCATC 2 cut(s) 182, 932
BciT130I CCWGG 1 cut(s) 612
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 126, 926
BfaI CTAG 5 cut(s) 159, 323, 381, 708, 974
BfmI CTRYAG 1 cut(s) 309
BglII AGATCT 1 cut(s) 604
BisI GCNGC 4 cut(s) 222, 387, 409, 498
BlnI CCTAGG 1 cut(s) 322
BlsI GCNGC 4 cut(s) 223, 388, 410, 499
Bme1390I CCNGG 2 cut(s) 241, 612
Bme18I GGWCC 3 cut(s) 17, 462, 538
BmgT120I GGNCC 3 cut(s) 17, 462, 538
BmiI GGNNCC 2 cut(s) 319, 464
BmrFI CCNGG 2 cut(s) 241, 612
BmsI GCATC 3 cut(s) 173, 224, 299
BpiI GAAGAC 1 cut(s) 238
Bpu14I TTCGAA 2 cut(s) 97, 729
BpuMI CCSGG 1 cut(s) 241
Bsa29I ATCGAT 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 926
BsaJI CCNNGG 5 cut(s) 240, 322, 466, 583, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 3 cut(s) 20, 46, 904
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 612
BseCI ATCGAT 1 cut(s) 881
BseDI CCNNGG 5 cut(s) 240, 322, 466, 583, 744
BseGI GGATG 4 cut(s) 239, 259, 433, 943
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 750
BseNI ACTGG 3 cut(s) 20, 46, 904
BseXI GCAGC 4 cut(s) 233, 398, 420, 484
BsgI GTGCAG 1 cut(s) 405
BshVI ATCGAT 1 cut(s) 881
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 2 cut(s) 126, 926
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 926
Bsp119I TTCGAA 2 cut(s) 97, 729
Bsp1286I GDGCHC 1 cut(s) 715
Bsp143I GATC 3 cut(s) 193, 604, 627
BspACI CCGC 2 cut(s) 521, 536
BspDI ATCGAT 1 cut(s) 881
BspLI GGNNCC 2 cut(s) 319, 464
BspPI GGATC 1 cut(s) 201
BspQI GCTCTTC 1 cut(s) 769
BspT104I TTCGAA 2 cut(s) 97, 729
BspTNI GGTCTC 1 cut(s) 926
BsrI ACTGG 3 cut(s) 20, 46, 904
BssECI CCNNGG 5 cut(s) 240, 322, 466, 583, 744
BssMI GATC 3 cut(s) 193, 604, 627
BssT1I CCWWGG 4 cut(s) 322, 466, 583, 744
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 4 cut(s) 94, 106, 358, 394
Bst6I CTCTTC 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBI TTCGAA 2 cut(s) 97, 729
BstC8I GCNNGC 3 cut(s) 155, 340, 519
BstDEI CTNAG 1 cut(s) 501
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 4 cut(s) 239, 259, 433, 943
BstHHI GCGC 1 cut(s) 944
BstKTI GATC 3 cut(s) 196, 607, 630
BstMAI GTCTC 2 cut(s) 126, 926
BstMBI GATC 3 cut(s) 193, 604, 627
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 695
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 239, 610
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 4 cut(s) 233, 398, 420, 484
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 193, 604
BstYI RGATCY 2 cut(s) 193, 604
Bsu15I ATCGAT 1 cut(s) 881
BsuTUI ATCGAT 1 cut(s) 881
BtsCI GGATG 4 cut(s) 239, 259, 433, 943
BtsIMutI CAGTG 2 cut(s) 90, 845
Cac8I GCNNGC 3 cut(s) 155, 340, 519
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 944
Cfr13I GGNCC 3 cut(s) 17, 462, 538
ClaI ATCGAT 1 cut(s) 881
Csp6I GTAC 2 cut(s) 524, 754
CspCI CAANNNNNGTGG 2 cut(s) 850, 885
CviAII CATG 6 cut(s) 57, 251, 438, 692, 917, 1002
CviQI GTAC 2 cut(s) 524, 754
DdeI CTNAG 1 cut(s) 501
DpnI GATC 3 cut(s) 195, 606, 629
DpnII GATC 3 cut(s) 193, 604, 627
Eam1104I CTCTTC 1 cut(s) 769
EarI CTCTTC 1 cut(s) 769
Eco130I CCWWGG 4 cut(s) 322, 466, 583, 744
Eco24I GRGCYC 1 cut(s) 715
Eco31I GGTCTC 1 cut(s) 926
Eco47I GGWCC 3 cut(s) 17, 462, 538
Eco91I GGTNACC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 462
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 4 cut(s) 322, 466, 583, 744
EcoT38I GRGCYC 1 cut(s) 715
ErhI CCWWGG 4 cut(s) 322, 466, 583, 744
FaeI CATG 6 cut(s) 60, 254, 441, 695, 920, 1005
FaqI GGGAC 1 cut(s) 448
FatI CATG 6 cut(s) 56, 250, 437, 691, 916, 1001
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 4 cut(s) 222, 387, 409, 498
FokI GGATG 4 cut(s) 246, 246, 440, 950
FriOI GRGCYC 1 cut(s) 715
Fsp4HI GCNGC 4 cut(s) 222, 387, 409, 498
FspBI CTAG 5 cut(s) 159, 323, 381, 708, 974
GlaI GCGC 1 cut(s) 943
GluI GCNGC 4 cut(s) 222, 387, 409, 498
HapII CCGG 1 cut(s) 240
HhaI GCGC 1 cut(s) 944
Hin1II CATG 6 cut(s) 60, 254, 441, 695, 920, 1005
Hin6I GCGC 1 cut(s) 942
HinP1I GCGC 1 cut(s) 942
HindIII AAGCTT 3 cut(s) 71, 173, 336
HinfI GANTC 3 cut(s) 45, 591, 903
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 2 cut(s) 373, 655
Hpy188I TCNGA 3 cut(s) 169, 198, 647
Hpy188III TCNNGA 3 cut(s) 892, 923, 974
Hpy8I GTNNAC 2 cut(s) 373, 655
HpyAV CCTTC 3 cut(s) 224, 308, 358
HpyCH4III ACNGT 4 cut(s) 94, 106, 358, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 501
Hsp92II CATG 6 cut(s) 60, 254, 441, 695, 920, 1005
HspAI GCGC 1 cut(s) 942
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 3 cut(s) 193, 604, 627
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 1 cut(s) 894
Lsp1109I GCAGC 4 cut(s) 233, 398, 420, 484
LweI GCATC 3 cut(s) 173, 224, 299
MaeI CTAG 5 cut(s) 159, 323, 381, 708, 974
MaeIII GTNAC 5 cut(s) 28, 86, 295, 443, 559
MalI GATC 3 cut(s) 195, 606, 629
MboI GATC 3 cut(s) 193, 604, 627
MboII GAAGA 5 cut(s) 17, 238, 373, 599, 756
MflI RGATCY 2 cut(s) 193, 604
MhlI GDGCHC 1 cut(s) 715
MluCI AATT 7 cut(s) 530, 797, 821, 837, 864, 945, 993
MmeI TCCRAC 1 cut(s) 797
MnlI CCTC 3 cut(s) 155, 582, 744
MseI TTAA 3 cut(s) 344, 680, 912
MslI CAYNNNNRTG 3 cut(s) 635, 696, 738
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 241, 612
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 241
NdeI CATATG 1 cut(s) 490
NdeII GATC 3 cut(s) 193, 604, 627
NlaIII CATG 6 cut(s) 60, 254, 441, 695, 920, 1005
NlaIV GGNNCC 2 cut(s) 319, 464
NmuCI GTSAC 2 cut(s) 28, 443
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 2 cut(s) 97, 729
PciI ACATGT 1 cut(s) 691
PciSI GCTCTTC 1 cut(s) 769
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 3 cut(s) 45, 591, 903
PkrI GCNGC 4 cut(s) 223, 388, 410, 499
PpuMI RGGWCCY 1 cut(s) 462
PscI ACATGT 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 319, 464
PspPI GGNCC 3 cut(s) 17, 462, 538
PspPPI RGGWCCY 1 cut(s) 462
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 193, 604
RsaI GTAC 2 cut(s) 525, 755
RsaNI GTAC 2 cut(s) 524, 754
RseI CAYNNNNRTG 3 cut(s) 635, 696, 738
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 3 cut(s) 344, 680, 912
SatI GCNGC 4 cut(s) 222, 387, 409, 498
Sau3AI GATC 3 cut(s) 193, 604, 627
Sau96I GGNCC 3 cut(s) 17, 462, 538
ScrFI CCNGG 2 cut(s) 241, 612
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 3 cut(s) 173, 224, 299
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 2 cut(s) 97, 729
SinI GGWCC 3 cut(s) 17, 462, 538
SmiMI CAYNNNNRTG 3 cut(s) 635, 696, 738
Sse9I AATT 7 cut(s) 530, 797, 821, 837, 864, 945, 993
SsiI CCGC 2 cut(s) 521, 536
SspI AATATT 2 cut(s) 330, 474
SspMI CTAG 5 cut(s) 159, 323, 381, 708, 974
StyD4I CCNGG 2 cut(s) 239, 610
StyI CCWWGG 4 cut(s) 322, 466, 583, 744
TaaI ACNGT 4 cut(s) 94, 106, 358, 394
TaqI TCGA 6 cut(s) 97, 228, 729, 881, 906, 924
TasI AATT 7 cut(s) 530, 797, 821, 837, 864, 945, 993
TfiI GAWTC 3 cut(s) 45, 591, 903
Tru1I TTAA 3 cut(s) 344, 680, 912
Tru9I TTAA 3 cut(s) 344, 680, 912
TscAI CASTG 2 cut(s) 97, 852
TseFI GTSAC 2 cut(s) 28, 443
TseI GCWGC 4 cut(s) 221, 386, 408, 497
Tsp45I GTSAC 2 cut(s) 28, 443
TspDTI ATGAA 7 cut(s) 37, 83, 300, 426, 474, 583, 899
TspRI CASTG 2 cut(s) 97, 852
VpaK11BI GGWCC 3 cut(s) 17, 462, 538
XapI RAATTY 2 cut(s) 821, 864
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 1 cut(s) 695
XmaJI CCTAGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 5 cut(s) 159, 323, 381, 708, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.