RchiOBHm_Chr1g0350271

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000382
Physical Location & Seq
Forward (+)
43438136 .. 43440732
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57615

Sequence Viewer

Length: 522 bp
ATGTTGGTTGTCTTGGTCTTATGGGCCGCTGTTACTAGTAGGCGATGGCGTTTGGAGCAGTTATGGATTGATGGTGTCGACAATAGGGTGTTGATGGTGTTGGGTTTTCTTAGTCCCAAGTCAGAATGTTCGGCAATCCTTTTGGTCGGATTTAGGTCACAACCAGTGTTGGCTTGGTCGATCAAAAATTGGTCAGGAGGACTCAGACCCAAGCCTGGTAGTCTGGTACATGTAGATGATTTGGTTAGTGTACATATCTTTCTTTTTGAATATCCTGATGCAAAAGGGAGGTACATTTGTTCATCGACTCCATTAACCTTGGAGGAAATGTCCATATTTCTCTCGGCAGAATACCCTGAATTTCAAATACCAACAGCTGATTCTTTGAAGGGCGTTGAAGGTTTCAAAATGTGTCGGTACTCTTCGGAAAAGCAACTAAATTCTGGATTTAAGTTTAAGCATGGGCTCGATGATATGTACGATGGAGCAGTTCACTCTTGCATAGAAAAGGGTTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

173

Amino Acids

19.51

Weight (kDa)

6.42

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 78
AciI CCGC 1 cut(s) 27
AcsI RAATTY 2 cut(s) 359, 439
AfaI GTAC 5 cut(s) 228, 252, 293, 419, 479
AfiI CCNNNNNNNGG 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 5 cut(s) 269, 365, 388, 398, 406
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
AoxI GGCC 1 cut(s) 24
ApoI RAATTY 2 cut(s) 359, 439
AspS9I GGNCC 1 cut(s) 24
BaeI ACNNNNGTAYC 2 cut(s) 283, 316
BanII GRGCYC 1 cut(s) 468
BccI CCATC 4 cut(s) 39, 65, 88, 476
BciT130I CCWGG 1 cut(s) 216
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 318
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 164
BshFI GGCC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 468
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 1 cut(s) 27
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 1 cut(s) 216
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 216
Bst6I CTCTTC 1 cut(s) 427
BstAUI TGTACA 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 110, 203
BstKTI GATC 1 cut(s) 183
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 214
BsuRI GGCC 1 cut(s) 26
BtgZI GCGATG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 24
Csp6I GTAC 5 cut(s) 227, 251, 292, 418, 478
CviAII CATG 2 cut(s) 230, 461
CviJI RGCY 5 cut(s) 26, 173, 214, 377, 466
CviKI_1 RGCY 5 cut(s) 26, 173, 214, 377, 466
CviQI GTAC 5 cut(s) 227, 251, 292, 418, 478
DdeI CTNAG 2 cut(s) 110, 203
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 427
EarI CTCTTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 233, 464
FaiI YATR 8 cut(s) 22, 64, 231, 255, 335, 462, 476, 503
FaqI GGGAC 1 cut(s) 99
FatI CATG 2 cut(s) 229, 460
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 27
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 36
GluI GCNGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 26
Hin1II CATG 2 cut(s) 233, 464
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 3 cut(s) 201, 307, 380
Hpy166II GTNNAC 3 cut(s) 79, 251, 493
Hpy188I TCNGA 4 cut(s) 124, 149, 206, 427
Hpy188III TCNNGA 3 cut(s) 195, 275, 444
Hpy8I GTNNAC 3 cut(s) 79, 251, 493
HpyAV CCTTC 2 cut(s) 382, 392
HpyCH4V TGCA 2 cut(s) 281, 501
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 2 cut(s) 110, 203
Hsp92II CATG 2 cut(s) 233, 464
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 55, 485
LpnPI CCDG 8 cut(s) 177, 180, 201, 209, 228, 288, 369, 429
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 31, 156
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 3 cut(s) 187, 359, 439
MlyI GAGTC 2 cut(s) 195, 301
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 3 cut(s) 191, 282, 316
MseI TTAA 3 cut(s) 314, 450, 456
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 29, 377
MspR9I CCNGG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 1 cut(s) 180
NlaIII CATG 2 cut(s) 233, 464
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 156
NspI RCATGY 1 cut(s) 233
PciI ACATGT 1 cut(s) 229
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 2 cut(s) 195, 301
PpsI GAGTC 2 cut(s) 195, 301
PscI ACATGT 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspPI GGNCC 1 cut(s) 24
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 5 cut(s) 228, 252, 293, 419, 479
RsaNI GTAC 5 cut(s) 227, 251, 292, 418, 478
RseI CAYNNNNRTG 1 cut(s) 234
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 3 cut(s) 314, 450, 456
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 2 cut(s) 195, 301
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 468
SetI ASST 5 cut(s) 158, 293, 320, 379, 403
SfaNI GCATC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 234
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 3 cut(s) 187, 359, 439
SsiI CCGC 1 cut(s) 27
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 318
TaqI TCGA 4 cut(s) 78, 179, 305, 468
TasI AATT 3 cut(s) 187, 359, 439
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 3 cut(s) 314, 450, 456
Tru9I TTAA 3 cut(s) 314, 450, 456
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 1 cut(s) 291
TspRI CASTG 1 cut(s) 171
XapI RAATTY 2 cut(s) 359, 439
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.