RLG00000028493

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000382
Physical Location & Seq
Reverse (-)
23415185 .. 23417277
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028493

Sequence Viewer

Length: 849 bp
ATGGAAGAGGAAATGGGTCCAGTTTGTGTAACAGGTGGAACTGGATTCATCGGTTCACGACTGGTTATGAAGCTTCTGCAAATTGGTTACACTGTTCGAACTACTGTTAGACCTGATCCAGAAGCTTCACATGATCACATCTTCAATGGGGATCTGAACCAACCTGAAAGTTTTAATGTAGCAATTGAAGGATGCACTGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAACTTGATGAAGCATCGGTAACCAAACGATCTGTAGAAGGTACCCTAGGCATATTGAAAGCTTGCCTAAATGCCAGGACTGTGAAAAAGGTTGTTCAGCAACTTTCATGGAGTGATATAGAGTACCATAGGTCTCTTGGATTATTTAGGAGTTCATCGTATGTGGCTGCTAAGACCAAAACAGAGCAAGCAGTACTGGAATTTGCAGAAAAAAATGGATTAGAACTTGTGACTTTGATCTCTCCATTCGTCCTTGGTGGTTTCATCTGCAAAGGTCTCCCTGGTTCAGTGGAATTGATCTTATCCATGATATTAGGCAATGCGCATATCTTTCTTTTTGAAAATTCTAATGCCAAAGGAAGGTATATTTGCTCTTCAACTCCAACATCAATAGACGGAATGTACCTATTTCTTTCTGCAAAATACCCTGAATTTCACATACCCACAACTCAATCACTGAAAGAAGCTTACAAAATTTGTGGTTTCTCATCGAAGAAGCTCCTGAATACTGGATTCACATTTAAGCATGGCCTCGACGACATATTGACTGGAGCATTTCAATCTTGCAGAGAAAAGGGTTTTCTGGAAAGGTTCTTTGGCAAAATTGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

283

Amino Acids

31.03

Weight (kDa)

7.07

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 166 8.1e-16 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 110 1.3e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 143 8.2e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 558
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 275
AclWI GGATC 2 cut(s) 110, 159
AcsI RAATTY 4 cut(s) 434, 577, 665, 708
AfaI GTAC 4 cut(s) 277, 359, 429, 638
AfiI CCNNNNNNNGG 1 cut(s) 594
AgsI TTSAA 6 cut(s) 145, 188, 292, 575, 612, 794
AjnI CCWGG 2 cut(s) 308, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 813, 845
AluBI AGCT 5 cut(s) 73, 125, 296, 701, 733
AluI AGCT 5 cut(s) 73, 125, 296, 701, 733
Alw26I GTCTC 2 cut(s) 372, 515
AlwI GGATC 2 cut(s) 110, 159
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 4 cut(s) 434, 577, 665, 708
Asp718I GGTACC 1 cut(s) 275
AspA2I CCTAGG 1 cut(s) 280
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 17
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 280
BanI GGYRCC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 310, 516
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 372, 515
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 1 cut(s) 402
BlnI CCTAGG 1 cut(s) 280
BlsI GCNGC 1 cut(s) 403
BmcAI AGTACT 1 cut(s) 429
Bme1390I CCNGG 2 cut(s) 310, 516
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 277
BmrFI CCNGG 2 cut(s) 310, 516
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 2 cut(s) 182, 257
BmuI ACTGGG 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 97
BsaI GGTCTC 2 cut(s) 372, 515
BsaJI CCNNGG 3 cut(s) 280, 487, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse1I ACTGG 7 cut(s) 20, 46, 66, 202, 435, 748, 787
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 2 cut(s) 310, 516
BseDI CCNNGG 3 cut(s) 280, 487, 514
BseGI GGATG 2 cut(s) 197, 217
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMI GCAATG 1 cut(s) 559
BseNI ACTGG 7 cut(s) 20, 46, 66, 202, 435, 748, 787
BseXI GCAGC 1 cut(s) 388
BshFI GGCC 1 cut(s) 765
BshNI GGYRCC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 2 cut(s) 372, 515
BsnI GGCC 1 cut(s) 765
Bso31I GGTCTC 2 cut(s) 372, 515
Bsp119I TTCGAA 1 cut(s) 97
Bsp143I GATC 6 cut(s) 115, 133, 151, 263, 471, 531
BspANI GGCC 1 cut(s) 765
BspLI GGNNCC 2 cut(s) 18, 277
BspPI GGATC 2 cut(s) 110, 159
BspQI GCTCTTC 1 cut(s) 613
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 275
BspTNI GGTCTC 2 cut(s) 372, 515
BsrDI GCAATG 1 cut(s) 559
BsrI ACTGG 7 cut(s) 20, 46, 66, 202, 435, 748, 787
BssECI CCNNGG 3 cut(s) 280, 487, 514
BssMI GATC 6 cut(s) 115, 133, 151, 263, 471, 531
BssT1I CCWWGG 2 cut(s) 280, 487
Bst2UI CCWGG 2 cut(s) 310, 516
Bst4CI ACNGT 3 cut(s) 94, 106, 316
Bst6I CTCTTC 1 cut(s) 613
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 298, 423
BstDEI CTNAG 1 cut(s) 405
BstEII GGTNACC 1 cut(s) 253
BstF5I GGATG 2 cut(s) 197, 217
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 6 cut(s) 118, 136, 154, 266, 474, 534
BstMAI GTCTC 2 cut(s) 372, 515
BstMBI GATC 6 cut(s) 115, 133, 151, 263, 471, 531
BstNI CCWGG 2 cut(s) 310, 516
BstPI GGTNACC 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 308, 514
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 765
BtsCI GGATG 2 cut(s) 197, 217
BtsIMutI CAGTG 4 cut(s) 90, 195, 528, 689
Cac8I GCNNGC 2 cut(s) 298, 423
CfoI GCGC 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 4 cut(s) 276, 358, 428, 637
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 6 cut(s) 131, 209, 342, 541, 761, 846
CviJI RGCY 7 cut(s) 73, 125, 296, 401, 701, 733, 765
CviKI_1 RGCY 7 cut(s) 73, 125, 296, 401, 701, 733, 765
CviQI GTAC 4 cut(s) 276, 358, 428, 637
DdeI CTNAG 1 cut(s) 405
DpnI GATC 6 cut(s) 117, 135, 153, 265, 473, 533
DpnII GATC 6 cut(s) 115, 133, 151, 263, 471, 531
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
Eco130I CCWWGG 2 cut(s) 280, 487
Eco31I GGTCTC 2 cut(s) 372, 515
Eco47I GGWCC 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 308, 514
EcoT14I CCWWGG 2 cut(s) 280, 487
ErhI CCWWGG 2 cut(s) 280, 487
FaeI CATG 6 cut(s) 134, 212, 345, 544, 764, 849
FatI CATG 6 cut(s) 130, 208, 341, 540, 760, 845
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 2 cut(s) 204, 204
Fsp4HI GCNGC 1 cut(s) 402
FspAI RTGCGCAY 1 cut(s) 558
FspBI CTAG 1 cut(s) 281
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 1 cut(s) 558
GluI GCNGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 804
HaeIII GGCC 1 cut(s) 765
HhaI GCGC 1 cut(s) 559
Hin1II CATG 6 cut(s) 134, 212, 345, 544, 764, 849
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HindIII AAGCTT 4 cut(s) 71, 123, 294, 699
HinfI GANTC 2 cut(s) 45, 747
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 4 cut(s) 57, 119, 736, 818
Hpy8I GTNNAC 1 cut(s) 56
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 3 cut(s) 182, 266, 588
HpyCH4III ACNGT 3 cut(s) 94, 106, 316
HpyCH4V TGCA 6 cut(s) 79, 195, 440, 504, 653, 801
HpyF3I CTNAG 1 cut(s) 405
Hsp92II CATG 6 cut(s) 134, 212, 345, 544, 764, 849
HspAI GCGC 1 cut(s) 557
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 6 cut(s) 115, 133, 151, 263, 471, 531
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 2 cut(s) 738, 785
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 2 cut(s) 182, 257
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 4 cut(s) 28, 86, 253, 463
MalI GATC 6 cut(s) 117, 135, 153, 265, 473, 533
MboI GATC 6 cut(s) 115, 133, 151, 263, 471, 531
MboII GAAGA 5 cut(s) 17, 133, 196, 600, 739
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 151
MluCI AATT 8 cut(s) 81, 183, 434, 527, 577, 665, 708, 837
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 1 cut(s) 776
MseI TTAA 2 cut(s) 174, 756
MspR9I CCNGG 2 cut(s) 310, 516
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 2 cut(s) 310, 516
NdeII GATC 6 cut(s) 115, 133, 151, 263, 471, 531
NlaIII CATG 6 cut(s) 134, 212, 345, 544, 764, 849
NlaIV GGNNCC 2 cut(s) 18, 277
NmuCI GTSAC 1 cut(s) 463
NsbI TGCGCA 1 cut(s) 558
NspV TTCGAA 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 45, 747
PkrI GCNGC 1 cut(s) 403
Psp6I CCWGG 2 cut(s) 308, 514
PspEI GGTNACC 1 cut(s) 253
PspGI CCWGG 2 cut(s) 308, 514
PspN4I GGNNCC 2 cut(s) 18, 277
PspPI GGNCC 1 cut(s) 17
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 4 cut(s) 277, 359, 429, 638
RsaNI GTAC 4 cut(s) 276, 358, 428, 637
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 2 cut(s) 174, 756
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 6 cut(s) 115, 133, 151, 263, 471, 531
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 310, 516
SfaNI GCATC 2 cut(s) 182, 257
SfcI CTRYAG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 17
Sse9I AATT 8 cut(s) 81, 183, 434, 527, 577, 665, 708, 837
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 308, 514
StyI CCWWGG 2 cut(s) 280, 487
TaaI ACNGT 3 cut(s) 94, 106, 316
TaqI TCGA 3 cut(s) 97, 725, 768
TasI AATT 8 cut(s) 81, 183, 434, 527, 577, 665, 708, 837
TatI WGTACW 1 cut(s) 427
TfiI GAWTC 2 cut(s) 45, 747
Tru1I TTAA 2 cut(s) 174, 756
Tru9I TTAA 2 cut(s) 174, 756
TscAI CASTG 4 cut(s) 97, 202, 528, 696
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 6 cut(s) 37, 83, 258, 330, 378, 487
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 4 cut(s) 97, 202, 528, 696
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 4 cut(s) 434, 577, 665, 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmaJI CCTAGG 1 cut(s) 280
XspI CTAG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.