Prupe.7G002400_v2.0.a1

HNH endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012307
Physical Location & Seq
Reverse (-)
305019 .. 312152
7134 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G002400.3

Sequence Viewer

Length: 840 bp
ATGGCTCAGTTTGCAGCACAAGGGCGAGTTAAGTTGCTCTTTAATGGCGAGGGAGTGGCATTGGAACAAAAAGGAGACCCATTTAACAACAAAATGTTCACATCAATCCAACCCCACACCTACAACCGCAGGCTTGGCAGGCCACACCTGGATTCTACTCGGCTTTACTCTTCTTCTTCTATGAACAAGGCAAAGCGTTTGAATGCTGAGGCCCGTTTGTTTGTTGGTGGCGATGAGGCTGGTCCTGGTCATGACGATGCTGCTGAGTTTGAGAGGGATGATGAGTTGGCTTGTTTCAGAGGCCTCGTCTTGGATATCTCCTACAGGCCAGTTAATGTTGTCTGCTGGAAGCGTGCTATTTGCTTGGAATACATGGAGAAGGCGGATGTACTTGAATACTATGATCAGACTGTAAATTCCCCAAATGGATCCTTCTACATACCTGCCGTCCTAAGGGTTCCACATTTACTGCAGATTGTAAAGAGAAGAAGAATTAAGAACAATCTTAGTCGTAAAAATATACTATGTCGGGACAGTTTCACTTGTCAGTACTGTTCTTCGCGTGAAAACTTGACTATTGACCATGTTTTGCCAATTGTACGTGGTGGAGAATGGAAGTGGGAAAATCTGGTCACTGCATGTGCAAGATGCAACTCGAAGAAAGGTCAGAAGACATTAGAGGAAGCAAATATGAAGCTTACTAAAGTCCCCAAGGCTCCAAAAGACTATGACATACTTGCCATACCACTGACAAGTGCCGCAATTAAAATGCTGAAAATGAGAAAGGGAACTCCTGAAGAGTGGCTCCAATACTTATCAAAGCCTTCTTCAGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH_5

Protein Analysis

280

Amino Acids

31.76

Weight (kDa)

9.25

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23840
fragaria_vesca FvH4_3g25250 FvH4_3g25250
malus_domestica MD12G1002500.v1.1
prunus_persica Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1 Prupe.7G002400_v2.0.a1
pyrus_communis pycom12g00220
rosa_chinensis RchiOBHm_Chr5g0046891
rosa_laevigata RLG00000034453
rosa_multiflora Rmu_sc0005233.1_g000003
rosa_roxburghii Rroxscaffold_1G00034500
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311900 Rh5BG320900 Rh5CG347100 Rh5DG331600
rosa_wichuraiana Rw5G029150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccII CGCG 1 cut(s) 562
AciI CCGC 3 cut(s) 127, 383, 759
AclWI GGATC 2 cut(s) 423, 436
AcsI RAATTY 1 cut(s) 415
AcuI CTGAAG 2 cut(s) 813, 816
AfaI GTAC 3 cut(s) 390, 551, 600
AfiI CCNNNNNNNGG 2 cut(s) 310, 453
AgsI TTSAA 2 cut(s) 202, 395
AjnI CCWGG 2 cut(s) 147, 244
AluBI AGCT 1 cut(s) 697
AluI AGCT 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 2 cut(s) 423, 436
AoxI GGCC 4 cut(s) 140, 210, 301, 326
ApeKI GCWGC 2 cut(s) 14, 260
ApoI RAATTY 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 211, 242
AvaII GGWCC 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 452
BamHI GGATCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 677
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 26, 247
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 2 cut(s) 149, 246
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 322, 470
BfuAI ACCTGC 1 cut(s) 451
BisI GCNGC 3 cut(s) 15, 261, 759
BlsI GCNGC 3 cut(s) 16, 262, 760
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 149, 246
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 211, 242
BmiI GGNNCC 4 cut(s) 430, 459, 717, 806
BmrFI CCNGG 2 cut(s) 149, 246
BmsI GCATC 2 cut(s) 247, 638
BpiI GAAGAC 1 cut(s) 677
Bpu10I CCTNAGC 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 711
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 453
Bse1I ACTGG 1 cut(s) 329
Bse21I CCTNAGG 1 cut(s) 452
BseBI CCWGG 2 cut(s) 149, 246
BseDI CCNNGG 1 cut(s) 711
BseGI GGATG 2 cut(s) 283, 391
BseLI CCNNNNNNNGG 2 cut(s) 310, 453
BseMII CTCAG 3 cut(s) 20, 198, 255
BseNI ACTGG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 26, 247
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 4 cut(s) 142, 212, 303, 328
BslFI GGGAC 2 cut(s) 545, 692
BslI CCNNNNNNNGG 2 cut(s) 310, 453
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 545, 692
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 4 cut(s) 142, 212, 303, 328
Bso31I GGTCTC 1 cut(s) 69
Bsp143I GATC 2 cut(s) 403, 428
BspACI CCGC 3 cut(s) 127, 383, 759
BspANI GGCC 4 cut(s) 142, 212, 303, 328
BspCNI CTCAG 3 cut(s) 19, 199, 256
BspFNI CGCG 1 cut(s) 562
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 4 cut(s) 430, 459, 717, 806
BspMAI CTGCAG 1 cut(s) 474
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 2 cut(s) 423, 436
BspTNI GGTCTC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 711
BssMI GATC 2 cut(s) 403, 428
BssT1I CCWWGG 1 cut(s) 711
Bst2UI CCWGG 2 cut(s) 149, 246
Bst4CI ACNGT 3 cut(s) 412, 536, 554
Bst6I CTCTTC 2 cut(s) 175, 792
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 131, 140, 354
BstDEI CTNAG 5 cut(s) 6, 207, 264, 452, 506
BstF5I GGATG 2 cut(s) 283, 391
BstFNI CGCG 1 cut(s) 562
BstKTI GATC 2 cut(s) 406, 431
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 403, 428
BstMWI GCNNNNNNNGC 3 cut(s) 11, 135, 139
BstNI CCWGG 2 cut(s) 149, 246
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 2 cut(s) 147, 244
BstSFI CTRYAG 2 cut(s) 322, 470
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 2 cut(s) 26, 247
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 4 cut(s) 142, 212, 303, 328
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 283, 391
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 633, 746
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 3 cut(s) 131, 140, 354
CciI TCATGA 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 211, 242
Csp6I GTAC 3 cut(s) 389, 550, 599
CviAII CATG 5 cut(s) 251, 373, 584, 639, 837
CviQI GTAC 3 cut(s) 389, 550, 599
DdeI CTNAG 5 cut(s) 6, 207, 264, 452, 506
DpnI GATC 2 cut(s) 405, 430
DpnII GATC 2 cut(s) 403, 428
Eam1104I CTCTTC 2 cut(s) 175, 792
EarI CTCTTC 2 cut(s) 175, 792
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 711
Eco147I AGGCCT 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 813, 816
Eco81I CCTNAGG 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 147, 244
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 711
FaeI CATG 5 cut(s) 254, 376, 587, 642, 840
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 2 cut(s) 545, 692
FatI CATG 5 cut(s) 250, 372, 583, 638, 836
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 15, 261, 759
FokI GGATG 2 cut(s) 290, 398
Fsp4HI GCNGC 3 cut(s) 15, 261, 759
GluI GCNGC 3 cut(s) 15, 261, 759
HaeIII GGCC 4 cut(s) 142, 212, 303, 328
Hin1II CATG 5 cut(s) 254, 376, 587, 642, 840
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 4 cut(s) 299, 408, 669, 832
Hpy188III TCNNGA 3 cut(s) 251, 530, 794
Hpy8I GTNNAC 1 cut(s) 99
HpyAV CCTTC 3 cut(s) 373, 442, 834
HpyCH4III ACNGT 3 cut(s) 412, 536, 554
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 5 cut(s) 14, 472, 638, 644, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 135, 139
HpyF3I CTNAG 5 cut(s) 6, 207, 264, 452, 506
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 5 cut(s) 254, 376, 587, 642, 840
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 2 cut(s) 403, 428
LmnI GCTCC 2 cut(s) 721, 810
Lsp1109I GCAGC 2 cut(s) 26, 247
LweI GCATC 2 cut(s) 247, 638
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 631
MalI GATC 2 cut(s) 405, 430
MboI GATC 2 cut(s) 403, 428
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 1 cut(s) 428
MluCI AATT 4 cut(s) 415, 492, 594, 762
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 43, 202, 229, 267, 293, 314, 673
MseI TTAA 6 cut(s) 30, 42, 84, 333, 495, 765
MslI CAYNNNNRTG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 149, 246
MunI CAATTG 1 cut(s) 594
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 2 cut(s) 149, 246
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 3 cut(s) 11, 135, 139
NdeII GATC 2 cut(s) 403, 428
NlaIII CATG 5 cut(s) 254, 376, 587, 642, 840
NlaIV GGNNCC 4 cut(s) 430, 459, 717, 806
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 1 cut(s) 642
PagI TCATGA 1 cut(s) 250
PceI AGGCCT 1 cut(s) 303
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 16, 262, 760
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 2 cut(s) 147, 244
PspGI CCWGG 2 cut(s) 147, 244
PspN4I GGNNCC 4 cut(s) 430, 459, 717, 806
PspPI GGNCC 2 cut(s) 211, 242
PstI CTGCAG 1 cut(s) 474
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 3 cut(s) 390, 551, 600
RsaNI GTAC 3 cut(s) 389, 550, 599
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 6 cut(s) 30, 42, 84, 333, 495, 765
SatI GCNGC 3 cut(s) 15, 261, 759
Sau3AI GATC 2 cut(s) 403, 428
Sau96I GGNCC 2 cut(s) 211, 242
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 149, 246
SetI ASST 6 cut(s) 122, 150, 445, 604, 667, 699
SfaNI GCATC 2 cut(s) 247, 638
SfcI CTRYAG 2 cut(s) 322, 470
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 4 cut(s) 415, 492, 594, 762
SseBI AGGCCT 1 cut(s) 303
SsiI CCGC 3 cut(s) 127, 383, 759
StuI AGGCCT 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 147, 244
StyI CCWWGG 1 cut(s) 711
TaaI ACNGT 3 cut(s) 412, 536, 554
TaiI ACGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 656
TasI AATT 4 cut(s) 415, 492, 594, 762
TatI WGTACW 2 cut(s) 388, 549
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 6 cut(s) 30, 42, 84, 333, 495, 765
Tru9I TTAA 6 cut(s) 30, 42, 84, 333, 495, 765
TscAI CASTG 2 cut(s) 640, 753
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 2 cut(s) 14, 260
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 2 cut(s) 197, 707
TspRI CASTG 2 cut(s) 640, 753
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 415
XceI RCATGY 1 cut(s) 642
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.