pycom01g12870

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020382
Physical Location & Seq
Forward (+)
13507090 .. 13512479
5390 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1698 bp
ATGTCAACTTCCGAGCCCTCTGATAGTGTGATGGCAATGCTCATCGACGGAGAGGACGATCAGGAAGTACACGAACTGCAAACCGACGCTCGGACTCCGATTCCCAAGCAACGCCGAGAAATCTCGCCTTCGCTGTCGTCGAATTACGGTGGCGACGGCGACGAAGGCAGAAGAAAAAAGGTTTTGGCTTCGAACGATACAGAGGGTTTGTTTTGTCCTATTTGTATGGAGGCGTGGGAGTCACAAGGCGATCATCAAGTCAGTTGTCTTCCTTGTGGTCATGTATATGGCTTGTCTTGCATTAGAAGATGGATTCAACAACATGGTGGAATTGTTGCAAAGTGTCCACAATGCAATTCAAAGTACAACTTAAAGGACATAATAAAGCTGTTTGGATCACCAATAGTTGTTCTTGATGAAAGTCTACTTAAGAAAGTAAGATCATACGAGGCTGAAGTCGCCTCTCTCAAGACTGAAAGAGCTTCTTTACTTGCCATACAAGATGATTTATTCCGTGTACAAGAAAATCCATTCAAGGAATTAACCAACCGGGGGGAGAAACTTAGCTGCGGAGGTGATGCAGCATCAGGAGAGAAAGGGATGGAGCAGAGACAGGGCAGACAATGGGATCATCACTCTGCCCATAATTTTCTCAAAGAAGGAATTCTTCACTGTAAATTTCATTTGGAGTGTGAGCTGGCAGTAGAAGGGGCACGGCTTTTTGATATGGATGCTTCTTCTCAAACTTTGGTCCTTGCTCGAAGGATATCTGGATTAGGTGGCACACACATGCTGAAGAAAGTCAACTTGATGTCTCCTCAACAAAGTGAAGATATACAGCTTCCACCTGGTACAAAGCCCATTAAGGATCTACACGTATCCCCGTGTGGTAAATTCACCCTTTTAGCTTCACTGGGGAAGAAACTATCATTTCTTAGCTTGGGGAGCAACAGTTTAGTCATCACTTATGACTTACCGGGCCCAGCATGGTCGTGCTCATGGGATCTTAACAAGCCACATTGTATATATACTGGTTTGCAGGATGGTTTGCTTTTAAAGTTTGATATGCGTTTTAGCAAGCTTCCTCTGCTATCTTTGGCTGGGCTGACCTCCCGGCCAATTCATACTATACACTCCCTTGTGCAAAACCGTGCTTTCAGTCACACTTCTCATAAATTGCTCACTGCTTCTTCCAGCGGCCCTTGCGTGTGGAACATGGGTAGTGCTAGTCCTGCTGGAGAAAGGCCATTTCTCATCCCCGGATTAGAGGATCAAGGTGTTTGTACATCAGTAGCTTATAGTCCCTCAACTGATAACATAGTTGCTTCATATCATCCGAAAACCGATACATCCGCGAGACCTTCTGGTTCACAAGTTCTTATCAAGAGGGTAGATGATTGCTTTTATCATAAGATCGGATCTGTGCCAGTTCATTTGAATGACTTTCAGCTGAGAAAGTCTGCCATTGTAGATATACCAGACTGCTACCCATTGTTTGCGTATGGTGAGGAACTGACCCGTGGATTGAGGCTGCGGGAATTGCCAAGTCTAAAAGTGAGCCAGAATCTTGAACCTCATCAACATTCCATCCTTGACGTGAAGTATGTACGTTCTCAGGACAAAGGTTTGCTTGGTTGCTTGAGCGAGGACAAGTTGCAACTTTTCTCCGCGGCTACAAAAGGTGTGTTTATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

566

Amino Acids

62.32

Weight (kDa)

6.87

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 72 - 119 2.8e-08 Ring finger domain
zf-RING_UBOX PF13445 72 - 116 6.9e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 236 - 555 3.8e-26 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020382)

Species Orthologous Gene IDs
malus_domestica MD01G1102300.v1.1
prunus_persica Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1
pyrus_communis pycom01g12870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 424
AccII CGCG 2 cut(s) 1355, 1670
AciI CCGC 6 cut(s) 570, 1197, 1353, 1534, 1668, 1670
AclWI GGATC 6 cut(s) 403, 636, 876, 1011, 1278, 1426
AcoI YGGCCR 1 cut(s) 1115
AcsI RAATTY 3 cut(s) 663, 677, 893
AcuI CTGAAG 2 cut(s) 474, 815
AfaI GTAC 6 cut(s) 69, 365, 519, 853, 1285, 1608
AfiI CCNNNNNNNGG 2 cut(s) 90, 552
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 5 cut(s) 317, 360, 535, 1438, 1571
AjiI CACGTC 1 cut(s) 1597
AjnI CCWGG 1 cut(s) 847
Alw21I GWGCWC 1 cut(s) 998
Alw26I GTCTC 3 cut(s) 604, 819, 1351
AlwI GGATC 6 cut(s) 403, 636, 876, 1011, 1278, 1426
AoxI GGCC 4 cut(s) 979, 1115, 1198, 1244
ApaI GGGCCC 1 cut(s) 983
ApeKI GCWGC 3 cut(s) 567, 581, 1531
ApoI RAATTY 3 cut(s) 663, 677, 893
Asp700I GAANNNNTTC 1 cut(s) 663
AspS9I GGNCC 4 cut(s) 751, 979, 980, 1199
AsuC2I CCSGG 4 cut(s) 551, 978, 1114, 1260
AsuHPI GGTGA 4 cut(s) 390, 587, 889, 1517
AsuII TTCGAA 1 cut(s) 191
AvaII GGWCC 1 cut(s) 751
BaeGI GKGCMC 2 cut(s) 715, 983
BanII GRGCYC 2 cut(s) 18, 983
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 998
BbvI GCAGC 3 cut(s) 554, 593, 1518
BccI CCATC 5 cut(s) 25, 303, 595, 1037, 1595
BceAI ACGGC 2 cut(s) 172, 731
BciT130I CCWGG 1 cut(s) 849
BciVI GTATCC 1 cut(s) 889
BcnI CCSGG 4 cut(s) 551, 978, 1114, 1260
BcoDI GTCTC 3 cut(s) 604, 819, 1351
BfaI CTAG 1 cut(s) 1227
BfrI CTTAAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 5 cut(s) 568, 582, 1198, 1532, 1671
BlsI GCNGC 5 cut(s) 569, 583, 1199, 1533, 1672
Bme1390I CCNGG 5 cut(s) 551, 849, 978, 1114, 1260
Bme18I GGWCC 1 cut(s) 751
BmgBI CACGTC 1 cut(s) 1597
BmgT120I GGNCC 4 cut(s) 751, 979, 980, 1199
BmiI GGNNCC 1 cut(s) 981
BmrFI CCNGG 5 cut(s) 551, 849, 978, 1114, 1260
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 3 cut(s) 568, 593, 721
BmuI ACTGGG 1 cut(s) 923
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 452, 1660
BpuMI CCSGG 4 cut(s) 551, 978, 1114, 1260
BsaAI YACGTR 1 cut(s) 877
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 4 cut(s) 550, 1258, 1519, 1668
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 552
Bse1I ACTGG 3 cut(s) 918, 1036, 1427
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 849
BseDI CCNNGG 4 cut(s) 550, 1258, 1519, 1668
BseGI GGATG 7 cut(s) 606, 736, 1048, 1254, 1333, 1349, 1587
BseLI CCNNNNNNNGG 2 cut(s) 90, 552
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 1442, 1628
BseNI ACTGG 3 cut(s) 918, 1036, 1427
BseRI GAGGAG 1 cut(s) 807
BseSI GKGCMC 2 cut(s) 715, 983
BseXI GCAGC 3 cut(s) 554, 593, 1518
BseYI CCCAGC 2 cut(s) 982, 1100
Bsh1236I CGCG 2 cut(s) 1355, 1670
BshFI GGCC 4 cut(s) 981, 1117, 1200, 1246
BsiHKAI GWGCWC 1 cut(s) 998
BsiSI CCGG 4 cut(s) 550, 977, 1114, 1260
BslFI GGGAC 1 cut(s) 1287
BslI CCNNNNNNNGG 2 cut(s) 90, 552
BsmAI GTCTC 3 cut(s) 604, 819, 1351
BsmFI GGGAC 1 cut(s) 1287
BsnI GGCC 4 cut(s) 981, 1117, 1200, 1246
Bso31I GGTCTC 1 cut(s) 1351
Bsp119I TTCGAA 1 cut(s) 191
Bsp120I GGGCCC 1 cut(s) 979
Bsp1286I GDGCHC 4 cut(s) 18, 715, 983, 998
Bsp1407I TGTACA 2 cut(s) 517, 1283
BspACI CCGC 6 cut(s) 570, 1197, 1353, 1534, 1668, 1670
BspANI GGCC 4 cut(s) 981, 1117, 1200, 1246
BspCNI CTCAG 2 cut(s) 1443, 1627
BspFNI CGCG 2 cut(s) 1355, 1670
BspLI GGNNCC 1 cut(s) 981
BspPI GGATC 6 cut(s) 403, 636, 876, 1011, 1278, 1426
BspT104I TTCGAA 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 1351
BsrDI GCAATG 1 cut(s) 42
BsrGI TGTACA 2 cut(s) 517, 1283
BsrI ACTGG 3 cut(s) 918, 1036, 1427
BssECI CCNNGG 4 cut(s) 550, 1258, 1519, 1668
Bst2UI CCWGG 1 cut(s) 849
Bst4CI ACNGT 4 cut(s) 149, 674, 953, 1151
BstAFI CTTAAG 1 cut(s) 428
BstAUI TGTACA 2 cut(s) 517, 1283
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 699, 1079
BstDEI CTNAG 4 cut(s) 563, 935, 1451, 1614
BstDSI CCRYGG 2 cut(s) 1519, 1668
BstF5I GGATG 7 cut(s) 606, 736, 1048, 1254, 1333, 1349, 1587
BstFNI CGCG 2 cut(s) 1355, 1670
BstMAI GTCTC 3 cut(s) 604, 819, 1351
BstMWI GCNNNNNNNGC 8 cut(s) 165, 297, 458, 945, 1087, 1203, 1232, 1540
BstNI CCWGG 1 cut(s) 849
BstNSI RCATGY 1 cut(s) 793
BstSCI CCNGG 5 cut(s) 549, 847, 976, 1112, 1258
BstSLI GKGCMC 2 cut(s) 715, 983
BstUI CGCG 2 cut(s) 1355, 1670
BstV1I GCAGC 3 cut(s) 554, 593, 1518
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 3 cut(s) 868, 1003, 1418
BstYI RGATCY 3 cut(s) 868, 1003, 1418
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 4 cut(s) 981, 1117, 1200, 1246
BtgI CCRYGG 2 cut(s) 1519, 1668
BtrI CACGTC 1 cut(s) 1597
BtsCI GGATG 7 cut(s) 606, 736, 1048, 1254, 1333, 1349, 1587
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 3 cut(s) 670, 911, 1182
Cac8I GCNNGC 2 cut(s) 699, 1079
Cfr13I GGNCC 4 cut(s) 751, 979, 980, 1199
Cfr42I CCGCGG 1 cut(s) 1671
CseI GACGC 1 cut(s) 95
CsiI ACCWGGT 1 cut(s) 847
Csp6I GTAC 6 cut(s) 68, 364, 518, 852, 1284, 1607
CviAII CATG 6 cut(s) 281, 323, 790, 987, 999, 1216
CviQI GTAC 6 cut(s) 68, 364, 518, 852, 1284, 1607
DdeI CTNAG 4 cut(s) 563, 935, 1451, 1614
DraI TTTAAA 1 cut(s) 1056
EaeI YGGCCR 1 cut(s) 1115
Eco24I GRGCYC 2 cut(s) 18, 983
Eco31I GGTCTC 1 cut(s) 1351
Eco32I GATATC 1 cut(s) 768
Eco47I GGWCC 1 cut(s) 751
Eco57I CTGAAG 2 cut(s) 474, 815
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 1 cut(s) 847
EcoRV GATATC 1 cut(s) 768
EcoT38I GRGCYC 2 cut(s) 18, 983
FaeI CATG 6 cut(s) 284, 326, 793, 990, 1002, 1219
FalI AAGNNNNNCTT 8 cut(s) 353, 385, 469, 501, 651, 683, 1614, 1646
FaqI GGGAC 1 cut(s) 1287
FatI CATG 6 cut(s) 280, 322, 789, 986, 998, 1215
FauI CCCGC 1 cut(s) 1527
FblI GTMKAC 1 cut(s) 424
Fnu4HI GCNGC 5 cut(s) 568, 582, 1198, 1532, 1671
FokI GGATG 7 cut(s) 613, 743, 1055, 1241, 1320, 1336, 1574
FriOI GRGCYC 2 cut(s) 18, 983
Fsp4HI GCNGC 5 cut(s) 568, 582, 1198, 1532, 1671
FspBI CTAG 1 cut(s) 1227
GluI GCNGC 5 cut(s) 568, 582, 1198, 1532, 1671
GsaI CCCAGC 2 cut(s) 986, 1104
GsuI CTGGAG 1 cut(s) 1257
HaeIII GGCC 4 cut(s) 981, 1117, 1200, 1246
HapII CCGG 4 cut(s) 550, 977, 1114, 1260
HgaI GACGC 1 cut(s) 95
Hin1II CATG 6 cut(s) 284, 326, 793, 990, 1002, 1219
HincII GTYRAC 2 cut(s) 6, 805
HindII GTYRAC 2 cut(s) 6, 805
HindIII AAGCTT 1 cut(s) 1079
HinfI GANTC 5 cut(s) 94, 100, 239, 313, 1564
HpaII CCGG 4 cut(s) 550, 977, 1114, 1260
HphI GGTGA 4 cut(s) 390, 587, 889, 1517
Hpy166II GTNNAC 7 cut(s) 6, 70, 347, 425, 518, 805, 1370
Hpy188I TCNGA 6 cut(s) 13, 22, 93, 99, 1338, 1418
Hpy188III TCNNGA 8 cut(s) 62, 413, 469, 588, 771, 1384, 1568, 1616
Hpy8I GTNNAC 7 cut(s) 6, 70, 347, 425, 518, 805, 1370
Hpy99I CGWCG 5 cut(s) 50, 89, 142, 158, 164
HpyAV CCTTC 6 cut(s) 138, 158, 653, 701, 756, 1371
HpyCH4III ACNGT 4 cut(s) 149, 674, 953, 1151
HpyCH4IV ACGT 3 cut(s) 876, 1596, 1609
HpyCH4V TGCA 8 cut(s) 79, 300, 338, 354, 581, 1039, 1144, 1657
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 297, 458, 945, 1087, 1203, 1232, 1540
HpyF3I CTNAG 4 cut(s) 563, 935, 1451, 1614
HpySE526I ACGT 3 cut(s) 876, 1596, 1609
Hsp92II CATG 6 cut(s) 284, 326, 793, 990, 1002, 1219
KspI CCGCGG 1 cut(s) 1671
LmnI GCTCC 2 cut(s) 604, 945
Lsp1109I GCAGC 3 cut(s) 554, 593, 1518
LweI GCATC 3 cut(s) 568, 593, 721
MabI ACCWGGT 1 cut(s) 847
MaeI CTAG 1 cut(s) 1227
MaeII ACGT 3 cut(s) 876, 1596, 1609
MaeIII GTNAC 2 cut(s) 240, 1160
MboII GAAGA 9 cut(s) 183, 260, 318, 659, 729, 808, 842, 931, 1182
MflI RGATCY 3 cut(s) 868, 1003, 1418
MhlI GDGCHC 4 cut(s) 18, 715, 983, 998
MlyI GAGTC 2 cut(s) 88, 248
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 6 cut(s) 371, 429, 542, 864, 1008, 1055
MslI CAYNNNNRTG 4 cut(s) 285, 788, 991, 1437
MspA1I CMGCKG 3 cut(s) 1197, 1450, 1670
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 4 cut(s) 550, 977, 1114, 1260
MspR9I CCNGG 5 cut(s) 551, 849, 978, 1114, 1260
MvaI CCWGG 1 cut(s) 849
MvnI CGCG 2 cut(s) 1355, 1670
MwoI GCNNNNNNNGC 8 cut(s) 165, 297, 458, 945, 1087, 1203, 1232, 1540
NciI CCSGG 4 cut(s) 551, 978, 1114, 1260
NlaIII CATG 6 cut(s) 284, 326, 793, 990, 1002, 1219
NlaIV GGNNCC 1 cut(s) 981
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 2 cut(s) 240, 1160
NspI RCATGY 1 cut(s) 793
NspV TTCGAA 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 137
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 100, 313, 1564
PkrI GCNGC 5 cut(s) 569, 583, 1199, 1533, 1672
PleI GAGTC 2 cut(s) 88, 247
PpsI GAGTC 2 cut(s) 88, 247
Ppu21I YACGTR 1 cut(s) 877
Psp6I CCWGG 1 cut(s) 847
PspFI CCCAGC 2 cut(s) 982, 1100
PspGI CCWGG 1 cut(s) 847
PspN4I GGNNCC 1 cut(s) 981
PspOMI GGGCCC 1 cut(s) 979
PspPI GGNCC 4 cut(s) 751, 979, 980, 1199
PsuI RGATCY 3 cut(s) 868, 1003, 1418
PvuII CAGCTG 1 cut(s) 1450
RsaI GTAC 6 cut(s) 69, 365, 519, 853, 1285, 1608
RsaNI GTAC 6 cut(s) 68, 364, 518, 852, 1284, 1607
RseI CAYNNNNRTG 4 cut(s) 285, 788, 991, 1437
SacII CCGCGG 1 cut(s) 1671
SaqAI TTAA 6 cut(s) 371, 429, 542, 864, 1008, 1055
SatI GCNGC 5 cut(s) 568, 582, 1198, 1532, 1671
Sau96I GGNCC 4 cut(s) 751, 979, 980, 1199
SchI GAGTC 2 cut(s) 88, 248
ScrFI CCNGG 5 cut(s) 551, 849, 978, 1114, 1260
SduI GDGCHC 4 cut(s) 18, 715, 983, 998
SexAI ACCWGGT 1 cut(s) 847
SfaNI GCATC 3 cut(s) 568, 593, 721
Sfr303I CCGCGG 1 cut(s) 1671
SfuI TTCGAA 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 1671
SinI GGWCC 1 cut(s) 751
SmiMI CAYNNNNRTG 4 cut(s) 285, 788, 991, 1437
SmlI CTYRAG 3 cut(s) 428, 467, 1639
SmoI CTYRAG 3 cut(s) 428, 467, 1639
SsiI CCGC 6 cut(s) 570, 1197, 1353, 1534, 1668, 1670
SspMI CTAG 1 cut(s) 1227
StyD4I CCNGG 5 cut(s) 549, 847, 976, 1112, 1258
TaaI ACNGT 4 cut(s) 149, 674, 953, 1151
TaiI ACGT 3 cut(s) 879, 1599, 1612
TaqI TCGA 4 cut(s) 45, 140, 191, 760
TatI WGTACW 4 cut(s) 67, 363, 517, 1283
TauI GCSGC 2 cut(s) 1200, 1673
TfiI GAWTC 3 cut(s) 100, 313, 1564
Tru1I TTAA 6 cut(s) 371, 429, 542, 864, 1008, 1055
Tru9I TTAA 6 cut(s) 371, 429, 542, 864, 1008, 1055
TscAI CASTG 3 cut(s) 677, 918, 1189
TseFI GTSAC 2 cut(s) 240, 1160
TseI GCWGC 3 cut(s) 567, 581, 1531
Tsp45I GTSAC 2 cut(s) 240, 1160
TspDTI ATGAA 5 cut(s) 432, 671, 1112, 1317, 1421
TspGWI ACGGA 2 cut(s) 63, 503
TspRI CASTG 3 cut(s) 677, 918, 1189
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 751
XapI RAATTY 3 cut(s) 663, 677, 893
XceI RCATGY 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 1 cut(s) 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.