Rh1BG193400

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000382
Physical Location & Seq
Forward (+)
30983709 .. 30987872
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG193400.1

Sequence Viewer

Length: 981 bp
ATGGATGAAGACAAGGGTCCAGTTTGTGTAACAGGTGGAGCTGGATTCATCGGCTCATGGCTGGTTATGAAGCTTCTGCAACATGGTTACACTGTTCGAACTACTGTTAGACCTGACCCAGAATGCAAGAGAGACATCAGCTACCTCACAAGCCTACCAGGAGCATCAGAGAAGCTTCACATCTTTAGTGCAGATCTCGACCAACCTGAAAGTTTCAATGCAGCAATTGAAGGATGCATTGGAGTCTTTCATGTTGCTCATCCTAATCCTCTGAAAGAACAAGATGAAGAAATTGTAACCAAAAAATCAGTAGAAGGCACCCTAGGCATATTGAGAGCCTGCCTGAATTCCAAGACTGTGAAGAGGGTTGTATACACTTCTAGTGCATCAACTATTTCTCATAGTGGAACCAACAAAGACATGGTGGATGAGAGTACATGGACTGACGTTGAATTTCATAGGTCTCTGAAAGTATATGGGACTTCATATGTGGCTGCGAAAACCAAGACAGAGCAAGCAGCTCTGGAATTTGGGGAAAATAATGGACTGGAAATAGTCACTTTGATCCCTCCATTAGTGATTGGTGGATTTCTCTGCAAAACACTCCCTAGCTCAATAAGTCTGTTCCTATGCATGATTCTAGGAAATGAGCATCACTATGAACATCTGAAAAACAAAAGTATGGTACACTTAGATGATTTGGTCAGTGCACATATCTTTCTTTTCGAAAACCCTAATGCAAAAGGGAGGTACATTTGTTCATTAGCTCAGGTATCCTTAGAGGAAATGTCTCGATTTCTCACTGCAAAATACCCTGAATATCAAATACCGACAGCTGATTCTTTGAAGCGCATTGAAGGTCACAAAACGTGTGGGTACTCATCGGAGAAGCTCCTGAAGTCTGGATTCAAATTTAAGCATGGGCTCGAAGACATGTTTGAGGGAGCAGTTCAATCTTGCAGAGAAAAGGGATTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

326

Amino Acids

36.09

Weight (kDa)

6.23

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 135 3.6e-07 NmrA-like family
Epimerase PF01370 8 - 248 2.7e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 188 2.4e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 261 6.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 195 1.7e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 133 2e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 372
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 4 cut(s) 346, 452, 527, 911
AcuI CTGAAG 1 cut(s) 917
AfaI GTAC 4 cut(s) 436, 687, 752, 878
AflIII ACRYGT 2 cut(s) 869, 933
AgsI TTSAA 7 cut(s) 217, 230, 452, 847, 857, 910, 953
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 9 cut(s) 41, 73, 141, 175, 521, 612, 767, 836, 892
AluI AGCT 9 cut(s) 41, 73, 141, 175, 521, 612, 767, 836, 892
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 3 cut(s) 126, 468, 795
Alw44I GTGCAC 1 cut(s) 708
AlwI GGATC 1 cut(s) 559
ApaLI GTGCAC 1 cut(s) 708
ApeKI GCWGC 3 cut(s) 221, 494, 518
ApoI RAATTY 4 cut(s) 346, 452, 527, 911
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 1 cut(s) 852
AspS9I GGNCC 1 cut(s) 17
AsuII TTCGAA 2 cut(s) 97, 726
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 712
BaeI ACNNNNGTAYC 2 cut(s) 742, 775
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 927
BbsI GAAGAC 2 cut(s) 15, 936
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 3 cut(s) 233, 481, 530
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 1 cut(s) 784
BcoDI GTCTC 3 cut(s) 126, 468, 795
BfaI CTAG 4 cut(s) 323, 381, 609, 641
BfuI GTATCC 1 cut(s) 784
BglII AGATCT 1 cut(s) 193
BisI GCNGC 3 cut(s) 222, 495, 519
BlnI CCTAGG 1 cut(s) 322
BlsI GCNGC 3 cut(s) 223, 496, 520
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 3 cut(s) 18, 319, 409
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 4 cut(s) 173, 224, 395, 661
BoxI GACNNNNGTC 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 15, 936
Bpu10I CCTNAGC 1 cut(s) 768
Bpu14I TTCGAA 2 cut(s) 97, 726
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 20, 552
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 4 cut(s) 10, 239, 259, 433
BseMII CTCAG 1 cut(s) 782
BseNI ACTGG 2 cut(s) 20, 552
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 3 cut(s) 233, 481, 530
BsgI GTGCAG 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 712
BslFI GGGAC 1 cut(s) 493
BsmAI GTCTC 3 cut(s) 126, 468, 795
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 468
Bsp119I TTCGAA 2 cut(s) 97, 726
Bsp1286I GDGCHC 2 cut(s) 712, 927
Bsp143I GATC 2 cut(s) 193, 564
BspCNI CTCAG 1 cut(s) 781
BspLI GGNNCC 3 cut(s) 18, 319, 409
BspPI GGATC 1 cut(s) 559
BspT104I TTCGAA 2 cut(s) 97, 726
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 468
BsrI ACTGG 2 cut(s) 20, 552
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 2 cut(s) 193, 564
BssNAI GTATAC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 322
Bst1107I GTATAC 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 3 cut(s) 94, 106, 358
Bst6I CTCTTC 1 cut(s) 356
BstBI TTCGAA 2 cut(s) 97, 726
BstC8I GCNNGC 2 cut(s) 340, 516
BstDEI CTNAG 3 cut(s) 691, 768, 778
BstF5I GGATG 4 cut(s) 10, 239, 259, 433
BstHHI GCGC 1 cut(s) 852
BstKTI GATC 2 cut(s) 196, 567
BstMAI GTCTC 3 cut(s) 126, 468, 795
BstMBI GATC 2 cut(s) 193, 564
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 937
BstPAI GACNNNNGTC 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 3 cut(s) 233, 481, 530
BstV2I GAAGAC 2 cut(s) 15, 936
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BstZ17I GTATAC 1 cut(s) 373
BsuI GTATCC 1 cut(s) 784
BtsCI GGATG 4 cut(s) 10, 239, 259, 433
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 3 cut(s) 90, 712, 801
Cac8I GCNNGC 2 cut(s) 340, 516
CfoI GCGC 1 cut(s) 852
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 4 cut(s) 435, 686, 751, 877
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 8 cut(s) 57, 83, 251, 421, 438, 634, 920, 934
CviQI GTAC 4 cut(s) 435, 686, 751, 877
DdeI CTNAG 3 cut(s) 691, 768, 778
DpnI GATC 2 cut(s) 195, 566
DpnII GATC 2 cut(s) 193, 564
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 927
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 917
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 322
EcoT22I ATGCAT 2 cut(s) 239, 635
EcoT38I GRGCYC 1 cut(s) 927
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 8 cut(s) 60, 86, 254, 424, 441, 637, 923, 937
FaqI GGGAC 1 cut(s) 493
FatI CATG 8 cut(s) 56, 82, 250, 420, 437, 633, 919, 933
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 222, 495, 519
FokI GGATG 4 cut(s) 17, 246, 246, 440
FriOI GRGCYC 1 cut(s) 927
Fsp4HI GCNGC 3 cut(s) 222, 495, 519
FspBI CTAG 4 cut(s) 323, 381, 609, 641
GlaI GCGC 1 cut(s) 851
GluI GCNGC 3 cut(s) 222, 495, 519
HhaI GCGC 1 cut(s) 852
Hin1II CATG 8 cut(s) 60, 86, 254, 424, 441, 637, 923, 937
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HindIII AAGCTT 2 cut(s) 71, 173
HinfI GANTC 5 cut(s) 45, 243, 637, 839, 906
Hpy166II GTNNAC 3 cut(s) 373, 688, 710
Hpy188I TCNGA 5 cut(s) 169, 273, 468, 669, 886
Hpy188III TCNNGA 5 cut(s) 197, 524, 792, 895, 903
Hpy8I GTNNAC 3 cut(s) 373, 688, 710
HpyAV CCTTC 3 cut(s) 224, 308, 851
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4IV ACGT 2 cut(s) 447, 869
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 3 cut(s) 691, 768, 778
HpySE526I ACGT 2 cut(s) 447, 869
Hsp92II CATG 8 cut(s) 60, 86, 254, 424, 441, 637, 923, 937
HspAI GCGC 1 cut(s) 850
Kzo9I GATC 2 cut(s) 193, 564
LmnI GCTCC 4 cut(s) 38, 161, 897, 944
Lsp1109I GCAGC 3 cut(s) 233, 481, 530
LweI GCATC 4 cut(s) 173, 224, 395, 661
MaeI CTAG 4 cut(s) 323, 381, 609, 641
MaeII ACGT 2 cut(s) 447, 869
MaeIII GTNAC 5 cut(s) 28, 86, 295, 556, 860
MalI GATC 2 cut(s) 195, 566
MboI GATC 2 cut(s) 193, 564
MboII GAAGA 4 cut(s) 20, 299, 373, 941
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 712, 927
MluCI AATT 6 cut(s) 225, 291, 346, 452, 527, 911
MlyI GAGTC 1 cut(s) 252
MnlI CCTC 7 cut(s) 155, 279, 357, 579, 741, 775, 934
Mph1103I ATGCAT 2 cut(s) 239, 635
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 2 cut(s) 657, 693
MspA1I CMGCKG 1 cut(s) 836
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeI CATATG 1 cut(s) 487
NdeII GATC 2 cut(s) 193, 564
NlaIII CATG 8 cut(s) 60, 86, 254, 424, 441, 637, 923, 937
NlaIV GGNNCC 3 cut(s) 18, 319, 409
NmuCI GTSAC 2 cut(s) 556, 860
NsiI ATGCAT 2 cut(s) 239, 635
NspI RCATGY 1 cut(s) 937
NspV TTCGAA 2 cut(s) 97, 726
PciI ACATGT 1 cut(s) 933
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 4 cut(s) 45, 637, 839, 906
PkrI GCNGC 3 cut(s) 223, 496, 520
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PscI ACATGT 1 cut(s) 933
PshAI GACNNNNGTC 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 18, 319, 409
PspPI GGNCC 1 cut(s) 17
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 4 cut(s) 436, 687, 752, 878
RsaNI GTAC 4 cut(s) 435, 686, 751, 877
RseI CAYNNNNRTG 2 cut(s) 657, 693
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 3 cut(s) 222, 495, 519
Sau3AI GATC 2 cut(s) 193, 564
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 2 cut(s) 712, 927
SfaNI GCATC 4 cut(s) 173, 224, 395, 661
SfuI TTCGAA 2 cut(s) 97, 726
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 2 cut(s) 657, 693
Sse9I AATT 6 cut(s) 225, 291, 346, 452, 527, 911
SspMI CTAG 4 cut(s) 323, 381, 609, 641
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 94, 106, 358
TaiI ACGT 2 cut(s) 450, 872
TaqI TCGA 5 cut(s) 97, 198, 726, 793, 927
TasI AATT 6 cut(s) 225, 291, 346, 452, 527, 911
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 4 cut(s) 45, 637, 839, 906
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 3 cut(s) 97, 712, 808
TseFI GTSAC 2 cut(s) 556, 860
TseI GCWGC 3 cut(s) 221, 494, 518
Tsp45I GTSAC 2 cut(s) 556, 860
TspDTI ATGAA 9 cut(s) 21, 37, 83, 239, 300, 446, 474, 675, 750
TspRI CASTG 3 cut(s) 97, 712, 808
VneI GTGCAC 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 4 cut(s) 346, 452, 527, 911
XceI RCATGY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmaJI CCTAGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 323, 381, 609, 641
Zsp2I ATGCAT 2 cut(s) 239, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.