Prupe.1G355400_v2.0.a1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012551
Physical Location & Seq
Forward (+)
32987234 .. 32990135
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G355400.1

Sequence Viewer

Length: 1920 bp
ATGGGGTGCGCCGCCTCAAAGGTGGAGGATCTTCCGGCGGTGGCTTTATGCCGTGACCGGTGCAAGTTCCTGGAGGAAGCTATCAGCCAAACCCAAGCCATGTCCGATGCTCACGTGGCTTACTTGGCCTCCCTCAGGACACTTGGCCCCTCTCTCCACCGTTTCTTCGATGATGTTTATACCATTCAGAATCATTGTAATTGTAATGGACCACCAGAATCAGAATCAGAATCCGACTCGACCTCGCACCTCCAACTCCCTTCCGACTCCGAATCCGAAACCGAACTAAAGGGTCCCCGTAAACGGTCGGACGAAGCGCCAACTCCAGTTAATGATGGTGATGAATATTACAGGAATCTAGGCGGCGGCTTGGCTTCCAAACCCCCTCCTCCGCCACCGTCTCCAATCAGCTCCACTTGGGATTTGTTTAATTTCTTTGACGCCTACGACAGGGAGAGATACCCACCATCCCCACTTTATTCTTCTACTCATTTGGAGCCTGAAGAAGCCGCTACTCCTACTGCTGACAAAATCAAACATAACAATATTAATATTAATAATGACATTGGACAGCAGGAAGAACAACAGACTAGGCCGAAAGAGAAGAGTAAGAAGACGATTCATTTGGCCATGTCTGTCAAACACAACAAACAGAACGAACAGAACAAACAGGGTGTCTCTGCAGAAGCAGAGGCCATGCGAGAGCTTCACCTTCTCTTTGACAGAGCATCTGAGTCCGGCAATCAAGTTTTGAAGTCGCTGCAGTCTCACCATCACACAATTTCTCTTCATCAGGAGTATGGCTTTGGTAACCGTGAAGGCTTGGTGAGCTCACAGAGCCTATCTTACACTCTGAAGACGCTCTTCTTGTGGGAAAAGAAACTTTACGACGAAGTCAAGGCTGAGGAAAGATTGCGCGTAACGCATGAGAAGAAGAGTCGAATGCTGAAACAATTGGAACACAATAAGGCCACCGCTGAGTCTCCTAAAATTGAATCTGTTCGGACTGCACTTCACAATGTTTCAACAAAAATGAAAATTGCAATTCATATTGTTGACAGAATATCAGTTACCATAAACAAGTTGAGGGAAGAGGAGTTGTGGCCGCAGATTGTTGAATTCATTCACAGATTACTTGGAATGTGGAAAGCTATGGTAGAATGTCACAAGAGTCAATACCAAGCAATTGCGGAAGCCTCCAAGAGTTTGGATGCAATTGCAATATCCAACGCGAAGTTGAACGGTGCGCATGATCTTGAAACTGCAATTCAACTCAAGTTAGAGCTTCAAAACTGGAATGTATGCTTCTCCAATTGGATTGCCACCCAAAAGGGGTATGTAAAAGCACTAAATGGTTGGCTTCTCAGATGTCTTCTACAGGAACCATCACCGCAGACAGATGAGGAGGGTGATGCAGCAGTAGTAGTACCTCTGTCTCCTGGTAGACTCGGTGCACCCCCAGCTTTTGTGATATGTAATCAGTGGTCTCAAGCTATGGATAGACTCTCAGGGAAGGAAGTTATTGAAGCTATCCGGGGATTTTACACTCGTATACATTTGCTTTTAATGGAACACCACGTTGATGCAGAAACATTGCTGCAAAGAATGGCAATTGAAAACAAAGATGTAGCAAGGAAACTAAGACTTGTGGAGGAGGGGAAGGAGGAGGAGGAGGAGCAAAGGACAATGCAAAAGGTGCTGATGCGAGGTAGAGGAAAAAAGAATATTATTGGTTTGAGTTTGCAGGCAGCACAAGAGGAGGGTGGAGAGAGAGAGAATAATATTGGATCAAGGAATCTAGTATGGGATCTGAAGCAGACATTAATGGCCATGGAAAAATTCACTGCAAACTCCCTGAAAGCATACGAAGAGCTTGATGCACACATTGAAAAAGTCAACAGCCCCGGCTTGTGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

640

Amino Acids

72.12

Weight (kDa)

6.04

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20530
fragaria_vesca FvH4_2g37590 FvH4_2g37590
malus_domestica MD08G1003500.v1.1 MD15G1003100.v1.1
prunus_persica Prupe.1G355400_v2.0.a1
pyrus_communis pycom08g00270 pycom15g00200 pycom15g00210
rosa_chinensis RchiOBHm_Chr6g0304141
rosa_laevigata RLG00000011011
rosa_multiflora Rmu_ssc0000218.1_g000006
rosa_roxburghii Rroxscaffold_7G00164030
rosa_rugosa Rorug06G0328000
rosa_samantha Rh6AG442000 Rh6BG460800 Rh6CG454200 Rh6DG441400
rosa_wichuraiana Rw6G038310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1248
AccI GTMKAC 2 cut(s) 1444, 1552
AccII CGCG 2 cut(s) 918, 1232
AclWI GGATC 3 cut(s) 36, 1795, 1815
AcoI YGGCCR 3 cut(s) 627, 1103, 1827
AcsI RAATTY 2 cut(s) 1118, 1838
AcuI CTGAAG 3 cut(s) 522, 875, 1832
AcvI CACGTG 1 cut(s) 115
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 1 cut(s) 1428
AfiI CCNNNNNNNGG 4 cut(s) 135, 303, 450, 1332
AgeI ACCGGT 1 cut(s) 57
AjnI CCWGG 2 cut(s) 69, 1438
Alw21I GWGCWC 2 cut(s) 833, 1456
Alw26I GTCTC 6 cut(s) 405, 682, 771, 987, 1440, 1491
Alw44I GTGCAC 1 cut(s) 1452
AlwI GGATC 3 cut(s) 36, 1795, 1815
AoxI GGCC 8 cut(s) 126, 145, 593, 627, 693, 969, 1103, 1827
ApaLI GTGCAC 1 cut(s) 1452
ApeKI GCWGC 4 cut(s) 760, 1415, 1597, 1748
ApoI RAATTY 2 cut(s) 1118, 1838
ArsI GACNNNNNNTTYG 4 cut(s) 607, 639, 660, 692
AseI ATTAAT 3 cut(s) 549, 555, 1823
AsiGI ACCGGT 1 cut(s) 57
Asp700I GAANNNNTTC 2 cut(s) 999, 1122
AspLEI GCGC 4 cut(s) 11, 319, 918, 1249
AspS9I GGNCC 3 cut(s) 146, 209, 293
AsuC2I CCSGG 2 cut(s) 1535, 1905
AsuHPI GGTGA 6 cut(s) 350, 701, 761, 838, 1380, 1421
AvaII GGWCC 2 cut(s) 209, 293
AxyI CCTNAGG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 1456
BalI TGGCCA 2 cut(s) 629, 1829
BanII GRGCYC 1 cut(s) 833
BbrPI CACGTG 1 cut(s) 115
BbsI GAAGAC 3 cut(s) 620, 863, 1364
Bbv12I GWGCWC 2 cut(s) 833, 1456
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 4 cut(s) 747, 1427, 1584, 1760
BccI CCATC 4 cut(s) 329, 475, 780, 1393
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 2 cut(s) 71, 1440
BcnI CCSGG 2 cut(s) 1535, 1905
BcoDI GTCTC 6 cut(s) 405, 682, 771, 987, 1440, 1491
BfaI CTAG 3 cut(s) 359, 591, 1799
BfmI CTRYAG 3 cut(s) 681, 761, 1376
BfoI RGCGCY 1 cut(s) 320
BisI GCNGC 9 cut(s) 12, 364, 367, 510, 761, 1106, 1416, 1598, 1749
BlsI GCNGC 9 cut(s) 13, 365, 368, 511, 762, 1107, 1417, 1599, 1750
Bme1390I CCNGG 4 cut(s) 71, 1440, 1535, 1905
Bme18I GGWCC 2 cut(s) 209, 293
BmgT120I GGNCC 3 cut(s) 146, 209, 293
BmiI GGNNCC 5 cut(s) 148, 294, 295, 498, 1383
BmrFI CCNGG 4 cut(s) 71, 1440, 1535, 1905
BmsI GCATC 7 cut(s) 97, 737, 1201, 1402, 1573, 1692, 1867
BpiI GAAGAC 3 cut(s) 620, 863, 1364
BpmI CTGGAG 2 cut(s) 92, 309
Bpu10I CCTNAGC 1 cut(s) 903
BpuEI CTTGAG 2 cut(s) 1259, 1473
BpuMI CCSGG 2 cut(s) 1535, 1905
BsaAI YACGTR 1 cut(s) 115
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 1491
BsaJI CCNNGG 3 cut(s) 1534, 1830, 1903
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 303, 450, 1332
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 2 cut(s) 326, 1298
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 1592
BseBI CCWGG 2 cut(s) 71, 1440
BseDI CCNNGG 3 cut(s) 1534, 1830, 1903
BseGI GGATG 2 cut(s) 467, 1216
BseLI CCNNNNNNNGG 4 cut(s) 135, 303, 450, 1332
BseMI GCAATG 1 cut(s) 1592
BseMII CTCAG 6 cut(s) 148, 723, 894, 969, 1378, 1521
BseNI ACTGG 2 cut(s) 326, 1298
BseRI GAGGAG 9 cut(s) 378, 1109, 1418, 1667, 1679, 1682, 1685, 1688, 1772
BseSI GKGCMC 1 cut(s) 1456
BseXI GCAGC 4 cut(s) 747, 1427, 1584, 1760
BseYI CCCAGC 1 cut(s) 1459
BsgI GTGCAG 1 cut(s) 993
Bsh1236I CGCG 2 cut(s) 918, 1232
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 8 cut(s) 128, 147, 595, 629, 695, 971, 1105, 1829
BshTI ACCGGT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 833, 1456
BsiSI CCGG 5 cut(s) 35, 58, 738, 1534, 1905
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 135, 303, 450, 1332
BsmAI GTCTC 6 cut(s) 405, 682, 771, 987, 1440, 1491
BsmBI CGTCTC 1 cut(s) 405
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 8 cut(s) 128, 147, 595, 629, 695, 971, 1105, 1829
Bso31I GGTCTC 1 cut(s) 1491
Bsp1286I GDGCHC 2 cut(s) 833, 1456
Bsp143I GATC 4 cut(s) 28, 1252, 1787, 1807
Bsp19I CCATGG 1 cut(s) 1830
BspANI GGCC 8 cut(s) 128, 147, 595, 629, 695, 971, 1105, 1829
BspCNI CTCAG 6 cut(s) 147, 724, 895, 970, 1377, 1520
BspFNI CGCG 2 cut(s) 918, 1232
BspLI GGNNCC 5 cut(s) 148, 294, 295, 498, 1383
BspMAI CTGCAG 2 cut(s) 685, 765
BspPI GGATC 3 cut(s) 36, 1795, 1815
BspQI GCTCTTC 2 cut(s) 869, 1863
BspTNI GGTCTC 1 cut(s) 1491
BsrDI GCAATG 1 cut(s) 1592
BsrFI RCCGGY 1 cut(s) 57
BsrI ACTGG 2 cut(s) 326, 1298
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 3 cut(s) 1534, 1830, 1903
BssMI GATC 4 cut(s) 28, 1252, 1787, 1807
BssNAI GTATAC 1 cut(s) 1553
BssNI GRCGYC 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 1830
Bst1107I GTATAC 1 cut(s) 1553
Bst2UI CCWGG 2 cut(s) 71, 1440
Bst4CI ACNGT 5 cut(s) 161, 306, 399, 815, 1244
Bst6I CTCTTC 6 cut(s) 599, 792, 869, 929, 1086, 1863
BstACI GRCGYC 1 cut(s) 441
BstAPI GCANNNNNTGC 1 cut(s) 1696
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 1746
BstDEI CTNAG 7 cut(s) 134, 732, 903, 978, 1364, 1507, 1640
BstDSI CCRYGG 1 cut(s) 1830
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 2 cut(s) 133, 448
BstF5I GGATG 2 cut(s) 467, 1216
BstFNI CGCG 2 cut(s) 918, 1232
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 4 cut(s) 11, 319, 918, 1249
BstKTI GATC 4 cut(s) 31, 1255, 1790, 1810
BstMAI GTCTC 6 cut(s) 405, 682, 771, 987, 1440, 1491
BstMBI GATC 4 cut(s) 28, 1252, 1787, 1807
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 7 cut(s) 116, 125, 828, 837, 922, 1460, 1696
BstNI CCWGG 2 cut(s) 71, 1440
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 4 cut(s) 69, 1438, 1533, 1903
BstSFI CTRYAG 3 cut(s) 681, 761, 1376
BstSLI GKGCMC 1 cut(s) 1456
BstUI CGCG 2 cut(s) 918, 1232
BstV1I GCAGC 4 cut(s) 747, 1427, 1584, 1760
BstV2I GAAGAC 3 cut(s) 620, 863, 1364
BstX2I RGATCY 2 cut(s) 28, 1807
BstXI CCANNNNNNTGG 1 cut(s) 1207
BstYI RGATCY 2 cut(s) 28, 1807
BstZ17I GTATAC 1 cut(s) 1553
Bsu36I CCTNAGG 1 cut(s) 134
BsuRI GGCC 8 cut(s) 128, 147, 595, 629, 695, 971, 1105, 1829
BtgI CCRYGG 1 cut(s) 1830
BtsCI GGATG 2 cut(s) 467, 1216
BtsI GCAGTG 1 cut(s) 1842
BtsIMutI CAGTG 2 cut(s) 1487, 1842
Cac8I GCNNGC 1 cut(s) 1746
CfoI GCGC 4 cut(s) 11, 319, 918, 1249
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 3 cut(s) 146, 209, 293
CseI GACGC 2 cut(s) 449, 868
Csp6I GTAC 1 cut(s) 1427
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 6 cut(s) 100, 631, 697, 926, 1250, 1831
CviQI GTAC 1 cut(s) 1427
DdeI CTNAG 7 cut(s) 134, 732, 903, 978, 1364, 1507, 1640
DpnI GATC 4 cut(s) 30, 1254, 1789, 1809
DpnII GATC 4 cut(s) 28, 1252, 1787, 1807
EaeI YGGCCR 3 cut(s) 627, 1103, 1827
Eam1104I CTCTTC 6 cut(s) 599, 792, 869, 929, 1086, 1863
EarI CTCTTC 6 cut(s) 599, 792, 869, 929, 1086, 1863
EciI GGCGGA 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 831
Eco130I CCWWGG 1 cut(s) 1830
Eco24I GRGCYC 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 1491
Eco47I GGWCC 2 cut(s) 209, 293
Eco53kI GAGCTC 1 cut(s) 831
Eco57I CTGAAG 3 cut(s) 522, 875, 1832
Eco72I CACGTG 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 134
Eco91I GGTNACC 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 831
EcoNI CCTNNNNNAGG 2 cut(s) 133, 448
EcoO109I RGGNCCY 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 2 cut(s) 69, 1438
EcoT14I CCWWGG 1 cut(s) 1830
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 1 cut(s) 1830
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 6 cut(s) 103, 634, 700, 929, 1253, 1834
FalI AAGNNNNNCTT 2 cut(s) 848, 880
FaqI GGGAC 1 cut(s) 279
FatI CATG 6 cut(s) 99, 630, 696, 925, 1249, 1830
FblI GTMKAC 2 cut(s) 1444, 1552
Fnu4HI GCNGC 9 cut(s) 12, 364, 367, 510, 761, 1106, 1416, 1598, 1749
FokI GGATG 2 cut(s) 454, 1223
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 9 cut(s) 12, 364, 367, 510, 761, 1106, 1416, 1598, 1749
FspAI RTGCGCAY 1 cut(s) 1248
FspBI CTAG 3 cut(s) 359, 591, 1799
FspI TGCGCA 1 cut(s) 1248
GlaI GCGC 4 cut(s) 10, 318, 917, 1248
GluI GCNGC 9 cut(s) 12, 364, 367, 510, 761, 1106, 1416, 1598, 1749
GsaI CCCAGC 1 cut(s) 1463
GsuI CTGGAG 2 cut(s) 92, 309
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 8 cut(s) 128, 147, 595, 629, 695, 971, 1105, 1829
HapII CCGG 5 cut(s) 35, 58, 738, 1534, 1905
HgaI GACGC 2 cut(s) 449, 868
HhaI GCGC 4 cut(s) 11, 319, 918, 1249
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 6 cut(s) 103, 634, 700, 929, 1253, 1834
Hin6I GCGC 4 cut(s) 9, 317, 916, 1247
HinP1I GCGC 4 cut(s) 9, 317, 916, 1247
HincII GTYRAC 2 cut(s) 1057, 1897
HindII GTYRAC 2 cut(s) 1057, 1897
HpaII CCGG 5 cut(s) 35, 58, 738, 1534, 1905
HphI GGTGA 6 cut(s) 350, 701, 761, 838, 1380, 1421
Hpy166II GTNNAC 6 cut(s) 302, 1057, 1445, 1454, 1553, 1897
Hpy188III TCNNGA 3 cut(s) 136, 794, 1256
Hpy8I GTNNAC 6 cut(s) 302, 1057, 1445, 1454, 1553, 1897
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 5 cut(s) 270, 722, 812, 1507, 1654
HpyCH4III ACNGT 5 cut(s) 161, 306, 399, 815, 1244
HpyCH4IV ACGT 2 cut(s) 114, 1578
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 125, 828, 837, 922, 1460, 1696
HpyF3I CTNAG 7 cut(s) 134, 732, 903, 978, 1364, 1507, 1640
HpySE526I ACGT 2 cut(s) 114, 1578
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 6 cut(s) 103, 634, 700, 929, 1253, 1834
HspAI GCGC 4 cut(s) 9, 317, 916, 1247
KflI GGGWCCC 1 cut(s) 293
Kzo9I GATC 4 cut(s) 28, 1252, 1787, 1807
LguI GCTCTTC 2 cut(s) 869, 1863
LmnI GCTCC 3 cut(s) 416, 496, 1675
Lsp1109I GCAGC 4 cut(s) 747, 1427, 1584, 1760
LweI GCATC 7 cut(s) 97, 737, 1201, 1402, 1573, 1692, 1867
MaeI CTAG 3 cut(s) 359, 591, 1799
MaeII ACGT 2 cut(s) 114, 1578
MaeIII GTNAC 5 cut(s) 53, 809, 919, 1069, 1163
MalI GATC 4 cut(s) 30, 1254, 1789, 1809
MboI GATC 4 cut(s) 28, 1252, 1787, 1807
MfeI CAATTG 5 cut(s) 953, 1185, 1215, 1312, 1611
MflI RGATCY 2 cut(s) 28, 1807
MhlI GDGCHC 2 cut(s) 833, 1456
MlsI TGGCCA 2 cut(s) 629, 1829
MluNI TGGCCA 2 cut(s) 629, 1829
MlyI GAGTC 8 cut(s) 230, 260, 743, 946, 989, 1180, 1440, 1497
MmeI TCCRAC 5 cut(s) 258, 277, 288, 288, 1251
Mox20I TGGCCA 2 cut(s) 629, 1829
MroXI GAANNNNTTC 2 cut(s) 999, 1122
MscI TGGCCA 2 cut(s) 629, 1829
MseI TTAA 6 cut(s) 330, 429, 549, 555, 1565, 1823
MslI CAYNNNNRTG 1 cut(s) 1581
Msp20I TGGCCA 2 cut(s) 629, 1829
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 5 cut(s) 35, 58, 738, 1534, 1905
MspR9I CCNGG 4 cut(s) 71, 1440, 1535, 1905
MunI CAATTG 5 cut(s) 953, 1185, 1215, 1312, 1611
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 2 cut(s) 71, 1440
MvnI CGCG 2 cut(s) 918, 1232
MwoI GCNNNNNNNGC 7 cut(s) 116, 125, 828, 837, 922, 1460, 1696
NciI CCSGG 2 cut(s) 1535, 1905
NcoI CCATGG 1 cut(s) 1830
NdeII GATC 4 cut(s) 28, 1252, 1787, 1807
NlaIII CATG 6 cut(s) 103, 634, 700, 929, 1253, 1834
NlaIV GGNNCC 5 cut(s) 148, 294, 295, 498, 1383
NmuCI GTSAC 2 cut(s) 53, 1163
NsbI TGCGCA 1 cut(s) 1248
PciSI GCTCTTC 2 cut(s) 869, 1863
PctI GAATGC 1 cut(s) 948
PdmI GAANNNNTTC 2 cut(s) 999, 1122
PfeI GAWTC 9 cut(s) 190, 218, 224, 230, 272, 355, 619, 995, 1795
PflFI GACNNNGTC 1 cut(s) 893
PfoI TCCNGGA 1 cut(s) 69
PinAI ACCGGT 1 cut(s) 57
PkrI GCNGC 9 cut(s) 13, 365, 368, 511, 762, 1107, 1417, 1599, 1750
PleI GAGTC 8 cut(s) 230, 260, 742, 945, 988, 1179, 1440, 1497
PmaCI CACGTG 1 cut(s) 115
PmlI CACGTG 1 cut(s) 115
PpsI GAGTC 8 cut(s) 230, 260, 742, 945, 988, 1179, 1440, 1497
Ppu21I YACGTR 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 293
PshBI ATTAAT 3 cut(s) 549, 555, 1823
Psp124BI GAGCTC 1 cut(s) 833
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 69, 1438
PspCI CACGTG 1 cut(s) 115
PspEI GGTNACC 1 cut(s) 809
PspFI CCCAGC 1 cut(s) 1459
PspGI CCWGG 2 cut(s) 69, 1438
PspN4I GGNNCC 5 cut(s) 148, 294, 295, 498, 1383
PspPI GGNCC 3 cut(s) 146, 209, 293
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 2 cut(s) 685, 765
PsuI RGATCY 2 cut(s) 28, 1807
PsyI GACNNNGTC 1 cut(s) 893
RsaI GTAC 1 cut(s) 1428
RsaNI GTAC 1 cut(s) 1427
RseI CAYNNNNRTG 1 cut(s) 1581
SacI GAGCTC 1 cut(s) 833
SapI GCTCTTC 2 cut(s) 869, 1863
SaqAI TTAA 6 cut(s) 330, 429, 549, 555, 1565, 1823
SatI GCNGC 9 cut(s) 12, 364, 367, 510, 761, 1106, 1416, 1598, 1749
Sau3AI GATC 4 cut(s) 28, 1252, 1787, 1807
Sau96I GGNCC 3 cut(s) 146, 209, 293
SchI GAGTC 8 cut(s) 230, 260, 743, 946, 989, 1180, 1440, 1497
ScrFI CCNGG 4 cut(s) 71, 1440, 1535, 1905
SduI GDGCHC 2 cut(s) 833, 1456
SfaNI GCATC 7 cut(s) 97, 737, 1201, 1402, 1573, 1692, 1867
SfcI CTRYAG 3 cut(s) 681, 761, 1376
SinI GGWCC 2 cut(s) 209, 293
SmiMI CAYNNNNRTG 1 cut(s) 1581
SmlI CTYRAG 2 cut(s) 1274, 1488
SmoI CTYRAG 2 cut(s) 1274, 1488
SspI AATATT 5 cut(s) 347, 547, 553, 1726, 1783
SspMI CTAG 3 cut(s) 359, 591, 1799
SstI GAGCTC 1 cut(s) 833
StyD4I CCNGG 4 cut(s) 69, 1438, 1533, 1903
StyI CCWWGG 1 cut(s) 1830
TaaI ACNGT 5 cut(s) 161, 306, 399, 815, 1244
TaiI ACGT 2 cut(s) 117, 1581
TaqI TCGA 3 cut(s) 168, 239, 940
TauI GCSGC 5 cut(s) 14, 366, 369, 512, 1108
TfiI GAWTC 9 cut(s) 190, 218, 224, 230, 272, 355, 619, 995, 1795
Tru1I TTAA 6 cut(s) 330, 429, 549, 555, 1565, 1823
Tru9I TTAA 6 cut(s) 330, 429, 549, 555, 1565, 1823
TscAI CASTG 2 cut(s) 1487, 1849
TseFI GTSAC 2 cut(s) 53, 1163
TseI GCWGC 4 cut(s) 760, 1415, 1597, 1748
Tsp45I GTSAC 2 cut(s) 53, 1163
TspDTI ATGAA 6 cut(s) 357, 611, 779, 1037, 1049, 1111
TspRI CASTG 2 cut(s) 1487, 1849
Tth111I GACNNNGTC 1 cut(s) 893
VneI GTGCAC 1 cut(s) 1452
VpaK11BI GGWCC 2 cut(s) 209, 293
VspI ATTAAT 3 cut(s) 549, 555, 1823
XagI CCTNNNNNAGG 2 cut(s) 133, 448
XapI RAATTY 2 cut(s) 1118, 1838
XmiI GTMKAC 2 cut(s) 1444, 1552
XmnI GAANNNNTTC 2 cut(s) 999, 1122
XspI CTAG 3 cut(s) 359, 591, 1799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.