pycom01g18550

Metacaspase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0028951
Physical Location & Seq
Forward (+)
17499328 .. 17500899
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18550.1

Sequence Viewer

Length: 1098 bp
ATGTCGATGCCTGTACAATGCTCGAACTGCCACAAAAAGGTGCAGCTACTACGGCCAGGCGAAGCCCCCGTCTACCGCCCCATATGCCGGGTTGCCACCTATACAGCCCCGTATTCGTATTCCCACCACTGCCCGCCTCATCAACCGCCCCTCCTCACCCTGCCTCCACCCCCGTACAGCCAAATACCACCAGGGACACCTATGTTGGCTCGAGGATGCAAACGAGCTGTGATATGCGCTGTGTCGTATAAGGACTCCAAGCATGAGCTTAAGGGCTGGATCAATGATGCAAAATGTATGAAGCACTTGCTTGTCAATCGGTTCCACTTCCCGGATTCCTCCATTCTCATGCTCACCGAAGAAGAAACTGACCCAAATCCGCATCCTACCAAACAAAACATGATAAGGGCTCTGCATTGGCTTGTGCAAGGTTGGCAACCAGGAGACTCCTTGGTGTTCCATTATTCCGGTCATGGAAAAAGAAAGAGGAACTACACCGGGGATGAGGTGGATGGATATGACGAAAACTTGTGTCCCGTGGATTTCAAGAGTCGGGGACTGATTTCAGACGATGAGATCAATGCAATGATTGTAAGACCTCTTCCTCGTGGGGTTAAGCCTCACACCTTTATGGATGCTTGCCATAGCGGTACTGCTCTAGATTTAGCATTCCTTTGCAGGATCGACAGAAGTGGGAGATACGTATGGGAGGATCACCGCCCTCCATCGGGTGTTTGGAAAGGAACAAATGGCGGGAAGGTCATATGCCTCAGTGGCTGTGATGATCATCAGACCTCTGCTGATGCTTCAAATCTGTCGACTAGTACTCCAACTGGTGTCATGACTTTTTCTTTCACCCAAGCCATCGAGCATGGACATGGAACCACATACGGCAGCATCATGAGGGCGATAAGATCTACCATTCTCAACACGGAGAATGAAGGCCGTGATACCTCAACGTCATCTCCGAGTCTTGCTGGCAGGTTGACACAGCAAGTAAAATGCTTAAGCGGAAGAAGAAATTCTCCCTGTGATTTTCGTCATCTGTTTTCGTTCTCCTGTTCTGTATACGGAAATGTGAAGGTCCAAATTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14 zf-LSD1

Protein Analysis

366

Amino Acids

40.61

Weight (kDa)

8.76

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 76 - 312 1.5e-31 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028951)

Species Orthologous Gene IDs
prunus_persica Prupe.2G269300_v2.0.a1
pyrus_communis pycom01g18550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 972
AccI GTMKAC 3 cut(s) 72, 818, 1068
AciI CCGC 8 cut(s) 76, 134, 146, 380, 648, 718, 753, 1011
AclWI GGATC 3 cut(s) 287, 689, 720
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 2 cut(s) 1021, 1089
AfaI GTAC 4 cut(s) 15, 176, 652, 826
AfiI CCNNNNNNNGG 5 cut(s) 37, 87, 331, 727, 728
AflII CTTAAG 2 cut(s) 269, 1006
AgsI TTSAA 2 cut(s) 547, 810
AhlI ACTAGT 1 cut(s) 821
AjnI CCWGG 3 cut(s) 55, 190, 439
AluBI AGCT 3 cut(s) 46, 227, 268
AluI AGCT 3 cut(s) 46, 227, 268
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 3 cut(s) 287, 689, 720
AlwNI CAGNNNCTG 1 cut(s) 777
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 2 cut(s) 53, 943
ApeKI GCWGC 2 cut(s) 43, 894
ApoI RAATTY 2 cut(s) 1021, 1089
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
Asp700I GAANNNNTTC 1 cut(s) 1021
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 1084
AsuC2I CCSGG 3 cut(s) 89, 332, 499
AsuHPI GGTGA 4 cut(s) 148, 346, 707, 847
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 412
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BauI CACGAG 1 cut(s) 606
BbvI GCAGC 2 cut(s) 55, 906
BccI CCATC 3 cut(s) 506, 733, 872
BceAI ACGGC 3 cut(s) 68, 907, 930
BciT130I CCWGG 3 cut(s) 57, 192, 441
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 3 cut(s) 89, 332, 499
BcoDI GTCTC 1 cut(s) 438
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 2 cut(s) 659, 822
BfrI CTTAAG 2 cut(s) 269, 1006
BfuAI ACCTGC 1 cut(s) 972
BglI GCCNNNNNGGC 1 cut(s) 774
BglII AGATCT 1 cut(s) 914
BisI GCNGC 2 cut(s) 44, 895
BlsI GCNGC 2 cut(s) 45, 896
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 6 cut(s) 57, 89, 192, 332, 441, 499
Bme18I GGWCC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 1084
BmiI GGNNCC 2 cut(s) 323, 883
BmrFI CCNGG 6 cut(s) 57, 89, 192, 332, 441, 499
BmsI GCATC 6 cut(s) 206, 277, 391, 625, 793, 906
BpuMI CCSGG 3 cut(s) 89, 332, 499
BsaAI YACGTR 1 cut(s) 703
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 4 cut(s) 191, 450, 498, 537
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 8 cut(s) 135, 148, 165, 178, 811, 841, 949, 979
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 87, 331, 727, 728
Bse1I ACTGG 1 cut(s) 838
Bse3DI GCAATG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 3 cut(s) 57, 192, 441
BseDI CCNNGG 4 cut(s) 191, 450, 498, 537
BseGI GGATG 5 cut(s) 221, 382, 508, 517, 640
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 5 cut(s) 37, 87, 331, 727, 728
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 1 cut(s) 784
BseNI ACTGG 1 cut(s) 838
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 55, 906
BsgI GTGCAG 1 cut(s) 62
BshFI GGCC 2 cut(s) 55, 945
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 4 cut(s) 88, 332, 468, 498
BslFI GGGAC 3 cut(s) 208, 519, 570
BslI CCNNNNNNNGG 5 cut(s) 37, 87, 331, 727, 728
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 3 cut(s) 208, 519, 570
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 2 cut(s) 55, 945
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 13
Bsp143I GATC 6 cut(s) 279, 576, 681, 712, 784, 914
BspACI CCGC 8 cut(s) 76, 134, 146, 380, 648, 718, 753, 1011
BspANI GGCC 2 cut(s) 55, 945
BspCNI CTCAG 1 cut(s) 783
BspHI TCATGA 2 cut(s) 840, 900
BspLI GGNNCC 2 cut(s) 323, 883
BspMI ACCTGC 1 cut(s) 972
BspPI GGATC 3 cut(s) 287, 689, 720
BspTI CTTAAG 2 cut(s) 269, 1006
BsrDI GCAATG 1 cut(s) 591
BsrGI TGTACA 1 cut(s) 13
BsrI ACTGG 1 cut(s) 838
BssECI CCNNGG 4 cut(s) 191, 450, 498, 537
BssMI GATC 6 cut(s) 279, 576, 681, 712, 784, 914
BssNAI GTATAC 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 450
Bst1107I GTATAC 1 cut(s) 1069
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 57, 192, 441
Bst6I CTCTTC 1 cut(s) 606
BstAFI CTTAAG 2 cut(s) 269, 1006
BstAUI TGTACA 1 cut(s) 13
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 3 cut(s) 134, 640, 979
BstDEI CTNAG 1 cut(s) 770
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 5 cut(s) 221, 382, 508, 517, 640
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 6 cut(s) 282, 579, 684, 715, 787, 917
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 6 cut(s) 279, 576, 681, 712, 784, 914
BstMWI GCNNNNNNNGC 5 cut(s) 27, 52, 84, 433, 774
BstNI CCWGG 3 cut(s) 57, 192, 441
BstSCI CCNGG 6 cut(s) 55, 87, 190, 330, 439, 497
BstSNI TACGTA 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 55, 906
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BstZ17I GTATAC 1 cut(s) 1069
BsuRI GGCC 2 cut(s) 55, 945
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 5 cut(s) 221, 382, 508, 517, 640
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 127, 778
BveI ACCTGC 1 cut(s) 972
Cac8I GCNNGC 3 cut(s) 134, 640, 979
CaiI CAGNNNCTG 1 cut(s) 777
CciI TCATGA 2 cut(s) 840, 900
CfoI GCGC 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 1084
Csp6I GTAC 4 cut(s) 14, 175, 651, 825
CviAII CATG 8 cut(s) 263, 349, 400, 473, 841, 872, 878, 901
CviQI GTAC 4 cut(s) 14, 175, 651, 825
DdeI CTNAG 1 cut(s) 770
DpnI GATC 6 cut(s) 281, 578, 683, 714, 786, 916
DpnII GATC 6 cut(s) 279, 576, 681, 712, 784, 914
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco105I TACGTA 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 1084
Eco88I CYCGRG 1 cut(s) 210
EcoRII CCWGG 3 cut(s) 55, 190, 439
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 8 cut(s) 266, 352, 403, 476, 844, 875, 881, 904
FaqI GGGAC 3 cut(s) 208, 519, 570
FatI CATG 8 cut(s) 262, 348, 399, 472, 840, 871, 877, 900
FauI CCCGC 2 cut(s) 141, 746
FauNDI CATATG 2 cut(s) 83, 764
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 3 cut(s) 72, 818, 1068
Fnu4HI GCNGC 2 cut(s) 44, 895
FokI GGATG 5 cut(s) 228, 369, 515, 524, 647
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 2 cut(s) 44, 895
FspBI CTAG 2 cut(s) 659, 822
GlaI GCGC 1 cut(s) 238
GluI GCNGC 2 cut(s) 44, 895
HaeIII GGCC 2 cut(s) 55, 945
HapII CCGG 4 cut(s) 88, 332, 468, 498
HhaI GCGC 1 cut(s) 239
Hin1II CATG 8 cut(s) 266, 352, 403, 476, 844, 875, 881, 904
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 2 cut(s) 819, 987
HindII GTYRAC 2 cut(s) 819, 987
HinfI GANTC 5 cut(s) 254, 335, 446, 550, 970
HpaII CCGG 4 cut(s) 88, 332, 468, 498
HphI GGTGA 4 cut(s) 148, 346, 707, 847
Hpy166II GTNNAC 4 cut(s) 73, 819, 987, 1069
Hpy188I TCNGA 3 cut(s) 568, 792, 969
Hpy188III TCNNGA 4 cut(s) 547, 659, 841, 901
Hpy8I GTNNAC 4 cut(s) 73, 819, 987, 1069
HpyAV CCTTC 3 cut(s) 751, 935, 1075
HpyCH4IV ACGT 2 cut(s) 702, 959
HpyCH4V TGCA 7 cut(s) 43, 219, 290, 415, 427, 584, 678
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 52, 84, 433, 774
HpyF3I CTNAG 1 cut(s) 770
HpySE526I ACGT 2 cut(s) 702, 959
Hsp92II CATG 8 cut(s) 266, 352, 403, 476, 844, 875, 881, 904
HspAI GCGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 6 cut(s) 279, 576, 681, 712, 784, 914
Lsp1109I GCAGC 2 cut(s) 55, 906
LweI GCATC 6 cut(s) 206, 277, 391, 625, 793, 906
MaeI CTAG 2 cut(s) 659, 822
MaeII ACGT 2 cut(s) 702, 959
MalI GATC 6 cut(s) 281, 578, 683, 714, 786, 916
MboI GATC 6 cut(s) 279, 576, 681, 712, 784, 914
MboII GAAGA 5 cut(s) 371, 374, 593, 1026, 1029
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 2 cut(s) 1021, 1089
MlyI GAGTC 4 cut(s) 248, 440, 559, 979
MmeI TCCRAC 1 cut(s) 854
MroXI GAANNNNTTC 1 cut(s) 1021
MseI TTAA 3 cut(s) 270, 615, 1007
MslI CAYNNNNRTG 3 cut(s) 347, 629, 876
MspCI CTTAAG 2 cut(s) 269, 1006
MspI CCGG 4 cut(s) 88, 332, 468, 498
MspR9I CCNGG 6 cut(s) 57, 89, 192, 332, 441, 499
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 3 cut(s) 57, 192, 441
MwoI GCNNNNNNNGC 5 cut(s) 27, 52, 84, 433, 774
NciI CCSGG 3 cut(s) 89, 332, 499
NdeI CATATG 2 cut(s) 83, 764
NdeII GATC 6 cut(s) 279, 576, 681, 712, 784, 914
NlaIII CATG 8 cut(s) 266, 352, 403, 476, 844, 875, 881, 904
NlaIV GGNNCC 2 cut(s) 323, 883
PaeR7I CTCGAG 1 cut(s) 210
PagI TCATGA 2 cut(s) 840, 900
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 1021
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 330
PkrI GCNGC 2 cut(s) 45, 896
PleI GAGTC 4 cut(s) 248, 440, 558, 978
PpsI GAGTC 4 cut(s) 248, 440, 558, 978
Ppu21I YACGTR 1 cut(s) 703
Psp6I CCWGG 3 cut(s) 55, 190, 439
PspGI CCWGG 3 cut(s) 55, 190, 439
PspN4I GGNNCC 2 cut(s) 323, 883
PspPI GGNCC 1 cut(s) 1084
PspXI VCTCGAGB 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 777
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 4 cut(s) 15, 176, 652, 826
RsaNI GTAC 4 cut(s) 14, 175, 651, 825
RseI CAYNNNNRTG 3 cut(s) 347, 629, 876
SalI GTCGAC 1 cut(s) 817
SaqAI TTAA 3 cut(s) 270, 615, 1007
SatI GCNGC 2 cut(s) 44, 895
Sau3AI GATC 6 cut(s) 279, 576, 681, 712, 784, 914
Sau96I GGNCC 1 cut(s) 1084
ScaI AGTACT 1 cut(s) 826
SchI GAGTC 4 cut(s) 248, 440, 559, 979
ScrFI CCNGG 6 cut(s) 57, 89, 192, 332, 441, 499
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 6 cut(s) 206, 277, 391, 625, 793, 906
Sfr274I CTCGAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 1084
SlaI CTCGAG 1 cut(s) 210
SmiMI CAYNNNNRTG 3 cut(s) 347, 629, 876
SmlI CTYRAG 3 cut(s) 210, 269, 1006
SmoI CTYRAG 3 cut(s) 210, 269, 1006
SnaBI TACGTA 1 cut(s) 703
SpeI ACTAGT 1 cut(s) 821
Sse9I AATT 2 cut(s) 1021, 1089
SsiI CCGC 8 cut(s) 76, 134, 146, 380, 648, 718, 753, 1011
SspMI CTAG 2 cut(s) 659, 822
StyD4I CCNGG 6 cut(s) 55, 87, 190, 330, 439, 497
StyI CCWWGG 1 cut(s) 450
TaiI ACGT 2 cut(s) 705, 962
TaqI TCGA 6 cut(s) 5, 23, 211, 684, 818, 867
TasI AATT 2 cut(s) 1021, 1089
TatI WGTACW 2 cut(s) 13, 824
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 3 cut(s) 270, 615, 1007
Tru9I TTAA 3 cut(s) 270, 615, 1007
TscAI CASTG 2 cut(s) 134, 778
TseI GCWGC 2 cut(s) 43, 894
TspDTI ATGAA 2 cut(s) 314, 954
TspGWI ACGGA 2 cut(s) 947, 1086
TspRI CASTG 2 cut(s) 134, 778
Vha464I CTTAAG 2 cut(s) 269, 1006
VpaK11BI GGWCC 1 cut(s) 1084
XapI RAATTY 2 cut(s) 1021, 1089
XbaI TCTAGA 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XhoI CTCGAG 1 cut(s) 210
XmiI GTMKAC 3 cut(s) 72, 818, 1068
XmnI GAANNNNTTC 1 cut(s) 1021
XspI CTAG 2 cut(s) 659, 822
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.