pycom01g19310

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019198
Physical Location & Seq
Forward (+)
18018074 .. 18018505
432 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGGCGTCCTCGCTTGCCCTCAGGCGACTTTTCCCCAACTTCAAGTTCCTTGCCGCGGATATCTCCCTCAGTCGCCCAAATCCAATCGCTGCAGCCACCCGCTCGGGCTTTGCTGCCTCCCGGAGGTACTTCTCTAACCCCGGAGGAGTTGATGGAGACGAGGATTTTTACGTGCGCGAGACGGATGAGGGTTATTACGTGCGAATGGAGATGGAGGGGCTTGATAAAGAGGACGTAAAGGTGTGGGTGAATGTGGAGGAGAACACTCTCAGCATCAAAGGCGAGGGCGGGTTCTGCAGCTTCCATCACCTGCCGGAGTTATGCAAGACTGACGGCGTGAAGGCGGAGCTGAAGAACGGCGTGCTCAAGGTGGCTGTTCCGAAGGTCAACGAACAGGACAGGGCTGAGTTGATTCATGTCAAGGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

144

Amino Acids

15.95

Weight (kDa)

5.46

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 102 - 142 7.7e-06 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019198)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05762
pyrus_communis pycom01g19310
rosa_laevigata RLG00000014742
rosa_multiflora Rmu_sc0007004.1_g000009
rosa_samantha Rh6AG083300 Rh6BG077900 Rh6CG072500 Rh6DG069700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 2 cut(s) 56, 177
AciI CCGC 5 cut(s) 54, 56, 100, 288, 344
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 2 cut(s) 55, 123
AgsI TTSAA 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 2 cut(s) 300, 349
AluI AGCT 2 cut(s) 300, 349
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 2 cut(s) 150, 173
Ama87I CYCGRG 1 cut(s) 103
ApeKI GCWGC 4 cut(s) 89, 92, 113, 297
AspLEI GCGC 1 cut(s) 177
AsuC2I CCSGG 2 cut(s) 121, 141
AsuHPI GGTGA 2 cut(s) 259, 299
AvaI CYCGRG 1 cut(s) 103
AxyI CCTNAGG 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 4 cut(s) 76, 100, 104, 309
BccI CCATC 3 cut(s) 146, 205, 312
BceAI ACGGC 2 cut(s) 349, 373
BcnI CCSGG 2 cut(s) 121, 141
BcoDI GTCTC 2 cut(s) 150, 173
BfmI CTRYAG 2 cut(s) 90, 295
BfuAI ACCTGC 1 cut(s) 318
BisI GCNGC 5 cut(s) 54, 90, 93, 114, 298
BlsI GCNGC 5 cut(s) 55, 91, 94, 115, 299
Bme1390I CCNGG 2 cut(s) 121, 141
BmeT110I CYCGRG 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 121, 141
BmsI GCATC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 121, 141
BsaAI YACGTR 2 cut(s) 172, 199
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 54, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 123
Bse21I CCTNAGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 54, 139
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 55, 123
BseMII CTCAG 4 cut(s) 34, 82, 283, 396
BseRI GAGGAG 2 cut(s) 159, 272
BseXI GCAGC 4 cut(s) 76, 100, 104, 309
Bsh1236I CGCG 2 cut(s) 56, 177
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 3 cut(s) 121, 141, 314
BslI CCNNNNNNNGG 2 cut(s) 55, 123
BsmAI GTCTC 2 cut(s) 150, 173
BsmBI CGTCTC 2 cut(s) 150, 173
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 366
BspACI CCGC 5 cut(s) 54, 56, 100, 288, 344
BspCNI CTCAG 4 cut(s) 33, 81, 282, 397
BspFNI CGCG 2 cut(s) 56, 177
BspMAI CTGCAG 2 cut(s) 94, 299
BspMI ACCTGC 1 cut(s) 318
BsrBI CCGCTC 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 54, 139
BssNI GRCGYC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 2 cut(s) 172, 199
BstC8I GCNNGC 2 cut(s) 15, 362
BstDEI CTNAG 4 cut(s) 20, 68, 269, 405
BstDSI CCRYGG 1 cut(s) 54
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 190
BstFNI CGCG 2 cut(s) 56, 177
BstHHI GCGC 1 cut(s) 177
BstMAI GTCTC 2 cut(s) 150, 173
BstMWI GCNNNNNNNGC 2 cut(s) 279, 294
BstSCI CCNGG 2 cut(s) 119, 139
BstSFI CTRYAG 2 cut(s) 90, 295
BstUI CGCG 2 cut(s) 56, 177
BstV1I GCAGC 4 cut(s) 76, 100, 104, 309
Bsu36I CCTNAGG 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 190
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 15, 362
CfoI GCGC 1 cut(s) 177
Cfr42I CCGCGG 1 cut(s) 57
Csp6I GTAC 1 cut(s) 127
CviAII CATG 1 cut(s) 416
CviJI RGCY 7 cut(s) 95, 108, 220, 300, 349, 374, 404
CviKI_1 RGCY 7 cut(s) 95, 108, 220, 300, 349, 374, 404
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 4 cut(s) 20, 68, 269, 405
EciI GGCGGA 1 cut(s) 359
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRV GATATC 1 cut(s) 61
Esp3I CGTCTC 2 cut(s) 150, 173
FaeI CATG 1 cut(s) 419
FaiI YATR 2 cut(s) 322, 417
FatI CATG 1 cut(s) 415
FauI CCCGC 2 cut(s) 107, 281
Fnu4HI GCNGC 5 cut(s) 54, 90, 93, 114, 298
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 5 cut(s) 54, 90, 93, 114, 298
GlaI GCGC 1 cut(s) 176
GluI GCNGC 5 cut(s) 54, 90, 93, 114, 298
HapII CCGG 3 cut(s) 121, 141, 314
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 1 cut(s) 412
HpaII CCGG 3 cut(s) 121, 141, 314
HphI GGTGA 2 cut(s) 259, 299
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 1 cut(s) 381
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 334, 376
HpyCH4IV ACGT 3 cut(s) 171, 198, 234
HpyCH4V TGCA 3 cut(s) 92, 297, 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 294
HpyF3I CTNAG 4 cut(s) 20, 68, 269, 405
HpySE526I ACGT 3 cut(s) 171, 198, 234
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 1 cut(s) 175
KspI CCGCGG 1 cut(s) 57
LmnI GCTCC 1 cut(s) 346
LpnPI CCDG 7 cut(s) 7, 134, 154, 323, 327, 380, 385
Lsp1109I GCAGC 4 cut(s) 76, 100, 104, 309
LweI GCATC 1 cut(s) 282
MaeII ACGT 3 cut(s) 171, 198, 234
MbiI CCGCTC 1 cut(s) 102
MboII GAAGA 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 3 cut(s) 121, 141, 314
MspR9I CCNGG 2 cut(s) 121, 141
MvnI CGCG 2 cut(s) 56, 177
MwoI GCNNNNNNNGC 2 cut(s) 279, 294
NciI CCSGG 2 cut(s) 121, 141
NlaIII CATG 1 cut(s) 419
PaqCI CACCTGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 412
PfoI TCCNGGA 1 cut(s) 119
PkrI GCNGC 5 cut(s) 55, 91, 94, 115, 299
Ppu21I YACGTR 2 cut(s) 172, 199
PstI CTGCAG 2 cut(s) 94, 299
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacII CCGCGG 1 cut(s) 57
SatI GCNGC 5 cut(s) 54, 90, 93, 114, 298
ScrFI CCNGG 2 cut(s) 121, 141
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 90, 295
Sfr303I CCGCGG 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SsiI CCGC 5 cut(s) 54, 56, 100, 288, 344
StyD4I CCNGG 2 cut(s) 119, 139
TaiI ACGT 3 cut(s) 174, 201, 237
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 412
TseI GCWGC 4 cut(s) 89, 92, 113, 297
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 1 cut(s) 197
XagI CCTNNNNNAGG 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.