Prupe.8G267300_v2.0.a1

Replication protein A interacting middle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0019180
Physical Location & Seq
Forward (+)
22313937 .. 22317753
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G267300.3

Sequence Viewer

Length: 804 bp
ATGGAGGGAGAAGAAGAAGCAGCCGCAGCTTCAAGGAAATCAACAGAAAGAAAGCATTCTCTCAAACCCCACCCTATCTTCAATAACTATAACAGCTGGAAGCACAAGTTGAGAGAGAACTGTTACAAAAGGGTAAGAGAAGACAGAAGGCGTTTGCTTTGGAAGATGAGAATGGTGCCCACTCCACAATTTCTCAGTCACATTCCAAATGATTTGATCAAATCTGCTTTCCAGGACATAGTTTCCGCTGAATTGGGAAAAATTAAGGACTCGTCATTGGATGACAATACGAGGATTTCGAAATCTGTTCCTGAGGGCAATGATGTGTTATGGGAATATGAAGGCCTTACAGACGCATACCAAGGTGATTGTGAAGAGATGCTATTAGAAATGCAAAGGATCTTTTACCAAGATCTGAGGACAGAGCCAACTACAAAAGAAACAGAAATCCGTGTTGAAACATGGGAAGATGAAGAGGATGAGTATTTGGCCCAGGCGGTTTTTGAGCATATGCAACTGAACGACAAGCAGCTGCGCAAGGAGGAAGTTTGGTGCCCCATCTGTAAGCATGGAGTACTACAAGAGAATTATCATCTGATTTACTGCAGCCTATGTGAAATTCAGCTCAACAAAGGCGATGAGGTTAATTTGGAATTATTGAGGGGCAGGCTAGCAGAAGTTCATGCAGAGCATTTTGATCGTGGGTGCACATTGAAGCCCAAGTTTTGCATCGAGACTAGATTTAATATAACTGCATTGTACATTCTTTGTGCTGCTTGTAATACATTTGAGATTGTAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPA_interact_C RPA_interact_M RPA_interact_N

Protein Analysis

268

Amino Acids

31.46

Weight (kDa)

5.33

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12760
malus_domestica MD03G1298900.v1.1 MD11G1318200.v1.1
prunus_persica Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1
pyrus_communis pycom03g23700 pycom11g27740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 536
AccB1I GGYRCC 2 cut(s) 175, 552
AciI CCGC 3 cut(s) 24, 246, 497
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 1 cut(s) 618
AfaI GTAC 2 cut(s) 576, 761
AgsI TTSAA 4 cut(s) 33, 82, 458, 715
AjnI CCWGG 2 cut(s) 231, 492
AluBI AGCT 4 cut(s) 29, 96, 532, 625
AluI AGCT 4 cut(s) 29, 96, 532, 625
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 1 cut(s) 728
Alw44I GTGCAC 1 cut(s) 706
AlwI GGATC 1 cut(s) 407
AoxI GGCC 2 cut(s) 343, 489
ApaLI GTGCAC 1 cut(s) 706
ApeKI GCWGC 6 cut(s) 20, 26, 529, 532, 606, 773
ApoI RAATTY 1 cut(s) 618
Asp700I GAANNNNTTC 1 cut(s) 55
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 1 cut(s) 490
AsuHPI GGTGA 1 cut(s) 377
AsuII TTCGAA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 670
AxyI CCTNAGG 1 cut(s) 312
BaeGI GKGCMC 3 cut(s) 180, 557, 710
BanI GGYRCC 2 cut(s) 175, 552
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 710
BbvI GCAGC 6 cut(s) 32, 38, 519, 541, 618, 760
BccI CCATC 1 cut(s) 566
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 2 cut(s) 233, 494
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 2 cut(s) 671, 738
BfmI CTRYAG 1 cut(s) 604
BglII AGATCT 1 cut(s) 412
BisI GCNGC 7 cut(s) 21, 24, 27, 530, 533, 607, 774
BlsI GCNGC 7 cut(s) 22, 25, 28, 531, 534, 608, 775
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 2 cut(s) 233, 494
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 2 cut(s) 177, 554
BmrFI CCNGG 2 cut(s) 233, 494
BmsI GCATC 2 cut(s) 369, 738
BmtI GCTAGC 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 361, 492
Bse21I CCTNAGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 325
BseBI CCWGG 2 cut(s) 233, 494
BseDI CCNNGG 2 cut(s) 361, 492
BseGI GGATG 2 cut(s) 286, 484
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 208, 303, 407
BseSI GKGCMC 3 cut(s) 180, 557, 710
BseXI GCAGC 6 cut(s) 32, 38, 519, 541, 618, 760
BshFI GGCC 2 cut(s) 345, 491
BshNI GGYRCC 2 cut(s) 175, 552
BsiHKAI GWGCWC 1 cut(s) 710
BsmAI GTCTC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 345, 491
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 180, 557, 710
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 4 cut(s) 216, 399, 412, 697
BspACI CCGC 3 cut(s) 24, 246, 497
BspANI GGCC 2 cut(s) 345, 491
BspCNI CTCAG 3 cut(s) 207, 304, 408
BspLI GGNNCC 2 cut(s) 177, 554
BspMAI CTGCAG 1 cut(s) 608
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 2 cut(s) 175, 552
BsrDI GCAATG 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 759
BssECI CCNNGG 2 cut(s) 361, 492
BssMI GATC 4 cut(s) 216, 399, 412, 697
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 2 cut(s) 233, 494
Bst4CI ACNGT 1 cut(s) 122
Bst6I CTCTTC 2 cut(s) 369, 468
BstAUI TGTACA 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 668, 672
BstDEI CTNAG 3 cut(s) 194, 312, 416
BstF5I GGATG 2 cut(s) 286, 484
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 4 cut(s) 219, 402, 415, 700
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 4 cut(s) 216, 399, 412, 697
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 2 cut(s) 233, 494
BstSCI CCNGG 2 cut(s) 231, 492
BstSFI CTRYAG 1 cut(s) 604
BstSLI GKGCMC 3 cut(s) 180, 557, 710
BstV1I GCAGC 6 cut(s) 32, 38, 519, 541, 618, 760
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 399, 412
BstYI RGATCY 2 cut(s) 399, 412
Bsu36I CCTNAGG 1 cut(s) 312
BsuRI GGCC 2 cut(s) 345, 491
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 2 cut(s) 286, 484
Cac8I GCNNGC 2 cut(s) 668, 672
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 1 cut(s) 490
CseI GACGC 1 cut(s) 362
Csp6I GTAC 2 cut(s) 575, 760
CviAII CATG 3 cut(s) 462, 569, 683
CviQI GTAC 2 cut(s) 575, 760
DdeI CTNAG 3 cut(s) 194, 312, 416
DpnI GATC 4 cut(s) 218, 401, 414, 699
DpnII GATC 4 cut(s) 216, 399, 412, 697
Eam1104I CTCTTC 2 cut(s) 369, 468
EarI CTCTTC 2 cut(s) 369, 468
Eco130I CCWWGG 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 345
Eco81I CCTNAGG 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 231, 492
EcoT14I CCWWGG 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 3 cut(s) 465, 572, 686
FatI CATG 3 cut(s) 461, 568, 682
FauNDI CATATG 1 cut(s) 510
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 7 cut(s) 21, 24, 27, 530, 533, 607, 774
FokI GGATG 2 cut(s) 293, 491
Fsp4HI GCNGC 7 cut(s) 21, 24, 27, 530, 533, 607, 774
FspBI CTAG 2 cut(s) 671, 738
FspI TGCGCA 1 cut(s) 536
GlaI GCGC 1 cut(s) 536
GluI GCNGC 7 cut(s) 21, 24, 27, 530, 533, 607, 774
HaeIII GGCC 2 cut(s) 345, 491
HgaI GACGC 1 cut(s) 362
HhaI GCGC 1 cut(s) 537
Hin1II CATG 3 cut(s) 465, 572, 686
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 1 cut(s) 269
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 708
Hpy188I TCNGA 2 cut(s) 417, 597
Hpy188III TCNNGA 2 cut(s) 311, 733
Hpy8I GTNNAC 1 cut(s) 708
HpyAV CCTTC 2 cut(s) 141, 335
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4V TGCA 7 cut(s) 394, 514, 606, 686, 708, 729, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 3 cut(s) 194, 312, 416
Hsp92II CATG 3 cut(s) 465, 572, 686
HspAI GCGC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 216, 399, 412, 697
LpnPI CCDG 7 cut(s) 82, 218, 245, 324, 479, 506, 652
Lsp1109I GCAGC 6 cut(s) 32, 38, 519, 541, 618, 760
LweI GCATC 2 cut(s) 369, 738
MaeI CTAG 2 cut(s) 671, 738
MaeIII GTNAC 2 cut(s) 122, 197
MalI GATC 4 cut(s) 218, 401, 414, 699
MboI GATC 4 cut(s) 216, 399, 412, 697
MboII GAAGA 8 cut(s) 23, 26, 70, 152, 175, 386, 479, 485
MflI RGATCY 2 cut(s) 399, 412
MhlI GDGCHC 3 cut(s) 180, 557, 710
MluCI AATT 7 cut(s) 188, 251, 261, 586, 618, 646, 653
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 7 cut(s) 285, 307, 411, 469, 535, 634, 654
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 3 cut(s) 264, 645, 744
MspA1I CMGCKG 3 cut(s) 96, 248, 532
MspR9I CCNGG 2 cut(s) 233, 494
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 2 cut(s) 233, 494
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeI CATATG 1 cut(s) 510
NdeII GATC 4 cut(s) 216, 399, 412, 697
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 3 cut(s) 465, 572, 686
NlaIV GGNNCC 2 cut(s) 177, 554
NmuCI GTSAC 1 cut(s) 197
NsbI TGCGCA 1 cut(s) 536
NspV TTCGAA 1 cut(s) 299
PceI AGGCCT 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 55
PfoI TCCNGGA 1 cut(s) 231
PkrI GCNGC 7 cut(s) 22, 25, 28, 531, 534, 608, 775
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 231, 492
PspGI CCWGG 2 cut(s) 231, 492
PspN4I GGNNCC 2 cut(s) 177, 554
PspPI GGNCC 1 cut(s) 490
PstI CTGCAG 1 cut(s) 608
PsuI RGATCY 2 cut(s) 399, 412
PvuII CAGCTG 2 cut(s) 96, 532
RsaI GTAC 2 cut(s) 576, 761
RsaNI GTAC 2 cut(s) 575, 760
SaqAI TTAA 3 cut(s) 264, 645, 744
SatI GCNGC 7 cut(s) 21, 24, 27, 530, 533, 607, 774
Sau3AI GATC 4 cut(s) 216, 399, 412, 697
Sau96I GGNCC 1 cut(s) 490
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 233, 494
SduI GDGCHC 3 cut(s) 180, 557, 710
SetI ASST 6 cut(s) 31, 98, 367, 534, 627, 645
SfaNI GCATC 2 cut(s) 369, 738
SfcI CTRYAG 1 cut(s) 604
SfuI TTCGAA 1 cut(s) 299
Sse9I AATT 7 cut(s) 188, 251, 261, 586, 618, 646, 653
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 3 cut(s) 24, 246, 497
SspMI CTAG 2 cut(s) 671, 738
StuI AGGCCT 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 231, 492
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 122
TaqI TCGA 2 cut(s) 299, 732
TasI AATT 7 cut(s) 188, 251, 261, 586, 618, 646, 653
TatI WGTACW 2 cut(s) 574, 759
TauI GCSGC 1 cut(s) 26
Tru1I TTAA 3 cut(s) 264, 645, 744
Tru9I TTAA 3 cut(s) 264, 645, 744
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 6 cut(s) 20, 26, 529, 532, 606, 773
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 3 cut(s) 354, 486, 671
TspGWI ACGGA 1 cut(s) 440
VneI GTGCAC 1 cut(s) 706
XapI RAATTY 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 2 cut(s) 671, 738
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.