Prupe.3G203400_v2.0.a1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0022498
Physical Location & Seq
Forward (+)
21092840 .. 21094061
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G203400.1

Sequence Viewer

Length: 612 bp
ATGATAGCAAAAGGAAACTACCTGGTTGGTCCAGTGAAGTTTCAAGGGCCCTGCAAAGCTCCGGTTAGTGTTCGAGTTGAAGGAGCCCTGCAGGCTCTTGTAGAGCCAGAAAAGCTCAAGTTAGAAGATGGTTGGGTCATTTTCCAAAATATTGATGGGGTAACAGTCTCAGGAGATGGCACTTTTGATGGCCAAGGATCAATAGCGCCTAGTCAATTTTCTTATGTCGAGTCGCTACCGTCACTCCTCTCTTCGATTCTGCTGTGCTGGCCTCGTTGTTGTCTCTCTCATATTCTGGGTGTTGGTTTTGTCATTCACTTTCTCCTCGGTGCGTCAGCATTTCTGGTGGTTGTCTTAGTATGGTGGTTTCAGTGGGTCTGCTGCTGCTATTGTGCCGACAGTCACCAAGTCAACGATGACTATGGTGCAAGCCGCTTCGTTGGGGTCGGCGCTCACGGTGGAGAATATTGTCCAAATGGTCAGTGCCAAGCAAAAATCCTGTCAAAAGTTAAGATAAACAATGTGAGGTTTAAGAACATTAGGGGCACTGCTGCTGATCCTGTTGTTGTGAAGCTTGCTTGCAGCAAAGGCATTCCACGCCAGAATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

204

Amino Acids

21.92

Weight (kDa)

8.28

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022498)

Species Orthologous Gene IDs
prunus_persica Prupe.3G203400_v2.0.a1 Prupe.3G203500_v2.0.a1 Prupe.3G203700_v2.0.a1 Prupe.3G203800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 433
AclWI GGATC 2 cut(s) 205, 551
AcoI YGGCCR 1 cut(s) 190
AgsI TTSAA 2 cut(s) 44, 80
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 3 cut(s) 59, 115, 574
AluI AGCT 3 cut(s) 59, 115, 574
Alw26I GTCTC 2 cut(s) 172, 287
AlwI GGATC 2 cut(s) 205, 551
AoxI GGCC 3 cut(s) 47, 190, 269
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 4 cut(s) 381, 384, 551, 582
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 2 cut(s) 208, 452
AspS9I GGNCC 3 cut(s) 29, 47, 48
AsuHPI GGTGA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 29
BaeGI GKGCMC 2 cut(s) 51, 548
BalI TGGCCA 1 cut(s) 192
BanII GRGCYC 2 cut(s) 51, 88
BbvI GCAGC 4 cut(s) 368, 371, 538, 594
BccI CCATC 4 cut(s) 122, 149, 170, 182
BciT130I CCWGG 1 cut(s) 23
BcoDI GTCTC 2 cut(s) 172, 287
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 89
BfoI RGCGCY 2 cut(s) 209, 453
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 5 cut(s) 382, 385, 433, 552, 583
BlsI GCNGC 5 cut(s) 383, 386, 434, 553, 584
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 1 cut(s) 29
BmgT120I GGNCC 3 cut(s) 29, 47, 48
BmiI GGNNCC 2 cut(s) 49, 85
BmrFI CCNGG 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 193, 325
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bse1I ACTGG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 193, 325
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 2 cut(s) 236, 314
BseSI GKGCMC 2 cut(s) 51, 548
BseXI GCAGC 4 cut(s) 368, 371, 538, 594
BshFI GGCC 3 cut(s) 49, 192, 271
BsiSI CCGG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 172, 287
BsmI GAATGC 1 cut(s) 591
BsnI GGCC 3 cut(s) 49, 192, 271
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 3 cut(s) 51, 88, 548
Bsp143I GATC 2 cut(s) 197, 556
BspACI CCGC 1 cut(s) 433
BspANI GGCC 3 cut(s) 49, 192, 271
BspCNI CTCAG 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 49, 85
BspMAI CTGCAG 1 cut(s) 93
BspPI GGATC 2 cut(s) 205, 551
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 193, 325
BssMI GATC 2 cut(s) 197, 556
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 4 cut(s) 166, 240, 401, 458
Bst6I CTCTTC 1 cut(s) 256
BstC8I GCNNGC 5 cut(s) 93, 269, 430, 576, 580
BstDEI CTNAG 2 cut(s) 169, 355
BstH2I RGCGCY 2 cut(s) 209, 453
BstHHI GCGC 2 cut(s) 208, 452
BstKTI GATC 2 cut(s) 200, 559
BstMAI GTCTC 2 cut(s) 172, 287
BstMBI GATC 2 cut(s) 197, 556
BstMWI GCNNNNNNNGC 5 cut(s) 92, 112, 268, 588, 597
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 89
BstSLI GKGCMC 2 cut(s) 51, 548
BstV1I GCAGC 4 cut(s) 368, 371, 538, 594
BsuRI GGCC 3 cut(s) 49, 192, 271
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 4 cut(s) 39, 377, 488, 546
Cac8I GCNNGC 5 cut(s) 93, 269, 430, 576, 580
CfoI GCGC 2 cut(s) 208, 452
Cfr13I GGNCC 3 cut(s) 29, 47, 48
CseI GACGC 1 cut(s) 321
CsiI ACCWGGT 1 cut(s) 21
DdeI CTNAG 2 cut(s) 169, 355
DpnI GATC 2 cut(s) 199, 558
DpnII GATC 2 cut(s) 197, 556
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 193
Eco24I GRGCYC 2 cut(s) 51, 88
Eco47I GGWCC 1 cut(s) 29
EcoO109I RGGNCCY 2 cut(s) 47, 48
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 193
EcoT38I GRGCYC 2 cut(s) 51, 88
ErhI CCWWGG 1 cut(s) 193
FaiI YATR 5 cut(s) 225, 291, 361, 423, 610
Fnu4HI GCNGC 5 cut(s) 382, 385, 433, 552, 583
FriOI GRGCYC 2 cut(s) 51, 88
Fsp4HI GCNGC 5 cut(s) 382, 385, 433, 552, 583
FspBI CTAG 1 cut(s) 210
GlaI GCGC 2 cut(s) 207, 451
GluI GCNGC 5 cut(s) 382, 385, 433, 552, 583
HaeII RGCGCY 2 cut(s) 209, 453
HaeIII GGCC 3 cut(s) 49, 192, 271
HapII CCGG 1 cut(s) 62
HgaI GACGC 1 cut(s) 321
HhaI GCGC 2 cut(s) 208, 452
Hin6I GCGC 2 cut(s) 206, 450
HinP1I GCGC 2 cut(s) 206, 450
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 230, 256
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 1 cut(s) 412
Hpy188III TCNNGA 1 cut(s) 171
Hpy8I GTNNAC 1 cut(s) 412
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 4 cut(s) 166, 240, 401, 458
HpyCH4V TGCA 4 cut(s) 54, 91, 428, 582
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 112, 268, 588, 597
HpyF3I CTNAG 2 cut(s) 169, 355
HspAI GCGC 2 cut(s) 206, 450
Kzo9I GATC 2 cut(s) 197, 556
LmnI GCTCC 2 cut(s) 64, 83
Lsp1109I GCAGC 4 cut(s) 368, 371, 538, 594
MabI ACCWGGT 1 cut(s) 21
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 3 cut(s) 160, 240, 401
MalI GATC 2 cut(s) 199, 558
MboI GATC 2 cut(s) 197, 556
MboII GAAGA 2 cut(s) 137, 243
MhlI GDGCHC 3 cut(s) 51, 88, 548
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 1 cut(s) 215
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 4 cut(s) 257, 282, 335, 519
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 2 cut(s) 510, 531
Msp20I TGGCCA 1 cut(s) 192
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 23
Mva1269I GAATGC 1 cut(s) 591
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 92, 112, 268, 588, 597
NdeII GATC 2 cut(s) 197, 556
NlaIV GGNNCC 2 cut(s) 49, 85
NmuCI GTSAC 2 cut(s) 240, 401
PctI GAATGC 1 cut(s) 591
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 5 cut(s) 383, 386, 434, 553, 584
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 49, 85
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 3 cut(s) 29, 47, 48
PstI CTGCAG 1 cut(s) 93
SaqAI TTAA 2 cut(s) 510, 531
SatI GCNGC 5 cut(s) 382, 385, 433, 552, 583
Sau3AI GATC 2 cut(s) 197, 556
Sau96I GGNCC 3 cut(s) 29, 47, 48
SbfI CCTGCAGG 1 cut(s) 93
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 23
SdaI CCTGCAGG 1 cut(s) 93
SduI GDGCHC 3 cut(s) 51, 88, 548
SetI ASST 5 cut(s) 24, 61, 117, 530, 576
SexAI ACCWGGT 1 cut(s) 21
SfcI CTRYAG 1 cut(s) 89
SinI GGWCC 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse8387I CCTGCAGG 1 cut(s) 93
Sse9I AATT 1 cut(s) 215
SsiI CCGC 1 cut(s) 433
SspI AATATT 2 cut(s) 151, 467
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 4 cut(s) 166, 240, 401, 458
TaqI TCGA 3 cut(s) 73, 228, 254
TasI AATT 1 cut(s) 215
TauI GCSGC 1 cut(s) 435
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 2 cut(s) 510, 531
Tru9I TTAA 2 cut(s) 510, 531
TscAI CASTG 4 cut(s) 39, 377, 488, 553
TseFI GTSAC 2 cut(s) 240, 401
TseI GCWGC 4 cut(s) 381, 384, 551, 582
Tsp45I GTSAC 2 cut(s) 240, 401
TspRI CASTG 4 cut(s) 39, 377, 488, 553
VpaK11BI GGWCC 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.