Prupe.8G260200_v2.0.a1

microtubule motor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0018120
Physical Location & Seq
Reverse (-)
22021459 .. 22027678
6220 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G260200.6

Sequence Viewer

Length: 1320 bp
ATGCTTCGACCACCACCACATCAGACTGCTGCTCGAGGGCGCAAGAAGACACATCTGACTGCCGCCTCCGAGAGTCCCTCTCTCTCTCTCCCACCACGAGGGTCAACGACACCTCGGGTAATCGACCACCACCTCCAAGGGTCGACATCCACCTCTCCCTCTCTCAGCTCCTCTCACCACCGCCTCCAAGGGTCGAGTTCTGCCTCTTCCTCCCTCAGCTCCTCGCTCTCTCTCCCACAACGAAGCTCAACCATCACCGGTCAGATCATCGTCTTTGAGTTCCACGACGTAGATGAAGTGGGTTGTGAAGCCAGTAAACATCTAGCCATGGATGATAATCAGTTCATGGTTGTGATGAGTAAGACATCGGTCACACAAAGCAACACGATGATACCTAAAACATCTGTGGAAAAGATTCAACTTGACAAACTTTGTACAAGTTGCGAACCGTTTTTCCCTCCACCTCCTAATGTAAAAAAAAAGTTGAGTACTGTAAGATTTGGAATGTTCACCCTCAGAATCAGTAGAAAAATATGCCTGGGTGAATGGGGGAGTAAAAAATGGCGAGTAGAAATTAAAAATGAATTAGCACACCGGTGTAGAACAACTATAGGTAGGGAGTGGCTCACTTTTGTGAGAGAGAACCAACTTCTAGTTGGTGACCATGTTGTTTTCCGGGTCCTTCCCTCCACTATGGGGTTGGTGCTGGTTGGCGTTTATCGCCACAACCCAGCTACTCCTCCCCGTGCTTCCTCCAGTGTTCGTCCACATGAGGACCAAGACCTGTCGGAGGTGGTCCAGAAGCTTAACGATGTGATAAAGCTTCGTAATGAGGAGTTGGAAGCGAAGGAGGAAACTATAAGGAAACTTCGTAATGAGGAGTTGGAAGCGAAGGAGGAAACTATAAGGAAACTAAGTGAAAGATTGTTATCGGAACTAGGTGAAAGATTGTTATTGGAGCAAAGGATTTCTGGACTTGTGAAAAGTCAAGCCGATAAGAATGATTTTCTAGAGAAAAAAATTCAGCAAGAATTCGCTGTTGCTCATTCAACTCTTGCCATCATGAACTCTGAGATTGCTTCATTGGAAAGTAACTTAAAGGACATCGATAGAAACCATCAACTCATCTTGAGGACGCAGTGGGATCAATTAGCTGAGTTGGAAGTGGTTGTCAAAGAGGAACAACTCATGAGGGAGCGCTTATTTAATACTATAGAAGGTTCGCATCAATTGATTGCTAATGATCCAACCGAAAATGTTGCTTCAAGAGGTGAAGATCAAGAACCTGCAACTTTGCCCTCAAGGCCTGATACATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04914 map04914
Pfam Domains
Protein Families
DUF760 Kinesin Microtub_bd

Protein Analysis

440

Amino Acids

49.43

Weight (kDa)

7.27

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1294
AccI GTMKAC 1 cut(s) 143
AciI CCGC 2 cut(s) 63, 181
AclWI GGATC 2 cut(s) 1152, 1238
AcsI RAATTY 2 cut(s) 1020, 1031
AfaI GTAC 2 cut(s) 436, 490
AfeI AGCGCT 1 cut(s) 1199
AfiI CCNNNNNNNGG 3 cut(s) 98, 696, 790
AgeI ACCGGT 2 cut(s) 257, 594
AgsI TTSAA 3 cut(s) 419, 1050, 1266
AjnI CCWGG 1 cut(s) 537
AleI CACNNNNGTG 2 cut(s) 595, 632
AloI GAACNNNNNNTCC 2 cut(s) 438, 470
AluBI AGCT 7 cut(s) 168, 219, 246, 734, 805, 823, 1154
AluI AGCT 7 cut(s) 168, 219, 246, 734, 805, 823, 1154
AlwI GGATC 2 cut(s) 1152, 1238
Ama87I CYCGRG 2 cut(s) 33, 114
Aor51HI AGCGCT 1 cut(s) 1199
AoxI GGCC 1 cut(s) 1304
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 1020, 1031
AsiGI ACCGGT 2 cut(s) 257, 594
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 2 cut(s) 42, 1200
AspS9I GGNCC 3 cut(s) 679, 775, 796
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 7 cut(s) 167, 247, 502, 554, 671, 953, 1283
AvaI CYCGRG 2 cut(s) 33, 114
AvaII GGWCC 3 cut(s) 679, 775, 796
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 53
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 260, 1067, 1125
BcgI CGANNNNNNTGC 2 cut(s) 1241, 1275
BciT130I CCWGG 1 cut(s) 539
BcnI CCSGG 1 cut(s) 677
BfaI CTAG 4 cut(s) 323, 653, 938, 1010
BfmI CTRYAG 2 cut(s) 609, 1212
BfoI RGCGCY 1 cut(s) 1201
BfuAI ACCTGC 1 cut(s) 1294
BglI GCCNNNNNGGC 1 cut(s) 1303
BisI GCNGC 2 cut(s) 30, 63
BlsI GCNGC 2 cut(s) 31, 64
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 2 cut(s) 539, 677
Bme18I GGWCC 3 cut(s) 679, 775, 796
BmeT110I CYCGRG 2 cut(s) 33, 114
BmgT120I GGNCC 3 cut(s) 679, 775, 796
BmiI GGNNCC 1 cut(s) 680
BmrFI CCNGG 2 cut(s) 539, 677
BmsI GCATC 1 cut(s) 1234
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 53
BplI GAGNNNNNCTC 4 cut(s) 62, 94, 64, 96
BpmI CTGGAG 1 cut(s) 739
Bpu10I CCTNAGC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 1150, 1285
BpuMI CCSGG 1 cut(s) 677
Bsa29I ATCGAT 1 cut(s) 1107
BsaBI GATNNNNATC 2 cut(s) 336, 928
BsaJI CCNNGG 5 cut(s) 113, 136, 187, 327, 538
BsaWI WCCGGW 2 cut(s) 257, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 696, 790
Bse118I RCCGGY 2 cut(s) 257, 594
Bse1I ACTGG 2 cut(s) 312, 756
Bse8I GATNNNNATC 2 cut(s) 336, 928
BseBI CCWGG 1 cut(s) 539
BseCI ATCGAT 1 cut(s) 1107
BseDI CCNNGG 5 cut(s) 113, 136, 187, 327, 538
BseGI GGATG 2 cut(s) 146, 337
BseJI GATNNNNATC 2 cut(s) 336, 928
BseLI CCNNNNNNNGG 3 cut(s) 98, 696, 790
BseMII CTCAG 5 cut(s) 178, 229, 529, 1062, 1146
BseNI ACTGG 2 cut(s) 312, 756
BseRI GAGGAG 5 cut(s) 160, 211, 729, 848, 893
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 730
BshFI GGCC 1 cut(s) 1306
BshTI ACCGGT 2 cut(s) 257, 594
BshVI ATCGAT 1 cut(s) 1107
BsiHKCI CYCGRG 2 cut(s) 33, 114
BsiSI CCGG 3 cut(s) 258, 595, 676
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 3 cut(s) 98, 696, 790
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 1306
BsoBI CYCGRG 2 cut(s) 33, 114
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 4 cut(s) 264, 1144, 1243, 1276
Bsp19I CCATGG 1 cut(s) 327
BspACI CCGC 2 cut(s) 63, 181
BspANI GGCC 1 cut(s) 1306
BspCNI CTCAG 5 cut(s) 177, 228, 528, 1063, 1147
BspDI ATCGAT 1 cut(s) 1107
BspHI TCATGA 2 cut(s) 1062, 1188
BspLI GGNNCC 1 cut(s) 680
BspMI ACCTGC 1 cut(s) 1294
BspPI GGATC 2 cut(s) 1152, 1238
BsrFI RCCGGY 2 cut(s) 257, 594
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 2 cut(s) 312, 756
BssAI RCCGGY 2 cut(s) 257, 594
BssECI CCNNGG 5 cut(s) 113, 136, 187, 327, 538
BssMI GATC 4 cut(s) 264, 1144, 1243, 1276
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 3 cut(s) 136, 187, 327
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 2 cut(s) 450, 493
Bst6I CTCTTC 1 cut(s) 211
BstAUI TGTACA 1 cut(s) 434
BstDEI CTNAG 6 cut(s) 164, 215, 515, 914, 1071, 1155
BstDSI CCRYGG 1 cut(s) 327
BstEII GGTNACC 1 cut(s) 659
BstENI CCTNNNNNAGG 1 cut(s) 788
BstF5I GGATG 2 cut(s) 146, 337
BstH2I RGCGCY 1 cut(s) 1201
BstHHI GCGC 2 cut(s) 42, 1200
BstKTI GATC 4 cut(s) 267, 1147, 1246, 1279
BstMBI GATC 4 cut(s) 264, 1144, 1243, 1276
BstMWI GCNNNNNNNGC 2 cut(s) 720, 1303
BstNI CCWGG 1 cut(s) 539
BstPAI GACNNNNGTC 1 cut(s) 368
BstPI GGTNACC 1 cut(s) 659
BstSCI CCNGG 2 cut(s) 537, 675
BstSFI CTRYAG 2 cut(s) 609, 1212
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 1107
BsuRI GGCC 1 cut(s) 1306
BsuTUI ATCGAT 1 cut(s) 1107
BtgI CCRYGG 1 cut(s) 327
BtsCI GGATG 2 cut(s) 146, 337
BtsI GCAGTG 1 cut(s) 1145
BtsIMutI CAGTG 2 cut(s) 763, 1145
BveI ACCTGC 1 cut(s) 1294
CciI TCATGA 2 cut(s) 1062, 1188
CfoI GCGC 2 cut(s) 42, 1200
Cfr10I RCCGGY 2 cut(s) 257, 594
Cfr13I GGNCC 3 cut(s) 679, 775, 796
ClaI ATCGAT 1 cut(s) 1107
CseI GACGC 1 cut(s) 1144
Csp6I GTAC 2 cut(s) 435, 489
CspAI ACCGGT 2 cut(s) 257, 594
CviAII CATG 6 cut(s) 328, 346, 665, 770, 1063, 1189
CviQI GTAC 2 cut(s) 435, 489
DdeI CTNAG 6 cut(s) 164, 215, 515, 914, 1071, 1155
DpnI GATC 4 cut(s) 266, 1146, 1245, 1278
DpnII GATC 4 cut(s) 264, 1144, 1243, 1276
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 136, 187, 327
Eco147I AGGCCT 1 cut(s) 1306
Eco47I GGWCC 3 cut(s) 679, 775, 796
Eco47III AGCGCT 1 cut(s) 1199
Eco88I CYCGRG 2 cut(s) 33, 114
Eco91I GGTNACC 1 cut(s) 659
EcoNI CCTNNNNNAGG 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 679
EcoO65I GGTNACC 1 cut(s) 659
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 136, 187, 327
ErhI CCWWGG 3 cut(s) 136, 187, 327
FaeI CATG 6 cut(s) 331, 349, 668, 773, 1066, 1192
FaqI GGGAC 1 cut(s) 60
FatI CATG 6 cut(s) 327, 345, 664, 769, 1062, 1188
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 30, 63
FokI GGATG 2 cut(s) 133, 344
Fsp4HI GCNGC 2 cut(s) 30, 63
FspBI CTAG 4 cut(s) 323, 653, 938, 1010
GlaI GCGC 2 cut(s) 41, 1199
GluI GCNGC 2 cut(s) 30, 63
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 739
HaeII RGCGCY 1 cut(s) 1201
HaeIII GGCC 1 cut(s) 1306
HapII CCGG 3 cut(s) 258, 595, 676
HgaI GACGC 1 cut(s) 1144
HhaI GCGC 2 cut(s) 42, 1200
Hin1II CATG 6 cut(s) 331, 349, 668, 773, 1066, 1192
Hin6I GCGC 2 cut(s) 40, 1198
HinP1I GCGC 2 cut(s) 40, 1198
HincII GTYRAC 2 cut(s) 105, 144
HindII GTYRAC 2 cut(s) 105, 144
HindIII AAGCTT 2 cut(s) 803, 821
HinfI GANTC 3 cut(s) 73, 415, 519
HpaII CCGG 3 cut(s) 258, 595, 676
HphI GGTGA 7 cut(s) 167, 247, 502, 554, 671, 953, 1283
Hpy166II GTNNAC 5 cut(s) 105, 144, 317, 510, 767
Hpy188I TCNGA 8 cut(s) 24, 57, 70, 264, 518, 790, 934, 1072
Hpy188III TCNNGA 8 cut(s) 799, 972, 1010, 1063, 1129, 1189, 1266, 1280
Hpy8I GTNNAC 5 cut(s) 105, 144, 317, 510, 767
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 4 cut(s) 692, 841, 886, 1211
HpyCH4III ACNGT 2 cut(s) 450, 493
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 1 cut(s) 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 720, 1303
HpyF3I CTNAG 6 cut(s) 164, 215, 515, 914, 1071, 1155
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 6 cut(s) 331, 349, 668, 773, 1066, 1192
HspAI GCGC 2 cut(s) 40, 1198
Kzo9I GATC 4 cut(s) 264, 1144, 1243, 1276
LmnI GCTCC 4 cut(s) 173, 224, 958, 1195
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 1234
MaeI CTAG 4 cut(s) 323, 653, 938, 1010
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 370, 659, 1091
MalI GATC 4 cut(s) 266, 1146, 1245, 1278
MboI GATC 4 cut(s) 264, 1144, 1243, 1276
MboII GAAGA 3 cut(s) 58, 198, 1286
MfeI CAATTG 1 cut(s) 1229
MluCI AATT 6 cut(s) 573, 584, 1020, 1031, 1148, 1229
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 5 cut(s) 768, 819, 864, 1140, 1271
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 4 cut(s) 576, 807, 1097, 1206
MslI CAYNNNNRTG 3 cut(s) 350, 595, 632
MspI CCGG 3 cut(s) 258, 595, 676
MspR9I CCNGG 2 cut(s) 539, 677
MunI CAATTG 1 cut(s) 1229
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 2 cut(s) 720, 1303
NciI CCSGG 1 cut(s) 677
NcoI CCATGG 1 cut(s) 327
NdeII GATC 4 cut(s) 264, 1144, 1243, 1276
NlaIII CATG 6 cut(s) 331, 349, 668, 773, 1066, 1192
NlaIV GGNNCC 1 cut(s) 680
NmuCI GTSAC 2 cut(s) 370, 659
OliI CACNNNNGTG 2 cut(s) 595, 632
PaeR7I CTCGAG 1 cut(s) 33
PagI TCATGA 2 cut(s) 1062, 1188
PceI AGGCCT 1 cut(s) 1306
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 415, 519
PinAI ACCGGT 2 cut(s) 257, 594
PkrI GCNGC 2 cut(s) 31, 64
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PpuMI RGGWCCY 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 1 cut(s) 537
PspEI GGTNACC 1 cut(s) 659
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 680
PspPI GGNCC 3 cut(s) 679, 775, 796
PspPPI RGGWCCY 1 cut(s) 679
PspXI VCTCGAGB 1 cut(s) 33
RsaI GTAC 2 cut(s) 436, 490
RsaNI GTAC 2 cut(s) 435, 489
RseI CAYNNNNRTG 3 cut(s) 350, 595, 632
SalI GTCGAC 1 cut(s) 142
SaqAI TTAA 4 cut(s) 576, 807, 1097, 1206
SatI GCNGC 2 cut(s) 30, 63
Sau3AI GATC 4 cut(s) 264, 1144, 1243, 1276
Sau96I GGNCC 3 cut(s) 679, 775, 796
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 539, 677
SfaNI GCATC 1 cut(s) 1234
SfcI CTRYAG 2 cut(s) 609, 1212
Sfr274I CTCGAG 1 cut(s) 33
SgrAI CRCCGGYG 1 cut(s) 594
SinI GGWCC 3 cut(s) 679, 775, 796
SlaI CTCGAG 1 cut(s) 33
SmiMI CAYNNNNRTG 3 cut(s) 350, 595, 632
SmlI CTYRAG 3 cut(s) 33, 1129, 1300
SmoI CTYRAG 3 cut(s) 33, 1129, 1300
Sse9I AATT 6 cut(s) 573, 584, 1020, 1031, 1148, 1229
SseBI AGGCCT 1 cut(s) 1306
SsiI CCGC 2 cut(s) 63, 181
SspMI CTAG 4 cut(s) 323, 653, 938, 1010
StuI AGGCCT 1 cut(s) 1306
StyD4I CCNGG 2 cut(s) 537, 675
StyI CCWWGG 3 cut(s) 136, 187, 327
TaaI ACNGT 2 cut(s) 450, 493
TaiI ACGT 1 cut(s) 291
TaqI TCGA 6 cut(s) 7, 34, 123, 143, 194, 1107
TaqII GACCGA 1 cut(s) 358
TasI AATT 6 cut(s) 573, 584, 1020, 1031, 1148, 1229
TatI WGTACW 2 cut(s) 434, 488
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 415, 519
Tru1I TTAA 4 cut(s) 576, 807, 1097, 1206
Tru9I TTAA 4 cut(s) 576, 807, 1097, 1206
TscAI CASTG 2 cut(s) 763, 1145
TseFI GTSAC 2 cut(s) 370, 659
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 2 cut(s) 370, 659
TspDTI ATGAA 5 cut(s) 309, 334, 597, 1071, 1079
TspRI CASTG 2 cut(s) 763, 1145
VpaK11BI GGWCC 3 cut(s) 679, 775, 796
XagI CCTNNNNNAGG 1 cut(s) 788
XapI RAATTY 2 cut(s) 1020, 1031
XbaI TCTAGA 1 cut(s) 1009
XcmI CCANNNNNNNNNTGG 2 cut(s) 653, 697
XhoI CTCGAG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 4 cut(s) 323, 653, 938, 1010
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.