Prupe.4G283600_v2.0.a1

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0021223
Physical Location & Seq
Reverse (-)
24419175 .. 24422519
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G283600.1

Sequence Viewer

Length: 456 bp
ATGATTGTGTTTCCTTTCTATTTATGCTTTTCATTTAAGTTTAGAAATTCGTTAGAAAAAAGAAGGAAAAACTCATTTTATGCATCCACGAGGCCCATTCGAACTTTAGACCCTTGGAATAGGAGAAATGCCAATGCGGTTATGGAGAAGGCTGCGATTTTGATTGCAAGTACCAACACTCCTGCCAGGTACAAGGAGACATGGAGGAATATTTTGAACAAATACAAACAACGTATTATTTTAGATGCTGGGCAACTGCGGGAGAAGCATCCAAATTGTATACCAGTGACTGTGGACAAGGATGCAAGGAGTGACATTCATGACATTGACACTAAGAAGTGCCTTGGATGTCTACAATCAATGAGGAAAATAAGGATGAAGATGGATTTCTTCACATCACCTACAGTGGAGAAGAGAGTGTGTGGATCCAATGAGGAACAGCAAGAATTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.82

Weight (kDa)

9.85

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 280, 352
AciI CCGC 2 cut(s) 137, 259
AclWI GGATC 2 cut(s) 420, 433
AcsI RAATTY 2 cut(s) 46, 446
AfaI GTAC 2 cut(s) 172, 191
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 2 cut(s) 420, 433
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 2 cut(s) 46, 446
AspS9I GGNCC 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 390
AsuII TTCGAA 1 cut(s) 100
BamHI GGATCC 1 cut(s) 425
BauI CACGAG 1 cut(s) 88
BbvI GCAGC 1 cut(s) 139
BccI CCATC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 4 cut(s) 92, 235, 277, 292
Bpu14I TTCGAA 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 113, 343
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bse1I ACTGG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 113, 343
BseGI GGATG 5 cut(s) 83, 268, 307, 353, 381
BseNI ACTGG 1 cut(s) 284
BseXI GCAGC 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 248
BshFI GGCC 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 100
Bsp143I GATC 1 cut(s) 425
BspACI CCGC 2 cut(s) 137, 259
BspANI GGCC 1 cut(s) 94
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 2 cut(s) 420, 433
BspT104I TTCGAA 1 cut(s) 100
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 113, 343
BssMI GATC 1 cut(s) 425
BssNAI GTATAC 1 cut(s) 281
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 113, 343
Bst1107I GTATAC 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 2 cut(s) 292, 406
Bst6I CTCTTC 1 cut(s) 407
BstBI TTCGAA 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 5 cut(s) 83, 268, 307, 353, 381
BstKTI GATC 1 cut(s) 428
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 425
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BstZ17I GTATAC 1 cut(s) 281
BsuRI GGCC 1 cut(s) 94
BtsCI GGATG 5 cut(s) 83, 268, 307, 353, 381
BtsIMutI CAGTG 2 cut(s) 291, 411
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 93
Csp6I GTAC 2 cut(s) 171, 190
CviAII CATG 2 cut(s) 201, 320
CviJI RGCY 2 cut(s) 94, 152
CviKI_1 RGCY 2 cut(s) 94, 152
CviQI GTAC 2 cut(s) 171, 190
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 427
DpnII GATC 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
Eco130I CCWWGG 2 cut(s) 113, 343
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 2 cut(s) 113, 343
EcoT22I ATGCAT 1 cut(s) 85
ErhI CCWWGG 2 cut(s) 113, 343
FaeI CATG 2 cut(s) 204, 323
FaiI YATR 6 cut(s) 25, 81, 143, 202, 281, 321
FatI CATG 2 cut(s) 200, 319
FauI CCCGC 1 cut(s) 252
FblI GTMKAC 2 cut(s) 280, 352
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 5 cut(s) 70, 255, 314, 360, 388
Fsp4HI GCNGC 1 cut(s) 153
GluI GCNGC 1 cut(s) 153
GsaI CCCAGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 94
Hin1II CATG 2 cut(s) 204, 323
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 3 cut(s) 281, 295, 353
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 3 cut(s) 281, 295, 353
HpyAV CCTTC 2 cut(s) 57, 142
HpyCH4III ACNGT 2 cut(s) 292, 406
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 3 cut(s) 83, 167, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 2 cut(s) 204, 323
Kzo9I GATC 1 cut(s) 425
LpnPI CCDG 5 cut(s) 172, 195, 199, 234, 297
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 4 cut(s) 92, 235, 277, 292
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 2 cut(s) 286, 311
MalI GATC 1 cut(s) 427
MboI GATC 1 cut(s) 425
MboII GAAGA 3 cut(s) 382, 391, 424
MflI RGATCY 1 cut(s) 425
MluCI AATT 3 cut(s) 46, 274, 446
MnlI CCTC 4 cut(s) 84, 198, 357, 427
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 2 cut(s) 36, 454
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
MwoI GCNNNNNNNGC 1 cut(s) 265
NdeII GATC 1 cut(s) 425
NlaIII CATG 2 cut(s) 204, 323
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 286, 311
NsiI ATGCAT 1 cut(s) 85
NspV TTCGAA 1 cut(s) 100
PagI TCATGA 1 cut(s) 319
PkrI GCNGC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 425
RsaI GTAC 2 cut(s) 172, 191
RsaNI GTAC 2 cut(s) 171, 190
SaqAI TTAA 2 cut(s) 36, 454
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 425
Sau96I GGNCC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 187
SetI ASST 3 cut(s) 191, 235, 403
SfaNI GCATC 4 cut(s) 92, 235, 277, 292
SfcI CTRYAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 100
Sse9I AATT 3 cut(s) 46, 274, 446
SsiI CCGC 2 cut(s) 137, 259
SspI AATATT 1 cut(s) 211
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 2 cut(s) 113, 343
TaaI ACNGT 2 cut(s) 292, 406
TaiI ACGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 100
TasI AATT 3 cut(s) 46, 274, 446
Tru1I TTAA 2 cut(s) 36, 454
Tru9I TTAA 2 cut(s) 36, 454
TscAI CASTG 2 cut(s) 291, 411
TseFI GTSAC 2 cut(s) 286, 311
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 286, 311
TspDTI ATGAA 3 cut(s) 21, 308, 392
TspRI CASTG 2 cut(s) 291, 411
XapI RAATTY 2 cut(s) 46, 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmiI GTMKAC 2 cut(s) 280, 352
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.