Prupe.1G338100_v2.0.a1

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013510
Physical Location & Seq
Forward (+)
31858409 .. 31864743
6335 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G338100.1

Sequence Viewer

Length: 1803 bp
ATGCTGGCTCTTGGAAGTCCTACCATCAGAGTCCGTACAAGCACCAACTGCAATGTTTTACTCAAAGAGCTTCAGCAAATATGGAATGACATTGGTGAGAGCGAAGCAGACCAAGACCGCATGCTGTTGGAGTTGGAGAGGGAATGCTTAGATGTTTACAGGAGGAAGGTTGATGAAGCTGCCAATGCCAAGGCACGCCTTCATCAGTCTGTTGCAGCCAAAGAAGCTGAGCTTGCAACTCTAATGGCTACTTTGGGGGAACTCAATATTGATTCACCGATACAGAGGGAGAAGAGATCAAATTCATTGAAAGAGAAACTTGCATCTGTGGCACCACTTGTGGAAGATCTAAGAGTGAAGAAAGAGGAAAGAATGAAGCAATTTTCAGATATAAAGGCGCAAATTGAGAAGATCAGTGGGGAGATATCCGGATACAACCATCTCAACAATGCTCCAGTCAGTTCGTTAACCCTGGATGAACAAGACCTATCAGTGAGAAAGCTAACTGAATATCAAGCACATCTTCACACTCTTCAAAAAGAGAAGTCTGACCGCCTTCATAAGGTTTTGGAATGTGTAAATGAGGTTCATTCTCTTTGCGGTGTGCTTGGCTTGGATTTTGGTCAGACTGTAAGTGAAGTGGATCCTAGTTTGCACAGGACAAGCATAGAACAGTCCACAAACATCAGCAATAGCACATTTGAAGGCCTAGAACAGACCATTCAGAAGTTGAAAACAGAAAGGAAAGCTCGAATCCAGAAGCTAAAAGATATTGTAGCGTCACTCCTTGAACTTTGGAATTTGATGGACTCATCAAGAGAAGAAAGAAGTAAATTTTCAAGAATTACTTTAATTCTTGGAAATTCAGAACCAGAAATTACAGAACCAGGCTTTCTTTCAACAGATATTATTGAACAGGCATCGGCAGAAGTGGAAAGACTCACTGAATTGAAAGCAAGCAGAATGAAAGAACTTGTTATGAAAAGGAGGTCAGAATTGGAGGAAATGTGCAGAATGGCTCATATTGAACCTGATACAAGTACATCTGCTGAGAAATCAAATGCATTGATAGATTCTGGTCTCGTAGATCCATCTGAACTTCTGGCAAACATAGAAGCACAGATAGTCAAAGCTAAAGAAGAGGCTCTGAGCCGGAAAGAAATAATGGATAGGATAGATCGGTGGCTATTTGCATGTGAAGAGGAAAATTGGCTTGAAGACTACAATCAAGATGAGAACCGGTACAGTGCAGGGAGAGGTGCTCACATTAACCTTAAACGGGCAGAACGAGCTAGAGTGACTGTTAACAAAATTCCAGCAATTGTTGACAATTTGATAAACAAAACGCTGGCCTGGGAAGATGAGAAAAAGATGTTATTCCTTTATGATGGGGCACGACTGGTGTCGATACTGGAGGATTACAAAGTGAGCAGGCAGCAAAAGGAAGAGGAGAAGAAGAGATCTAGGGACCAAAAGAAGCTCCAAGATCTGCTCCTCACAGAGAAAGAAGCCATGTATGGGTCTAGACCTAGTCCTAGAAAAAGTACCAGCTTTAGGAAGCCAAATGGATATCGCCCAAATGGGAATGGATCAATGACTCCTACACCTCGTCGGAATTCAGTTGGCAGTGGAACTCCTGAGCTCTTAACACCCCGATCTTACTCTGGGCGGCAAAATGGATATTTCAAGGAAATGAGAAGATTGTCTACTACACCACTGAACTTTGTGGCCGTATCAAAAGAAGATACAATGTCATATGCGTCTGTTTGTGGTTCTGTGCTGGGATCGCCCCCTCATGATTGA

Protein Analysis

601

Amino Acids

68.11

Weight (kDa)

6.2

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01910 AT2G01910
fragaria_vesca FvH4_4g26120 FvH4_4g26120 FvH4_4g26120
malus_domestica MD16G1013200.v1.1
prunus_persica Prupe.1G338100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433691
rosa_laevigata RLG00000006738
rosa_multiflora Rmu_sc0000312.1_g000024
rosa_roxburghii Rroxscaffold_5G00375130
rosa_rugosa Rorug04G0270700.1 Rorug04G0270800.1
rosa_samantha Rh4AG325200 Rh4BG333000 Rh4CG348200 Rh4DG329600
rosa_wichuraiana Rw4G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 1706
AccIII TCCGGA 1 cut(s) 428
AciI CCGC 4 cut(s) 118, 553, 600, 1669
AclWI GGATC 5 cut(s) 638, 651, 1082, 1597, 1792
AcoI YGGCCR 1 cut(s) 1728
AcsI RAATTY 6 cut(s) 301, 799, 833, 862, 1311, 1615
AcuI CTGAAG 1 cut(s) 56
AfaI GTAC 4 cut(s) 37, 1042, 1244, 1546
AfiI CCNNNNNNNGG 4 cut(s) 562, 1279, 1518, 1554
AgeI ACCGGT 1 cut(s) 1239
AjnI CCWGG 3 cut(s) 471, 886, 1352
Alw21I GWGCWC 2 cut(s) 1264, 1644
Alw26I GTCTC 1 cut(s) 1085
AlwI GGATC 5 cut(s) 638, 651, 1082, 1597, 1792
Aor13HI TCCGGA 1 cut(s) 428
AoxI GGCC 3 cut(s) 706, 1350, 1728
ApeKI GCWGC 3 cut(s) 179, 215, 1435
ApoI RAATTY 6 cut(s) 301, 799, 833, 862, 1311, 1615
AsiGI ACCGGT 1 cut(s) 1239
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 1468
AsuHPI GGTGA 2 cut(s) 107, 267
AvaII GGWCC 1 cut(s) 1468
BaeGI GKGCMC 1 cut(s) 1396
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 1644
BarI GAAGNNNNNNTAC 2 cut(s) 1690, 1722
BbsI GAAGAC 1 cut(s) 1224
Bbv12I GWGCWC 2 cut(s) 1264, 1644
BbvI GCAGC 3 cut(s) 166, 227, 1447
BccI CCATC 5 cut(s) 32, 447, 799, 1099, 1382
BceAI ACGGC 1 cut(s) 1715
BciT130I CCWGG 3 cut(s) 473, 888, 1354
BciVI GTATCC 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 7 cut(s) 648, 710, 1293, 1464, 1524, 1530, 1536
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 3 cut(s) 346, 1460, 1486
BisI GCNGC 4 cut(s) 180, 216, 1436, 1670
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 4 cut(s) 181, 217, 1437, 1671
Bme1390I CCNGG 3 cut(s) 473, 888, 1354
Bme18I GGWCC 1 cut(s) 1468
BmgT120I GGNCC 1 cut(s) 1468
BmiI GGNNCC 3 cut(s) 333, 645, 1469
BmrFI CCNGG 3 cut(s) 473, 888, 1354
BmsI GCATC 2 cut(s) 332, 929
BoxI GACNNNNGTC 1 cut(s) 1402
BpiI GAAGAC 1 cut(s) 1224
BplI GAGNNNNNCTC 2 cut(s) 1246, 1278
BpmI CTGGAG 2 cut(s) 438, 1433
Bpu10I CCTNAGC 1 cut(s) 1638
Bpu1102I GCTNAGC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 3 cut(s) 189, 471, 1353
BsaWI WCCGGW 2 cut(s) 428, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 4 cut(s) 562, 1279, 1518, 1554
Bse118I RCCGGY 1 cut(s) 1239
Bse1I ACTGG 3 cut(s) 455, 1404, 1416
Bse3DI GCAATG 1 cut(s) 58
BseAI TCCGGA 1 cut(s) 428
BseBI CCWGG 3 cut(s) 473, 888, 1354
BseDI CCNNGG 3 cut(s) 189, 471, 1353
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 4 cut(s) 562, 1279, 1518, 1554
BseMI GCAATG 1 cut(s) 58
BseMII CTCAG 4 cut(s) 219, 1041, 1139, 1629
BseNI ACTGG 3 cut(s) 455, 1404, 1416
BseRI GAGGAG 2 cut(s) 1463, 1484
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 3 cut(s) 166, 227, 1447
BseYI CCCAGC 1 cut(s) 1780
BsgI GTGCAG 2 cut(s) 1030, 1269
BshFI GGCC 3 cut(s) 708, 1352, 1730
BshNI GGYRCC 1 cut(s) 331
BshTI ACCGGT 1 cut(s) 1239
BsiHKAI GWGCWC 2 cut(s) 1264, 1644
BsiSI CCGG 3 cut(s) 429, 1153, 1240
BslFI GGGAC 1 cut(s) 1481
BslI CCNNNNNNNGG 4 cut(s) 562, 1279, 1518, 1554
BsmAI GTCTC 1 cut(s) 1085
BsmFI GGGAC 1 cut(s) 1481
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 708, 1352, 1730
Bso31I GGTCTC 1 cut(s) 1085
Bsp1286I GDGCHC 3 cut(s) 1264, 1396, 1644
Bsp13I TCCGGA 1 cut(s) 428
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 4 cut(s) 118, 553, 600, 1669
BspANI GGCC 3 cut(s) 708, 1352, 1730
BspCNI CTCAG 4 cut(s) 220, 1042, 1140, 1630
BspEI TCCGGA 1 cut(s) 428
BspHI TCATGA 1 cut(s) 1795
BspLI GGNNCC 3 cut(s) 333, 645, 1469
BspPI GGATC 5 cut(s) 638, 651, 1082, 1597, 1792
BspT107I GGYRCC 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 1239
BsrI ACTGG 3 cut(s) 455, 1404, 1416
BssAI RCCGGY 1 cut(s) 1239
BssECI CCNNGG 3 cut(s) 189, 471, 1353
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 473, 888, 1354
Bst4CI ACNGT 4 cut(s) 631, 675, 1247, 1303
Bst6I CTCTTC 6 cut(s) 287, 537, 1134, 1194, 1440, 1451
BstAPI GCANNNNNTGC 1 cut(s) 48
BstC8I GCNNGC 7 cut(s) 6, 122, 196, 234, 958, 1350, 1433
BstDEI CTNAG 6 cut(s) 148, 228, 350, 1050, 1148, 1638
BstENI CCTNNNNNAGG 1 cut(s) 560
BstF5I GGATG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 1085
BstMWI GCNNNNNNNGC 7 cut(s) 48, 185, 224, 233, 329, 1289, 1786
BstNI CCWGG 3 cut(s) 473, 888, 1354
BstNSI RCATGY 2 cut(s) 124, 1197
BstPAI GACNNNNGTC 1 cut(s) 1402
BstSCI CCNGG 3 cut(s) 471, 886, 1352
BstSLI GKGCMC 1 cut(s) 1396
BstV1I GCAGC 3 cut(s) 166, 227, 1447
BstV2I GAAGAC 1 cut(s) 1224
BstX2I RGATCY 5 cut(s) 346, 643, 1087, 1460, 1486
BstYI RGATCY 5 cut(s) 346, 643, 1087, 1460, 1486
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 708, 1352, 1730
BtsCI GGATG 1 cut(s) 481
BtsI GCAGTG 1 cut(s) 1633
BtsIMutI CAGTG 6 cut(s) 421, 498, 942, 1252, 1633, 1715
Cac8I GCNNGC 7 cut(s) 6, 122, 196, 234, 958, 1350, 1433
CciI TCATGA 1 cut(s) 1795
CfoI GCGC 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 1239
Cfr13I GGNCC 1 cut(s) 1468
CseI GACGC 2 cut(s) 768, 1749
Csp6I GTAC 4 cut(s) 36, 1041, 1243, 1545
CspAI ACCGGT 1 cut(s) 1239
CviAII CATG 4 cut(s) 121, 1194, 1513, 1796
CviQI GTAC 4 cut(s) 36, 1041, 1243, 1545
DdeI CTNAG 6 cut(s) 148, 228, 350, 1050, 1148, 1638
EaeI YGGCCR 1 cut(s) 1728
Eam1104I CTCTTC 6 cut(s) 287, 537, 1134, 1194, 1440, 1451
EarI CTCTTC 6 cut(s) 287, 537, 1134, 1194, 1440, 1451
Ecl136II GAGCTC 1 cut(s) 1642
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 1644
Eco31I GGTCTC 1 cut(s) 1085
Eco32I GATATC 2 cut(s) 426, 1571
Eco47I GGWCC 1 cut(s) 1468
Eco53kI GAGCTC 1 cut(s) 1642
Eco57I CTGAAG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 1642
EcoNI CCTNNNNNAGG 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 1615
EcoRII CCWGG 3 cut(s) 471, 886, 1352
EcoRV GATATC 2 cut(s) 426, 1571
EcoT14I CCWWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 1644
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 4 cut(s) 124, 1197, 1516, 1799
FalI AAGNNNNNCTT 8 cut(s) 216, 248, 303, 335, 507, 539, 832, 864
FaqI GGGAC 1 cut(s) 1481
FatI CATG 4 cut(s) 120, 1193, 1512, 1795
FauNDI CATATG 1 cut(s) 1756
FblI GTMKAC 1 cut(s) 1706
Fnu4HI GCNGC 4 cut(s) 180, 216, 1436, 1670
FokI GGATG 1 cut(s) 488
FriOI GRGCYC 1 cut(s) 1644
Fsp4HI GCNGC 4 cut(s) 180, 216, 1436, 1670
FspBI CTAG 7 cut(s) 648, 710, 1293, 1464, 1524, 1530, 1536
GlaI GCGC 1 cut(s) 399
GluI GCNGC 4 cut(s) 180, 216, 1436, 1670
GsaI CCCAGC 1 cut(s) 1784
GsuI CTGGAG 2 cut(s) 438, 1433
HaeIII GGCC 3 cut(s) 708, 1352, 1730
HapII CCGG 3 cut(s) 429, 1153, 1240
HgaI GACGC 2 cut(s) 768, 1749
HhaI GCGC 1 cut(s) 400
Hin1II CATG 4 cut(s) 124, 1197, 1516, 1799
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 3 cut(s) 468, 1306, 1327
HindII GTYRAC 3 cut(s) 468, 1306, 1327
HinfI GANTC 7 cut(s) 30, 272, 753, 809, 939, 1073, 1597
HpaI GTTAAC 2 cut(s) 468, 1306
HpaII CCGG 3 cut(s) 429, 1153, 1240
HphI GGTGA 2 cut(s) 107, 267
Hpy166II GTNNAC 6 cut(s) 157, 468, 678, 1306, 1327, 1707
Hpy188III TCNNGA 8 cut(s) 429, 757, 816, 840, 1229, 1524, 1637, 1796
Hpy8I GTNNAC 6 cut(s) 157, 468, 678, 1306, 1327, 1707
Hpy99I CGWCG 1 cut(s) 1614
HpyAV CCTTC 4 cut(s) 160, 209, 566, 698
HpyCH4III ACNGT 4 cut(s) 631, 675, 1247, 1303
HpyCH4V TGCA 9 cut(s) 51, 215, 236, 323, 655, 1011, 1064, 1193, 1250
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 185, 224, 233, 329, 1289, 1786
HpyF3I CTNAG 6 cut(s) 148, 228, 350, 1050, 1148, 1638
Hsp92II CATG 4 cut(s) 124, 1197, 1516, 1799
HspAI GCGC 1 cut(s) 398
Kpn2I TCCGGA 1 cut(s) 428
KspAI GTTAAC 2 cut(s) 468, 1306
LmnI GCTCC 3 cut(s) 457, 1485, 1497
Lsp1109I GCAGC 3 cut(s) 166, 227, 1447
LweI GCATC 2 cut(s) 332, 929
MaeI CTAG 7 cut(s) 648, 710, 1293, 1464, 1524, 1530, 1536
MaeIII GTNAC 2 cut(s) 780, 1297
MfeI CAATTG 1 cut(s) 1320
MflI RGATCY 5 cut(s) 346, 643, 1087, 1460, 1486
MhlI GDGCHC 3 cut(s) 1264, 1396, 1644
MlyI GAGTC 4 cut(s) 39, 803, 933, 1591
MmeI TCCRAC 3 cut(s) 108, 114, 1592
Mph1103I ATGCAT 1 cut(s) 1066
MroI TCCGGA 1 cut(s) 428
MseI TTAA 6 cut(s) 467, 851, 1269, 1275, 1305, 1646
MspI CCGG 3 cut(s) 429, 1153, 1240
MspR9I CCNGG 3 cut(s) 473, 888, 1354
MunI CAATTG 1 cut(s) 1320
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 3 cut(s) 473, 888, 1354
MwoI GCNNNNNNNGC 7 cut(s) 48, 185, 224, 233, 329, 1289, 1786
NdeI CATATG 1 cut(s) 1756
NlaIII CATG 4 cut(s) 124, 1197, 1516, 1799
NlaIV GGNNCC 3 cut(s) 333, 645, 1469
NmuCI GTSAC 2 cut(s) 780, 1297
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 2 cut(s) 124, 1197
PaeI GCATGC 1 cut(s) 124
PagI TCATGA 1 cut(s) 1795
PceI AGGCCT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 1285
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 3 cut(s) 272, 753, 1073
PflFI GACNNNGTC 1 cut(s) 1530
PinAI ACCGGT 1 cut(s) 1239
PkrI GCNGC 4 cut(s) 181, 217, 1437, 1671
PleI GAGTC 4 cut(s) 38, 803, 933, 1591
PpsI GAGTC 4 cut(s) 38, 803, 933, 1591
PshAI GACNNNNGTC 1 cut(s) 1402
Psp124BI GAGCTC 1 cut(s) 1644
Psp6I CCWGG 3 cut(s) 471, 886, 1352
PspFI CCCAGC 1 cut(s) 1780
PspGI CCWGG 3 cut(s) 471, 886, 1352
PspN4I GGNNCC 3 cut(s) 333, 645, 1469
PspPI GGNCC 1 cut(s) 1468
PsrI GAACNNNNNNTAC 2 cut(s) 570, 602
PsuI RGATCY 5 cut(s) 346, 643, 1087, 1460, 1486
PsyI GACNNNGTC 1 cut(s) 1530
RsaI GTAC 4 cut(s) 37, 1042, 1244, 1546
RsaNI GTAC 4 cut(s) 36, 1041, 1243, 1545
SacI GAGCTC 1 cut(s) 1644
SaqAI TTAA 6 cut(s) 467, 851, 1269, 1275, 1305, 1646
SatI GCNGC 4 cut(s) 180, 216, 1436, 1670
Sau96I GGNCC 1 cut(s) 1468
SchI GAGTC 4 cut(s) 39, 803, 933, 1591
ScrFI CCNGG 3 cut(s) 473, 888, 1354
SduI GDGCHC 3 cut(s) 1264, 1396, 1644
SfaNI GCATC 2 cut(s) 332, 929
SinI GGWCC 1 cut(s) 1468
SphI GCATGC 1 cut(s) 124
SseBI AGGCCT 1 cut(s) 708
SsiI CCGC 4 cut(s) 118, 553, 600, 1669
SspI AATATT 1 cut(s) 268
SspMI CTAG 7 cut(s) 648, 710, 1293, 1464, 1524, 1530, 1536
SstI GAGCTC 1 cut(s) 1644
StuI AGGCCT 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 471, 886, 1352
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 631, 675, 1247, 1303
TaqI TCGA 2 cut(s) 751, 1406
TatI WGTACW 1 cut(s) 1040
TauI GCSGC 1 cut(s) 1672
TfiI GAWTC 3 cut(s) 272, 753, 1073
Tru1I TTAA 6 cut(s) 467, 851, 1269, 1275, 1305, 1646
Tru9I TTAA 6 cut(s) 467, 851, 1269, 1275, 1305, 1646
TscAI CASTG 6 cut(s) 421, 498, 949, 1252, 1633, 1722
TseFI GTSAC 2 cut(s) 780, 1297
TseI GCWGC 3 cut(s) 179, 215, 1435
Tsp45I GTSAC 2 cut(s) 780, 1297
TspDTI ATGAA 9 cut(s) 189, 191, 294, 389, 492, 548, 578, 980, 995
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 6 cut(s) 421, 498, 949, 1252, 1633, 1722
Tth111I GACNNNGTC 1 cut(s) 1530
VpaK11BI GGWCC 1 cut(s) 1468
XagI CCTNNNNNAGG 1 cut(s) 560
XapI RAATTY 6 cut(s) 301, 799, 833, 862, 1311, 1615
XbaI TCTAGA 1 cut(s) 1523
XceI RCATGY 2 cut(s) 124, 1197
XmiI GTMKAC 1 cut(s) 1706
XspI CTAG 7 cut(s) 648, 710, 1293, 1464, 1524, 1530, 1536
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.