Prupe.5G033500_v2.0.a1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0024550
Physical Location & Seq
Forward (+)
3792997 .. 3796966
3970 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G033500.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 516 bp
ATGGAATCGTTTAAGAGTAAGGTGGAGGAGGAGTACAACCCGTTCAACATGTCGATGAATAAATCACGCTTGTCGAGACTCATTCGAACGGAGTTGGAGGATATGCAATCCAATCCCTCTGCCGATTTCATGTGCCTCTCGCTCGAGTCGAACCTGCGGGACTTGCAATTTGCAATCAGAGGCCCTAATGGAACTGAATTCGAGGGTGGGATTTATCATGGCCAGATCAAGATTCCGGAGGAATACCCACACAAGCCTCCTATAATCACGTTGTTGTCGGAAAATGGTCGCTTCAAAACCCAAACCGAGATCGATTATTTCAGCAATTTTTTTGGGGGTCCACGCAATAAATTGCGACATGCTTTACTTAGACTTATCGAGTTATTGCCCACCTACCCAGATGGTGCGTTGGGTTCAGTAGAATACGATAAGGAAGAAAGGCGTGTCCTGGCAATCAAATCTCGTTTAGCAGCCCCAAGATATGGCACTGATGAACGGCAGAATTTAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 ko05012 map04120 map04141 map05012
Pfam Domains
Protein Families
UQ_con

Protein Analysis

172

Amino Acids

19.75

Weight (kDa)

5.98

Isoelectric Point (pI)

50.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024550)

Species Orthologous Gene IDs
prunus_persica Prupe.5G032100_v2.0.a1 Prupe.5G033500_v2.0.a1 Prupe.5G033600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 482
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 1 cut(s) 157
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 2 cut(s) 197, 502
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 2 cut(s) 46, 295
AjnI CCWGG 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 70
Ama87I CYCGRG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 181, 220
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 2 cut(s) 197, 502
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AseI ATTAAT 1 cut(s) 510
AspS9I GGNCC 2 cut(s) 182, 338
AsuII TTCGAA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 1 cut(s) 338
BalI TGGCCA 1 cut(s) 222
BbvI GCAGC 1 cut(s) 482
BccI CCATC 1 cut(s) 395
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 70
BfuAI ACCTGC 1 cut(s) 162
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
Bme1390I CCNGG 1 cut(s) 449
Bme18I GGWCC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 182, 338
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 449
Bpu14I TTCGAA 1 cut(s) 85
Bsa29I ATCGAT 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 1 cut(s) 482
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 1 cut(s) 449
BseCI ATCGAT 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 482
BseRI GAGGAG 2 cut(s) 41, 44
BseXI GCAGC 1 cut(s) 482
BshFI GGCC 2 cut(s) 183, 222
BshVI ATCGAT 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 2 cut(s) 183, 222
BsoBI CYCGRG 1 cut(s) 143
Bsp119I TTCGAA 1 cut(s) 85
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 2 cut(s) 225, 309
BspACI CCGC 1 cut(s) 157
BspANI GGCC 2 cut(s) 183, 222
BspDI ATCGAT 1 cut(s) 312
BspEI TCCGGA 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 339
BspMI ACCTGC 1 cut(s) 162
BspT104I TTCGAA 1 cut(s) 85
BssMI GATC 2 cut(s) 225, 309
Bst2UI CCWGG 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 368
BstKTI GATC 2 cut(s) 228, 312
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 2 cut(s) 225, 309
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 52, 362
BstSCI CCNGG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 482
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 2 cut(s) 183, 222
BsuTUI ATCGAT 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 486
BveI ACCTGC 1 cut(s) 162
Cfr13I GGNCC 2 cut(s) 182, 338
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 34
CviAII CATG 4 cut(s) 49, 130, 218, 359
CviJI RGCY 4 cut(s) 183, 222, 256, 473
CviKI_1 RGCY 4 cut(s) 183, 222, 256, 473
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 1 cut(s) 368
DpnI GATC 2 cut(s) 227, 311
DpnII GATC 2 cut(s) 225, 309
EaeI YGGCCR 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 447
FaeI CATG 4 cut(s) 52, 133, 221, 362
FaiI YATR 7 cut(s) 50, 104, 131, 219, 263, 360, 483
FaqI GGGAC 1 cut(s) 173
FatI CATG 4 cut(s) 48, 129, 217, 358
FauI CCCGC 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 471
GluI GCNGC 1 cut(s) 471
HaeIII GGCC 2 cut(s) 183, 222
HapII CCGG 1 cut(s) 236
Hin1II CATG 4 cut(s) 52, 133, 221, 362
HinfI GANTC 4 cut(s) 5, 78, 146, 232
HpaII CCGG 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 2 cut(s) 179, 280
Hpy188III TCNNGA 3 cut(s) 75, 229, 236
Hpy8I GTNNAC 1 cut(s) 341
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 3 cut(s) 106, 166, 173
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 4 cut(s) 52, 133, 221, 362
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 2 cut(s) 225, 309
LpnPI CCDG 6 cut(s) 167, 236, 249, 411, 434, 461
Lsp1109I GCAGC 1 cut(s) 482
MaeII ACGT 1 cut(s) 269
MalI GATC 2 cut(s) 227, 311
MboI GATC 2 cut(s) 225, 309
MboII GAAGA 1 cut(s) 446
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 7 cut(s) 167, 197, 325, 350, 502, 507, 511
MluNI TGGCCA 1 cut(s) 222
MlyI GAGTC 2 cut(s) 72, 155
MmeI TCCRAC 2 cut(s) 75, 258
MnlI CCTC 9 cut(s) 19, 22, 91, 127, 146, 173, 196, 232, 267
Mox20I TGGCCA 1 cut(s) 222
MroI TCCGGA 1 cut(s) 235
MscI TGGCCA 1 cut(s) 222
MseI TTAA 3 cut(s) 12, 506, 510
MslI CAYNNNNRTG 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 2 cut(s) 225, 309
NlaIII CATG 4 cut(s) 52, 133, 221, 362
NlaIV GGNNCC 1 cut(s) 339
NspI RCATGY 2 cut(s) 52, 362
NspV TTCGAA 1 cut(s) 85
PacI TTAATTAA 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 143
PciI ACATGT 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PfeI GAWTC 2 cut(s) 5, 232
PflMI CCANNNNNTGG 1 cut(s) 482
PkrI GCNGC 1 cut(s) 472
PleI GAGTC 2 cut(s) 72, 154
PpsI GAGTC 2 cut(s) 72, 154
PscI ACATGT 1 cut(s) 48
PshBI ATTAAT 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 182, 338
PspXI VCTCGAGB 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 26, 58
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 53
SaqAI TTAA 3 cut(s) 12, 506, 510
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 2 cut(s) 225, 309
Sau96I GGNCC 2 cut(s) 182, 338
SchI GAGTC 2 cut(s) 72, 155
ScrFI CCNGG 1 cut(s) 449
SetI ASST 4 cut(s) 24, 156, 272, 395
Sfr274I CTCGAG 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 85
SinI GGWCC 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 7 cut(s) 167, 197, 325, 350, 502, 507, 511
SsiI CCGC 1 cut(s) 157
StyD4I CCNGG 1 cut(s) 447
TaiI ACGT 1 cut(s) 272
TaqI TCGA 8 cut(s) 53, 74, 85, 144, 149, 201, 312, 378
TasI AATT 7 cut(s) 167, 197, 325, 350, 502, 507, 511
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 2 cut(s) 5, 232
Tru1I TTAA 3 cut(s) 12, 506, 510
Tru9I TTAA 3 cut(s) 12, 506, 510
TscAI CASTG 1 cut(s) 493
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 71, 118, 507
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 1 cut(s) 493
Van91I CCANNNNNTGG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 338
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 2 cut(s) 197, 502
XceI RCATGY 2 cut(s) 52, 362
XhoI CTCGAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.