pycom09g14340

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0029085
Physical Location & Seq
Forward (+)
13808329 .. 13809069
741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14340.2

Sequence Viewer

Length: 657 bp
ATGATTTACAATAGGTCGAGGAAAATAAGTAGCATAAGGAAGCTTATATGGAAACTAACGAAAATGTATGTGGGTTTCCTCCACCACCAGAACTTGGTTTATGATCGGACAATGCAAGTCGTGGCCTGCCCTCAGGTGCAATATCTATCCATCAAAGCCATGCCGGAGAGCATTGTACACAAGGAAAAAGAGCTTGAGAAGCTGAGGGAGGACCTTCTATCCAAACCCGAGAACCTTAGGGAGAGAATTGTGGATAGGAGGATCTCAAAGAGGCTTAGGGAGCTGTCTCTTTTAGAGCAACCTTTCATTAAAGATGATAGCCTTTTGGTGAAAAACATAGTGAAGCAGATCGTAGCTGCTTTTGGTGAGAACATAAAAAGTCAAAGATATTATCAACCTTTGAGTGTGGAGGGTACTCAATTAGATATGAGTGTAGTGTTTCAGTTCATACTTTTTTCCACATTCCATGTTTCTTGCTTGAAAAAGCATTTGGGACCACAAGTAACAACCACAGTGCCGCTGCCAGTTATCATAGATGATAGGATTTTGCAAAGATGTACCAGGTGCAATCTTGGACAGAAGAATGATCAAGAAAGTGCTACAGCTACGGAAGTCTTAGTTCAATGGCAAAACCACTCCAAAGATGATGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF_TS S1

Protein Analysis

219

Amino Acids

25.44

Weight (kDa)

9.43

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029085)

Species Orthologous Gene IDs
prunus_persica Prupe.7G225400_v2.0.a1
pyrus_communis pycom09g14340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AciI CCGC 1 cut(s) 518
AclWI GGATC 1 cut(s) 269
AfaI GTAC 3 cut(s) 177, 415, 559
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 481, 623
AjnI CCWGG 1 cut(s) 560
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 6 cut(s) 43, 193, 202, 283, 356, 605
AluI AGCT 6 cut(s) 43, 193, 202, 283, 356, 605
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 356, 520
AspS9I GGNCC 2 cut(s) 211, 494
AsuHPI GGTGA 2 cut(s) 340, 377
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 2 cut(s) 211, 494
AxyI CCTNAGG 2 cut(s) 132, 236
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 2 cut(s) 343, 507
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 562
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 600
BisI GCNGC 3 cut(s) 357, 518, 521
BlsI GCNGC 3 cut(s) 358, 519, 522
Bme1390I CCNGG 1 cut(s) 562
Bme18I GGWCC 2 cut(s) 211, 494
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 211, 494
BmiI GGNNCC 1 cut(s) 495
BmrFI CCNGG 1 cut(s) 562
BmsI GCATC 1 cut(s) 637
Bpu10I CCTNAGC 2 cut(s) 203, 275
BpuEI CTTGAG 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 524
Bse21I CCTNAGG 2 cut(s) 132, 236
BseBI CCWGG 1 cut(s) 562
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMII CTCAG 2 cut(s) 146, 194
BseNI ACTGG 1 cut(s) 524
BseXI GCAGC 2 cut(s) 343, 507
BshFI GGCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 4 cut(s) 103, 261, 348, 586
BspACI CCGC 1 cut(s) 518
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 2 cut(s) 145, 195
BspLI GGNNCC 1 cut(s) 495
BspPI GGATC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 175
BsrI ACTGG 1 cut(s) 524
BssMI GATC 4 cut(s) 103, 261, 348, 586
Bst2UI CCWGG 1 cut(s) 562
Bst4CI ACNGT 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 5 cut(s) 132, 203, 236, 275, 616
BstKTI GATC 4 cut(s) 106, 264, 351, 589
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 4 cut(s) 103, 261, 348, 586
BstMWI GCNNNNNNNGC 2 cut(s) 199, 280
BstNI CCWGG 1 cut(s) 562
BstSCI CCNGG 1 cut(s) 560
BstSFI CTRYAG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 343, 507
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
Bsu36I CCTNAGG 2 cut(s) 132, 236
BsuRI GGCC 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 211, 494
CsiI ACCWGGT 1 cut(s) 560
Csp6I GTAC 3 cut(s) 176, 414, 558
CviAII CATG 3 cut(s) 160, 467, 654
CviQI GTAC 3 cut(s) 176, 414, 558
DdeI CTNAG 5 cut(s) 132, 203, 236, 275, 616
DpnI GATC 4 cut(s) 105, 263, 350, 588
DpnII GATC 4 cut(s) 103, 261, 348, 586
Eco47I GGWCC 2 cut(s) 211, 494
Eco81I CCTNAGG 2 cut(s) 132, 236
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 560
FaeI CATG 3 cut(s) 163, 470, 657
FaqI GGGAC 1 cut(s) 507
FatI CATG 3 cut(s) 159, 466, 653
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 357, 518, 521
Fsp4HI GCNGC 3 cut(s) 357, 518, 521
GluI GCNGC 3 cut(s) 357, 518, 521
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 164
Hin1II CATG 3 cut(s) 163, 470, 657
HindIII AAGCTT 1 cut(s) 41
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 340, 377
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 1 cut(s) 590
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4V TGCA 4 cut(s) 115, 139, 550, 567
HpyF10VI GCNNNNNNNGC 2 cut(s) 199, 280
HpyF3I CTNAG 5 cut(s) 132, 203, 236, 275, 616
Hsp92II CATG 3 cut(s) 163, 470, 657
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 4 cut(s) 103, 261, 348, 586
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 7 cut(s) 101, 119, 139, 177, 537, 547, 574
Lsp1109I GCAGC 2 cut(s) 343, 507
LweI GCATC 1 cut(s) 637
MabI ACCWGGT 1 cut(s) 560
MaeIII GTNAC 1 cut(s) 502
MalI GATC 4 cut(s) 105, 263, 350, 588
MboI GATC 4 cut(s) 103, 261, 348, 586
MboII GAAGA 1 cut(s) 592
MflI RGATCY 1 cut(s) 261
MluCI AATT 2 cut(s) 246, 419
MnlI CCTC 8 cut(s) 12, 89, 141, 198, 202, 252, 264, 403
MseI TTAA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 562
MvaI CCWGG 1 cut(s) 562
MwoI GCNNNNNNNGC 2 cut(s) 199, 280
NdeII GATC 4 cut(s) 103, 261, 348, 586
NlaIII CATG 3 cut(s) 163, 470, 657
NlaIV GGNNCC 1 cut(s) 495
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 3 cut(s) 358, 519, 522
PpuMI RGGWCCY 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 1 cut(s) 495
PspPI GGNCC 2 cut(s) 211, 494
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 3 cut(s) 177, 415, 559
RsaNI GTAC 3 cut(s) 176, 414, 558
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 3 cut(s) 357, 518, 521
Sau3AI GATC 4 cut(s) 103, 261, 348, 586
Sau96I GGNCC 2 cut(s) 211, 494
ScrFI CCNGG 1 cut(s) 562
SexAI ACCWGGT 1 cut(s) 560
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 600
SinI GGWCC 2 cut(s) 211, 494
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 2 cut(s) 246, 419
SsiI CCGC 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 560
TaaI ACNGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 17
TasI AATT 2 cut(s) 246, 419
TatI WGTACW 1 cut(s) 175
TauI GCSGC 1 cut(s) 520
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 519
TseI GCWGC 2 cut(s) 356, 520
TspDTI ATGAA 2 cut(s) 295, 436
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 519
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 211, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.