Prupe.3G203700_v2.0.a1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0022498
Physical Location & Seq
Forward (+)
21162478 .. 21164963
2486 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G203700.1

Sequence Viewer

Length: 504 bp
ATGAATCTTAAAATCAAGTTGTTTGACAGAATCAACCTCAGCGGCTTTGGTGGGTTAAGCAGCAAGTTTGGCAGCAAGGCTATTAATAGTACTTGGAAAGAAGCAAGCGCATCCACAACTTCAAACACGGTTATGATAGCAAAAGGAAACTACCTGGTTGGTCCAGTGAAGTTTCAAGGGCCCTGCAAAGCTCCGGTTAGTGTTCGAGTTGAAGGAGCCCTGCAGGCTCTTGTAGAGCCAGAAAAGCTCAAGTTAGAAGATGGTTGGGTCATTTTCCAAAATATTGATGGGGTAACAGTCTCAGGAGATGGCACTTTTGATGGCCAAGGATCAATAGCGAAAATGCAACTCACTGCCTATAGTAAGTTTTTTAAATTTGAAGTGGACCTTCAACATGTGATCATCACCACCCCTGGTGAAAGTCTAACCACAGATGGAATCCACATCCCCGTTGATGTATATTTTCGAACACCTGAAAAAGAGTATCCAAAAATTACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

168

Amino Acids

18.21

Weight (kDa)

7.79

Isoelectric Point (pI)

21.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022498)

Species Orthologous Gene IDs
prunus_persica Prupe.3G203400_v2.0.a1 Prupe.3G203500_v2.0.a1 Prupe.3G203700_v2.0.a1 Prupe.3G203800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 374
AfaI GTAC 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 123, 176, 212, 380, 392
AjnI CCWGG 2 cut(s) 153, 412
AluBI AGCT 2 cut(s) 191, 247
AluI AGCT 2 cut(s) 191, 247
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 337
AoxI GGCC 2 cut(s) 179, 322
ApaI GGGCCC 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 60, 72
ApoI RAATTY 1 cut(s) 374
AseI ATTAAT 1 cut(s) 84
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 4 cut(s) 161, 179, 180, 385
AsuHPI GGTGA 2 cut(s) 397, 428
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 2 cut(s) 161, 385
BaeGI GKGCMC 1 cut(s) 183
BalI TGGCCA 1 cut(s) 324
BanII GRGCYC 2 cut(s) 183, 220
BbvCI CCTCAGC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 72, 84
BccI CCATC 5 cut(s) 254, 281, 302, 314, 428
BciT130I CCWGG 2 cut(s) 155, 414
BciVI GTATCC 1 cut(s) 495
BclI TGATCA 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 304
BfmI CTRYAG 2 cut(s) 221, 358
BfuI GTATCC 1 cut(s) 495
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 3 cut(s) 43, 61, 73
BlsI GCNGC 3 cut(s) 44, 62, 74
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 155, 414
Bme18I GGWCC 2 cut(s) 161, 385
BmgT120I GGNCC 4 cut(s) 161, 179, 180, 385
BmiI GGNNCC 2 cut(s) 181, 217
BmrFI CCNGG 2 cut(s) 155, 414
BmsI GCATC 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 38
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 325, 412
BsaWI WCCGGW 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 164
BseBI CCWGG 2 cut(s) 155, 414
BseDI CCNNGG 2 cut(s) 325, 412
BseGI GGATG 2 cut(s) 110, 444
BseMII CTCAG 2 cut(s) 52, 315
BseNI ACTGG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 2 cut(s) 72, 84
BshFI GGCC 2 cut(s) 181, 324
BsiSI CCGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 2 cut(s) 181, 324
Bsp119I TTCGAA 1 cut(s) 466
Bsp120I GGGCCC 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 183, 220
Bsp143I GATC 2 cut(s) 329, 399
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 181, 324
BspCNI CTCAG 2 cut(s) 51, 314
BspLI GGNNCC 2 cut(s) 181, 217
BspMAI CTGCAG 1 cut(s) 225
BspPI GGATC 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 466
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 325, 412
BssMI GATC 2 cut(s) 329, 399
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 2 cut(s) 155, 414
Bst4CI ACNGT 2 cut(s) 130, 298
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 106, 225
BstDEI CTNAG 2 cut(s) 38, 301
BstF5I GGATG 2 cut(s) 110, 444
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 2 cut(s) 332, 402
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 2 cut(s) 329, 399
BstMWI GCNNNNNNNGC 3 cut(s) 69, 224, 244
BstNI CCWGG 2 cut(s) 155, 414
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 153, 412
BstSFI CTRYAG 2 cut(s) 221, 358
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 72, 84
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 2 cut(s) 181, 324
BtsCI GGATG 2 cut(s) 110, 444
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 171, 351
Cac8I GCNNGC 2 cut(s) 106, 225
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 4 cut(s) 161, 179, 180, 385
CsiI ACCWGGT 1 cut(s) 153
Csp6I GTAC 1 cut(s) 90
CviAII CATG 1 cut(s) 395
CviJI RGCY 9 cut(s) 45, 80, 181, 191, 218, 227, 238, 247, 324
CviKI_1 RGCY 9 cut(s) 45, 80, 181, 191, 218, 227, 238, 247, 324
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 2 cut(s) 38, 301
DpnI GATC 2 cut(s) 331, 401
DpnII GATC 2 cut(s) 329, 399
DraI TTTAAA 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 325
Eco24I GRGCYC 2 cut(s) 183, 220
Eco47I GGWCC 2 cut(s) 161, 385
EcoO109I RGGNCCY 2 cut(s) 179, 180
EcoRII CCWGG 2 cut(s) 153, 412
EcoT14I CCWWGG 1 cut(s) 325
EcoT38I GRGCYC 2 cut(s) 183, 220
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 1 cut(s) 398
FaiI YATR 4 cut(s) 134, 360, 396, 460
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FatI CATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 43, 61, 73
FokI GGATG 2 cut(s) 97, 431
FriOI GRGCYC 2 cut(s) 183, 220
Fsp4HI GCNGC 3 cut(s) 43, 61, 73
GlaI GCGC 1 cut(s) 109
GluI GCNGC 3 cut(s) 43, 61, 73
HaeIII GGCC 2 cut(s) 181, 324
HapII CCGG 1 cut(s) 194
HhaI GCGC 1 cut(s) 110
Hin1II CATG 1 cut(s) 398
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 3 cut(s) 4, 30, 438
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 397, 428
Hpy166II GTNNAC 1 cut(s) 385
Hpy188III TCNNGA 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 385
HpyAV CCTTC 2 cut(s) 206, 398
HpyCH4III ACNGT 2 cut(s) 130, 298
HpyCH4V TGCA 3 cut(s) 186, 223, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 224, 244
HpyF3I CTNAG 2 cut(s) 38, 301
Hsp92II CATG 1 cut(s) 398
HspAI GCGC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 2 cut(s) 329, 399
LmnI GCTCC 2 cut(s) 196, 215
Lsp1109I GCAGC 2 cut(s) 72, 84
LweI GCATC 1 cut(s) 119
MabI ACCWGGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 331, 401
MboI GATC 2 cut(s) 329, 399
MboII GAAGA 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 183, 220
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 3 cut(s) 374, 492, 499
MluNI TGGCCA 1 cut(s) 324
MnlI CCTC 1 cut(s) 47
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MseI TTAA 5 cut(s) 9, 56, 84, 372, 502
MslI CAYNNNNRTG 1 cut(s) 131
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 155, 414
MvaI CCWGG 2 cut(s) 155, 414
MwoI GCNNNNNNNGC 3 cut(s) 69, 224, 244
NdeII GATC 2 cut(s) 329, 399
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 2 cut(s) 181, 217
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 1 cut(s) 466
PciI ACATGT 1 cut(s) 394
PfeI GAWTC 3 cut(s) 4, 30, 438
PkrI GCNGC 3 cut(s) 44, 62, 74
PscI ACATGT 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 153, 412
PspGI CCWGG 2 cut(s) 153, 412
PspN4I GGNNCC 2 cut(s) 181, 217
PspOMI GGGCCC 1 cut(s) 179
PspPI GGNCC 4 cut(s) 161, 179, 180, 385
PstI CTGCAG 1 cut(s) 225
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 5 cut(s) 9, 56, 84, 372, 502
SatI GCNGC 3 cut(s) 43, 61, 73
Sau3AI GATC 2 cut(s) 329, 399
Sau96I GGNCC 4 cut(s) 161, 179, 180, 385
SbfI CCTGCAGG 1 cut(s) 225
ScaI AGTACT 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 155, 414
SdaI CCTGCAGG 1 cut(s) 225
SduI GDGCHC 2 cut(s) 183, 220
SetI ASST 6 cut(s) 39, 156, 193, 249, 390, 475
SexAI ACCWGGT 1 cut(s) 153
SfaNI GCATC 1 cut(s) 119
SfcI CTRYAG 2 cut(s) 221, 358
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 2 cut(s) 161, 385
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse8387I CCTGCAGG 1 cut(s) 225
Sse9I AATT 3 cut(s) 374, 492, 499
SsiI CCGC 1 cut(s) 42
SspI AATATT 1 cut(s) 283
StyD4I CCNGG 2 cut(s) 153, 412
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 2 cut(s) 130, 298
TaqI TCGA 2 cut(s) 205, 466
TasI AATT 3 cut(s) 374, 492, 499
TatI WGTACW 1 cut(s) 89
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 4, 30, 438
Tru1I TTAA 5 cut(s) 9, 56, 84, 372, 502
Tru9I TTAA 5 cut(s) 9, 56, 84, 372, 502
TscAI CASTG 2 cut(s) 171, 358
TseI GCWGC 2 cut(s) 60, 72
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 171, 358
VpaK11BI GGWCC 2 cut(s) 161, 385
VspI ATTAAT 1 cut(s) 84
XapI RAATTY 1 cut(s) 374
XceI RCATGY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.