Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0000382

Physical Location & Seq

Genomic Coordinates Forward (+)

43215499 .. 43217455

Gene Structure 1957 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG222300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGAGCTTGTATATGTTCCATTTGTTTATGGCAGAAGGCAAGAGAGATATCAGCTACCTGACAAGCCTTCCAGGAGCACAAGAGAAACTCCAAATCTTCCATGCAGATCTCAACAACCCCGAGACCTTCAACGACGCCATCCAAGGCTGCACCGGTGTCTTCCACCTTGCTCACCCCATGGACGTTGAAGGCAAAGAACCCGAGGAAACCGTAACCAAACGAGCCCTGGAAGGAACACTAGGCATTTTGAAGGCATGCCTAAGTTGCAAGACGGTGAGGCGAGTGGTTTACACATCCAGTTTGGCCACAATATTGTACAACAGCAAGCGTCTTGAAGAAACTGATGAAAACACATGGAGTGAGGTTGATGTATGCAGAAGTAACAAGCTTGTGAGCTCTTCTTACTTGGTTTCCAAGACTCTGGTGGAGAGGACAGCCCTGGAATTTGCAGAGAGGAATGGTTGGGATCTTGTCACTGTGGTTTTGCCTATAGTAGTTGGACCCTTCGTTTGTCCAAATGTTCCTGCTTCCGTGTACATGGGCATGGCTCCCATTTTTGGTGACCAAGAGCAATGTAAATATCTTGTTAGCACATATATGGTGCACATAGATGATGTGGCGAGTGCCCATATCTTCCTTCTTGAACATGCTAATGCAAAAGGGAGGTACATTTGTTCGTCGATCCAAACAACGATTCATGAGTTATATGACTTTCTTTCCGCAAGATACCCAGAACTTCATATATCAATACCAGATCAGTTAATGGAGATTAAAGATTACAAAGGAACTAATGTATTTTCAATGAAACTGTTGAATACTGGCTTCAAATTTAAATACTGCCTTGGGGAAATGTTTGATGGCGCTATTCAATCTTGCAAAGAGAAGGGTTTTCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009889 GO:0010422 GO:0010817 GO:0016128 GO:0016131 GO:0019216 GO:0019218 GO:0019222 GO:0031323 GO:0031326 GO:0032350 GO:0042445 GO:0044238 GO:0044424 GO:0044464 GO:0046885 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090030 GO:1901360 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Epimerase

Protein Analysis

298

Amino Acids

33.55

Weight (kDa)

5.62

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	9 - 220	2.9e-15	NAD dependent epimerase/dehydratase family
NAD_binding_4	PF07993	28 - 167	3.7e-07	Male sterility protein
3Beta_HSD	PF01073	32 - 162	9.2e-14	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca	FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica	MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica	Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis	pycom08g20120
rosa_chinensis	RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata	RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora	Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii	Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa	Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha	Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana	Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	720
AclWI	GGATC	2 cut(s)	474, 676
AcoI	YGGCCR	1 cut(s)	303
AcsI	RAATTY	2 cut(s)	443, 827
AcyI	GRCGYC	1 cut(s)	135
AfaI	GTAC	3 cut(s)	317, 536, 668
AfiI	CCNNNNNNNGG	1 cut(s)	557
AgeI	ACCGGT	1 cut(s)	152
AgsI	TTSAA	9 cut(s)	130, 188, 250, 335, 644, 801, 814, 826, 869
AjnI	CCWGG	3 cut(s)	70, 225, 438
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
AluBI	AGCT	4 cut(s)	6, 54, 388, 396
AluI	AGCT	4 cut(s)	6, 54, 388, 396
Alw21I	GWGCWC	3 cut(s)	79, 398, 606
Alw26I	GTCTC	1 cut(s)	116
Alw44I	GTGCAC	1 cut(s)	602
AlwI	GGATC	2 cut(s)	474, 676
Ama87I	CYCGRG	2 cut(s)	119, 200
AoxI	GGCC	1 cut(s)	303
ApaLI	GTGCAC	1 cut(s)	602
ApeKI	GCWGC	1 cut(s)	147
ApoI	RAATTY	2 cut(s)	443, 827
ArsI	GACNNNNNNTTYG	2 cut(s)	492, 524
AsiGI	ACCGGT	1 cut(s)	152
AspLEI	GCGC	1 cut(s)	863
AspS9I	GGNCC	1 cut(s)	500
AsuHPI	GGTGA	3 cut(s)	164, 286, 572
AvaI	CYCGRG	2 cut(s)	119, 200
AvaII	GGWCC	1 cut(s)	500
BaeGI	GKGCMC	2 cut(s)	606, 628
BaeI	ACNNNNGTAYC	2 cut(s)	658, 691
BalI	TGGCCA	1 cut(s)	305
BanII	GRGCYC	2 cut(s)	226, 398
BbsI	GAAGAC	1 cut(s)	151
Bbv12I	GWGCWC	3 cut(s)	79, 398, 606
BbvI	GCAGC	1 cut(s)	134
BccI	CCATC	2 cut(s)	146, 851
BciT130I	CCWGG	3 cut(s)	72, 227, 440
BcoDI	GTCTC	1 cut(s)	116
BfaI	CTAG	1 cut(s)	239
BfmI	CTRYAG	1 cut(s)	489
BfoI	RGCGCY	1 cut(s)	864
BglII	AGATCT	1 cut(s)	106
BisI	GCNGC	1 cut(s)	148
BlsI	GCNGC	1 cut(s)	149
Bme1390I	CCNGG	3 cut(s)	72, 227, 440
Bme18I	GGWCC	1 cut(s)	500
BmeT110I	CYCGRG	2 cut(s)	119, 200
BmgT120I	GGNCC	1 cut(s)	500
BmiI	GGNNCC	2 cut(s)	502, 549
BmrFI	CCNGG	3 cut(s)	72, 227, 440
BpiI	GAAGAC	1 cut(s)	151
BsaHI	GRCGYC	1 cut(s)	135
BsaI	GGTCTC	1 cut(s)	116
BsaJI	CCNNGG	6 cut(s)	142, 177, 201, 225, 438, 841
BsaWI	WCCGGW	1 cut(s)	152
Bsc4I	CCNNNNNNNGG	1 cut(s)	557
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	2 cut(s)	297, 823
Bse3DI	GCAATG	1 cut(s)	578
BseBI	CCWGG	3 cut(s)	72, 227, 440
BseDI	CCNNGG	6 cut(s)	142, 177, 201, 225, 438, 841
BseGI	GGATG	2 cut(s)	138, 293
BseLI	CCNNNNNNNGG	1 cut(s)	557
BseMI	GCAATG	1 cut(s)	578
BseNI	ACTGG	2 cut(s)	297, 823
BseSI	GKGCMC	2 cut(s)	606, 628
BseXI	GCAGC	1 cut(s)	134
BsgI	GTGCAG	1 cut(s)	133
BshFI	GGCC	1 cut(s)	305
BshTI	ACCGGT	1 cut(s)	152
BsiHKAI	GWGCWC	3 cut(s)	79, 398, 606
BsiHKCI	CYCGRG	2 cut(s)	119, 200
BsiSI	CCGG	1 cut(s)	153
BslI	CCNNNNNNNGG	1 cut(s)	557
BsmAI	GTCTC	1 cut(s)	116
BsnI	GGCC	1 cut(s)	305
Bso31I	GGTCTC	1 cut(s)	116
BsoBI	CYCGRG	2 cut(s)	119, 200
Bsp1286I	GDGCHC	5 cut(s)	79, 226, 398, 606, 628
Bsp1407I	TGTACA	2 cut(s)	315, 534
Bsp143I	GATC	4 cut(s)	106, 466, 681, 754
Bsp19I	CCATGG	1 cut(s)	177
BspACI	CCGC	1 cut(s)	720
BspANI	GGCC	1 cut(s)	305
BspHI	TCATGA	1 cut(s)	697
BspLI	GGNNCC	2 cut(s)	502, 549
BspPI	GGATC	2 cut(s)	474, 676
BspQI	GCTCTTC	1 cut(s)	403
BspTNI	GGTCTC	1 cut(s)	116
BsrDI	GCAATG	1 cut(s)	578
BsrFI	RCCGGY	1 cut(s)	152
BsrGI	TGTACA	2 cut(s)	315, 534
BsrI	ACTGG	2 cut(s)	297, 823
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	6 cut(s)	142, 177, 201, 225, 438, 841
BssMI	GATC	4 cut(s)	106, 466, 681, 754
BssNI	GRCGYC	1 cut(s)	135
BssT1I	CCWWGG	3 cut(s)	142, 177, 841
Bst2UI	CCWGG	3 cut(s)	72, 227, 440
Bst4CI	ACNGT	4 cut(s)	211, 274, 478, 810
Bst6I	CTCTTC	1 cut(s)	403
BstACI	GRCGYC	1 cut(s)	135
BstAUI	TGTACA	2 cut(s)	315, 534
BstC8I	GCNNGC	2 cut(s)	256, 326
BstDEI	CTNAG	1 cut(s)	260
BstDSI	CCRYGG	1 cut(s)	177
BstEII	GGTNACC	1 cut(s)	560
BstF5I	GGATG	2 cut(s)	138, 293
BstH2I	RGCGCY	1 cut(s)	864
BstHHI	GCGC	1 cut(s)	863
BstKTI	GATC	4 cut(s)	109, 469, 684, 757
BstMAI	GTCTC	1 cut(s)	116
BstMBI	GATC	4 cut(s)	106, 466, 681, 754
BstMWI	GCNNNNNNNGC	1 cut(s)	264
BstNI	CCWGG	3 cut(s)	72, 227, 440
BstNSI	RCATGY	2 cut(s)	258, 650
BstPI	GGTNACC	1 cut(s)	560
BstSCI	CCNGG	3 cut(s)	70, 225, 438
BstSFI	CTRYAG	1 cut(s)	489
BstSLI	GKGCMC	2 cut(s)	606, 628
BstV1I	GCAGC	1 cut(s)	134
BstV2I	GAAGAC	1 cut(s)	151
BstX2I	RGATCY	2 cut(s)	106, 466
BstXI	CCANNNNNNTGG	1 cut(s)	421
BstYI	RGATCY	2 cut(s)	106, 466
BsuRI	GGCC	1 cut(s)	305
BtgI	CCRYGG	1 cut(s)	177
BtsCI	GGATG	2 cut(s)	138, 293
BtsIMutI	CAGTG	1 cut(s)	474
Cac8I	GCNNGC	2 cut(s)	256, 326
CciI	TCATGA	1 cut(s)	697
CfoI	GCGC	1 cut(s)	863
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	1 cut(s)	500
CseI	GACGC	2 cut(s)	143, 317
Csp6I	GTAC	3 cut(s)	316, 535, 667
CspAI	ACCGGT	1 cut(s)	152
CviAII	CATG	8 cut(s)	101, 178, 255, 354, 538, 544, 647, 698
CviQI	GTAC	3 cut(s)	316, 535, 667
DdeI	CTNAG	1 cut(s)	260
DpnI	GATC	4 cut(s)	108, 468, 683, 756
DpnII	GATC	4 cut(s)	106, 466, 681, 754
DraI	TTTAAA	1 cut(s)	832
EaeI	YGGCCR	1 cut(s)	303
Eam1104I	CTCTTC	1 cut(s)	403
EarI	CTCTTC	1 cut(s)	403
Ecl136II	GAGCTC	1 cut(s)	396
Eco130I	CCWWGG	3 cut(s)	142, 177, 841
Eco24I	GRGCYC	2 cut(s)	226, 398
Eco31I	GGTCTC	1 cut(s)	116
Eco32I	GATATC	1 cut(s)	49
Eco47I	GGWCC	1 cut(s)	500
Eco53kI	GAGCTC	1 cut(s)	396
Eco88I	CYCGRG	2 cut(s)	119, 200
Eco91I	GGTNACC	1 cut(s)	560
EcoICRI	GAGCTC	1 cut(s)	396
EcoO65I	GGTNACC	1 cut(s)	560
EcoRII	CCWGG	3 cut(s)	70, 225, 438
EcoRV	GATATC	1 cut(s)	49
EcoT14I	CCWWGG	3 cut(s)	142, 177, 841
EcoT38I	GRGCYC	2 cut(s)	226, 398
ErhI	CCWWGG	3 cut(s)	142, 177, 841
FaeI	CATG	8 cut(s)	104, 181, 258, 357, 541, 547, 650, 701
FatI	CATG	8 cut(s)	100, 177, 254, 353, 537, 543, 646, 697
Fnu4HI	GCNGC	1 cut(s)	148
FokI	GGATG	2 cut(s)	125, 280
FriOI	GRGCYC	2 cut(s)	226, 398
Fsp4HI	GCNGC	1 cut(s)	148
FspBI	CTAG	1 cut(s)	239
GlaI	GCGC	1 cut(s)	862
GluI	GCNGC	1 cut(s)	148
HaeII	RGCGCY	1 cut(s)	864
HaeIII	GGCC	1 cut(s)	305
HapII	CCGG	1 cut(s)	153
HgaI	GACGC	2 cut(s)	143, 317
HhaI	GCGC	1 cut(s)	863
Hin1I	GRCGYC	1 cut(s)	135
Hin1II	CATG	8 cut(s)	104, 181, 258, 357, 541, 547, 650, 701
Hin6I	GCGC	1 cut(s)	861
HinP1I	GCGC	1 cut(s)	861
HindIII	AAGCTT	1 cut(s)	386
HinfI	GANTC	2 cut(s)	418, 694
HpaII	CCGG	1 cut(s)	153
HphI	GGTGA	3 cut(s)	164, 286, 572
Hpy166II	GTNNAC	3 cut(s)	289, 535, 604
Hpy188III	TCNNGA	3 cut(s)	332, 641, 698
Hpy8I	GTNNAC	3 cut(s)	289, 535, 604
Hpy99I	CGWCG	2 cut(s)	137, 682
HpyAV	CCTTC	9 cut(s)	29, 77, 136, 182, 224, 244, 514, 647, 877
HpyCH4III	ACNGT	4 cut(s)	211, 274, 478, 810
HpyCH4IV	ACGT	1 cut(s)	183
HpyCH4V	TGCA	8 cut(s)	104, 150, 267, 375, 449, 604, 656, 876
HpyF10VI	GCNNNNNNNGC	1 cut(s)	264
HpyF3I	CTNAG	1 cut(s)	260
HpySE526I	ACGT	1 cut(s)	183
Hsp92I	GRCGYC	1 cut(s)	135
Hsp92II	CATG	8 cut(s)	104, 181, 258, 357, 541, 547, 650, 701
HspAI	GCGC	1 cut(s)	861
Kzo9I	GATC	4 cut(s)	106, 466, 681, 754
LguI	GCTCTTC	1 cut(s)	403
LmnI	GCTCC	2 cut(s)	74, 553
Lsp1109I	GCAGC	1 cut(s)	134
MaeI	CTAG	1 cut(s)	239
MaeII	ACGT	1 cut(s)	183
MaeIII	GTNAC	4 cut(s)	211, 380, 472, 560
MalI	GATC	4 cut(s)	108, 468, 683, 756
MboI	GATC	4 cut(s)	106, 466, 681, 754
MboII	GAAGA	5 cut(s)	88, 151, 347, 390, 625
MflI	RGATCY	2 cut(s)	106, 466
MhlI	GDGCHC	5 cut(s)	79, 226, 398, 606, 628
MlsI	TGGCCA	1 cut(s)	305
MluCI	AATT	2 cut(s)	443, 827
MluNI	TGGCCA	1 cut(s)	305
MlyI	GAGTC	1 cut(s)	412
MmeI	TCCRAC	1 cut(s)	478
MnlI	CCTC	6 cut(s)	196, 270, 355, 423, 447, 657
Mox20I	TGGCCA	1 cut(s)	305
MscI	TGGCCA	1 cut(s)	305
MseI	TTAA	3 cut(s)	761, 771, 831
MslI	CAYNNNNRTG	4 cut(s)	542, 596, 609, 651
Msp20I	TGGCCA	1 cut(s)	305
MspI	CCGG	1 cut(s)	153
MspR9I	CCNGG	3 cut(s)	72, 227, 440
MvaI	CCWGG	3 cut(s)	72, 227, 440
MwoI	GCNNNNNNNGC	1 cut(s)	264
NcoI	CCATGG	1 cut(s)	177
NdeII	GATC	4 cut(s)	106, 466, 681, 754
NlaIII	CATG	8 cut(s)	104, 181, 258, 357, 541, 547, 650, 701
NlaIV	GGNNCC	2 cut(s)	502, 549
NmuCI	GTSAC	2 cut(s)	472, 560
NspI	RCATGY	2 cut(s)	258, 650
PaeI	GCATGC	1 cut(s)	258
PagI	TCATGA	1 cut(s)	697
PciSI	GCTCTTC	1 cut(s)	403
PfeI	GAWTC	1 cut(s)	694
PfoI	TCCNGGA	1 cut(s)	70
PinAI	ACCGGT	1 cut(s)	152
PkrI	GCNGC	1 cut(s)	149
PleI	GAGTC	1 cut(s)	412
PpsI	GAGTC	1 cut(s)	412
Psp124BI	GAGCTC	1 cut(s)	398
Psp6I	CCWGG	3 cut(s)	70, 225, 438
PspEI	GGTNACC	1 cut(s)	560
PspGI	CCWGG	3 cut(s)	70, 225, 438
PspN4I	GGNNCC	2 cut(s)	502, 549
PspPI	GGNCC	1 cut(s)	500
PsuI	RGATCY	2 cut(s)	106, 466
RsaI	GTAC	3 cut(s)	317, 536, 668
RsaNI	GTAC	3 cut(s)	316, 535, 667
RseI	CAYNNNNRTG	4 cut(s)	542, 596, 609, 651
SacI	GAGCTC	1 cut(s)	398
SapI	GCTCTTC	1 cut(s)	403
SaqAI	TTAA	3 cut(s)	761, 771, 831
SatI	GCNGC	1 cut(s)	148
Sau3AI	GATC	4 cut(s)	106, 466, 681, 754
Sau96I	GGNCC	1 cut(s)	500
SchI	GAGTC	1 cut(s)	412
ScrFI	CCNGG	3 cut(s)	72, 227, 440
SduI	GDGCHC	5 cut(s)	79, 226, 398, 606, 628
SfcI	CTRYAG	1 cut(s)	489
SgrAI	CRCCGGYG	1 cut(s)	152
SinI	GGWCC	1 cut(s)	500
SmiI	ATTTAAAT	1 cut(s)	832
SmiMI	CAYNNNNRTG	4 cut(s)	542, 596, 609, 651
SphI	GCATGC	1 cut(s)	258
Sse9I	AATT	2 cut(s)	443, 827
SsiI	CCGC	1 cut(s)	720
SspI	AATATT	1 cut(s)	312
SspMI	CTAG	1 cut(s)	239
SstI	GAGCTC	1 cut(s)	398
StyD4I	CCNGG	3 cut(s)	70, 225, 438
StyI	CCWWGG	3 cut(s)	142, 177, 841
SwaI	ATTTAAAT	1 cut(s)	832
TaaI	ACNGT	4 cut(s)	211, 274, 478, 810
TaiI	ACGT	1 cut(s)	186
TaqI	TCGA	1 cut(s)	680
TasI	AATT	2 cut(s)	443, 827
TatI	WGTACW	2 cut(s)	315, 534
TfiI	GAWTC	1 cut(s)	694
Tru1I	TTAA	3 cut(s)	761, 771, 831
Tru9I	TTAA	3 cut(s)	761, 771, 831
TscAI	CASTG	1 cut(s)	481
TseFI	GTSAC	2 cut(s)	472, 560
TseI	GCWGC	1 cut(s)	147
Tsp45I	GTSAC	2 cut(s)	472, 560
TspDTI	ATGAA	4 cut(s)	360, 686, 728, 818
TspGWI	ACGGA	1 cut(s)	520
TspRI	CASTG	1 cut(s)	481
VneI	GTGCAC	1 cut(s)	602
VpaK11BI	GGWCC	1 cut(s)	500
XapI	RAATTY	2 cut(s)	443, 827
XceI	RCATGY	2 cut(s)	258, 650
XcmI	CCANNNNNNNNNTGG	2 cut(s)	223, 421
XspI	CTAG	1 cut(s)	239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.