Prupe.6G190400_v2.0.a1

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0028949
Physical Location & Seq
Forward (+)
19772020 .. 19775166
3147 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G190400.1

Sequence Viewer

Length: 192 bp
ATGTGTGGGAGGGCTCGCTGTACTCTCAGAGCCGACGACATCCCTAGGGCTTGCCACAGCAGCCATGGCCCCGTGTGTACTGTTAACATGGACCGGTTTCGCCCGCTGTTCAATGCGTCGCCGGGGTCGAATTTACCTGTGTTCGTAGAGAAGACGGAGCAGACGGTGACGGCGTTGTTGTTCACTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

64

Amino Acids

6.94

Weight (kDa)

8.52

Isoelectric Point (pI)

56.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028949)

Species Orthologous Gene IDs
prunus_persica Prupe.2G270900_v2.0.a1 Prupe.6G190400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 104
AcsI RAATTY 1 cut(s) 130
AfaI GTAC 2 cut(s) 22, 79
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 1 cut(s) 112
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 60
ApoI RAATTY 1 cut(s) 130
AsiGI ACCGGT 1 cut(s) 93
AspA2I CCTAGG 1 cut(s) 44
AspS9I GGNCC 2 cut(s) 68, 91
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 91
AvrII CCTAGG 1 cut(s) 44
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 72
BceAI ACGGC 1 cut(s) 186
BcnI CCSGG 1 cut(s) 123
BfaI CTAG 1 cut(s) 45
BisI GCNGC 1 cut(s) 61
BlnI CCTAGG 1 cut(s) 44
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 68, 91
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 44, 64, 122
BsaWI WCCGGW 1 cut(s) 93
Bse118I RCCGGY 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 44, 64, 122
BseGI GGATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 72
BshFI GGCC 1 cut(s) 69
BshTI ACCGGT 1 cut(s) 93
BsiSI CCGG 2 cut(s) 94, 122
BsnI GGCC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 16
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 44, 64, 122
BssT1I CCWWGG 2 cut(s) 44, 64
Bst4CI ACNGT 3 cut(s) 82, 166, 187
BstC8I GCNNGC 3 cut(s) 16, 52, 104
BstDEI CTNAG 1 cut(s) 26
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 39
BstMWI GCNNNNNNNGC 2 cut(s) 60, 66
BstSCI CCNGG 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 158
BsuRI GGCC 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 16, 52, 104
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 68, 91
CseI GACGC 1 cut(s) 105
Csp6I GTAC 2 cut(s) 21, 78
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 2 cut(s) 65, 88
CviJI RGCY 5 cut(s) 14, 32, 50, 63, 69
CviKI_1 RGCY 5 cut(s) 14, 32, 50, 63, 69
CviQI GTAC 2 cut(s) 21, 78
DdeI CTNAG 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 44, 64
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 91
EcoT14I CCWWGG 2 cut(s) 44, 64
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 44, 64
FaeI CATG 2 cut(s) 68, 91
FaiI YATR 3 cut(s) 66, 89, 190
FatI CATG 2 cut(s) 64, 87
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 1 cut(s) 45
GluI GCNGC 1 cut(s) 61
HaeIII GGCC 1 cut(s) 69
HapII CCGG 2 cut(s) 94, 122
HgaI GACGC 1 cut(s) 105
Hin1II CATG 2 cut(s) 68, 91
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HpaI GTTAAC 1 cut(s) 85
HpaII CCGG 2 cut(s) 94, 122
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 3 cut(s) 78, 85, 183
Hpy188I TCNGA 1 cut(s) 29
Hpy8I GTNNAC 3 cut(s) 78, 85, 183
Hpy99I CGWCG 2 cut(s) 38, 121
HpyCH4III ACNGT 3 cut(s) 82, 166, 187
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 66
HpyF3I CTNAG 1 cut(s) 26
Hsp92II CATG 2 cut(s) 68, 91
KspAI GTTAAC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 3 cut(s) 107, 135, 150
Lsp1109I GCAGC 1 cut(s) 72
MaeI CTAG 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 166
MboII GAAGA 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 1 cut(s) 130
MnlI CCTC 1 cut(s) 3
MseI TTAA 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 2 cut(s) 94, 122
MspR9I CCNGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 60, 66
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 64
NlaIII CATG 2 cut(s) 68, 91
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 170
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 2 cut(s) 68, 91
RsaI GTAC 2 cut(s) 22, 79
RsaNI GTAC 2 cut(s) 21, 78
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 61
Sau96I GGNCC 2 cut(s) 68, 91
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 16
SetI ASST 1 cut(s) 139
SinI GGWCC 1 cut(s) 91
Sse9I AATT 1 cut(s) 130
SsiI CCGC 1 cut(s) 104
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 2 cut(s) 44, 64
TaaI ACNGT 3 cut(s) 82, 166, 187
TaqI TCGA 1 cut(s) 128
TasI AATT 1 cut(s) 130
TatI WGTACW 2 cut(s) 20, 77
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XmaJI CCTAGG 1 cut(s) 44
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.