MD08G1231400.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000382
Physical Location & Seq
Reverse (-)
29753166 .. 29760528
7363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1231400.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGAAGGAGAGAGATCAGGAAGAGTTGTATGTGTAACAGGTGGTTCAGGGTTCGTAGGGTCTTGGCTGGTTATGAGGCTTCTTGATCAAGGCTACTCTGTGAATACCACTGTTCGATCACACCCTGCAGGGAACCAGAGAGATCTAAGCTACCTCACAAGTCTACCAGGAGCAACCGAAAGGCTCAAAATTTTCACAGCGGATCTCAGCAACCCGGACAGCTTCAATGCAGCCGTTGAAGGATGCATTGGAGTGTTCCATGTTGCCACTCCAATCGATTTCCAAGACAAGGAACCAGAACAAGTAGTGACCAAAAGATCAATTGATGGAGCTCTCGGCATTCTAAAGGCGTGCTTAAATGCGAAAACAGTGAAACGAGTTGTGTACACTTCTAGTGCATCCGCTGTCATGTTTGGCAGCGGTAATGATGTGGAGGAAGTGGATGAAAGTTTTTGGAGTGACATAGATTACATTAAAGCTAAAATTTCATATGGAGGGTCTTACATGGTTTCAAAGACATTAACTGAGAAGGCAGTTTTTGAATTTTCAGAGAAATATGGGTTGGATGTTGTGACAGTAATGCCTTCTTTTGTTGTTGGCCCCTTTATTTGCCCTAAGCTTCCCGGATCCGTTCACAGCACACTGGCTATGGTCTTTGGTAACCTTGATGGGCTTAGCTTTCTTATCAATACGCCTATGGTGCATGTCGATGACGTTGCCAGAGCGCACATTTTCCTTTTCGAACATCATGGTGCAAAAGGGAGGTACAATTGCTCATCACATGTCATAACCCTTGTACAAATGGCTGAGTTTCTTTCTGCCAAATACCCGGAATTTCAAATACCGTCACTAAGCTATTTAAGGGAGGTTAAAGGTGAGAAAATGCCTGGTCTGTCATCGAAAAAGCTCTTGGATTCTGGTTTCAGATTCAAGTATGGGGTTGATGAGATTCTCGGCGATGCAATTCAATGCTGCAAAGAAAAGCATTATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

332

Amino Acids

36.28

Weight (kDa)

6.15

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 135 1.3e-06 NmrA-like family
Epimerase PF01370 10 - 258 4.6e-27 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 12 - 244 1.9e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 12 - 201 6.4e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 12 - 213 3e-13 Male sterility protein
NAD_binding_10 PF13460 14 - 136 5.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 163
AciI CCGC 3 cut(s) 200, 402, 420
AclWI GGATC 3 cut(s) 210, 621, 634
AcsI RAATTY 4 cut(s) 189, 483, 542, 833
AfaI GTAC 3 cut(s) 386, 767, 798
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 7 cut(s) 226, 239, 513, 542, 839, 931, 968
AjnI CCWGG 2 cut(s) 166, 886
AjuI GAANNNNNNNTTGG 6 cut(s) 231, 263, 545, 577, 893, 925
AluBI AGCT 8 cut(s) 150, 222, 332, 479, 619, 678, 855, 907
AluI AGCT 8 cut(s) 150, 222, 332, 479, 619, 678, 855, 907
Alw21I GWGCWC 1 cut(s) 334
AlwI GGATC 3 cut(s) 210, 621, 634
AoxI GGCC 1 cut(s) 598
ApeKI GCWGC 3 cut(s) 230, 417, 972
ApoI RAATTY 4 cut(s) 189, 483, 542, 833
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 3 cut(s) 215, 624, 830
AsuHPI GGTGA 1 cut(s) 887
AsuII TTCGAA 1 cut(s) 741
BamHI GGATCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 3 cut(s) 242, 429, 959
BccI CCATC 2 cut(s) 320, 662
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 2 cut(s) 168, 888
BclI TGATCA 1 cut(s) 85
BcnI CCSGG 3 cut(s) 215, 624, 830
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 126
BglII AGATCT 1 cut(s) 142
BisI GCNGC 3 cut(s) 231, 418, 973
BlpI GCTNAGC 1 cut(s) 674
BlsI GCNGC 3 cut(s) 232, 419, 974
Bme1390I CCNGG 5 cut(s) 168, 215, 624, 830, 888
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 4 cut(s) 134, 294, 601, 628
BmrFI CCNGG 5 cut(s) 168, 215, 624, 830, 888
BmsI GCATC 3 cut(s) 233, 407, 949
Bpu10I CCTNAGC 1 cut(s) 615
Bpu1102I GCTNAGC 1 cut(s) 674
Bpu14I TTCGAA 1 cut(s) 741
BpuMI CCSGG 3 cut(s) 215, 624, 830
Bsa29I ATCGAT 1 cut(s) 276
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 648
BseBI CCWGG 2 cut(s) 168, 888
BseCI ATCGAT 1 cut(s) 276
BseGI GGATG 4 cut(s) 248, 398, 448, 571
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMII CTCAG 3 cut(s) 220, 516, 798
BseNI ACTGG 1 cut(s) 648
BseXI GCAGC 3 cut(s) 242, 429, 959
BshFI GGCC 1 cut(s) 600
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 3 cut(s) 215, 624, 830
BslI CCNNNNNNNGG 1 cut(s) 289
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 1 cut(s) 600
Bsp119I TTCGAA 1 cut(s) 741
Bsp1286I GDGCHC 1 cut(s) 334
Bsp1407I TGTACA 2 cut(s) 384, 796
Bsp143I GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
Bsp1720I GCTNAGC 1 cut(s) 674
BspACI CCGC 3 cut(s) 200, 402, 420
BspANI GGCC 1 cut(s) 600
BspCNI CTCAG 3 cut(s) 219, 517, 799
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 4 cut(s) 134, 294, 601, 628
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 3 cut(s) 210, 621, 634
BspT104I TTCGAA 1 cut(s) 741
BsrGI TGTACA 2 cut(s) 384, 796
BsrI ACTGG 1 cut(s) 648
BssMI GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
Bst2UI CCWGG 2 cut(s) 168, 888
Bst4CI ACNGT 4 cut(s) 112, 370, 577, 846
Bst6I CTCTTC 1 cut(s) 16
BstAUI TGTACA 2 cut(s) 384, 796
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 7 cut(s) 146, 206, 525, 615, 674, 807, 851
BstEII GGTNACC 1 cut(s) 659
BstF5I GGATG 4 cut(s) 248, 398, 448, 571
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 7 cut(s) 17, 88, 119, 145, 205, 320, 629
BstMBI GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
BstMWI GCNNNNNNNGC 1 cut(s) 700
BstNI CCWGG 2 cut(s) 168, 888
BstNSI RCATGY 2 cut(s) 707, 785
BstPI GGTNACC 1 cut(s) 659
BstSCI CCNGG 5 cut(s) 166, 213, 622, 828, 886
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 3 cut(s) 242, 429, 959
BstX2I RGATCY 3 cut(s) 142, 202, 626
BstYI RGATCY 3 cut(s) 142, 202, 626
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 1 cut(s) 600
BsuTUI ATCGAT 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 972
BtsCI GGATG 4 cut(s) 248, 398, 448, 571
BtsIMutI CAGTG 3 cut(s) 108, 375, 641
Cac8I GCNNGC 1 cut(s) 352
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 1 cut(s) 599
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 3 cut(s) 385, 766, 797
CviAII CATG 6 cut(s) 260, 409, 505, 704, 749, 782
CviQI GTAC 3 cut(s) 385, 766, 797
DdeI CTNAG 7 cut(s) 146, 206, 525, 615, 674, 807, 851
DpnI GATC 7 cut(s) 16, 87, 118, 144, 204, 319, 628
DpnII GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 659
EcoICRI GAGCTC 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 166, 886
EcoT22I ATGCAT 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 334
FaeI CATG 6 cut(s) 263, 412, 508, 707, 752, 785
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FatI CATG 6 cut(s) 259, 408, 504, 703, 748, 781
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 3 cut(s) 231, 418, 973
FokI GGATG 4 cut(s) 255, 385, 455, 578
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 231, 418, 973
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 726
GluI GCNGC 3 cut(s) 231, 418, 973
HaeIII GGCC 1 cut(s) 600
HapII CCGG 3 cut(s) 215, 624, 830
HhaI GCGC 1 cut(s) 727
Hin1II CATG 6 cut(s) 263, 412, 508, 707, 752, 785
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 3 cut(s) 914, 927, 949
HpaII CCGG 3 cut(s) 215, 624, 830
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 4 cut(s) 164, 385, 387, 634
Hpy188I TCNGA 2 cut(s) 550, 926
Hpy188III TCNNGA 2 cut(s) 18, 83
Hpy8I GTNNAC 4 cut(s) 164, 385, 387, 634
HpyAV CCTTC 3 cut(s) 233, 523, 594
HpyCH4III ACNGT 4 cut(s) 112, 370, 577, 846
HpyCH4IV ACGT 1 cut(s) 714
HpyCH4V TGCA 8 cut(s) 128, 230, 246, 398, 703, 755, 962, 975
HpyF10VI GCNNNNNNNGC 1 cut(s) 700
HpyF3I CTNAG 7 cut(s) 146, 206, 525, 615, 674, 807, 851
HpySE526I ACGT 1 cut(s) 714
Hsp92II CATG 6 cut(s) 263, 412, 508, 707, 752, 785
HspAI GCGC 1 cut(s) 725
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
LmnI GCTCC 2 cut(s) 170, 329
Lsp1109I GCAGC 3 cut(s) 242, 429, 959
LweI GCATC 3 cut(s) 233, 407, 949
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 714
MaeIII GTNAC 6 cut(s) 34, 307, 458, 571, 659, 846
MalI GATC 7 cut(s) 16, 87, 118, 144, 204, 319, 628
MboI GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
MboII GAAGA 1 cut(s) 33
MfeI CAATTG 2 cut(s) 321, 769
MflI RGATCY 3 cut(s) 142, 202, 626
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 7 cut(s) 189, 321, 483, 542, 769, 833, 963
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 6 cut(s) 69, 164, 427, 488, 756, 859
Mph1103I ATGCAT 1 cut(s) 248
MseI TTAA 5 cut(s) 356, 474, 521, 860, 870
MslI CAYNNNNRTG 3 cut(s) 251, 708, 750
MspA1I CMGCKG 3 cut(s) 200, 404, 420
MspI CCGG 3 cut(s) 215, 624, 830
MspR9I CCNGG 5 cut(s) 168, 215, 624, 830, 888
MunI CAATTG 2 cut(s) 321, 769
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 2 cut(s) 168, 888
MwoI GCNNNNNNNGC 1 cut(s) 700
NciI CCSGG 3 cut(s) 215, 624, 830
NdeI CATATG 1 cut(s) 490
NdeII GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
NlaIII CATG 6 cut(s) 263, 412, 508, 707, 752, 785
NlaIV GGNNCC 4 cut(s) 134, 294, 601, 628
NmeAIII GCCGAG 2 cut(s) 315, 933
NmuCI GTSAC 4 cut(s) 307, 458, 571, 846
NsiI ATGCAT 1 cut(s) 248
NspI RCATGY 2 cut(s) 707, 785
NspV TTCGAA 1 cut(s) 741
PciI ACATGT 1 cut(s) 781
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 914, 927, 949
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 3 cut(s) 232, 419, 974
PscI ACATGT 1 cut(s) 781
Psp124BI GAGCTC 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 166, 886
PspEI GGTNACC 1 cut(s) 659
PspGI CCWGG 2 cut(s) 166, 886
PspN4I GGNNCC 4 cut(s) 134, 294, 601, 628
PspPI GGNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 130
PsuI RGATCY 3 cut(s) 142, 202, 626
RsaI GTAC 3 cut(s) 386, 767, 798
RsaNI GTAC 3 cut(s) 385, 766, 797
RseI CAYNNNNRTG 3 cut(s) 251, 708, 750
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 5 cut(s) 356, 474, 521, 860, 870
SatI GCNGC 3 cut(s) 231, 418, 973
Sau3AI GATC 7 cut(s) 14, 85, 116, 142, 202, 317, 626
Sau96I GGNCC 1 cut(s) 599
SbfI CCTGCAGG 1 cut(s) 130
ScrFI CCNGG 5 cut(s) 168, 215, 624, 830, 888
SdaI CCTGCAGG 1 cut(s) 130
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 3 cut(s) 233, 407, 949
SfcI CTRYAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 741
SmiMI CAYNNNNRTG 3 cut(s) 251, 708, 750
Sse8387I CCTGCAGG 1 cut(s) 130
Sse9I AATT 7 cut(s) 189, 321, 483, 542, 769, 833, 963
SsiI CCGC 3 cut(s) 200, 402, 420
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 5 cut(s) 166, 213, 622, 828, 886
TaaI ACNGT 4 cut(s) 112, 370, 577, 846
TaiI ACGT 1 cut(s) 717
TaqI TCGA 5 cut(s) 115, 276, 708, 741, 899
TasI AATT 7 cut(s) 189, 321, 483, 542, 769, 833, 963
TatI WGTACW 2 cut(s) 384, 796
TfiI GAWTC 3 cut(s) 914, 927, 949
Tru1I TTAA 5 cut(s) 356, 474, 521, 860, 870
Tru9I TTAA 5 cut(s) 356, 474, 521, 860, 870
TscAI CASTG 3 cut(s) 115, 375, 648
TseFI GTSAC 4 cut(s) 307, 458, 571, 846
TseI GCWGC 3 cut(s) 230, 417, 972
Tsp45I GTSAC 4 cut(s) 307, 458, 571, 846
TspDTI ATGAA 2 cut(s) 459, 477
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 3 cut(s) 115, 375, 648
XapI RAATTY 4 cut(s) 189, 483, 542, 833
XceI RCATGY 2 cut(s) 707, 785
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.