Prupe.3G139700_v2.0.a1

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0022334
Physical Location & Seq
Reverse (-)
14740340 .. 14746666
6327 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G139700.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 660 bp
ATGTGCCGCGATCTCAGGCCTTTCGTCCAGCACTTGCTGTCATGGGGTCGCCTGAGGAGCTCCAAATTACCGGTGGTCATGGGGTCACCTGACTCCTCTTGTCCCTCTGTGCTTCCGCCCCTGGACATGGCAGTCATCTTGCCTGACTCGCAACCAAGCACCCGTCTTCACGACCCTCTCTTGCCCTGGCTCTGGTCGATAAAGCAAGCTCTTGAGGATTGGAACTCGGGAAACGATTCCGGGTCGGATCTCACCAAGCTTCTGTCAGATTGCATCGCTGAATTCAAGGACAATGATCAGTACCGAGACGACATCAGATTCCTCAAAATTTGGTTCTTCTATATGGATTTCAGTGAAGATTTTGAAAGCGTTTTTCAGAAAATGTGGGAGAGTAAGATTTGTGTTGGCAACTATTTGCTTTATGTCTGGTATGAATCCTTTCTTGAATCCAAAGGAAAATTGTACGATGCGAGCATGGTTTATCAGATGGGTATTTCGAGGAATGCTAAACCAGTTGAGTGGGTGAAGAAGGTACATGCCTTGTTTCTTGAAAGGATGTCTGAACTAGTTTCTTCAGCTCAGACTAGTGGCAATGATGAATCGATTCCATTTGGGATCCTCATTAACCCATGGTCCAGCTCCACTATGAAAGTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

220

Amino Acids

25.27

Weight (kDa)

5.11

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022334)

Species Orthologous Gene IDs
malus_domestica MD08G1245300.v1.1 MD15G1045400.v1.1
prunus_persica Prupe.3G139700_v2.0.a1 Prupe.3G139700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccII CGCG 1 cut(s) 9
AciI CCGC 2 cut(s) 7, 116
AclWI GGATC 3 cut(s) 255, 610, 623
AcsI RAATTY 2 cut(s) 281, 327
AcuI CTGAAG 1 cut(s) 558
AfaI GTAC 3 cut(s) 302, 464, 534
AfiI CCNNNNNNNGG 2 cut(s) 127, 192
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 4 cut(s) 286, 365, 446, 551
AhlI ACTAGT 2 cut(s) 565, 584
AjnI CCWGG 2 cut(s) 120, 185
AluBI AGCT 5 cut(s) 60, 209, 259, 578, 639
AluI AGCT 5 cut(s) 60, 209, 259, 578, 639
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 3 cut(s) 255, 610, 623
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 1 cut(s) 17
ApoI RAATTY 2 cut(s) 281, 327
AsiGI ACCGGT 1 cut(s) 70
Asp700I GAANNNNTTC 3 cut(s) 235, 438, 603
AspS9I GGNCC 1 cut(s) 633
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 3 cut(s) 78, 244, 535
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 633
AxyI CCTNAGG 1 cut(s) 53
BamHI GGATCC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 1 cut(s) 481
BciT130I CCWGG 2 cut(s) 122, 187
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 300
BcuI ACTAGT 2 cut(s) 565, 584
BfaI CTAG 2 cut(s) 566, 585
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 3 cut(s) 122, 187, 241
Bme18I GGWCC 1 cut(s) 633
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 633
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 3 cut(s) 122, 187, 241
BmsI GCATC 2 cut(s) 282, 457
BpiI GAAGAC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 241
Bsa29I ATCGAT 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 120, 185, 629
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 192
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 512
Bse21I CCTNAGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 598
BseBI CCWGG 2 cut(s) 122, 187
BseCI ATCGAT 1 cut(s) 602
BseDI CCNNGG 3 cut(s) 120, 185, 629
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 127, 192
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 3 cut(s) 28, 44, 593
BseNI ACTGG 1 cut(s) 512
BseRI GAGGAG 2 cut(s) 70, 85
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 19
BshTI ACCGGT 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 2 cut(s) 71, 240
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 2 cut(s) 127, 192
BsmAI GTCTC 1 cut(s) 300
BsmBI CGTCTC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 4 cut(s) 10, 247, 295, 615
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 2 cut(s) 7, 116
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 3 cut(s) 27, 45, 592
BspDI ATCGAT 1 cut(s) 602
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 617
BspPI GGATC 3 cut(s) 255, 610, 623
BsrDI GCAATG 1 cut(s) 598
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 1 cut(s) 512
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 120, 185, 629
BssMI GATC 4 cut(s) 10, 247, 295, 615
BssT1I CCWWGG 1 cut(s) 629
Bst2UI CCWGG 2 cut(s) 122, 187
BstC8I GCNNGC 2 cut(s) 207, 472
BstDEI CTNAG 3 cut(s) 14, 53, 579
BstDSI CCRYGG 1 cut(s) 629
BstEII GGTNACC 1 cut(s) 84
BstF5I GGATG 1 cut(s) 561
BstFNI CGCG 1 cut(s) 9
BstKTI GATC 4 cut(s) 13, 250, 298, 618
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 4 cut(s) 10, 247, 295, 615
BstMWI GCNNNNNNNGC 2 cut(s) 57, 148
BstNI CCWGG 2 cut(s) 122, 187
BstNSI RCATGY 1 cut(s) 539
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 120, 185, 239
BstUI CGCG 1 cut(s) 9
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 2 cut(s) 247, 615
BstXI CCANNNNNNTGG 1 cut(s) 519
BstYI RGATCY 2 cut(s) 247, 615
Bsu15I ATCGAT 1 cut(s) 602
Bsu36I CCTNAGG 1 cut(s) 53
BsuRI GGCC 1 cut(s) 19
BsuTUI ATCGAT 1 cut(s) 602
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 207, 472
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 633
ClaI ATCGAT 1 cut(s) 602
Csp6I GTAC 3 cut(s) 301, 463, 533
CspAI ACCGGT 1 cut(s) 70
CviAII CATG 6 cut(s) 42, 79, 127, 475, 536, 630
CviJI RGCY 7 cut(s) 19, 60, 190, 209, 259, 578, 639
CviKI_1 RGCY 7 cut(s) 19, 60, 190, 209, 259, 578, 639
CviQI GTAC 3 cut(s) 301, 463, 533
DdeI CTNAG 3 cut(s) 14, 53, 579
DpnI GATC 4 cut(s) 12, 249, 297, 617
DpnII GATC 4 cut(s) 10, 247, 295, 615
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 629
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 633
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 558
Eco81I CCTNAGG 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 120, 185
EcoT14I CCWWGG 1 cut(s) 629
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 629
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 6 cut(s) 45, 82, 130, 478, 539, 633
FaqI GGGAC 1 cut(s) 87
FatI CATG 6 cut(s) 41, 78, 126, 474, 535, 629
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 7
FspBI CTAG 2 cut(s) 566, 585
GluI GCNGC 1 cut(s) 7
HaeIII GGCC 1 cut(s) 19
HapII CCGG 2 cut(s) 71, 240
Hin1II CATG 6 cut(s) 45, 82, 130, 478, 539, 633
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 8 cut(s) 92, 146, 236, 318, 434, 446, 599, 604
HpaII CCGG 2 cut(s) 71, 240
HphI GGTGA 3 cut(s) 78, 244, 535
Hpy188I TCNGA 7 cut(s) 247, 268, 317, 378, 486, 562, 582
Hpy188III TCNNGA 5 cut(s) 170, 212, 228, 443, 548
HpyAV CCTTC 1 cut(s) 523
HpyCH4V TGCA 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 148
HpyF3I CTNAG 3 cut(s) 14, 53, 579
Hsp92II CATG 6 cut(s) 45, 82, 130, 478, 539, 633
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 4 cut(s) 10, 247, 295, 615
LmnI GCTCC 3 cut(s) 57, 65, 644
LweI GCATC 2 cut(s) 282, 457
MaeI CTAG 2 cut(s) 566, 585
MaeIII GTNAC 1 cut(s) 84
MalI GATC 4 cut(s) 12, 249, 297, 617
MboI GATC 4 cut(s) 10, 247, 295, 615
MboII GAAGA 5 cut(s) 158, 328, 368, 538, 564
MflI RGATCY 2 cut(s) 247, 615
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 4 cut(s) 65, 281, 327, 458
MlyI GAGTC 2 cut(s) 86, 140
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 48, 106, 115, 186, 208, 332, 492, 629
MroXI GAANNNNTTC 3 cut(s) 235, 438, 603
MseI TTAA 1 cut(s) 624
MspI CCGG 2 cut(s) 71, 240
MspR9I CCNGG 3 cut(s) 122, 187, 241
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 2 cut(s) 122, 187
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 57, 148
NciI CCSGG 1 cut(s) 241
NcoI CCATGG 1 cut(s) 629
NdeII GATC 4 cut(s) 10, 247, 295, 615
NlaIII CATG 6 cut(s) 45, 82, 130, 478, 539, 633
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 1 cut(s) 84
NspI RCATGY 1 cut(s) 539
PceI AGGCCT 1 cut(s) 19
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 3 cut(s) 235, 438, 603
PfeI GAWTC 6 cut(s) 236, 318, 434, 446, 599, 604
PinAI ACCGGT 1 cut(s) 70
PkrI GCNGC 1 cut(s) 8
PleI GAGTC 2 cut(s) 86, 140
PpsI GAGTC 2 cut(s) 86, 140
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 120, 185
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 120, 185
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 1 cut(s) 633
PsuI RGATCY 2 cut(s) 247, 615
RsaI GTAC 3 cut(s) 302, 464, 534
RsaNI GTAC 3 cut(s) 301, 463, 533
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 4 cut(s) 10, 247, 295, 615
Sau96I GGNCC 1 cut(s) 633
SchI GAGTC 2 cut(s) 86, 140
ScrFI CCNGG 3 cut(s) 122, 187, 241
SduI GDGCHC 1 cut(s) 62
SetI ASST 7 cut(s) 62, 91, 211, 261, 534, 580, 641
SfaNI GCATC 2 cut(s) 282, 457
SinI GGWCC 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SpeI ACTAGT 2 cut(s) 565, 584
Sse9I AATT 4 cut(s) 65, 281, 327, 458
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 7, 116
SspMI CTAG 2 cut(s) 566, 585
SstI GAGCTC 1 cut(s) 62
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 3 cut(s) 120, 185, 239
StyI CCWWGG 1 cut(s) 629
TaqI TCGA 3 cut(s) 197, 497, 602
TasI AATT 4 cut(s) 65, 281, 327, 458
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 6 cut(s) 236, 318, 434, 446, 599, 604
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 84
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 2 cut(s) 447, 612
TspRI CASTG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 633
XapI RAATTY 2 cut(s) 281, 327
XceI RCATGY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmnI GAANNNNTTC 3 cut(s) 235, 438, 603
XspI CTAG 2 cut(s) 566, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.