Rh4BG333000

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013510
Physical Location & Seq
Forward (+)
49725782 .. 49731500
5719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG333000.1

Sequence Viewer

Length: 1686 bp
ATGTTGTTGGAGTTGGAAAGGGAATGCTTAGAAGTTTACAGGAGGAAGGTTGATGAAGCTGCCAATACCAAGGCACGCCTTCATCAGTCCGTTGCAGCAAAAGAAGCTGAGCTCGCCACTCTCATGGCTACTCTGGGGGAACTCAATATTGATTCACCGATACGGAGAGAAAAGAGATCAAGTTCTTTGAAAGAGAAACTCGCATCTGTTGCACCACTGCTAGACAATCTAAAAATGAAGAAAGAGGCACGAATGAAGCAATTTTCAGATATAAGGACACAAATTGAGAAGATCAGCGAGGAAATATCTGGTTACGATCATCTCAACAGTTCTTTGATCAATTCATTACCCCTTGATGAACATGACTTGTCATCGAGGAAGCTAACTGAATATCAAGCACATCTGCACACCCTTCAAAAGGAGAAGTCCGACCGCCTTCAGAAGGTTTTGGAATATATCAATGAGGTTCATTCTCTTTGCGGTGTGCTTGGCTTGGATTTTGGTAAGACTGTAAGTGAAGTGGATCCTAGCTTGCAGAGGACAAACACAGAACAGTCCACGAACATCAGCAATAGCACATTGGAGGGTCTAGAGCAGACCATTCTCAAGTTGAAAACCGAAAGAAAAGCTCGAATCCAGAAGCTAAAAGATGTCGTAGCATCGCTCTATGAACTTTGGAATTTGATGGACTCTTCAAAAGAAGAAATGAATAAATTTTCAAGGATTACTTACATTCTTGGAAATGCAGAACCAGAAATTACACATCCTGGCTTACTTTCAACAGATGTTATTGAACAGGCATCGGCAGAAGTGGAGAGACTCACTGAATTGAAAGCAAGTAGAATGAAAGAGCTCGTTATGAAAAGGAGGTCTGAATTAGAGGAAATATGCAGAATGACTCACATTGAACCTGATACCAACACAGCTGCTGAGAAATCAAGTGCATTGATAGATTCTGGTCTAGTAGATCCGTCCGAACTTCTGGCAAACATAGAAGCACAGATACTCAAAGTTAAAGAAGAAGCTCTAAGTCGAAAAGAAATTACGGATAGGATAGACCGGTGGCTATTTGCTTGTGAAGAGGAAAATTGGCTCGAAGACTACAATCAAGATGATAGTCGGTACAGTGCTGGGAGAGGGGTTCACATTAACCTTAAACGTGCAGAACGTGCTCGAGTGACTGTAAACAAAATTCCAGCAATTGTTGACAATTTGATAAACAAAACGCTGGCCTGGGAAAACGAGAGAGAGACATTATTTCTTTATGATGGGGTACGCTTGGTGTCAATACTAGAGGATTACAAGGTGAGCAGGCGGCAGAAGGAAGAGGAGAAGAAGCGATCTAGGGACCAAAAGAAGATCCAAGATCTTCTCCTCACAGAGAAAGAGGCCATGTATGGGTCTAGACCTAGTCCTAGAAAAAGTAATAGCTTCAGAAAACCAAATGGATATCGTGCAAATGGAAATGGATCCATGACTCCTACCCCTCGTCGGAACTCAGTTGGCAGCGGCACTCCGGAGCTCTTAACACCCCGATCTTACTCAGGGCGCCAAAATGGATATTTCAAGGAAATGAGACGATTGTCTACTGCACCACTGAACTTTGTGGCCATATCAAAAGAAGATACAATGTCATATGCTACATCTGTTTGTGGTTCAGTGTTAGGCTCCCCCCCTCGTGATTAA

Protein Analysis

561

Amino Acids

63.93

Weight (kDa)

7.14

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 2 - 476 5.7e-76 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01910 AT2G01910
fragaria_vesca FvH4_4g26120 FvH4_4g26120 FvH4_4g26120
malus_domestica MD16G1013200.v1.1
prunus_persica Prupe.1G338100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433691
rosa_laevigata RLG00000006738
rosa_multiflora Rmu_sc0000312.1_g000024
rosa_roxburghii Rroxscaffold_5G00375130
rosa_rugosa Rorug04G0270700.1 Rorug04G0270800.1
rosa_samantha Rh4AG325200 Rh4BG333000 Rh4CG348200 Rh4DG329600
rosa_wichuraiana Rw4G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1548
AccI GTMKAC 1 cut(s) 1586
AccIII TCCGGA 1 cut(s) 1516
AciI CCGC 4 cut(s) 433, 480, 1315, 1509
AclWI GGATC 6 cut(s) 518, 531, 962, 1354, 1464, 1477
AcoI YGGCCR 1 cut(s) 1608
AcsI RAATTY 3 cut(s) 679, 713, 1191
AcuI CTGAAG 2 cut(s) 422, 1417
AcyI GRCGYC 1 cut(s) 1549
AfaI GTAC 2 cut(s) 1124, 1275
AfiI CCNNNNNNNGG 4 cut(s) 418, 442, 1398, 1491
AgeI ACCGGT 1 cut(s) 1059
AjnI CCWGG 2 cut(s) 766, 1232
Alw21I GWGCWC 4 cut(s) 114, 855, 1174, 1524
Alw26I GTCTC 3 cut(s) 811, 1244, 1570
AlwI GGATC 6 cut(s) 518, 531, 962, 1354, 1464, 1477
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 1 cut(s) 1173
Aor13HI TCCGGA 1 cut(s) 1516
AoxI GGCC 3 cut(s) 1230, 1389, 1608
ApeKI GCWGC 4 cut(s) 59, 95, 926, 1506
ApoI RAATTY 3 cut(s) 679, 713, 1191
AsiGI ACCGGT 1 cut(s) 1059
AspLEI GCGC 1 cut(s) 1551
AspS9I GGNCC 1 cut(s) 1348
AsuHPI GGTGA 2 cut(s) 147, 1318
AvaI CYCGRG 1 cut(s) 1173
AvaII GGWCC 1 cut(s) 1348
BalI TGGCCA 1 cut(s) 1610
BamHI GGATCC 2 cut(s) 523, 1469
BanI GGYRCC 1 cut(s) 1548
BanII GRGCYC 3 cut(s) 114, 855, 1524
BarI GAAGNNNNNNTAC 2 cut(s) 987, 1019
BauI CACGAG 1 cut(s) 1677
BbsI GAAGAC 1 cut(s) 1104
Bbv12I GWGCWC 4 cut(s) 114, 855, 1174, 1524
BbvI GCAGC 4 cut(s) 46, 107, 913, 1518
BccI CCATC 2 cut(s) 679, 1262
BciT130I CCWGG 2 cut(s) 768, 1234
BclI TGATCA 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 811, 1244, 1570
BfaI CTAG 9 cut(s) 221, 528, 590, 962, 1292, 1344, 1404, 1410, 1416
BfoI RGCGCY 1 cut(s) 1552
BglII AGATCT 1 cut(s) 1366
BisI GCNGC 6 cut(s) 60, 96, 927, 1316, 1507, 1510
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 6 cut(s) 61, 97, 928, 1317, 1508, 1511
Bme1390I CCNGG 2 cut(s) 768, 1234
Bme18I GGWCC 1 cut(s) 1348
BmeT110I CYCGRG 1 cut(s) 1173
BmgT120I GGNCC 1 cut(s) 1348
BmiI GGNNCC 5 cut(s) 525, 1349, 1471, 1550, 1669
BmrFI CCNGG 2 cut(s) 768, 1234
BmsI GCATC 3 cut(s) 212, 668, 809
BoxI GACNNNNGTC 1 cut(s) 1582
BpiI GAAGAC 1 cut(s) 1104
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 1549
BsaJI CCNNGG 2 cut(s) 69, 1233
BsaWI WCCGGW 2 cut(s) 1059, 1516
Bsc4I CCNNNNNNNGG 4 cut(s) 418, 442, 1398, 1491
Bse118I RCCGGY 1 cut(s) 1059
BseAI TCCGGA 1 cut(s) 1516
BseBI CCWGG 2 cut(s) 768, 1234
BseDI CCNNGG 2 cut(s) 69, 1233
BseGI GGATG 1 cut(s) 763
BseLI CCNNNNNNNGG 4 cut(s) 418, 442, 1398, 1491
BseMII CTCAG 4 cut(s) 99, 921, 1512, 1557
BseRI GAGGAG 2 cut(s) 1343, 1364
BseXI GCAGC 4 cut(s) 46, 107, 913, 1518
BseYI CCCAGC 1 cut(s) 1130
BsgI GTGCAG 3 cut(s) 389, 1182, 1575
Bsh1285I CGRYCG 1 cut(s) 433
BshFI GGCC 3 cut(s) 1232, 1391, 1610
BshNI GGYRCC 1 cut(s) 1548
BshTI ACCGGT 1 cut(s) 1059
BsiEI CGRYCG 1 cut(s) 433
BsiHKAI GWGCWC 4 cut(s) 114, 855, 1174, 1524
BsiHKCI CYCGRG 1 cut(s) 1173
BsiSI CCGG 2 cut(s) 1060, 1517
BslFI GGGAC 1 cut(s) 1361
BslI CCNNNNNNNGG 4 cut(s) 418, 442, 1398, 1491
BsmAI GTCTC 3 cut(s) 811, 1244, 1570
BsmBI CGTCTC 1 cut(s) 1570
BsmFI GGGAC 1 cut(s) 1361
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 3 cut(s) 1232, 1391, 1610
BsoBI CYCGRG 1 cut(s) 1173
Bsp1286I GDGCHC 4 cut(s) 114, 855, 1174, 1524
Bsp13I TCCGGA 1 cut(s) 1516
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 4 cut(s) 433, 480, 1315, 1509
BspANI GGCC 3 cut(s) 1232, 1391, 1610
BspCNI CTCAG 4 cut(s) 100, 922, 1511, 1556
BspEI TCCGGA 1 cut(s) 1516
BspLI GGNNCC 5 cut(s) 525, 1349, 1471, 1550, 1669
BspPI GGATC 6 cut(s) 518, 531, 962, 1354, 1464, 1477
BspT107I GGYRCC 1 cut(s) 1548
BsrFI RCCGGY 1 cut(s) 1059
BssAI RCCGGY 1 cut(s) 1059
BssECI CCNNGG 2 cut(s) 69, 1233
BssNI GRCGYC 1 cut(s) 1549
BssSI CACGAG 1 cut(s) 1677
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 1677
Bst2UI CCWGG 2 cut(s) 768, 1234
Bst4CI ACNGT 5 cut(s) 329, 511, 555, 1127, 1183
Bst6I CTCTTC 3 cut(s) 697, 1074, 1320
BstACI GRCGYC 1 cut(s) 1549
BstAPI GCANNNNNTGC 2 cut(s) 209, 1169
BstC8I GCNNGC 5 cut(s) 76, 114, 533, 1230, 1313
BstDEI CTNAG 6 cut(s) 28, 108, 930, 1028, 1498, 1543
BstENI CCTNNNNNAGG 2 cut(s) 416, 440
BstF5I GGATG 1 cut(s) 763
BstH2I RGCGCY 1 cut(s) 1552
BstHHI GCGC 1 cut(s) 1551
BstMAI GTCTC 3 cut(s) 811, 1244, 1570
BstMCI CGRYCG 1 cut(s) 433
BstMWI GCNNNNNNNGC 4 cut(s) 104, 113, 209, 1169
BstNI CCWGG 2 cut(s) 768, 1234
BstPAI GACNNNNGTC 1 cut(s) 1582
BstSCI CCNGG 2 cut(s) 766, 1232
BstV1I GCAGC 4 cut(s) 46, 107, 913, 1518
BstV2I GAAGAC 1 cut(s) 1104
BstX2I RGATCY 5 cut(s) 523, 967, 1359, 1366, 1469
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 5 cut(s) 523, 967, 1359, 1366, 1469
BsuRI GGCC 3 cut(s) 1232, 1391, 1610
BtgZI GCGATG 1 cut(s) 645
BtsCI GGATG 1 cut(s) 763
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 5 cut(s) 215, 822, 1132, 1595, 1665
Cac8I GCNNGC 5 cut(s) 76, 114, 533, 1230, 1313
CaiI CAGNNNCTG 1 cut(s) 929
CfoI GCGC 1 cut(s) 1551
Cfr10I RCCGGY 1 cut(s) 1059
Cfr13I GGNCC 1 cut(s) 1348
Csp6I GTAC 2 cut(s) 1123, 1274
CspAI ACCGGT 1 cut(s) 1059
CviAII CATG 4 cut(s) 124, 362, 1393, 1474
CviQI GTAC 2 cut(s) 1123, 1274
DdeI CTNAG 6 cut(s) 28, 108, 930, 1028, 1498, 1543
DinI GGCGCC 1 cut(s) 1550
EaeI YGGCCR 1 cut(s) 1608
Eam1104I CTCTTC 3 cut(s) 697, 1074, 1320
EarI CTCTTC 3 cut(s) 697, 1074, 1320
Ecl136II GAGCTC 3 cut(s) 112, 853, 1522
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 3 cut(s) 114, 855, 1524
Eco32I GATATC 1 cut(s) 1451
Eco47I GGWCC 1 cut(s) 1348
Eco53kI GAGCTC 3 cut(s) 112, 853, 1522
Eco57I CTGAAG 2 cut(s) 422, 1417
Eco88I CYCGRG 1 cut(s) 1173
EcoICRI GAGCTC 3 cut(s) 112, 853, 1522
EcoNI CCTNNNNNAGG 2 cut(s) 416, 440
EcoRII CCWGG 2 cut(s) 766, 1232
EcoRV GATATC 1 cut(s) 1451
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 3 cut(s) 114, 855, 1524
EgeI GGCGCC 1 cut(s) 1550
EheI GGCGCC 1 cut(s) 1550
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 1570
FaeI CATG 4 cut(s) 127, 365, 1396, 1477
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 1 cut(s) 1361
FatI CATG 4 cut(s) 123, 361, 1392, 1473
FauNDI CATATG 1 cut(s) 1636
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 1586
Fnu4HI GCNGC 6 cut(s) 60, 96, 927, 1316, 1507, 1510
FokI GGATG 1 cut(s) 750
FriOI GRGCYC 3 cut(s) 114, 855, 1524
Fsp4HI GCNGC 6 cut(s) 60, 96, 927, 1316, 1507, 1510
FspBI CTAG 9 cut(s) 221, 528, 590, 962, 1292, 1344, 1404, 1410, 1416
GlaI GCGC 1 cut(s) 1550
GluI GCNGC 6 cut(s) 60, 96, 927, 1316, 1507, 1510
GsaI CCCAGC 1 cut(s) 1134
HaeII RGCGCY 1 cut(s) 1552
HaeIII GGCC 3 cut(s) 1232, 1391, 1610
HapII CCGG 2 cut(s) 1060, 1517
HhaI GCGC 1 cut(s) 1551
Hin1I GRCGYC 1 cut(s) 1549
Hin1II CATG 4 cut(s) 127, 365, 1396, 1477
Hin6I GCGC 1 cut(s) 1549
HinP1I GCGC 1 cut(s) 1549
HincII GTYRAC 1 cut(s) 1207
HindII GTYRAC 1 cut(s) 1207
HinfI GANTC 7 cut(s) 152, 633, 689, 819, 898, 953, 1477
HpaII CCGG 2 cut(s) 1060, 1517
HphI GGTGA 2 cut(s) 147, 1318
Hpy166II GTNNAC 6 cut(s) 37, 558, 1144, 1186, 1207, 1587
Hpy188I TCNGA 7 cut(s) 268, 430, 441, 874, 976, 1436, 1494
Hpy188III TCNNGA 6 cut(s) 590, 637, 1109, 1404, 1517, 1679
Hpy8I GTNNAC 6 cut(s) 37, 558, 1144, 1186, 1207, 1587
Hpy99I CGWCG 1 cut(s) 1494
HpyAV CCTTC 6 cut(s) 40, 89, 422, 436, 446, 1315
HpyCH4III ACNGT 5 cut(s) 329, 511, 555, 1127, 1183
HpyCH4IV ACGT 2 cut(s) 1159, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 113, 209, 1169
HpyF3I CTNAG 6 cut(s) 28, 108, 930, 1028, 1498, 1543
HpySE526I ACGT 2 cut(s) 1159, 1168
Hsp92I GRCGYC 1 cut(s) 1549
Hsp92II CATG 4 cut(s) 127, 365, 1396, 1477
HspAI GCGC 1 cut(s) 1549
KasI GGCGCC 1 cut(s) 1548
Kpn2I TCCGGA 1 cut(s) 1516
Ksp22I TGATCA 1 cut(s) 336
LmnI GCTCC 2 cut(s) 1519, 1673
Lsp1109I GCAGC 4 cut(s) 46, 107, 913, 1518
LweI GCATC 3 cut(s) 212, 668, 809
MaeI CTAG 9 cut(s) 221, 528, 590, 962, 1292, 1344, 1404, 1410, 1416
MaeII ACGT 2 cut(s) 1159, 1168
MaeIII GTNAC 2 cut(s) 311, 1177
MfeI CAATTG 1 cut(s) 1200
MflI RGATCY 5 cut(s) 523, 967, 1359, 1366, 1469
MhlI GDGCHC 4 cut(s) 114, 855, 1174, 1524
MlsI TGGCCA 1 cut(s) 1610
MluNI TGGCCA 1 cut(s) 1610
Mly113I GGCGCC 1 cut(s) 1549
MlyI GAGTC 4 cut(s) 683, 813, 892, 1471
MmeI TCCRAC 2 cut(s) 453, 1472
Mox20I TGGCCA 1 cut(s) 1610
MroI TCCGGA 1 cut(s) 1516
MscI TGGCCA 1 cut(s) 1610
MseI TTAA 5 cut(s) 1014, 1149, 1155, 1526, 1684
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 1 cut(s) 1610
MspA1I CMGCKG 2 cut(s) 926, 1509
MspI CCGG 2 cut(s) 1060, 1517
MspR9I CCNGG 2 cut(s) 768, 1234
MunI CAATTG 1 cut(s) 1200
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 2 cut(s) 768, 1234
MwoI GCNNNNNNNGC 4 cut(s) 104, 113, 209, 1169
NarI GGCGCC 1 cut(s) 1549
NdeI CATATG 1 cut(s) 1636
NlaIII CATG 4 cut(s) 127, 365, 1396, 1477
NlaIV GGNNCC 5 cut(s) 525, 1349, 1471, 1550, 1669
NmuCI GTSAC 1 cut(s) 1177
PaeR7I CTCGAG 1 cut(s) 1173
PcsI WCGNNNNNNNCGW 1 cut(s) 1165
PctI GAATGC 1 cut(s) 29
PfeI GAWTC 3 cut(s) 152, 633, 953
PflFI GACNNNGTC 1 cut(s) 1410
PinAI ACCGGT 1 cut(s) 1059
PkrI GCNGC 6 cut(s) 61, 97, 928, 1317, 1508, 1511
PleI GAGTC 4 cut(s) 683, 813, 892, 1471
PluTI GGCGCC 1 cut(s) 1552
PpsI GAGTC 4 cut(s) 683, 813, 892, 1471
PshAI GACNNNNGTC 1 cut(s) 1582
Psp124BI GAGCTC 3 cut(s) 114, 855, 1524
Psp6I CCWGG 2 cut(s) 766, 1232
PspFI CCCAGC 1 cut(s) 1130
PspGI CCWGG 2 cut(s) 766, 1232
PspN4I GGNNCC 5 cut(s) 525, 1349, 1471, 1550, 1669
PspPI GGNCC 1 cut(s) 1348
PspXI VCTCGAGB 1 cut(s) 1173
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 5 cut(s) 523, 967, 1359, 1366, 1469
PsyI GACNNNGTC 1 cut(s) 1410
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 2 cut(s) 1124, 1275
RsaNI GTAC 2 cut(s) 1123, 1274
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 3 cut(s) 114, 855, 1524
SaqAI TTAA 5 cut(s) 1014, 1149, 1155, 1526, 1684
SatI GCNGC 6 cut(s) 60, 96, 927, 1316, 1507, 1510
Sau96I GGNCC 1 cut(s) 1348
SchI GAGTC 4 cut(s) 683, 813, 892, 1471
ScrFI CCNGG 2 cut(s) 768, 1234
SduI GDGCHC 4 cut(s) 114, 855, 1174, 1524
SfaNI GCATC 3 cut(s) 212, 668, 809
SfoI GGCGCC 1 cut(s) 1550
Sfr274I CTCGAG 1 cut(s) 1173
SinI GGWCC 1 cut(s) 1348
SlaI CTCGAG 1 cut(s) 1173
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 605, 1173
SmoI CTYRAG 2 cut(s) 605, 1173
SsiI CCGC 4 cut(s) 433, 480, 1315, 1509
SspDI GGCGCC 1 cut(s) 1548
SspI AATATT 1 cut(s) 148
SspMI CTAG 9 cut(s) 221, 528, 590, 962, 1292, 1344, 1404, 1410, 1416
SstI GAGCTC 3 cut(s) 114, 855, 1524
StyD4I CCNGG 2 cut(s) 766, 1232
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 5 cut(s) 329, 511, 555, 1127, 1183
TaiI ACGT 2 cut(s) 1162, 1171
TaqI TCGA 5 cut(s) 374, 631, 1033, 1095, 1174
TauI GCSGC 2 cut(s) 1318, 1512
TfiI GAWTC 3 cut(s) 152, 633, 953
Tru1I TTAA 5 cut(s) 1014, 1149, 1155, 1526, 1684
Tru9I TTAA 5 cut(s) 1014, 1149, 1155, 1526, 1684
TscAI CASTG 5 cut(s) 222, 829, 1132, 1602, 1665
TseFI GTSAC 1 cut(s) 1177
TseI GCWGC 4 cut(s) 59, 95, 926, 1506
Tsp45I GTSAC 1 cut(s) 1177
TspGWI ACGGA 4 cut(s) 79, 178, 960, 1061
TspRI CASTG 5 cut(s) 222, 829, 1132, 1602, 1665
Tth111I GACNNNGTC 1 cut(s) 1410
VpaK11BI GGWCC 1 cut(s) 1348
XagI CCTNNNNNAGG 2 cut(s) 416, 440
XapI RAATTY 3 cut(s) 679, 713, 1191
XbaI TCTAGA 2 cut(s) 589, 1403
XhoI CTCGAG 1 cut(s) 1173
XmiI GTMKAC 1 cut(s) 1586
XspI CTAG 9 cut(s) 221, 528, 590, 962, 1292, 1344, 1404, 1410, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.