Rh1AG225600

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000382
Physical Location & Seq
Forward (+)
42909877 .. 42923404
13528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG225600.1

Sequence Viewer

Length: 687 bp
ATGGAAGAGGAAAAGGGTCCAGTTTGTGTAACGGGTGGAACTGGATTCATCGGTTCATGGCTGGTTATGAAGCTTCTGCAAAATGGTTACACTGTTCGAACTACTGTTAGACCTGATCCAAAATGCAAGAGAGACATCAACTACCTCACAAGCCTGCCTAGAGCATCGGAGAAGCTTCACATCTTCAATGGGGATCTGAACCAACCTGAAAGTTTTAATGTAGCAATTGAAGGATGCACCGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAATTTGATGAAGCATCGGTAACCAAACAATCTGTAGAAGGTACCCTAGGCATATTGAAAGCTTGCCTAAATGCCAAGACTGTGAAAAAGGTTGTTTACACTTCTAGTGCAGCAACTGTTGCTTATAGTGGCAACAACAAAGACATGGCGGATGAGAGTTCATGGAGTGATATAGAGTACCATAGGTCTCTTGGATTATTTAGGAGTTCATCGTATGTGGCTGCTAAGACCAAAACAGAGCAAGCAGTACTGGAATTTGCAGAAAAAAATGGATTAGAACTTGTGACTTTGATCCCTCCATTCGTCCTGGTGGTTTCATCTGCAAAGATCTCCCTGATCAAGAACGCTCGCAAAGATCTACAACGATGGGGAGAAGGGTTTTGGGCGGCGGAGCAGTGGTGGAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

228

Amino Acids

25.35

Weight (kDa)

8.27

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 135 4e-09 NmrA-like family
Epimerase PF01370 8 - 192 8.4e-20 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 138 1.2e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 188 1.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 190 2.7e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 197 9.5e-14 Male sterility protein
NAD_binding_10 PF13460 12 - 132 2.3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 3 cut(s) 425, 662, 665
AclWI GGATC 3 cut(s) 110, 201, 562
AcsI RAATTY 2 cut(s) 278, 530
AfaI GTAC 3 cut(s) 319, 455, 525
AgsI TTSAA 3 cut(s) 187, 230, 334
AjnI CCWGG 1 cut(s) 582
AluBI AGCT 3 cut(s) 73, 175, 338
AluI AGCT 3 cut(s) 73, 175, 338
Alw26I GTCTC 2 cut(s) 126, 468
AlwI GGATC 3 cut(s) 110, 201, 562
AlwNI CAGNNNCTG 1 cut(s) 392
ApeKI GCWGC 2 cut(s) 386, 497
ApoI RAATTY 2 cut(s) 278, 530
Asp718I GGTACC 1 cut(s) 317
AspA2I CCTAGG 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 322
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 398, 484
BccI CCATC 1 cut(s) 636
BciT130I CCWGG 1 cut(s) 584
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 126, 468
BfaI CTAG 3 cut(s) 159, 323, 381
BfmI CTRYAG 1 cut(s) 309
BglII AGATCT 2 cut(s) 603, 631
BisI GCNGC 3 cut(s) 387, 498, 663
BlnI CCTAGG 1 cut(s) 322
BlsI GCNGC 3 cut(s) 388, 499, 664
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 241, 584
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 319
BmrFI CCNGG 2 cut(s) 241, 584
BmsI GCATC 3 cut(s) 173, 224, 299
BpiI GAAGAC 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 240, 322
BsaXI ACNNNNNCTCC 1 cut(s) 659
Bse1I ACTGG 3 cut(s) 20, 46, 531
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 240, 322
BseGI GGATG 3 cut(s) 239, 259, 433
BseNI ACTGG 3 cut(s) 20, 46, 531
BseXI GCAGC 2 cut(s) 398, 484
BsgI GTGCAG 1 cut(s) 405
BshNI GGYRCC 1 cut(s) 317
BsiSI CCGG 1 cut(s) 240
BsmAI GTCTC 2 cut(s) 126, 468
Bso31I GGTCTC 1 cut(s) 468
Bsp119I TTCGAA 1 cut(s) 97
Bsp143I GATC 6 cut(s) 115, 193, 567, 603, 612, 631
BspACI CCGC 3 cut(s) 425, 662, 665
BspLI GGNNCC 2 cut(s) 18, 319
BspPI GGATC 3 cut(s) 110, 201, 562
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 468
BsrI ACTGG 3 cut(s) 20, 46, 531
BssECI CCNNGG 2 cut(s) 240, 322
BssMI GATC 6 cut(s) 115, 193, 567, 603, 612, 631
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 4 cut(s) 94, 106, 358, 394
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 155, 340, 519, 625
BstDEI CTNAG 1 cut(s) 501
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 3 cut(s) 239, 259, 433
BstKTI GATC 6 cut(s) 118, 196, 570, 606, 615, 634
BstMAI GTCTC 2 cut(s) 126, 468
BstMBI GATC 6 cut(s) 115, 193, 567, 603, 612, 631
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 584
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 239, 582
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 398, 484
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 193, 603, 631
BstYI RGATCY 3 cut(s) 193, 603, 631
BtsCI GGATG 3 cut(s) 239, 259, 433
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 2 cut(s) 90, 677
Cac8I GCNNGC 4 cut(s) 155, 340, 519, 625
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 3 cut(s) 318, 454, 524
CviAII CATG 4 cut(s) 57, 251, 421, 438
CviJI RGCY 6 cut(s) 61, 73, 153, 175, 338, 497
CviKI_1 RGCY 6 cut(s) 61, 73, 153, 175, 338, 497
CviQI GTAC 3 cut(s) 318, 454, 524
DdeI CTNAG 1 cut(s) 501
DpnI GATC 6 cut(s) 117, 195, 569, 605, 614, 633
DpnII GATC 6 cut(s) 115, 193, 567, 603, 612, 631
EciI GGCGGA 2 cut(s) 440, 680
Eco130I CCWWGG 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 582
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 4 cut(s) 60, 254, 424, 441
FatI CATG 4 cut(s) 56, 250, 420, 437
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 387, 498, 663
FokI GGATG 3 cut(s) 246, 246, 440
Fsp4HI GCNGC 3 cut(s) 387, 498, 663
FspBI CTAG 3 cut(s) 159, 323, 381
GluI GCNGC 3 cut(s) 387, 498, 663
HapII CCGG 1 cut(s) 240
Hin1II CATG 4 cut(s) 60, 254, 424, 441
HindIII AAGCTT 3 cut(s) 71, 173, 336
HinfI GANTC 1 cut(s) 45
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 169, 198
Hpy188III TCNNGA 1 cut(s) 616
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 3 cut(s) 224, 308, 644
HpyCH4III ACNGT 4 cut(s) 94, 106, 358, 394
HpyCH4V TGCA 6 cut(s) 79, 126, 237, 386, 536, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 501
Hsp92II CATG 4 cut(s) 60, 254, 424, 441
KpnI GGTACC 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 6 cut(s) 115, 193, 567, 603, 612, 631
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 398, 484
LweI GCATC 3 cut(s) 173, 224, 299
MaeI CTAG 3 cut(s) 159, 323, 381
MaeIII GTNAC 4 cut(s) 28, 86, 295, 559
MalI GATC 6 cut(s) 117, 195, 569, 605, 614, 633
MboI GATC 6 cut(s) 115, 193, 567, 603, 612, 631
MboII GAAGA 3 cut(s) 17, 175, 238
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 3 cut(s) 193, 603, 631
MluCI AATT 3 cut(s) 225, 278, 530
MnlI CCTC 3 cut(s) 155, 582, 672
MseI TTAA 1 cut(s) 216
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 241, 584
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 241
NdeII GATC 6 cut(s) 115, 193, 567, 603, 612, 631
NlaIII CATG 4 cut(s) 60, 254, 424, 441
NlaIV GGNNCC 2 cut(s) 18, 319
NmuCI GTSAC 1 cut(s) 559
NspV TTCGAA 1 cut(s) 97
PfeI GAWTC 1 cut(s) 45
PkrI GCNGC 3 cut(s) 388, 499, 664
Psp6I CCWGG 1 cut(s) 582
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 2 cut(s) 18, 319
PspPI GGNCC 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 3 cut(s) 193, 603, 631
RsaI GTAC 3 cut(s) 319, 455, 525
RsaNI GTAC 3 cut(s) 318, 454, 524
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 3 cut(s) 387, 498, 663
Sau3AI GATC 6 cut(s) 115, 193, 567, 603, 612, 631
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 525
ScrFI CCNGG 2 cut(s) 241, 584
SfaNI GCATC 3 cut(s) 173, 224, 299
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 17
Sse9I AATT 3 cut(s) 225, 278, 530
SsiI CCGC 3 cut(s) 425, 662, 665
SspMI CTAG 3 cut(s) 159, 323, 381
StyD4I CCNGG 2 cut(s) 239, 582
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 4 cut(s) 94, 106, 358, 394
TaqI TCGA 1 cut(s) 97
TasI AATT 3 cut(s) 225, 278, 530
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 665
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 2 cut(s) 97, 677
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 386, 497
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 7 cut(s) 37, 45, 83, 300, 426, 474, 582
TspRI CASTG 2 cut(s) 97, 677
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 278, 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmaJI CCTAGG 1 cut(s) 322
XspI CTAG 3 cut(s) 159, 323, 381
ZrmI AGTACT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.