pycom08g00270

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012551
Physical Location & Seq
Forward (+)
281101 .. 283342
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00270.1

Sequence Viewer

Length: 1959 bp
ATGGGTTGCGCCGCCTCAAAGTTGGAGAATCTTCCGGCTGTGGCATTGTGCCGAGACCGGTGCAAGTACCTGGAGGAAGCTCTCAGCCAAGCCCAAGTTCTGGCTGATGCCCACGTCGCTTACTTGGCCTCCCTCAGGACCCTTGGCCCCGCTCTCCACCGTTTCTTCCATGACATTCCTGCAGGAAATGAACCGCCGCCGCCACCAGAATCAGACTCGCACCTCCAATTCCCTTCCGACTCCGAATCCGAATCCAAAGAGGACCACACCGTTAAACAGTTGGGTGAAACGCCGACACCAGTTTCTGAGCGTGATCAATATTTCAGGGAAGGGGAGGGGGAGACCTACAAAAATTCAGCCGGCGGACTGACTTCCGAACCCCTTCCTCCGCCACCGCCTCCGCTTAGCTCCGCTTGGGATTTCCTTAGTCTGTTTGATGCCTACGAGAGGGATATTTACCTAGCACCACTTTATTCTTGCACTAATTCGGATCTTGAAGAAGCTGCTCATACTCCTGCACTTGCTGCTGCCGCTGCTGACAAAATCAAAGTCGATAATTGTGGCGGCAATAACGATATCAATGTTAAACACAATACTAAAAATATTGGAGATGATCAGGAAGAGGAGAGTAGGTCAGGGAAGAGTAAGACGACTCATATGGCGACGTCGACGGCAACTCCTCCGTCTGTCTCTGTCTCGGTCCCTGTGCTAGAAGAAACAGAGGCGATGCGAGAGCTTAATCTTCTATTTGACAGGGCGTCTGAGTCCGGGAATCAAGTTTTATTGTCGCTGCAGTCTCATCATCACACGATTTCTCTTGATCAGGAAGCAGAGTATGGATTTGATGACCGTGAGGGCTTGGTGATGAGCTCGCAAAACCTTTCTTACACTCTGAAGACGCTCTTCCTGTGGGAAAAGAAACTTTACCACGAAGTCAAGGCTGAGGAAAGATTGCGCGTTACGCACGAGAAGAAGAGTAGAATGCTGAAACATTTAGAACATGGCAACGGTAATACTACTAGTGCTGCTGAGGCTCCCAAACTCAACTCCCTTCGCAGTGCAGTCCGCGATCTTTTAACAAAAATGAAAATTGCAATTCAGATTGTTGACAAAATATCAATTACCATCAACAAGTTGAGGGACGAGGAGCTATGGCCGCAAATTATCGAATTCATCAACAGGTTACTCGACATGTGGAAAGCTATGGTAGAATGTCACAAGAGTCAGTACCGAGCAATGGTGGAAGCCAAGGGTTTACATGGCGTTGCAATATCCAAAGCAAAGTTGAGCGGCGCACATAATGCTGAAACTGCAATTCGACTCAAGTTAGAGCTTCAAAACTGGAATGTGTGCTTCTCCAATTGGATTGTCGCCCAAAAGGTGTATGTAAAAGCATTAAATGGATGGGTTCAAAGATGTCTTCTACACGAACCAAATGTAGCCGATCAAGAATTAACAGCAGACGAGGCTATTGGAGCACCGCCTGTTTTTGTGATATGTAATGAGTGGTCACAAGCTATGGATAGACTCTCAGATGAGGAAGTTGTTGAAGCCATCGGGGGATTCACAACTAGTATACATACGCTTTTAATGGAAGGCAACAATATTAATGTGGAAATGGTGCAGCAAAGGATGGTAGCTGAAAACAAAGATGTAGCAAGGAAAGTAAAGTTTTTGGAGGCAGAGGAGGGGAAGGAACAGAAGATACTGCATAAGCTGATGCGCGGTAGAGGAGGAAAAAAGAACGTTGCTTCGAGTAGTTTGCGAGCAGGAGAAGATGAGTCGAACAGAGAGAGACTGCAGCAACAGAGTCGGAGTGACAGGAATATTAATCTAGTGTGGGATTTGAAGCAGACATTGATGGCGATGGAGAAATTCACCGCAAACTCATTGCAAGCATACAATGAGATTCATGCACACATTGAACTACAACAAGTCAGCTGCAGGCAATCCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

653

Amino Acids

72.88

Weight (kDa)

5.61

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 57 5.4e-22 Protein of unknown function (DUF630)
DUF632 PF04782 261 - 527 1e-83 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20530
fragaria_vesca FvH4_2g37590 FvH4_2g37590
malus_domestica MD08G1003500.v1.1 MD15G1003100.v1.1
prunus_persica Prupe.1G355400_v2.0.a1
pyrus_communis pycom08g00270 pycom15g00200 pycom15g00210
rosa_chinensis RchiOBHm_Chr6g0304141
rosa_laevigata RLG00000011011
rosa_multiflora Rmu_ssc0000218.1_g000006
rosa_roxburghii Rroxscaffold_7G00164030
rosa_rugosa Rorug06G0328000
rosa_samantha Rh6AG442000 Rh6BG460800 Rh6CG454200 Rh6DG441400
rosa_wichuraiana Rw6G038310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 668
AccB7I CCANNNNNTGG 1 cut(s) 100
AccBSI CCGCTC 2 cut(s) 152, 1290
AccI GTMKAC 2 cut(s) 668, 1576
AccII CGCG 3 cut(s) 957, 1068, 1725
AccIII TCCGGA 1 cut(s) 1952
AclI AACGTT 1 cut(s) 1746
AclWI GGATC 1 cut(s) 498
AcoI YGGCCR 1 cut(s) 1154
AcsI RAATTY 3 cut(s) 352, 1169, 1874
AcuI CTGAAG 1 cut(s) 914
AcyI GRCGYC 2 cut(s) 665, 758
AfaI GTAC 2 cut(s) 68, 1229
AfiI CCNNNNNNNGG 4 cut(s) 100, 135, 447, 1237
AflIII ACRYGT 1 cut(s) 1191
AgeI ACCGGT 1 cut(s) 57
AgsI TTSAA 6 cut(s) 497, 1337, 1412, 1550, 1849, 1925
AhdI GACNNNNNGTC 1 cut(s) 757
AhlI ACTAGT 2 cut(s) 1019, 1571
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 69
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
Alw21I GWGCWC 2 cut(s) 872, 1480
Alw26I GTCTC 6 cut(s) 48, 335, 694, 700, 801, 1789
AlwI GGATC 1 cut(s) 498
AlwNI CAGNNNCTG 1 cut(s) 305
Aor13HI TCCGGA 1 cut(s) 1952
AoxI GGCC 3 cut(s) 126, 145, 1154
ApeKI GCWGC 9 cut(s) 503, 524, 527, 533, 790, 1025, 1624, 1801, 1941
ApoI RAATTY 3 cut(s) 352, 1169, 1874
ArsI GACNNNNNNTTYG 2 cut(s) 534, 566
AseI ATTAAT 2 cut(s) 1608, 1830
AsiGI ACCGGT 1 cut(s) 57
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 4 cut(s) 11, 957, 1295, 1725
AspS9I GGNCC 4 cut(s) 138, 146, 262, 700
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 3 cut(s) 296, 874, 1870
AvaII GGWCC 3 cut(s) 138, 262, 700
AxyI CCTNAGG 1 cut(s) 134
BanII GRGCYC 1 cut(s) 872
BauI CACGAG 1 cut(s) 965
BbsI GAAGAC 2 cut(s) 902, 1412
Bbv12I GWGCWC 2 cut(s) 872, 1480
BbvCI CCTCAGC 2 cut(s) 942, 1029
BbvI GCAGC 9 cut(s) 490, 511, 514, 520, 777, 1012, 1636, 1813, 1928
BccI CCATC 6 cut(s) 1133, 1398, 1562, 1627, 1855, 1861
BceAI ACGGC 1 cut(s) 687
BcgI CGANNNNNNTGC 6 cut(s) 42, 76, 1744, 1778, 1793, 1827
BciT130I CCWGG 1 cut(s) 71
BclI TGATCA 3 cut(s) 313, 613, 820
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 6 cut(s) 48, 335, 694, 700, 801, 1789
BcuI ACTAGT 2 cut(s) 1019, 1571
BfaI CTAG 5 cut(s) 461, 710, 1020, 1572, 1835
BfmI CTRYAG 4 cut(s) 180, 791, 1799, 1942
BlpI GCTNAGC 1 cut(s) 404
Bme1390I CCNGG 2 cut(s) 71, 769
Bme18I GGWCC 3 cut(s) 138, 262, 700
BmeRI GACNNNNNGTC 1 cut(s) 757
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 138, 146, 262, 700
BmiI GGNNCC 4 cut(s) 140, 148, 702, 1035
BmrFI CCNGG 2 cut(s) 71, 769
BmsI GCATC 4 cut(s) 97, 427, 717, 1710
BpiI GAAGAC 2 cut(s) 902, 1412
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 2 cut(s) 942, 1029
Bpu1102I GCTNAGC 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 1307
BpuMI CCSGG 1 cut(s) 769
BsaHI GRCGYC 2 cut(s) 665, 758
BsaI GGTCTC 2 cut(s) 48, 335
BsaJI CCNNGG 2 cut(s) 142, 1248
BsaWI WCCGGW 2 cut(s) 57, 1952
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 617, 647, 661, 691
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 135, 447, 1237
Bse118I RCCGGY 2 cut(s) 57, 359
Bse1I ACTGG 2 cut(s) 299, 1346
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 2 cut(s) 1242, 1889
BseAI TCCGGA 1 cut(s) 1952
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 2 cut(s) 142, 1248
BseGI GGATG 2 cut(s) 1409, 1638
BseLI CCNNNNNNNGG 4 cut(s) 100, 135, 447, 1237
BseMI GCAATG 2 cut(s) 1242, 1889
BseMII CTCAG 7 cut(s) 97, 148, 297, 753, 933, 1020, 1545
BseNI ACTGG 2 cut(s) 299, 1346
BseRI GAGGAG 5 cut(s) 638, 669, 1160, 1700, 1746
BseXI GCAGC 9 cut(s) 490, 511, 514, 520, 777, 1012, 1636, 1813, 1928
BsgI GTGCAG 3 cut(s) 501, 1080, 1643
Bsh1236I CGCG 3 cut(s) 957, 1068, 1725
BshFI GGCC 3 cut(s) 128, 147, 1156
BshTI ACCGGT 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 872, 1480
BsiSI CCGG 5 cut(s) 35, 58, 360, 768, 1953
BslFI GGGAC 2 cut(s) 686, 1154
BslI CCNNNNNNNGG 4 cut(s) 100, 135, 447, 1237
BsmAI GTCTC 6 cut(s) 48, 335, 694, 700, 801, 1789
BsmFI GGGAC 2 cut(s) 686, 1154
BsmI GAATGC 1 cut(s) 987
BsnI GGCC 3 cut(s) 128, 147, 1156
Bso31I GGTCTC 2 cut(s) 48, 335
Bsp1286I GDGCHC 2 cut(s) 872, 1480
Bsp13I TCCGGA 1 cut(s) 1952
Bsp143I GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
Bsp1720I GCTNAGC 1 cut(s) 404
BspANI GGCC 3 cut(s) 128, 147, 1156
BspCNI CTCAG 7 cut(s) 96, 147, 298, 754, 934, 1021, 1544
BspEI TCCGGA 1 cut(s) 1952
BspFNI CGCG 3 cut(s) 957, 1068, 1725
BspLI GGNNCC 4 cut(s) 140, 148, 702, 1035
BspMAI CTGCAG 4 cut(s) 184, 795, 1803, 1946
BspPI GGATC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 908
BspTNI GGTCTC 2 cut(s) 48, 335
BsrBI CCGCTC 2 cut(s) 152, 1290
BsrDI GCAATG 2 cut(s) 1242, 1889
BsrFI RCCGGY 2 cut(s) 57, 359
BsrI ACTGG 2 cut(s) 299, 1346
BssAI RCCGGY 2 cut(s) 57, 359
BssECI CCNNGG 2 cut(s) 142, 1248
BssMI GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
BssNAI GTATAC 1 cut(s) 1577
BssNI GRCGYC 2 cut(s) 665, 758
BssSI CACGAG 1 cut(s) 965
BssT1I CCWWGG 2 cut(s) 142, 1248
Bst1107I GTATAC 1 cut(s) 1577
Bst2BI CACGAG 1 cut(s) 965
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 5 cut(s) 161, 271, 279, 851, 1010
Bst6I CTCTTC 4 cut(s) 615, 635, 908, 968
BstACI GRCGYC 2 cut(s) 665, 758
BstAPI GCANNNNNTGC 2 cut(s) 524, 1301
BstC8I GCNNGC 5 cut(s) 361, 872, 1767, 1896, 1946
BstDEI CTNAG 9 cut(s) 83, 134, 306, 404, 425, 762, 942, 1029, 1531
BstENI CCTNNNNNAGG 2 cut(s) 133, 445
BstF5I GGATG 2 cut(s) 1409, 1638
BstFNI CGCG 3 cut(s) 957, 1068, 1725
BstHHI GCGC 4 cut(s) 11, 957, 1295, 1725
BstKTI GATC 6 cut(s) 316, 493, 616, 823, 1072, 1447
BstMAI GTCTC 6 cut(s) 48, 335, 694, 700, 801, 1789
BstMBI GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 1195
BstSCI CCNGG 2 cut(s) 69, 767
BstSFI CTRYAG 4 cut(s) 180, 791, 1799, 1942
BstUI CGCG 3 cut(s) 957, 1068, 1725
BstV1I GCAGC 9 cut(s) 490, 511, 514, 520, 777, 1012, 1636, 1813, 1928
BstV2I GAAGAC 2 cut(s) 902, 1412
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BstZ17I GTATAC 1 cut(s) 1577
Bsu36I CCTNAGG 1 cut(s) 134
BsuRI GGCC 3 cut(s) 128, 147, 1156
BtgZI GCGATG 2 cut(s) 740, 1880
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 1409, 1638
BtsI GCAGTG 1 cut(s) 1063
BtsIMutI CAGTG 1 cut(s) 1063
Cac8I GCNNGC 5 cut(s) 361, 872, 1767, 1896, 1946
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 4 cut(s) 11, 957, 1295, 1725
Cfr10I RCCGGY 2 cut(s) 57, 359
Cfr13I GGNCC 4 cut(s) 138, 146, 262, 700
CseI GACGC 2 cut(s) 747, 907
Csp6I GTAC 2 cut(s) 67, 1228
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 5 cut(s) 170, 1001, 1192, 1259, 1913
CviQI GTAC 2 cut(s) 67, 1228
DdeI CTNAG 9 cut(s) 83, 134, 306, 404, 425, 762, 942, 1029, 1531
DpnI GATC 6 cut(s) 315, 492, 615, 822, 1071, 1446
DpnII GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
DriI GACNNNNNGTC 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 1154
Eam1104I CTCTTC 4 cut(s) 615, 635, 908, 968
Eam1105I GACNNNNNGTC 1 cut(s) 757
EarI CTCTTC 4 cut(s) 615, 635, 908, 968
EciI GGCGGA 2 cut(s) 378, 378
Ecl136II GAGCTC 1 cut(s) 870
Eco130I CCWWGG 2 cut(s) 142, 1248
Eco24I GRGCYC 1 cut(s) 872
Eco31I GGTCTC 2 cut(s) 48, 335
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 3 cut(s) 138, 262, 700
Eco53kI GAGCTC 1 cut(s) 870
Eco57I CTGAAG 1 cut(s) 914
Eco81I CCTNAGG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 870
EcoNI CCTNNNNNAGG 2 cut(s) 133, 445
EcoO109I RGGNCCY 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 1169
EcoRII CCWGG 1 cut(s) 69
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 2 cut(s) 142, 1248
EcoT38I GRGCYC 1 cut(s) 872
ErhI CCWWGG 2 cut(s) 142, 1248
FaeI CATG 5 cut(s) 173, 1004, 1195, 1262, 1916
FalI AAGNNNNNCTT 2 cut(s) 887, 919
FaqI GGGAC 2 cut(s) 686, 1154
FatI CATG 5 cut(s) 169, 1000, 1191, 1258, 1912
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 657
FbaI TGATCA 3 cut(s) 313, 613, 820
FblI GTMKAC 2 cut(s) 668, 1576
FokI GGATG 2 cut(s) 1416, 1645
FriOI GRGCYC 1 cut(s) 872
FspBI CTAG 5 cut(s) 461, 710, 1020, 1572, 1835
GlaI GCGC 4 cut(s) 10, 956, 1294, 1724
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 3 cut(s) 128, 147, 1156
HapII CCGG 5 cut(s) 35, 58, 360, 768, 1953
HgaI GACGC 2 cut(s) 747, 907
HhaI GCGC 4 cut(s) 11, 957, 1295, 1725
Hin1I GRCGYC 2 cut(s) 665, 758
Hin1II CATG 5 cut(s) 173, 1004, 1195, 1262, 1916
Hin6I GCGC 4 cut(s) 9, 955, 1293, 1723
HinP1I GCGC 4 cut(s) 9, 955, 1293, 1723
HincII GTYRAC 2 cut(s) 669, 1108
HindII GTYRAC 2 cut(s) 669, 1108
HpaII CCGG 5 cut(s) 35, 58, 360, 768, 1953
HphI GGTGA 3 cut(s) 296, 874, 1870
Hpy166II GTNNAC 4 cut(s) 669, 1108, 1256, 1577
Hpy188III TCNNGA 7 cut(s) 136, 494, 617, 818, 824, 1448, 1953
Hpy8I GTNNAC 4 cut(s) 669, 1108, 1256, 1577
Hpy99I CGWCG 4 cut(s) 119, 667, 670, 673
HpyAV CCTTC 6 cut(s) 243, 323, 392, 1061, 1589, 1687
HpyCH4III ACNGT 5 cut(s) 161, 271, 279, 851, 1010
HpyCH4IV ACGT 3 cut(s) 114, 665, 1746
HpyF3I CTNAG 9 cut(s) 83, 134, 306, 404, 425, 762, 942, 1029, 1531
HpySE526I ACGT 3 cut(s) 114, 665, 1746
Hsp92I GRCGYC 2 cut(s) 665, 758
Hsp92II CATG 5 cut(s) 173, 1004, 1195, 1262, 1916
HspAI GCGC 4 cut(s) 9, 955, 1293, 1723
Kpn2I TCCGGA 1 cut(s) 1952
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 3 cut(s) 313, 613, 820
Kzo9I GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
LguI GCTCTTC 1 cut(s) 908
LmnI GCTCC 4 cut(s) 413, 1039, 1147, 1475
Lsp1109I GCAGC 9 cut(s) 490, 511, 514, 520, 777, 1012, 1636, 1813, 1928
LweI GCATC 4 cut(s) 97, 427, 717, 1710
MaeI CTAG 5 cut(s) 461, 710, 1020, 1572, 1835
MaeII ACGT 3 cut(s) 114, 665, 1746
MaeIII GTNAC 5 cut(s) 958, 1182, 1214, 1509, 1817
MalI GATC 6 cut(s) 315, 492, 615, 822, 1071, 1446
MbiI CCGCTC 2 cut(s) 152, 1290
MboI GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
MfeI CAATTG 1 cut(s) 1360
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 2 cut(s) 872, 1480
MlyI GAGTC 9 cut(s) 209, 233, 646, 773, 1231, 1314, 1521, 1790, 1819
MmeI TCCRAC 2 cut(s) 261, 1793
MroI TCCGGA 1 cut(s) 1952
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 9 cut(s) 273, 585, 738, 1076, 1397, 1454, 1589, 1608, 1830
MspA1I CMGCKG 2 cut(s) 533, 1941
MspI CCGG 5 cut(s) 35, 58, 360, 768, 1953
MspR9I CCNGG 2 cut(s) 71, 769
MunI CAATTG 1 cut(s) 1360
Mva1269I GAATGC 1 cut(s) 987
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 3 cut(s) 957, 1068, 1725
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 769
NdeI CATATG 1 cut(s) 657
NdeII GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 5 cut(s) 173, 1004, 1195, 1262, 1916
NlaIV GGNNCC 4 cut(s) 140, 148, 702, 1035
NmeAIII GCCGAG 1 cut(s) 77
NmuCI GTSAC 3 cut(s) 1214, 1509, 1817
NspI RCATGY 1 cut(s) 1195
PciI ACATGT 1 cut(s) 1191
PciSI GCTCTTC 1 cut(s) 908
PctI GAATGC 1 cut(s) 987
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 7 cut(s) 28, 209, 245, 251, 772, 1563, 1909
PflMI CCANNNNNTGG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 767
PinAI ACCGGT 1 cut(s) 57
PleI GAGTC 9 cut(s) 209, 233, 646, 772, 1230, 1314, 1521, 1789, 1818
PpsI GAGTC 9 cut(s) 209, 233, 646, 772, 1230, 1314, 1521, 1789, 1818
PpuMI RGGWCCY 1 cut(s) 138
PscI ACATGT 1 cut(s) 1191
PshBI ATTAAT 2 cut(s) 1608, 1830
Psp124BI GAGCTC 1 cut(s) 872
Psp1406I AACGTT 1 cut(s) 1746
Psp5II RGGWCCY 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 4 cut(s) 140, 148, 702, 1035
PspPI GGNCC 4 cut(s) 138, 146, 262, 700
PspPPI RGGWCCY 1 cut(s) 138
PsrI GAACNNNNNNTAC 2 cut(s) 1689, 1721
PstI CTGCAG 4 cut(s) 184, 795, 1803, 1946
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 1941
RsaI GTAC 2 cut(s) 68, 1229
RsaNI GTAC 2 cut(s) 67, 1228
SacI GAGCTC 1 cut(s) 872
SalI GTCGAC 1 cut(s) 667
SapI GCTCTTC 1 cut(s) 908
SaqAI TTAA 9 cut(s) 273, 585, 738, 1076, 1397, 1454, 1589, 1608, 1830
Sau3AI GATC 6 cut(s) 313, 490, 613, 820, 1069, 1444
Sau96I GGNCC 4 cut(s) 138, 146, 262, 700
SbfI CCTGCAGG 1 cut(s) 184
SchI GAGTC 9 cut(s) 209, 233, 646, 773, 1231, 1314, 1521, 1790, 1819
ScrFI CCNGG 2 cut(s) 71, 769
SdaI CCTGCAGG 1 cut(s) 184
SduI GDGCHC 2 cut(s) 872, 1480
SfaNI GCATC 4 cut(s) 97, 427, 717, 1710
SfcI CTRYAG 4 cut(s) 180, 791, 1799, 1942
SgrDI CGTCGACG 1 cut(s) 667
SinI GGWCC 3 cut(s) 138, 262, 700
SmlI CTYRAG 1 cut(s) 1322
SmoI CTYRAG 1 cut(s) 1322
SpeI ACTAGT 2 cut(s) 1019, 1571
Sse8387I CCTGCAGG 1 cut(s) 184
SspI AATATT 4 cut(s) 320, 604, 1606, 1828
SspMI CTAG 5 cut(s) 461, 710, 1020, 1572, 1835
SstI GAGCTC 1 cut(s) 872
StyD4I CCNGG 2 cut(s) 69, 767
StyI CCWWGG 2 cut(s) 142, 1248
TaaI ACNGT 5 cut(s) 161, 271, 279, 851, 1010
TaiI ACGT 3 cut(s) 117, 668, 1749
TaqI TCGA 7 cut(s) 552, 668, 1167, 1188, 1318, 1754, 1784
TaqII GACCGA 1 cut(s) 688
TauI GCSGC 7 cut(s) 14, 199, 202, 533, 567, 1159, 1293
TfiI GAWTC 7 cut(s) 28, 209, 245, 251, 772, 1563, 1909
Tru1I TTAA 9 cut(s) 273, 585, 738, 1076, 1397, 1454, 1589, 1608, 1830
Tru9I TTAA 9 cut(s) 273, 585, 738, 1076, 1397, 1454, 1589, 1608, 1830
TscAI CASTG 1 cut(s) 1063
TseFI GTSAC 3 cut(s) 1214, 1509, 1817
TseI GCWGC 9 cut(s) 503, 524, 527, 533, 790, 1025, 1624, 1801, 1941
Tsp45I GTSAC 3 cut(s) 1214, 1509, 1817
TspDTI ATGAA 4 cut(s) 204, 1100, 1162, 1901
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 1 cut(s) 1063
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 3 cut(s) 138, 262, 700
VspI ATTAAT 2 cut(s) 1608, 1830
XagI CCTNNNNNAGG 2 cut(s) 133, 445
XapI RAATTY 3 cut(s) 352, 1169, 1874
XceI RCATGY 1 cut(s) 1195
XmiI GTMKAC 2 cut(s) 668, 1576
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 5 cut(s) 461, 710, 1020, 1572, 1835
ZraI GACGTC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.