pycom08g20120

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000382
Physical Location & Seq
Reverse (-)
19990931 .. 19993953
3023 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20120.4

Sequence Viewer

Length: 1077 bp
ATGAAAACTAGCGGTTCGATATCAGAAAAGAGAGGGCAAGATTGGCAGGGGAGAGAGAGAGAGAGAAAGAGAGAGATGGAAGGAGAGAGATCAGGAAGAGTTGTATGTGTAACAGGTGGTTCAGGGTTCTTAGGGTCTTGGCTGGTTATGAGGCTTCTTGATCGATCCAAAGGCTACTCTGTGAATACTACTGTTAGATCACACCCTGCAGAGAACCAGAGAGATCTAAGCTACCTCACAAGTCTACCAGGAGCAACCGAAAGGCTCAAAATTTTCACAGCGGATCTCAGCAACCCGGACAGCTTCAATGCAGCCGTTGAAGGATGCATCGGAGTGTTCCATGTTGCCACTCCAGTCGATTTCCAAGACCAGGAACCAGAACAAGTAGTGACCAAAAGATCAATTGATGGAGCTCTCGGCATTCTGAAGGCGTGCTTAAATGCAAAAACAGTGAAACGAGTTGTGTACACTTCTAGTGCATCCGCTGTCGTGTTTGGCAGCGGCAATGATGTGGAGGAAGTGGATGAAAGTTTTTGGAGCGACGTAGATTACATTAAAGCTAACATTTCATATGGAGGGTCATACATGATTTCAAAGACATTAACTGAGAAGGCAGTTCTTGAATTTTCAGAGAAATATGGGTTGGATGTTGTGACAGTAATACCTTCTTTTGTTGTTGGCCCCTTCATTTGTCCTAAGCTTCCCGGCTCCGTTCACAGTGCACTGGCTCTGGTCTTTGGTAACCTTGATAAGCTTAGCTTTCATATTAATACGCCTATGGTGCATGTCGATGATGTTGCCAGAGCGCACATTTTCCTTTTCGAACATCATAATGCAAAAGGGAGGTACAATTGCTCATCACATGTCATAACCCTTGTACAAATGGCTGAGTTTCTTTCTGCCAAATACCCGGAGTTCCAAATACCCTCAGTAAGCTATTTAAGGGAGGTTAAAGGTGAGAAAGCACCTGGTCTGTCGTCAAAGAAGCTCTTGGATTCAGGTTTCAGATTCAAGTATGGGGTTGATGAGATGCTCATCGATGCAATTCAATGCTGCAAAGAAAAGCATTATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

359

Amino Acids

39.61

Weight (kDa)

6.8

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 244
AciI CCGC 4 cut(s) 12, 281, 483, 501
AclWI GGATC 2 cut(s) 159, 291
AcsI RAATTY 2 cut(s) 270, 623
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 3 cut(s) 467, 848, 879
AfiI CCNNNNNNNGG 1 cut(s) 370
AflIII ACRYGT 1 cut(s) 862
AgsI TTSAA 6 cut(s) 307, 320, 594, 623, 1012, 1049
AjnI CCWGG 3 cut(s) 247, 369, 967
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AluBI AGCT 9 cut(s) 231, 303, 413, 560, 700, 754, 759, 936, 988
AluI AGCT 9 cut(s) 231, 303, 413, 560, 700, 754, 759, 936, 988
Alw21I GWGCWC 2 cut(s) 415, 724
Alw44I GTGCAC 1 cut(s) 720
AlwI GGATC 2 cut(s) 159, 291
AoxI GGCC 1 cut(s) 679
ApaLI GTGCAC 1 cut(s) 720
ApeKI GCWGC 3 cut(s) 311, 498, 1053
ApoI RAATTY 2 cut(s) 270, 623
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 1 cut(s) 680
AsuC2I CCSGG 3 cut(s) 296, 705, 911
AsuHPI GGTGA 1 cut(s) 968
AsuII TTCGAA 1 cut(s) 822
BaeGI GKGCMC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 2 cut(s) 415, 724
BbvI GCAGC 3 cut(s) 323, 510, 1040
BccI CCATC 2 cut(s) 70, 401
BceAI ACGGC 1 cut(s) 299
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 3 cut(s) 249, 371, 969
BcnI CCSGG 3 cut(s) 296, 705, 911
BfaI CTAG 2 cut(s) 9, 474
BfmI CTRYAG 1 cut(s) 207
BglII AGATCT 1 cut(s) 223
BisI GCNGC 4 cut(s) 312, 499, 502, 1054
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 4 cut(s) 313, 500, 503, 1055
Bme1390I CCNGG 6 cut(s) 249, 296, 371, 705, 911, 969
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 3 cut(s) 375, 682, 709
BmrFI CCNGG 6 cut(s) 249, 296, 371, 705, 911, 969
BmsI GCATC 5 cut(s) 314, 336, 488, 1020, 1030
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 696
Bpu1102I GCTNAGC 1 cut(s) 755
Bpu14I TTCGAA 1 cut(s) 822
BpuMI CCSGG 3 cut(s) 296, 705, 911
Bsa29I ATCGAT 2 cut(s) 163, 1038
BsaBI GATNNNNATC 1 cut(s) 1034
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 2 cut(s) 353, 729
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 1034
BseBI CCWGG 3 cut(s) 249, 371, 969
BseCI ATCGAT 2 cut(s) 163, 1038
BseGI GGATG 4 cut(s) 329, 479, 529, 652
BseJI GATNNNNATC 1 cut(s) 1034
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 4 cut(s) 301, 597, 879, 942
BseNI ACTGG 2 cut(s) 353, 729
BseSI GKGCMC 1 cut(s) 724
BseXI GCAGC 3 cut(s) 323, 510, 1040
BshFI GGCC 1 cut(s) 681
BshVI ATCGAT 2 cut(s) 163, 1038
BsiHKAI GWGCWC 2 cut(s) 415, 724
BsiSI CCGG 3 cut(s) 296, 705, 911
BslI CCNNNNNNNGG 1 cut(s) 370
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 1 cut(s) 681
Bsp119I TTCGAA 1 cut(s) 822
Bsp1286I GDGCHC 2 cut(s) 415, 724
Bsp1407I TGTACA 2 cut(s) 465, 877
Bsp143I GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 4 cut(s) 12, 281, 483, 501
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 4 cut(s) 300, 598, 880, 941
BspDI ATCGAT 2 cut(s) 163, 1038
BspLI GGNNCC 3 cut(s) 375, 682, 709
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 2 cut(s) 159, 291
BspT104I TTCGAA 1 cut(s) 822
BsrDI GCAATG 1 cut(s) 511
BsrGI TGTACA 2 cut(s) 465, 877
BsrI ACTGG 2 cut(s) 353, 729
BssMI GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
Bst2UI CCWGG 3 cut(s) 249, 371, 969
Bst4CI ACNGT 4 cut(s) 193, 451, 658, 719
Bst6I CTCTTC 1 cut(s) 91
BstAUI TGTACA 2 cut(s) 465, 877
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 8 cut(s) 130, 227, 287, 606, 696, 755, 888, 928
BstEII GGTNACC 1 cut(s) 740
BstF5I GGATG 4 cut(s) 329, 479, 529, 652
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 7 cut(s) 92, 163, 167, 200, 226, 286, 401
BstMBI GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
BstMWI GCNNNNNNNGC 2 cut(s) 43, 781
BstNI CCWGG 3 cut(s) 249, 371, 969
BstNSI RCATGY 2 cut(s) 788, 866
BstPI GGTNACC 1 cut(s) 740
BstSCI CCNGG 6 cut(s) 247, 294, 369, 703, 909, 967
BstSFI CTRYAG 1 cut(s) 207
BstSLI GKGCMC 1 cut(s) 724
BstV1I GCAGC 3 cut(s) 323, 510, 1040
BstX2I RGATCY 2 cut(s) 223, 283
BstYI RGATCY 2 cut(s) 223, 283
Bsu15I ATCGAT 2 cut(s) 163, 1038
BsuRI GGCC 1 cut(s) 681
BsuTUI ATCGAT 2 cut(s) 163, 1038
BtsCI GGATG 4 cut(s) 329, 479, 529, 652
BtsIMutI CAGTG 3 cut(s) 456, 722, 724
Cac8I GCNNGC 1 cut(s) 433
CfoI GCGC 1 cut(s) 808
Cfr13I GGNCC 1 cut(s) 680
ClaI ATCGAT 2 cut(s) 163, 1038
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 466, 847, 878
CviAII CATG 4 cut(s) 341, 586, 785, 863
CviQI GTAC 3 cut(s) 466, 847, 878
DdeI CTNAG 8 cut(s) 130, 227, 287, 606, 696, 755, 888, 928
DpnI GATC 7 cut(s) 91, 162, 166, 199, 225, 285, 400
DpnII GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 1 cut(s) 446
Eco91I GGTNACC 1 cut(s) 740
EcoICRI GAGCTC 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 740
EcoRII CCWGG 3 cut(s) 247, 369, 967
EcoRV GATATC 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 329
EcoT38I GRGCYC 1 cut(s) 415
FaeI CATG 4 cut(s) 344, 589, 788, 866
FalI AAGNNNNNCTT 6 cut(s) 419, 451, 743, 775, 974, 1006
FatI CATG 4 cut(s) 340, 585, 784, 862
FauNDI CATATG 1 cut(s) 571
FblI GTMKAC 1 cut(s) 244
Fnu4HI GCNGC 4 cut(s) 312, 499, 502, 1054
FokI GGATG 4 cut(s) 336, 466, 536, 659
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 4 cut(s) 312, 499, 502, 1054
FspBI CTAG 2 cut(s) 9, 474
GlaI GCGC 1 cut(s) 807
GluI GCNGC 4 cut(s) 312, 499, 502, 1054
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 1 cut(s) 681
HapII CCGG 3 cut(s) 296, 705, 911
HhaI GCGC 1 cut(s) 808
Hin1II CATG 4 cut(s) 344, 589, 788, 866
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HindIII AAGCTT 2 cut(s) 698, 752
HinfI GANTC 2 cut(s) 995, 1008
HpaII CCGG 3 cut(s) 296, 705, 911
HphI GGTGA 1 cut(s) 968
Hpy166II GTNNAC 5 cut(s) 245, 466, 468, 715, 722
Hpy188I TCNGA 5 cut(s) 25, 332, 426, 631, 1007
Hpy188III TCNNGA 3 cut(s) 93, 158, 620
Hpy8I GTNNAC 5 cut(s) 245, 466, 468, 715, 722
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 6 cut(s) 74, 314, 421, 604, 675, 694
HpyCH4III ACNGT 4 cut(s) 193, 451, 658, 719
HpyCH4IV ACGT 1 cut(s) 543
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 781
HpyF3I CTNAG 8 cut(s) 130, 227, 287, 606, 696, 755, 888, 928
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 4 cut(s) 344, 589, 788, 866
HspAI GCGC 1 cut(s) 806
Kzo9I GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
LmnI GCTCC 4 cut(s) 251, 410, 537, 713
Lsp1109I GCAGC 3 cut(s) 323, 510, 1040
LweI GCATC 5 cut(s) 314, 336, 488, 1020, 1030
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 2 cut(s) 9, 474
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 109, 388, 652, 740
MalI GATC 7 cut(s) 91, 162, 166, 199, 225, 285, 400
MboI GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
MboII GAAGA 1 cut(s) 108
MfeI CAATTG 2 cut(s) 402, 850
MflI RGATCY 2 cut(s) 223, 283
MhlI GDGCHC 2 cut(s) 415, 724
MluCI AATT 5 cut(s) 270, 402, 623, 850, 1044
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 8 cut(s) 26, 144, 245, 508, 569, 837, 937, 940
Mph1103I ATGCAT 1 cut(s) 329
MseI TTAA 6 cut(s) 437, 555, 602, 768, 941, 951
MslI CAYNNNNRTG 3 cut(s) 332, 789, 831
MspA1I CMGCKG 3 cut(s) 281, 485, 501
MspI CCGG 3 cut(s) 296, 705, 911
MspR9I CCNGG 6 cut(s) 249, 296, 371, 705, 911, 969
MunI CAATTG 2 cut(s) 402, 850
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 3 cut(s) 249, 371, 969
MwoI GCNNNNNNNGC 2 cut(s) 43, 781
NciI CCSGG 3 cut(s) 296, 705, 911
NdeI CATATG 1 cut(s) 571
NdeII GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
NlaIII CATG 4 cut(s) 344, 589, 788, 866
NlaIV GGNNCC 3 cut(s) 375, 682, 709
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 388, 652
NsiI ATGCAT 1 cut(s) 329
NspI RCATGY 2 cut(s) 788, 866
NspV TTCGAA 1 cut(s) 822
PciI ACATGT 1 cut(s) 862
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 995, 1008
PkrI GCNGC 4 cut(s) 313, 500, 503, 1055
PscI ACATGT 1 cut(s) 862
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 247, 369, 967
PspEI GGTNACC 1 cut(s) 740
PspGI CCWGG 3 cut(s) 247, 369, 967
PspN4I GGNNCC 3 cut(s) 375, 682, 709
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 223, 283
RsaI GTAC 3 cut(s) 467, 848, 879
RsaNI GTAC 3 cut(s) 466, 847, 878
RseI CAYNNNNRTG 3 cut(s) 332, 789, 831
SacI GAGCTC 1 cut(s) 415
SaqAI TTAA 6 cut(s) 437, 555, 602, 768, 941, 951
SatI GCNGC 4 cut(s) 312, 499, 502, 1054
Sau3AI GATC 7 cut(s) 89, 160, 164, 197, 223, 283, 398
Sau96I GGNCC 1 cut(s) 680
ScrFI CCNGG 6 cut(s) 249, 296, 371, 705, 911, 969
SduI GDGCHC 2 cut(s) 415, 724
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 5 cut(s) 314, 336, 488, 1020, 1030
SfcI CTRYAG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 822
SmiMI CAYNNNNRTG 3 cut(s) 332, 789, 831
Sse9I AATT 5 cut(s) 270, 402, 623, 850, 1044
SsiI CCGC 4 cut(s) 12, 281, 483, 501
SspMI CTAG 2 cut(s) 9, 474
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 6 cut(s) 247, 294, 369, 703, 909, 967
TaaI ACNGT 4 cut(s) 193, 451, 658, 719
TaiI ACGT 1 cut(s) 546
TaqI TCGA 6 cut(s) 17, 163, 357, 789, 822, 1038
TasI AATT 5 cut(s) 270, 402, 623, 850, 1044
TatI WGTACW 2 cut(s) 465, 877
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 995, 1008
Tru1I TTAA 6 cut(s) 437, 555, 602, 768, 941, 951
Tru9I TTAA 6 cut(s) 437, 555, 602, 768, 941, 951
TscAI CASTG 3 cut(s) 456, 724, 729
TseFI GTSAC 2 cut(s) 388, 652
TseI GCWGC 3 cut(s) 311, 498, 1053
Tsp45I GTSAC 2 cut(s) 388, 652
TspDTI ATGAA 5 cut(s) 17, 540, 558, 676, 752
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 3 cut(s) 456, 724, 729
VneI GTGCAC 1 cut(s) 720
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 2 cut(s) 270, 623
XceI RCATGY 2 cut(s) 788, 866
XmiI GTMKAC 1 cut(s) 244
XspI CTAG 2 cut(s) 9, 474
Zsp2I ATGCAT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.