Rw0G018770

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00847
OG0000382
Physical Location & Seq
Reverse (-)
117813 .. 120093
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018770.1

Sequence Viewer

Length: 939 bp
ATGAAGCTTCTGCAAAATGGTTACACTGTTCGAACTACTGTTAGACCTGATCCAGAATGCAAGAGAGACATCAGCTACCTCACAAGCCTGCCTAGAGCATCGGAGAAGCTTCACATCTTCAATGGGGATCTGAACCAACCTGAAAGTTTTAATGTAGCAATTGAAGGATGCACCGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAACTTGATGAAGCATCGGTAACCAAACAATCCGTAGAAGGTACCCTAGGCATATTGAAAGCTTGCCTAAATGCCAAGACTGTGAAAAAGTTTGTTTACACTTCTAGTGCAGCAACTGTTGCTTATAGTGGCAACAACAAAGACATGGCGGATGAGAGTTCATGGAGTGATATAGAGTACCATAGGTCTCTTGGATTATTTAGGAGTTCATCGTATGTGGCTGCTAAGACCAAAACAGAGCAAGCAGTACTGGAATTTGCAGAAAAAAATGGATTAGAACTTGTAACTTTGATCCCTCCATTCGTCCTTGGTGGTTTCATCTGCAAAGATCTCCCTGGTTCAGTGGAATTGATCTTATCCATGATATTAGGAAAAGCAGATAACTTTACACAACTTCACAAGCTAAGTTTGGTACATGTAGATGATTTGGTTAGTGCGCATATCTTTCTTTTTGAAAATTCTAATGCCAAAGGGAGGTACATTTGCTCTTCAACTCCAACATCAATAGACGAAATGTACCGATTTCTTTATGCAAAATACCCTGAATTTCGCATACCCACAACTCAATCACTGAAAGAAGCTTACAAAATTTGTGGTTTCTCATCGAAGAAGCTCCTGAATACTGGATTCACATTTAAGCATGGCCTTGATGACATATTGACTGGAGCATTTCAATCTTGCAGAGAAAAGGGTTTTCTAGAAAGGTTCTTTGGCAAAATGGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

312

Amino Acids

34.77

Weight (kDa)

7.6

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 3 - 226 2.6e-15 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 32 - 185 7.8e-08 Male sterility protein
3Beta_HSD PF01073 36 - 219 6.4e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 648
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 251
AciI CCGC 1 cut(s) 359
AclWI GGATC 3 cut(s) 44, 135, 496
AcsI RAATTY 4 cut(s) 464, 667, 755, 798
AfaI GTAC 6 cut(s) 253, 389, 459, 624, 689, 728
AfiI CCNNNNNNNGG 1 cut(s) 684
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 6 cut(s) 121, 164, 268, 665, 702, 884
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 903, 935
AluBI AGCT 7 cut(s) 7, 75, 109, 272, 613, 791, 823
AluI AGCT 7 cut(s) 7, 75, 109, 272, 613, 791, 823
Alw26I GTCTC 2 cut(s) 60, 402
AlwI GGATC 3 cut(s) 44, 135, 496
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 1 cut(s) 853
ApeKI GCWGC 2 cut(s) 320, 431
ApoI RAATTY 4 cut(s) 464, 667, 755, 798
Asp718I GGTACC 1 cut(s) 251
AspA2I CCTAGG 1 cut(s) 256
AspLEI GCGC 1 cut(s) 649
AsuC2I CCSGG 1 cut(s) 175
AsuII TTCGAA 1 cut(s) 31
AvrII CCTAGG 1 cut(s) 256
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 172
BbvI GCAGC 2 cut(s) 332, 418
BciT130I CCWGG 1 cut(s) 546
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 60, 402
BfaI CTAG 4 cut(s) 93, 257, 315, 908
BglII AGATCT 1 cut(s) 538
BisI GCNGC 2 cut(s) 321, 432
BlnI CCTAGG 1 cut(s) 256
BlsI GCNGC 2 cut(s) 322, 433
BmcAI AGTACT 1 cut(s) 459
Bme1390I CCNGG 2 cut(s) 175, 546
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 175, 546
BmsI GCATC 3 cut(s) 107, 158, 233
BpiI GAAGAC 1 cut(s) 172
BpmI CTGGAG 1 cut(s) 894
Bpu14I TTCGAA 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 4 cut(s) 174, 256, 517, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 684
Bse1I ACTGG 3 cut(s) 465, 838, 877
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 4 cut(s) 174, 256, 517, 544
BseGI GGATG 3 cut(s) 173, 193, 367
BseLI CCNNNNNNNGG 1 cut(s) 684
BseNI ACTGG 3 cut(s) 465, 838, 877
BseXI GCAGC 2 cut(s) 332, 418
BsgI GTGCAG 1 cut(s) 339
BshFI GGCC 1 cut(s) 855
BshNI GGYRCC 1 cut(s) 251
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 684
BsmAI GTCTC 2 cut(s) 60, 402
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 855
Bso31I GGTCTC 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 31
Bsp143I GATC 5 cut(s) 49, 127, 501, 538, 561
BspACI CCGC 1 cut(s) 359
BspANI GGCC 1 cut(s) 855
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 3 cut(s) 44, 135, 496
BspQI GCTCTTC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 402
BsrI ACTGG 3 cut(s) 465, 838, 877
BssECI CCNNGG 4 cut(s) 174, 256, 517, 544
BssMI GATC 5 cut(s) 49, 127, 501, 538, 561
BssT1I CCWWGG 2 cut(s) 256, 517
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 4 cut(s) 28, 40, 292, 328
Bst6I CTCTTC 1 cut(s) 703
BstAPI GCANNNNNTGC 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 89, 274, 453
BstDEI CTNAG 2 cut(s) 435, 614
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 3 cut(s) 173, 193, 367
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 5 cut(s) 52, 130, 504, 541, 564
BstMAI GTCTC 2 cut(s) 60, 402
BstMBI GATC 5 cut(s) 49, 127, 501, 538, 561
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 629
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 173, 544
BstV1I GCAGC 2 cut(s) 332, 418
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 2 cut(s) 127, 538
BstYI RGATCY 2 cut(s) 127, 538
BsuRI GGCC 1 cut(s) 855
BtsCI GGATG 3 cut(s) 173, 193, 367
BtsIMutI CAGTG 3 cut(s) 24, 558, 779
Cac8I GCNNGC 3 cut(s) 89, 274, 453
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 1 cut(s) 649
Csp6I GTAC 6 cut(s) 252, 388, 458, 623, 688, 727
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 7 cut(s) 185, 355, 372, 571, 626, 851, 936
CviQI GTAC 6 cut(s) 252, 388, 458, 623, 688, 727
DdeI CTNAG 2 cut(s) 435, 614
DpnI GATC 5 cut(s) 51, 129, 503, 540, 563
DpnII GATC 5 cut(s) 49, 127, 501, 538, 561
Eam1104I CTCTTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 703
EciI GGCGGA 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 256, 517
Eco31I GGTCTC 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 544
EcoT14I CCWWGG 2 cut(s) 256, 517
ErhI CCWWGG 2 cut(s) 256, 517
FaeI CATG 7 cut(s) 188, 358, 375, 574, 629, 854, 939
FatI CATG 7 cut(s) 184, 354, 371, 570, 625, 850, 935
Fnu4HI GCNGC 2 cut(s) 321, 432
FokI GGATG 3 cut(s) 180, 180, 374
Fsp4HI GCNGC 2 cut(s) 321, 432
FspAI RTGCGCAY 1 cut(s) 648
FspBI CTAG 4 cut(s) 93, 257, 315, 908
FspI TGCGCA 1 cut(s) 648
GlaI GCGC 1 cut(s) 648
GluI GCNGC 2 cut(s) 321, 432
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 1 cut(s) 855
HapII CCGG 1 cut(s) 174
HhaI GCGC 1 cut(s) 649
Hin1II CATG 7 cut(s) 188, 358, 375, 574, 629, 854, 939
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HindIII AAGCTT 4 cut(s) 5, 107, 270, 789
HinfI GANTC 1 cut(s) 837
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 103, 132
Hpy188III TCNNGA 3 cut(s) 53, 826, 908
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 158, 242
HpyCH4III ACNGT 4 cut(s) 28, 40, 292, 328
HpyCH4V TGCA 8 cut(s) 13, 60, 171, 320, 470, 534, 743, 891
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 2 cut(s) 435, 614
Hsp92II CATG 7 cut(s) 188, 358, 375, 574, 629, 854, 939
HspAI GCGC 1 cut(s) 647
KpnI GGTACC 1 cut(s) 255
Kzo9I GATC 5 cut(s) 49, 127, 501, 538, 561
LguI GCTCTTC 1 cut(s) 703
LmnI GCTCC 2 cut(s) 828, 875
Lsp1109I GCAGC 2 cut(s) 332, 418
LweI GCATC 3 cut(s) 107, 158, 233
MaeI CTAG 4 cut(s) 93, 257, 315, 908
MaeIII GTNAC 3 cut(s) 20, 229, 493
MalI GATC 5 cut(s) 51, 129, 503, 540, 563
MboI GATC 5 cut(s) 49, 127, 501, 538, 561
MboII GAAGA 4 cut(s) 109, 172, 690, 829
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 2 cut(s) 127, 538
MluCI AATT 6 cut(s) 159, 464, 557, 667, 755, 798
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 3 cut(s) 89, 516, 678
MseI TTAA 2 cut(s) 150, 846
MslI CAYNNNNRTG 1 cut(s) 630
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 175, 546
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 175
NdeII GATC 5 cut(s) 49, 127, 501, 538, 561
NlaIII CATG 7 cut(s) 188, 358, 375, 574, 629, 854, 939
NlaIV GGNNCC 1 cut(s) 253
NsbI TGCGCA 1 cut(s) 648
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 31
PciI ACATGT 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 703
PctI GAATGC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 837
PkrI GCNGC 2 cut(s) 322, 433
PscI ACATGT 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 544
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 127, 538
RsaI GTAC 6 cut(s) 253, 389, 459, 624, 689, 728
RsaNI GTAC 6 cut(s) 252, 388, 458, 623, 688, 727
RseI CAYNNNNRTG 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 703
SaqAI TTAA 2 cut(s) 150, 846
SatI GCNGC 2 cut(s) 321, 432
Sau3AI GATC 5 cut(s) 49, 127, 501, 538, 561
ScaI AGTACT 1 cut(s) 459
ScrFI CCNGG 2 cut(s) 175, 546
SfaNI GCATC 3 cut(s) 107, 158, 233
SfuI TTCGAA 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 630
Sse9I AATT 6 cut(s) 159, 464, 557, 667, 755, 798
SsiI CCGC 1 cut(s) 359
SspMI CTAG 4 cut(s) 93, 257, 315, 908
StyD4I CCNGG 2 cut(s) 173, 544
StyI CCWWGG 2 cut(s) 256, 517
TaaI ACNGT 4 cut(s) 28, 40, 292, 328
TaqI TCGA 2 cut(s) 31, 815
TasI AATT 6 cut(s) 159, 464, 557, 667, 755, 798
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 2 cut(s) 150, 846
Tru9I TTAA 2 cut(s) 150, 846
TscAI CASTG 3 cut(s) 31, 558, 786
TseI GCWGC 2 cut(s) 320, 431
TspDTI ATGAA 5 cut(s) 17, 234, 360, 408, 517
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 3 cut(s) 31, 558, 786
XapI RAATTY 4 cut(s) 464, 667, 755, 798
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmaJI CCTAGG 1 cut(s) 256
XspI CTAG 4 cut(s) 93, 257, 315, 908
ZrmI AGTACT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.