Prupe.5G230200_v2.0.a1

alpha-dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0019156
Physical Location & Seq
Forward (+)
17589567 .. 17593339
3773 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G230200.1

Sequence Viewer

Length: 1902 bp
ATGGCCTTCTTTCTCTCTTCTTGTTCTACCTTCGTTCATCCCCAACTCCAGCCTATTGTAGCCAAAATGACTCTCCTTGACACCTTGTTCTTCTATGTTATTCATTTTGTGGACAAGCTGGGAATATGGCACAGACTACCAGTTTTCATGGGACTTGCTTACTTGGGTATCAGAAGGCACTTGCACCAGCGTTACAATCTTTTGCATGTAGGGAGAATCAACGGTCAGAAATACGATACCGAAAGACTGTCCTATCGAACGGCTGACGGGACATGCAATCACCCTCATGATCATCTGGTCGGGAGCCAAGGAACCTGTTTCGGTCGCAACATGCCTCCCTCAACCTCACCCTACGGGTTGCTGGAGCCTCACCCTGCAATTGTAGCCTCCAAGCTTTTGGCCAGGAAGAAATTCGTAGACAATGGGAAGCAGTTCAACATGATAGCCTGTTCATGGATACAATTCATGATTCATGATTGGGTTGATCATTTGGAGGACACTGAGCAGGTGGAAATTAAAGCTCCTGAAGAAATCGCAACAGGGTGCCCTCTGAAATCATTCAAGTTTTATAAGACCAAGAAAGTTCCCACGGCTTCAACTTCTTCAGACTCTGATTGCTTGAATGCAAGGACTCCCTGGTGGGATGGGAGTGTAATTTATGGGAATAACATGGAGGGCATGCAAAGAGTAAGGACATTCAAAGATGGAAAGTTGAAGATTTCAGGAGATGGCCTTCTAGAGCATGATGAGAATGGTATTCCTATCTCCGGCGATGTTCGAAATTGCTGGGCTGGCTTCTCCCTCCTGCAAGCATTATTTGCCAAAGAACATAATGCTGCATGTGATATGCTAAAAGTACATTACCCAGATTTGGATGATGAGAAGCTGTACAGGCATGCAAGACTGGTCACTTCAGCTGTCATTGCTAAAATCCACACAATTGATTGGACTGTAGAGCTCTTAAAGACTGATACTCTTCTGGCAGGGATGAGGATCAACTGGTATGGATTTTTGGGGAAGAGATTTAAAGATTTACATGGACATATTTTAGGACCAATACTTAATGGATTGGTTGGCCTAAAGAAGCCCAGAGATCATGGAGTTCCCTATTCGCTGACTGAAGAGTTTGTAAGTGTGTACAGAATGCATGCCCTGCTACCAGATAAACTCATCCTTAGGGACATTGAGTCTACAGCTTCTGAAGACAAATGTCCTCCAATACTTGAAGAGGTGCTTATGAGAGATATGGCGGGTAAGGAGGGAGAACGAAGACTGTCGACAATTGGAATGGAGCAGATGCTCGTATCGATGGGTCACCAGGCATGTGGAGCTATCACATTATGGAATTATCCATCATGGATGAGGAACCTCATTGCACATGATGTTAATGGAGAAGATAGAGCCGATCCAGTTGATATGGCTGCATTAGAAATTTATAGAGACAGAGAGAGAGGAGTTGCCCGGTACAACGAGTTCAGAAGGAACCTGCTGATGATTCCTATTAACAAGTGGGAAGATTTGACGGATGACAAAGAAGTTGTTGAGGCTCTGAAGGAGGTGTATGGAGATGATGTTGAGAAGCTTGATTTGCAAGTGGGTCTACATGCAGAGAAGAAGATAAAGGGCTTTGCCATTAGTGAGACAGCTTTCTTCATCTTTCTACTTATTGCATCAAGGAGGCTAGAAGCTGATCGTTTCTTCACAACCAATTTCAACTCCAAAACATACACAGAGAAAGGCCTTGAATGGGTTAACAAGACAGAGACTTTGAAGGATGTGATTGATAGGCATTTCCCTGAAATGACAAGAAGATGGATGAAGTGTTCAAGTGCCTTTTCAGTGTGGGATTGGGAGCCAAACCCAGAAAGCTATATACCCTTATATTTGAGACCAGCTCCATAA

Protein Analysis

634

Amino Acids

72.64

Weight (kDa)

6.59

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73680 AT1G73680 AT1G73680 AT1G73680 AT3G01420
malus_domestica MD14G1234000.v1.1
prunus_persica Prupe.5G230200_v2.0.a1
pyrus_communis pycom14g19500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 570
AarI CACCTGC 1 cut(s) 496
Acc36I ACCTGC 2 cut(s) 496, 1494
AccB1I GGYRCC 1 cut(s) 543
AccI GTMKAC 4 cut(s) 417, 1190, 1277, 1600
AciI CCGC 1 cut(s) 1250
AclWI GGATC 2 cut(s) 1001, 1400
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 2 cut(s) 410, 1431
AcuI CTGAAG 6 cut(s) 546, 588, 897, 1140, 1221, 1571
AfaI GTAC 4 cut(s) 858, 890, 1139, 1466
AfiI CCNNNNNNNGG 4 cut(s) 453, 767, 871, 1747
AhdI GACNNNNNGTC 1 cut(s) 1186
AjnI CCWGG 3 cut(s) 401, 635, 1317
AloI GAACNNNNNNTCC 2 cut(s) 1807, 1839
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 4 cut(s) 1434, 1634, 1757, 1882
AlwI GGATC 2 cut(s) 1001, 1400
AlwNI CAGNNNCTG 2 cut(s) 611, 1199
AoxI GGCC 5 cut(s) 3, 399, 730, 1075, 1738
ApeKI GCWGC 2 cut(s) 836, 1421
ApoI RAATTY 2 cut(s) 410, 1431
ArsI GACNNNNNNTTYG 2 cut(s) 1750, 1782
Asp700I GAANNNNTTC 3 cut(s) 410, 431, 557
AspS9I GGNCC 1 cut(s) 1052
AsuC2I CCSGG 1 cut(s) 1462
AsuHPI GGTGA 4 cut(s) 272, 339, 362, 1307
AsuII TTCGAA 1 cut(s) 778
AvaII GGWCC 1 cut(s) 1052
AxyI CCTNAGG 1 cut(s) 1175
BaeGI GKGCMC 1 cut(s) 548
BaeI ACNNNNGTAYC 2 cut(s) 151, 184
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 543
BanII GRGCYC 1 cut(s) 960
BbsI GAAGAC 2 cut(s) 1209, 1276
Bbv12I GWGCWC 1 cut(s) 960
BbvI GCAGC 2 cut(s) 823, 1408
BccI CCATC 6 cut(s) 638, 698, 722, 1303, 1360, 1806
BceAI ACGGC 2 cut(s) 276, 606
BciT130I CCWGG 3 cut(s) 403, 637, 1319
BciVI GTATCC 1 cut(s) 450
BclI TGATCA 2 cut(s) 289, 484
BcnI CCSGG 1 cut(s) 1462
BcoDI GTCTC 4 cut(s) 1434, 1634, 1757, 1882
BfaI CTAG 2 cut(s) 737, 1682
BfmI CTRYAG 2 cut(s) 951, 1191
BfuAI ACCTGC 2 cut(s) 496, 1494
BfuI GTATCC 1 cut(s) 450
BisI GCNGC 2 cut(s) 837, 1422
BlsI GCNGC 2 cut(s) 838, 1423
Bme1390I CCNGG 4 cut(s) 403, 637, 1319, 1462
Bme18I GGWCC 1 cut(s) 1052
BmeRI GACNNNNNGTC 1 cut(s) 1186
BmgT120I GGNCC 1 cut(s) 1052
BmiI GGNNCC 7 cut(s) 305, 313, 366, 545, 1367, 1484, 1854
BmrFI CCNGG 4 cut(s) 403, 637, 1319, 1462
BmsI GCATC 2 cut(s) 1287, 1679
BoxI GACNNNNGTC 1 cut(s) 1209
BpiI GAAGAC 2 cut(s) 1209, 1276
BplI GAGNNNNNCTC 1 cut(s) 1879
BpmI CTGGAG 2 cut(s) 32, 383
Bpu14I TTCGAA 1 cut(s) 778
BpuMI CCSGG 1 cut(s) 1462
Bsa29I ATCGAT 1 cut(s) 1307
BsaBI GATNNNNATC 1 cut(s) 992
BsaI GGTCTC 1 cut(s) 1882
BsaJI CCNNGG 3 cut(s) 307, 588, 635
BsaXI ACNNNNNCTCC 2 cut(s) 1557, 1587
Bsc4I CCNNNNNNNGG 4 cut(s) 453, 767, 871, 1747
Bse1I ACTGG 4 cut(s) 140, 909, 1004, 1409
Bse21I CCTNAGG 1 cut(s) 1175
Bse3DI GCAATG 2 cut(s) 921, 1371
Bse8I GATNNNNATC 1 cut(s) 992
BseBI CCWGG 3 cut(s) 403, 637, 1319
BseCI ATCGAT 1 cut(s) 1307
BseDI CCNNGG 3 cut(s) 307, 588, 635
BseGI GGATG 9 cut(s) 37, 649, 880, 993, 1170, 1365, 1531, 1780, 1821
BseJI GATNNNNATC 1 cut(s) 992
BseLI CCNNNNNNNGG 4 cut(s) 453, 767, 871, 1747
BseMI GCAATG 2 cut(s) 921, 1371
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 4 cut(s) 140, 909, 1004, 1409
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 1 cut(s) 548
BseXI GCAGC 2 cut(s) 823, 1408
BseYI CCCAGC 2 cut(s) 118, 786
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 5 cut(s) 5, 401, 732, 1077, 1740
BshNI GGYRCC 1 cut(s) 543
BshVI ATCGAT 1 cut(s) 1307
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 960
BsiSI CCGG 2 cut(s) 768, 1462
BslFI GGGAC 3 cut(s) 165, 283, 1193
BslI CCNNNNNNNGG 4 cut(s) 453, 767, 871, 1747
BsmAI GTCTC 4 cut(s) 1434, 1634, 1757, 1882
BsmFI GGGAC 3 cut(s) 165, 283, 1193
BsmI GAATGC 2 cut(s) 628, 1149
BsnI GGCC 5 cut(s) 5, 401, 732, 1077, 1740
Bso31I GGTCTC 1 cut(s) 1882
Bsp119I TTCGAA 1 cut(s) 778
Bsp1286I GDGCHC 2 cut(s) 548, 960
Bsp1407I TGTACA 2 cut(s) 888, 1137
Bsp143I GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
BspACI CCGC 1 cut(s) 1250
BspANI GGCC 5 cut(s) 5, 401, 732, 1077, 1740
BspCNI CTCAG 1 cut(s) 493
BspDI ATCGAT 1 cut(s) 1307
BspHI TCATGA 3 cut(s) 286, 465, 472
BspLI GGNNCC 7 cut(s) 305, 313, 366, 545, 1367, 1484, 1854
BspMI ACCTGC 2 cut(s) 496, 1494
BspPI GGATC 2 cut(s) 1001, 1400
BspT104I TTCGAA 1 cut(s) 778
BspT107I GGYRCC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 1882
BsrDI GCAATG 2 cut(s) 921, 1371
BsrGI TGTACA 2 cut(s) 888, 1137
BsrI ACTGG 4 cut(s) 140, 909, 1004, 1409
BssECI CCNNGG 3 cut(s) 307, 588, 635
BssMI GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 403, 637, 1319
Bst4CI ACNGT 4 cut(s) 224, 249, 952, 1275
Bst6I CTCTTC 5 cut(s) 22, 981, 1013, 1116, 1221
BstAPI GCANNNNNTGC 2 cut(s) 818, 1153
BstAUI TGTACA 2 cut(s) 888, 1137
BstBI TTCGAA 1 cut(s) 778
BstC8I GCNNGC 5 cut(s) 680, 793, 810, 897, 1149
BstDEI CTNAG 2 cut(s) 501, 1175
BstDSI CCRYGG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 1313
BstF5I GGATG 9 cut(s) 37, 649, 880, 993, 1170, 1365, 1531, 1780, 1821
BstKTI GATC 6 cut(s) 292, 487, 996, 1096, 1408, 1693
BstMAI GTCTC 4 cut(s) 1434, 1634, 1757, 1882
BstMBI GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 8 cut(s) 383, 792, 818, 892, 923, 1153, 1328, 1588
BstNI CCWGG 3 cut(s) 403, 637, 1319
BstNSI RCATGY 9 cut(s) 209, 276, 334, 682, 843, 899, 1151, 1326, 1607
BstPAI GACNNNNGTC 1 cut(s) 1209
BstPI GGTNACC 1 cut(s) 1313
BstSCI CCNGG 4 cut(s) 401, 635, 1317, 1460
BstSFI CTRYAG 2 cut(s) 951, 1191
BstSLI GKGCMC 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 823, 1408
BstV2I GAAGAC 2 cut(s) 1209, 1276
BstXI CCANNNNNNTGG 2 cut(s) 397, 1325
Bsu15I ATCGAT 1 cut(s) 1307
Bsu36I CCTNAGG 1 cut(s) 1175
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 5 cut(s) 5, 401, 732, 1077, 1740
BsuTUI ATCGAT 1 cut(s) 1307
BtgI CCRYGG 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 786
BtsCI GGATG 9 cut(s) 37, 649, 880, 993, 1170, 1365, 1531, 1780, 1821
BtsIMutI CAGTG 2 cut(s) 498, 1845
BveI ACCTGC 2 cut(s) 496, 1494
Cac8I GCNNGC 5 cut(s) 680, 793, 810, 897, 1149
CaiI CAGNNNCTG 2 cut(s) 611, 1199
CciI TCATGA 3 cut(s) 286, 465, 472
Cfr13I GGNCC 1 cut(s) 1052
ClaI ATCGAT 1 cut(s) 1307
Csp6I GTAC 4 cut(s) 857, 889, 1138, 1465
CviQI GTAC 4 cut(s) 857, 889, 1138, 1465
DdeI CTNAG 2 cut(s) 501, 1175
DpnI GATC 6 cut(s) 291, 486, 995, 1095, 1407, 1692
DpnII GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
DraI TTTAAA 1 cut(s) 1027
DriI GACNNNNNGTC 1 cut(s) 1186
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 5 cut(s) 22, 981, 1013, 1116, 1221
Eam1105I GACNNNNNGTC 1 cut(s) 1186
EarI CTCTTC 5 cut(s) 22, 981, 1013, 1116, 1221
Ecl136II GAGCTC 1 cut(s) 958
Eco130I CCWWGG 1 cut(s) 307
Eco147I AGGCCT 1 cut(s) 1740
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 1882
Eco47I GGWCC 1 cut(s) 1052
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 6 cut(s) 546, 588, 897, 1140, 1221, 1571
Eco81I CCTNAGG 1 cut(s) 1175
Eco91I GGTNACC 1 cut(s) 1313
EcoICRI GAGCTC 1 cut(s) 958
EcoO65I GGTNACC 1 cut(s) 1313
EcoRII CCWGG 3 cut(s) 401, 635, 1317
EcoT14I CCWWGG 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 1149
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 307
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 3 cut(s) 165, 283, 1193
FauI CCCGC 1 cut(s) 1243
FbaI TGATCA 2 cut(s) 289, 484
FblI GTMKAC 4 cut(s) 417, 1190, 1277, 1600
Fnu4HI GCNGC 2 cut(s) 837, 1422
FokI GGATG 9 cut(s) 24, 656, 887, 1000, 1157, 1372, 1538, 1787, 1828
FriOI GRGCYC 1 cut(s) 960
Fsp4HI GCNGC 2 cut(s) 837, 1422
FspBI CTAG 2 cut(s) 737, 1682
GluI GCNGC 2 cut(s) 837, 1422
GsaI CCCAGC 2 cut(s) 122, 790
GsuI CTGGAG 2 cut(s) 32, 383
HaeIII GGCC 5 cut(s) 5, 401, 732, 1077, 1740
HapII CCGG 2 cut(s) 768, 1462
HincII GTYRAC 2 cut(s) 1278, 1753
HindII GTYRAC 2 cut(s) 1278, 1753
HindIII AAGCTT 2 cut(s) 392, 1580
HinfI GANTC 7 cut(s) 70, 216, 469, 608, 631, 1187, 1495
HpaI GTTAAC 1 cut(s) 1753
HpaII CCGG 2 cut(s) 768, 1462
HphI GGTGA 4 cut(s) 272, 339, 362, 1307
Hpy166II GTNNAC 7 cut(s) 112, 418, 1138, 1191, 1278, 1601, 1753
Hpy188I TCNGA 8 cut(s) 173, 228, 552, 607, 613, 1201, 1478, 1551
Hpy188III TCNNGA 7 cut(s) 287, 301, 466, 473, 524, 723, 737
Hpy8I GTNNAC 7 cut(s) 112, 418, 1138, 1191, 1278, 1601, 1753
HpyAV CCTTC 7 cut(s) 16, 40, 168, 743, 1473, 1546, 1765
HpyCH4III ACNGT 4 cut(s) 224, 249, 952, 1275
HpyF10VI GCNNNNNNNGC 8 cut(s) 383, 792, 818, 892, 923, 1153, 1328, 1588
HpyF3I CTNAG 2 cut(s) 501, 1175
Ksp22I TGATCA 2 cut(s) 289, 484
KspAI GTTAAC 1 cut(s) 1753
Kzo9I GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
LmnI GCTCC 7 cut(s) 303, 364, 526, 1291, 1328, 1852, 1900
Lsp1109I GCAGC 2 cut(s) 823, 1408
LweI GCATC 2 cut(s) 1287, 1679
MaeI CTAG 2 cut(s) 737, 1682
MaeIII GTNAC 3 cut(s) 191, 907, 1313
MalI GATC 6 cut(s) 291, 486, 995, 1095, 1407, 1692
MboI GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
MfeI CAATTG 3 cut(s) 378, 939, 1281
MhlI GDGCHC 2 cut(s) 548, 960
MlsI TGGCCA 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 4 cut(s) 64, 602, 625, 1196
Mox20I TGGCCA 1 cut(s) 401
Mph1103I ATGCAT 1 cut(s) 1149
MroXI GAANNNNTTC 3 cut(s) 410, 431, 557
MscI TGGCCA 1 cut(s) 401
MseI TTAA 7 cut(s) 516, 962, 1026, 1062, 1386, 1503, 1752
MslI CAYNNNNRTG 1 cut(s) 285
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 2 cut(s) 768, 1462
MspR9I CCNGG 4 cut(s) 403, 637, 1319, 1462
MunI CAATTG 3 cut(s) 378, 939, 1281
Mva1269I GAATGC 2 cut(s) 628, 1149
MvaI CCWGG 3 cut(s) 403, 637, 1319
MwoI GCNNNNNNNGC 8 cut(s) 383, 792, 818, 892, 923, 1153, 1328, 1588
NciI CCSGG 1 cut(s) 1462
NdeII GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
NlaIV GGNNCC 7 cut(s) 305, 313, 366, 545, 1367, 1484, 1854
NmuCI GTSAC 2 cut(s) 907, 1313
NsiI ATGCAT 1 cut(s) 1149
NspI RCATGY 9 cut(s) 209, 276, 334, 682, 843, 899, 1151, 1326, 1607
NspV TTCGAA 1 cut(s) 778
PaeI GCATGC 3 cut(s) 682, 899, 1151
PagI TCATGA 3 cut(s) 286, 465, 472
PaqCI CACCTGC 1 cut(s) 496
PceI AGGCCT 1 cut(s) 1740
PctI GAATGC 2 cut(s) 628, 1149
PdmI GAANNNNTTC 3 cut(s) 410, 431, 557
PfeI GAWTC 3 cut(s) 216, 469, 1495
PkrI GCNGC 2 cut(s) 838, 1423
PleI GAGTC 4 cut(s) 64, 602, 625, 1195
PpsI GAGTC 4 cut(s) 64, 602, 625, 1195
PshAI GACNNNNGTC 1 cut(s) 1209
PsiI TTATAA 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 960
Psp6I CCWGG 3 cut(s) 401, 635, 1317
PspEI GGTNACC 1 cut(s) 1313
PspFI CCCAGC 2 cut(s) 118, 786
PspGI CCWGG 3 cut(s) 401, 635, 1317
PspN4I GGNNCC 7 cut(s) 305, 313, 366, 545, 1367, 1484, 1854
PspPI GGNCC 1 cut(s) 1052
PsrI GAACNNNNNNTAC 2 cut(s) 1457, 1489
PstNI CAGNNNCTG 2 cut(s) 611, 1199
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 4 cut(s) 858, 890, 1139, 1466
RsaNI GTAC 4 cut(s) 857, 889, 1138, 1465
RseI CAYNNNNRTG 1 cut(s) 285
SacI GAGCTC 1 cut(s) 960
SalI GTCGAC 1 cut(s) 1276
SaqAI TTAA 7 cut(s) 516, 962, 1026, 1062, 1386, 1503, 1752
SatI GCNGC 2 cut(s) 837, 1422
Sau3AI GATC 6 cut(s) 289, 484, 993, 1093, 1405, 1690
Sau96I GGNCC 1 cut(s) 1052
SchI GAGTC 4 cut(s) 64, 602, 625, 1196
ScrFI CCNGG 4 cut(s) 403, 637, 1319, 1462
SduI GDGCHC 2 cut(s) 548, 960
SfaNI GCATC 2 cut(s) 1287, 1679
SfcI CTRYAG 2 cut(s) 951, 1191
SfuI TTCGAA 1 cut(s) 778
SinI GGWCC 1 cut(s) 1052
SmiMI CAYNNNNRTG 1 cut(s) 285
SphI GCATGC 3 cut(s) 682, 899, 1151
SseBI AGGCCT 1 cut(s) 1740
SsiI CCGC 1 cut(s) 1250
SspMI CTAG 2 cut(s) 737, 1682
SstI GAGCTC 1 cut(s) 960
StuI AGGCCT 1 cut(s) 1740
StyD4I CCNGG 4 cut(s) 401, 635, 1317, 1460
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 4 cut(s) 224, 249, 952, 1275
TaqI TCGA 4 cut(s) 256, 778, 1277, 1307
TaqII GACCGA 1 cut(s) 311
TatI WGTACW 3 cut(s) 856, 888, 1137
TfiI GAWTC 3 cut(s) 216, 469, 1495
Tru1I TTAA 7 cut(s) 516, 962, 1026, 1062, 1386, 1503, 1752
Tru9I TTAA 7 cut(s) 516, 962, 1026, 1062, 1386, 1503, 1752
TscAI CASTG 2 cut(s) 505, 1845
TseFI GTSAC 2 cut(s) 907, 1313
TseI GCWGC 2 cut(s) 836, 1421
Tsp45I GTSAC 2 cut(s) 907, 1313
TspDTI ATGAA 8 cut(s) 26, 92, 136, 441, 454, 461, 1642, 1832
TspGWI ACGGA 1 cut(s) 1538
TspRI CASTG 2 cut(s) 505, 1845
VpaK11BI GGWCC 1 cut(s) 1052
XapI RAATTY 2 cut(s) 410, 1431
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 9 cut(s) 209, 276, 334, 682, 843, 899, 1151, 1326, 1607
XmiI GTMKAC 4 cut(s) 417, 1190, 1277, 1600
XmnI GAANNNNTTC 3 cut(s) 410, 431, 557
XspI CTAG 2 cut(s) 737, 1682
Zsp2I ATGCAT 1 cut(s) 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.