Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
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| Species | Orthologous Gene IDs |
|---|---|
| prunus_persica | Prupe.7G225400_v2.0.a1 |
| pyrus_communis | pycom09g14340 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 2 cut(s) | 13, 264 |
| AciI | CCGC | 1 cut(s) | 20 |
| AclWI | GGATC | 1 cut(s) | 158 |
| AgsI | TTSAA | 1 cut(s) | 44 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 106 |
| AluBI | AGCT | 3 cut(s) | 82, 172, 338 |
| AluI | AGCT | 3 cut(s) | 82, 172, 338 |
| Alw26I | GTCTC | 1 cut(s) | 293 |
| AlwI | GGATC | 1 cut(s) | 158 |
| AoxI | GGCC | 1 cut(s) | 12 |
| ApeKI | GCWGC | 3 cut(s) | 245, 326, 329 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 48 |
| AspS9I | GGNCC | 2 cut(s) | 100, 223 |
| AsuHPI | GGTGA | 1 cut(s) | 229 |
| AvaII | GGWCC | 2 cut(s) | 100, 223 |
| BbsI | GAAGAC | 1 cut(s) | 203 |
| BbvI | GCAGC | 2 cut(s) | 232, 338 |
| BcoDI | GTCTC | 1 cut(s) | 293 |
| BfaI | CTAG | 1 cut(s) | 227 |
| BfuAI | ACCTGC | 2 cut(s) | 13, 264 |
| BisI | GCNGC | 3 cut(s) | 246, 327, 330 |
| BlsI | GCNGC | 3 cut(s) | 247, 328, 331 |
| Bme18I | GGWCC | 2 cut(s) | 100, 223 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 106 |
| BmgT120I | GGNCC | 2 cut(s) | 100, 223 |
| BpiI | GAAGAC | 1 cut(s) | 203 |
| BpuEI | CTTGAG | 1 cut(s) | 104 |
| BsaJI | CCNNGG | 1 cut(s) | 69 |
| BseDI | CCNNGG | 1 cut(s) | 69 |
| BseMII | CTCAG | 1 cut(s) | 108 |
| BseXI | GCAGC | 2 cut(s) | 232, 338 |
| BshFI | GGCC | 1 cut(s) | 14 |
| BsmAI | GTCTC | 1 cut(s) | 293 |
| BsnI | GGCC | 1 cut(s) | 14 |
| Bsp143I | GATC | 1 cut(s) | 150 |
| BspACI | CCGC | 1 cut(s) | 20 |
| BspANI | GGCC | 1 cut(s) | 14 |
| BspCNI | CTCAG | 1 cut(s) | 109 |
| BspMI | ACCTGC | 2 cut(s) | 13, 264 |
| BspPI | GGATC | 1 cut(s) | 158 |
| BssECI | CCNNGG | 1 cut(s) | 69 |
| BssMI | GATC | 1 cut(s) | 150 |
| BssT1I | CCWWGG | 1 cut(s) | 69 |
| Bst4CI | ACNGT | 2 cut(s) | 241, 304 |
| BstC8I | GCNNGC | 3 cut(s) | 16, 20, 174 |
| BstDEI | CTNAG | 1 cut(s) | 117 |
| BstKTI | GATC | 1 cut(s) | 153 |
| BstMAI | GTCTC | 1 cut(s) | 293 |
| BstMBI | GATC | 1 cut(s) | 150 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 88, 169, 326, 335 |
| BstV1I | GCAGC | 2 cut(s) | 232, 338 |
| BstV2I | GAAGAC | 1 cut(s) | 203 |
| BstX2I | RGATCY | 1 cut(s) | 150 |
| BstYI | RGATCY | 1 cut(s) | 150 |
| BsuRI | GGCC | 1 cut(s) | 14 |
| BveI | ACCTGC | 2 cut(s) | 13, 264 |
| Cac8I | GCNNGC | 3 cut(s) | 16, 20, 174 |
| Cfr13I | GGNCC | 2 cut(s) | 100, 223 |
| CseI | GACGC | 1 cut(s) | 320 |
| CviJI | RGCY | 7 cut(s) | 14, 82, 115, 163, 172, 332, 338 |
| CviKI_1 | RGCY | 7 cut(s) | 14, 82, 115, 163, 172, 332, 338 |
| DdeI | CTNAG | 1 cut(s) | 117 |
| DpnI | GATC | 1 cut(s) | 152 |
| DpnII | GATC | 1 cut(s) | 150 |
| DriI | GACNNNNNGTC | 1 cut(s) | 106 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 106 |
| Eco130I | CCWWGG | 1 cut(s) | 69 |
| Eco47I | GGWCC | 2 cut(s) | 100, 223 |
| EcoO109I | RGGNCCY | 2 cut(s) | 100, 223 |
| EcoT14I | CCWWGG | 1 cut(s) | 69 |
| ErhI | CCWWGG | 1 cut(s) | 69 |
| FauI | CCCGC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 3 cut(s) | 246, 327, 330 |
| Fsp4HI | GCNGC | 3 cut(s) | 246, 327, 330 |
| FspBI | CTAG | 1 cut(s) | 227 |
| GluI | GCNGC | 3 cut(s) | 246, 327, 330 |
| HaeIII | GGCC | 1 cut(s) | 14 |
| HgaI | GACGC | 1 cut(s) | 320 |
| HindIII | AAGCTT | 1 cut(s) | 336 |
| HphI | GGTGA | 1 cut(s) | 229 |
| Hpy166II | GTNNAC | 1 cut(s) | 288 |
| Hpy188III | TCNNGA | 1 cut(s) | 53 |
| Hpy8I | GTNNAC | 1 cut(s) | 288 |
| HpyAV | CCTTC | 2 cut(s) | 113, 214 |
| HpyCH4III | ACNGT | 2 cut(s) | 241, 304 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 28 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 88, 169, 326, 335 |
| HpyF3I | CTNAG | 1 cut(s) | 117 |
| Kzo9I | GATC | 1 cut(s) | 150 |
| LmnI | GCTCC | 1 cut(s) | 169 |
| LpnPI | CCDG | 6 cut(s) | 8, 28, 66, 129, 200, 259 |
| Lsp1109I | GCAGC | 2 cut(s) | 232, 338 |
| MaeI | CTAG | 1 cut(s) | 227 |
| MaeIII | GTNAC | 1 cut(s) | 64 |
| MalI | GATC | 1 cut(s) | 152 |
| MboI | GATC | 1 cut(s) | 150 |
| MboII | GAAGA | 2 cut(s) | 56, 203 |
| MflI | RGATCY | 1 cut(s) | 150 |
| MluCI | AATT | 2 cut(s) | 29, 135 |
| MnlI | CCTC | 7 cut(s) | 81, 87, 91, 136, 141, 153, 301 |
| MroXI | GAANNNNTTC | 1 cut(s) | 48 |
| MseI | TTAA | 1 cut(s) | 264 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 88, 169, 326, 335 |
| NdeII | GATC | 1 cut(s) | 150 |
| PaqCI | CACCTGC | 1 cut(s) | 13 |
| PdmI | GAANNNNTTC | 1 cut(s) | 48 |
| PkrI | GCNGC | 3 cut(s) | 247, 328, 331 |
| PpuMI | RGGWCCY | 2 cut(s) | 100, 223 |
| Psp5II | RGGWCCY | 2 cut(s) | 100, 223 |
| PspPI | GGNCC | 2 cut(s) | 100, 223 |
| PspPPI | RGGWCCY | 2 cut(s) | 100, 223 |
| PsuI | RGATCY | 1 cut(s) | 150 |
| SaqAI | TTAA | 1 cut(s) | 264 |
| SatI | GCNGC | 3 cut(s) | 246, 327, 330 |
| Sau3AI | GATC | 1 cut(s) | 150 |
| Sau96I | GGNCC | 2 cut(s) | 100, 223 |
| SetI | ASST | 8 cut(s) | 27, 84, 105, 174, 193, 228, 278, 340 |
| SinI | GGWCC | 2 cut(s) | 100, 223 |
| SmlI | CTYRAG | 1 cut(s) | 83 |
| SmoI | CTYRAG | 1 cut(s) | 83 |
| Sse9I | AATT | 2 cut(s) | 29, 135 |
| SsiI | CCGC | 1 cut(s) | 20 |
| SspI | AATATT | 1 cut(s) | 262 |
| SspMI | CTAG | 1 cut(s) | 227 |
| StyI | CCWWGG | 1 cut(s) | 69 |
| TaaI | ACNGT | 2 cut(s) | 241, 304 |
| TasI | AATT | 2 cut(s) | 29, 135 |
| Tru1I | TTAA | 1 cut(s) | 264 |
| Tru9I | TTAA | 1 cut(s) | 264 |
| TseI | GCWGC | 3 cut(s) | 245, 326, 329 |
| TspDTI | ATGAA | 1 cut(s) | 184 |
| VpaK11BI | GGWCC | 2 cut(s) | 100, 223 |
| XmnI | GAANNNNTTC | 1 cut(s) | 48 |
| XspI | CTAG | 1 cut(s) | 227 |