pycom14g19500

alpha-dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019156
Physical Location & Seq
Forward (+)
20798248 .. 20800071
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g19500.5

Sequence Viewer

Length: 1296 bp
ATGGAGGGCATGAAGAGAGTAAGGACATTCAAAAATGGAAAGTTGAAGATTTCCAGAGATGGGCTCCTTCAGCATGACGAAAAAGGTATCCCTATCTCTGGTGATGTTCGAAATTGCTGGGCTGGATTCTCCCTTCTCCAAGCATTATTTGCCAAAGAACATAATTCTGTATGTGACATGCTCAAAGTATGTCACCCAGATTTGGATGATGAGAATCTCTACAGGCATGCAAGATTGGTGACTTCAGCTGTCATTGCTAAAATCCACACAATTGATTGGACTGTCGAACTCTTAAAGACCGATACTCTTGTGGCCGGAATGAGAATCAACTGGTATGGCTTTCTGGGGAAGAGGTTCAAAGATTTTTGTGGAAATATATTAGGACCAATACTTACTGGATTGGTTGGTCATAAGAAGCCTAGATATCATGGAGTTCCGTATTCACTGACCGAAGAGTTTGTAAGCGTGTACAGAATGCATTCGCTACTACCAGATAAACTCATCGTTAGGGACATTCAGTCTACAGATTCGGAAGACAAATGCCCTCCCATACTAGAAGAGATGCTTATGAGAGATATGGCGGGCAAGGAAGGAGAACGAAGACTGTCAAAAATCGGGATGGAGCAAATGCTGGTATCGATGGGTCACCAGGCATGTGGAGCTGTCACATTGTGGAACTATCCCTCATGGTTGAGGGACCTCGTTGCACATGACAGTAACGGAGAAGATCGAGAAGATCCAGTTGATATGGCTGCATTAGAAATTTACAGAGACAGAGAGAGAGGAGTTGCGCGGTACAACGAGTTCAGAAGGAACCTGCTGATGATTCCTATTAGCAGGTGGGAAGATTTGACAGATGATGAAGAAGTTACAGAAGCTCTTAAGGAGGTGTATGGAGATGACGTCGAGAAGCTTGATTTGCTAGTCGGACTACATGCGGAGAAGAAGATAAAGGGCTTCGCCATTAGCGAGACGGCTTTCTTCATCTTTCTGCTTATTGCATCAAGGTGCGTATGCGGTTGTAAAATGCTTACATTTCTACTTATATGTGCAATCGGCAATGTAATTAGGAGGCTAGAAGCTGATCGTTTCTTCACGACGAATTTCAACTCGAAGACATACACGGAGAAAGGCCTCGAATGGGTTAACAAGACCGAGACTTTGAAGGATGTGATCGATAGGCATTTTCCAGACATGACAGGGAGATGGATGAAGTGTTCGAGTGCCTTTTCGGTGTGGGATTGGGAGCCAAATCCAGAAAGCTACTTACCCTTATATCTGAGACCAGCTCCATGA

Protein Analysis

432

Amino Acids

49.55

Weight (kDa)

6.1

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73680 AT1G73680 AT1G73680 AT1G73680 AT3G01420
malus_domestica MD14G1234000.v1.1
prunus_persica Prupe.5G230200_v2.0.a1
pyrus_communis pycom14g19500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 828
AatII GACGTC 1 cut(s) 906
Acc36I ACCTGC 2 cut(s) 825, 828
AccI GTMKAC 1 cut(s) 521
AccII CGCG 1 cut(s) 793
AciI CCGC 4 cut(s) 581, 793, 938, 1017
AclWI GGATC 1 cut(s) 731
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 2 cut(s) 762, 1102
AcuI CTGAAG 2 cut(s) 53, 228
AcyI GRCGYC 1 cut(s) 903
AdeI CACNNNGTG 1 cut(s) 672
AfaI GTAC 2 cut(s) 470, 797
AfiI CCNNNNNNNGG 4 cut(s) 60, 98, 202, 1141
AflII CTTAAG 1 cut(s) 881
AgsI TTSAA 5 cut(s) 31, 46, 358, 1108, 1165
AhdI GACNNNNNGTC 1 cut(s) 517
AjnI CCWGG 1 cut(s) 648
AloI GAACNNNNNNTCC 2 cut(s) 1201, 1233
AluBI AGCT 7 cut(s) 248, 662, 878, 913, 1082, 1263, 1289
AluI AGCT 7 cut(s) 248, 662, 878, 913, 1082, 1263, 1289
Alw26I GTCTC 4 cut(s) 765, 965, 1151, 1276
AlwI GGATC 1 cut(s) 731
AoxI GGCC 2 cut(s) 312, 1132
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 762, 1102
ArsI GACNNNNNNTTYG 2 cut(s) 1144, 1176
Asp700I GAANNNNTTC 2 cut(s) 353, 478
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 2 cut(s) 383, 697
AsuHPI GGTGA 4 cut(s) 113, 185, 250, 638
AsuII TTCGAA 1 cut(s) 109
AvaII GGWCC 2 cut(s) 383, 697
BaeI ACNNNNGTAYC 2 cut(s) 294, 327
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 3 cut(s) 540, 607, 1121
BbvI GCAGC 1 cut(s) 739
BccI CCATC 4 cut(s) 53, 613, 634, 1200
BceAI ACGGC 1 cut(s) 990
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 765, 965, 1151, 1276
BfaI CTAG 4 cut(s) 420, 554, 923, 1076
BfmI CTRYAG 2 cut(s) 220, 522
BfrI CTTAAG 1 cut(s) 881
BfuAI ACCTGC 2 cut(s) 825, 828
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 1 cut(s) 753
BlsI GCNGC 1 cut(s) 754
Bme1390I CCNGG 1 cut(s) 650
Bme18I GGWCC 2 cut(s) 383, 697
BmeRI GACNNNNNGTC 1 cut(s) 517
BmgT120I GGNCC 2 cut(s) 383, 697
BmiI GGNNCC 4 cut(s) 65, 698, 815, 1248
BmrFI CCNGG 1 cut(s) 650
BmsI GCATC 2 cut(s) 552, 1010
BpiI GAAGAC 3 cut(s) 540, 607, 1121
BplI GAGNNNNNCTC 3 cut(s) 48, 80, 1273
Bpu14I TTCGAA 1 cut(s) 109
Bsa29I ATCGAT 2 cut(s) 638, 1176
BsaBI GATNNNNATC 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 903
BsaI GGTCTC 1 cut(s) 1276
BsaXI ACNNNNNCTCC 2 cut(s) 888, 918
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 98, 202, 1141
Bse1I ACTGG 3 cut(s) 335, 400, 740
Bse3DI GCAATG 2 cut(s) 252, 1066
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 1 cut(s) 650
BseCI ATCGAT 2 cut(s) 638, 1176
BseGI GGATG 4 cut(s) 211, 624, 1174, 1215
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 4 cut(s) 60, 98, 202, 1141
BseMI GCAATG 2 cut(s) 252, 1066
BseMII CTCAG 1 cut(s) 1271
BseNI ACTGG 3 cut(s) 335, 400, 740
BseRI GAGGAG 1 cut(s) 798
BseXI GCAGC 1 cut(s) 739
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 793
BshFI GGCC 2 cut(s) 314, 1134
BshVI ATCGAT 2 cut(s) 638, 1176
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 2 cut(s) 524, 710
BslI CCNNNNNNNGG 4 cut(s) 60, 98, 202, 1141
BsmAI GTCTC 4 cut(s) 765, 965, 1151, 1276
BsmBI CGTCTC 1 cut(s) 965
BsmFI GGGAC 2 cut(s) 524, 710
BsmI GAATGC 2 cut(s) 478, 480
BsnI GGCC 2 cut(s) 314, 1134
Bso31I GGTCTC 1 cut(s) 1276
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 4 cut(s) 727, 736, 1084, 1173
BspACI CCGC 4 cut(s) 581, 793, 938, 1017
BspANI GGCC 2 cut(s) 314, 1134
BspCNI CTCAG 1 cut(s) 1272
BspDI ATCGAT 2 cut(s) 638, 1176
BspFNI CGCG 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 65, 698, 815, 1248
BspMI ACCTGC 2 cut(s) 825, 828
BspPI GGATC 1 cut(s) 731
BspT104I TTCGAA 1 cut(s) 109
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 1276
BsrDI GCAATG 2 cut(s) 252, 1066
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 3 cut(s) 335, 400, 740
BssMI GATC 4 cut(s) 727, 736, 1084, 1173
BssNI GRCGYC 1 cut(s) 903
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 3 cut(s) 283, 606, 716
Bst6I CTCTTC 4 cut(s) 8, 344, 447, 552
BstACI GRCGYC 1 cut(s) 903
BstAFI CTTAAG 1 cut(s) 881
BstAPI GCANNNNNTGC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 228, 583
BstDEI CTNAG 1 cut(s) 1280
BstEII GGTNACC 1 cut(s) 644
BstF5I GGATG 4 cut(s) 211, 624, 1174, 1215
BstFNI CGCG 1 cut(s) 793
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 4 cut(s) 730, 739, 1087, 1176
BstMAI GTCTC 4 cut(s) 765, 965, 1151, 1276
BstMBI GATC 4 cut(s) 727, 736, 1084, 1173
BstMWI GCNNNNNNNGC 5 cut(s) 70, 149, 254, 659, 919
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 4 cut(s) 181, 230, 657, 938
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 648
BstSFI CTRYAG 2 cut(s) 220, 522
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 1 cut(s) 739
BstV2I GAAGAC 3 cut(s) 540, 607, 1121
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 1 cut(s) 736
Bsu15I ATCGAT 2 cut(s) 638, 1176
BsuI GTATCC 1 cut(s) 98
BsuRI GGCC 2 cut(s) 314, 1134
BsuTUI ATCGAT 2 cut(s) 638, 1176
BtsCI GGATG 4 cut(s) 211, 624, 1174, 1215
BtsIMutI CAGTG 1 cut(s) 443
BveI ACCTGC 2 cut(s) 825, 828
Cac8I GCNNGC 2 cut(s) 228, 583
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 383, 697
ClaI ATCGAT 2 cut(s) 638, 1176
Csp6I GTAC 2 cut(s) 469, 796
CviQI GTAC 2 cut(s) 469, 796
DdeI CTNAG 1 cut(s) 1280
DpnI GATC 4 cut(s) 729, 738, 1086, 1175
DpnII GATC 4 cut(s) 727, 736, 1084, 1173
DraIII CACNNNGTG 1 cut(s) 672
DriI GACNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 4 cut(s) 8, 344, 447, 552
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 4 cut(s) 8, 344, 447, 552
Eco147I AGGCCT 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 1276
Eco32I GATATC 1 cut(s) 425
Eco47I GGWCC 2 cut(s) 383, 697
Eco57I CTGAAG 2 cut(s) 53, 228
Eco91I GGTNACC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 697
EcoO65I GGTNACC 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 1 cut(s) 425
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 965
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 2 cut(s) 524, 710
FauI CCCGC 1 cut(s) 574
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 4 cut(s) 218, 631, 1181, 1222
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 4 cut(s) 420, 554, 923, 1076
GlaI GCGC 1 cut(s) 792
GluI GCNGC 1 cut(s) 753
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 314, 1134
HapII CCGG 1 cut(s) 315
HhaI GCGC 1 cut(s) 793
Hin1I GRCGYC 1 cut(s) 903
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 5 cut(s) 126, 214, 324, 527, 826
HpaI GTTAAC 1 cut(s) 1147
HpaII CCGG 1 cut(s) 315
HphI GGTGA 4 cut(s) 113, 185, 250, 638
Hpy166II GTNNAC 3 cut(s) 469, 522, 1147
Hpy188I TCNGA 4 cut(s) 532, 809, 929, 1281
Hpy188III TCNNGA 7 cut(s) 54, 616, 731, 907, 1096, 1190, 1256
Hpy8I GTNNAC 3 cut(s) 469, 522, 1147
Hpy99I CGWCG 2 cut(s) 908, 1102
HpyAV CCTTC 5 cut(s) 77, 143, 584, 804, 1159
HpyCH4III ACNGT 3 cut(s) 283, 606, 716
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 6 cut(s) 230, 478, 707, 755, 1001, 1052
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 149, 254, 659, 919
HpyF3I CTNAG 1 cut(s) 1280
HpySE526I ACGT 1 cut(s) 903
Hsp92I GRCGYC 1 cut(s) 903
HspAI GCGC 1 cut(s) 791
KspAI GTTAAC 1 cut(s) 1147
Kzo9I GATC 4 cut(s) 727, 736, 1084, 1173
LmnI GCTCC 5 cut(s) 69, 622, 659, 1246, 1294
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 2 cut(s) 552, 1010
MaeI CTAG 4 cut(s) 420, 554, 923, 1076
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 7 cut(s) 173, 191, 238, 644, 664, 716, 868
MalI GATC 4 cut(s) 729, 738, 1086, 1175
MboI GATC 4 cut(s) 727, 736, 1084, 1173
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 6 cut(s) 112, 163, 270, 762, 1065, 1102
MmeI TCCRAC 1 cut(s) 907
MnlI CCTC 9 cut(s) 345, 555, 687, 694, 710, 776, 880, 1065, 1145
Mph1103I ATGCAT 1 cut(s) 480
MroXI GAANNNNTTC 2 cut(s) 353, 478
MseI TTAA 3 cut(s) 293, 882, 1146
MslI CAYNNNNRTG 1 cut(s) 1006
MspA1I CMGCKG 1 cut(s) 248
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 650
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 2 cut(s) 478, 480
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 5 cut(s) 70, 149, 254, 659, 919
NdeII GATC 4 cut(s) 727, 736, 1084, 1173
NlaIV GGNNCC 4 cut(s) 65, 698, 815, 1248
NmuCI GTSAC 5 cut(s) 173, 191, 238, 644, 664
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 4 cut(s) 181, 230, 657, 938
NspV TTCGAA 1 cut(s) 109
PaeI GCATGC 1 cut(s) 230
PaqCI CACCTGC 1 cut(s) 828
PceI AGGCCT 1 cut(s) 1134
PcsI WCGNNNNNNNCGW 1 cut(s) 966
PctI GAATGC 2 cut(s) 478, 480
PdmI GAANNNNTTC 2 cut(s) 353, 478
PfeI GAWTC 5 cut(s) 126, 214, 324, 527, 826
PkrI GCNGC 1 cut(s) 754
PpuMI RGGWCCY 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 648
PspEI GGTNACC 1 cut(s) 644
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 65, 698, 815, 1248
PspPI GGNCC 2 cut(s) 383, 697
PspPPI RGGWCCY 1 cut(s) 697
PsrI GAACNNNNNNTAC 2 cut(s) 788, 820
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 2 cut(s) 470, 797
RsaNI GTAC 2 cut(s) 469, 796
RseI CAYNNNNRTG 1 cut(s) 1006
SaqAI TTAA 3 cut(s) 293, 882, 1146
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 4 cut(s) 727, 736, 1084, 1173
Sau96I GGNCC 2 cut(s) 383, 697
ScrFI CCNGG 1 cut(s) 650
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 2 cut(s) 552, 1010
SfcI CTRYAG 2 cut(s) 220, 522
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 2 cut(s) 383, 697
SmiMI CAYNNNNRTG 1 cut(s) 1006
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SphI GCATGC 1 cut(s) 230
Sse9I AATT 6 cut(s) 112, 163, 270, 762, 1065, 1102
SseBI AGGCCT 1 cut(s) 1134
SsiI CCGC 4 cut(s) 581, 793, 938, 1017
SspMI CTAG 4 cut(s) 420, 554, 923, 1076
StuI AGGCCT 1 cut(s) 1134
StyD4I CCNGG 1 cut(s) 648
TaaI ACNGT 3 cut(s) 283, 606, 716
TaiI ACGT 1 cut(s) 906
TaqI TCGA 9 cut(s) 109, 285, 638, 730, 906, 1112, 1137, 1176, 1220
TaqII GACCGA 3 cut(s) 314, 464, 1169
TasI AATT 6 cut(s) 112, 163, 270, 762, 1065, 1102
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 5 cut(s) 126, 214, 324, 527, 826
Tru1I TTAA 3 cut(s) 293, 882, 1146
Tru9I TTAA 3 cut(s) 293, 882, 1146
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 5 cut(s) 173, 191, 238, 644, 664
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 5 cut(s) 173, 191, 238, 644, 664
TspDTI ATGAA 4 cut(s) 26, 876, 973, 1226
TspGWI ACGGA 3 cut(s) 426, 735, 1139
TspRI CASTG 1 cut(s) 450
Vha464I CTTAAG 1 cut(s) 881
VpaK11BI GGWCC 2 cut(s) 383, 697
XapI RAATTY 2 cut(s) 762, 1102
XceI RCATGY 4 cut(s) 181, 230, 657, 938
XmiI GTMKAC 1 cut(s) 521
XmnI GAANNNNTTC 2 cut(s) 353, 478
XspI CTAG 4 cut(s) 420, 554, 923, 1076
ZraI GACGTC 1 cut(s) 904
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.