pycom01g20730

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0022526
Physical Location & Seq
Forward (+)
19040231 .. 19042376
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20730.4

Sequence Viewer

Length: 1491 bp
ATGTTTTTTTCTGTAACTTACAATGTGCAAAGTGACATCTCTCTGCTGCAATGGCAATACAGACCACCATTACCTTGTCTCCATCAACGACTCAAAGGAACCCAAAACCCTAACTTCTCCATTGATATCCAAAACCATAACACCCCAACCAGAACAATCCATAGCTGCCATAGCTTTCCCAATGATACTCACCGGCTTATTGCTTTACTCAAGGTCAATGATCTCCATGCTCTTCCTCGGCGGCCTCGGCGAGCTCGCCTTAGCGGGCGGTTCCCTCGCCGTGGGCTTCGCCAACATCACCGGTTACTCTATCCTCTCCGGACTCGCCATGGGAATGGAACCTCTCTGCGGCCAAGCTTTTGGCGCAAAAAAAAACACTCTACTCGGCCTCTCTTTGCAGAGGACGGTCCTCCTCCTACTATTTACATCCTTACCCATTTCTCTTCTCTGGCTGAACGTGAAGAAAATCCTCCTCATCTGCGGCCAGGACGAAGCCATCCTTCTCGCTCAATCTTTTCTCCACCCTCTGAGAATTTACCTCAGAAGTCAATCCATCACTCTGCCTCTCACATTGTGCGCAACCCTCGCAATCATCCTCCACATTCCCATCAACTATTTACTCGTTTCCCACCTCGATTTGGGAATCAAAGCTTCCGTGATTGTATACATCATCGTCTCTGGGGTCCACAAGAAAACCTGGGGAGGGATTTCAATGGATTGTTTCAGAGAATGGAGGACTCTTCTAAATTTAGCGGTGCCCAGCTGCATTTCTGTGTGTTTAGAATGAAATCCGAGAGCCACCGTTGCTTCGATGGGCGTTCTAATTCAAACTACCGCTTTAATCTACATATTTCCTTCTTCCTTAAGCTTCAGTGTGTCCACGAGAGTCAGCAACGAAATCGGGGCGAACAACCCGAAAAAAGCGAAACTTGCAGCCATTGTAGGGCTCTGCTGCAGCTTTATGCTAGGCCTTTCAGCTCTGGTGTTTGCAACAACGGTGAGGAACATTTGGGCAAGCATGTTCACACAAGACAAAGACATTATAGCGCTAACATCGATGGTATTGCCGATAATCGGGCTCTGCGAGCTCGGAAACTGCCCTCAGACGACGGGGTGCGGAGTTCTGAGAGGCACTGCAAGGCCGAAAGTCGGAGCAAACATAAACTTGGGGTGCTTTTATCTTGTCGGAATGCCGGGGCTGTGGCTCGGCCTCTTGGCGGCGGAGGGCAGCTGCGCTCTGACCATGTTGGTGGTTTTGGGTCGCTGCTGAGGTGGCAGTTGGTGACAGCGAAGATGTTGAGGAGGACAAGCCAAGCAAAGCTGAAATCAAGGAAGATTGTTTATTGTTTCTTTTGGTACACTTCCACTGAAATTCAACTAATGCTGAATTTCATAGGAAGTAGCAAAATTGCTGGGTTAGGAACAAAAATCCCACCCTCTTCTGTATTTTGTAATCGGATACAAAAGAAATTCAAAGAAGATTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

497

Amino Acids

57.26

Weight (kDa)

10.83

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022526)

Species Orthologous Gene IDs
pyrus_communis pycom01g20730
rosa_samantha Rh2BG067300 Rh2CG068600 Rh2DG066900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 755
AccI GTMKAC 1 cut(s) 664
AccIII TCCGGA 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 350, 482
AcsI RAATTY 5 cut(s) 532, 746, 1371, 1387, 1469
AcuI CTGAAG 1 cut(s) 854
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 1 cut(s) 1358
AfeI AGCGCT 1 cut(s) 1048
AfiI CCNNNNNNNGG 8 cut(s) 281, 348, 638, 703, 943, 1074, 1149, 1217
AflII CTTAAG 1 cut(s) 863
AgeI ACCGGT 1 cut(s) 300
AgsI TTSAA 4 cut(s) 712, 828, 1376, 1474
AjnI CCWGG 2 cut(s) 484, 696
Alw21I GWGCWC 2 cut(s) 256, 1090
Alw26I GTCTC 2 cut(s) 83, 680
Aor13HI TCCGGA 1 cut(s) 318
Aor51HI AGCGCT 1 cut(s) 1048
AoxI GGCC 7 cut(s) 242, 350, 386, 482, 968, 1140, 1208
ApeKI GCWGC 9 cut(s) 46, 165, 763, 933, 952, 955, 1228, 1231, 1264
ApoI RAATTY 5 cut(s) 532, 746, 1371, 1387, 1469
AsiGI ACCGGT 1 cut(s) 300
AspLEI GCGC 4 cut(s) 366, 579, 1049, 1236
AspS9I GGNCC 2 cut(s) 407, 683
AsuC2I CCSGG 1 cut(s) 1195
AsuHPI GGTGA 4 cut(s) 182, 290, 1010, 1294
AvaII GGWCC 2 cut(s) 407, 683
BaeGI GKGCMC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 4 cut(s) 256, 949, 1081, 1090
BauI CACGAG 1 cut(s) 881
Bbv12I GWGCWC 2 cut(s) 256, 1090
BbvCI CCTCAGC 1 cut(s) 1268
BbvI GCAGC 9 cut(s) 33, 152, 750, 939, 945, 967, 1218, 1240, 1251
BccI CCATC 6 cut(s) 90, 504, 561, 615, 806, 1052
BceAI ACGGC 1 cut(s) 264
BcgI CGANNNNNNTGC 4 cut(s) 881, 915, 1046, 1080
BciT130I CCWGG 2 cut(s) 486, 698
BciVI GTATCC 1 cut(s) 1452
BcnI CCSGG 1 cut(s) 1195
BcoDI GTCTC 2 cut(s) 83, 680
BfaI CTAG 2 cut(s) 966, 1489
BfmI CTRYAG 1 cut(s) 953
BfoI RGCGCY 1 cut(s) 1050
BfrI CTTAAG 1 cut(s) 863
BfuI GTATCC 1 cut(s) 1452
Bme1390I CCNGG 3 cut(s) 486, 698, 1195
Bme18I GGWCC 2 cut(s) 407, 683
BmgT120I GGNCC 2 cut(s) 407, 683
BmiI GGNNCC 5 cut(s) 100, 272, 340, 684, 757
BmrFI CCNGG 3 cut(s) 486, 698, 1195
Bpu10I CCTNAGC 2 cut(s) 260, 1268
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 1195
Bsa29I ATCGAT 1 cut(s) 1056
BsaJI CCNNGG 6 cut(s) 236, 245, 280, 328, 697, 1194
BsaWI WCCGGW 2 cut(s) 300, 318
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 8 cut(s) 281, 348, 638, 703, 943, 1074, 1149, 1217
Bse118I RCCGGY 2 cut(s) 192, 300
Bse3DI GCAATG 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 2 cut(s) 486, 698
BseCI ATCGAT 1 cut(s) 1056
BseDI CCNNGG 6 cut(s) 236, 245, 280, 328, 697, 1194
BseGI GGATG 3 cut(s) 426, 496, 592
BseLI CCNNNNNNNGG 8 cut(s) 281, 348, 638, 703, 943, 1074, 1149, 1217
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 5 cut(s) 519, 554, 1116, 1116, 1259
BseRI GAGGAG 3 cut(s) 402, 462, 1315
BseSI GKGCMC 1 cut(s) 760
BseXI GCAGC 9 cut(s) 33, 152, 750, 939, 945, 967, 1218, 1240, 1251
BseYI CCCAGC 2 cut(s) 759, 1412
BshFI GGCC 7 cut(s) 244, 352, 388, 484, 970, 1142, 1210
BshNI GGYRCC 1 cut(s) 755
BshTI ACCGGT 1 cut(s) 300
BshVI ATCGAT 1 cut(s) 1056
BsiHKAI GWGCWC 2 cut(s) 256, 1090
BsiSI CCGG 4 cut(s) 193, 301, 319, 1194
BslI CCNNNNNNNGG 8 cut(s) 281, 348, 638, 703, 943, 1074, 1149, 1217
BsmAI GTCTC 2 cut(s) 83, 680
BsmBI CGTCTC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 1195
BsnI GGCC 7 cut(s) 244, 352, 388, 484, 970, 1142, 1210
Bsp1286I GDGCHC 5 cut(s) 256, 760, 949, 1081, 1090
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 1 cut(s) 220
Bsp19I CCATGG 1 cut(s) 328
BspANI GGCC 7 cut(s) 244, 352, 388, 484, 970, 1142, 1210
BspCNI CTCAG 5 cut(s) 520, 553, 1115, 1117, 1260
BspDI ATCGAT 1 cut(s) 1056
BspEI TCCGGA 1 cut(s) 318
BspLI GGNNCC 5 cut(s) 100, 272, 340, 684, 757
BspMAI CTGCAG 1 cut(s) 957
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 192, 300
BssAI RCCGGY 2 cut(s) 192, 300
BssECI CCNNGG 6 cut(s) 236, 245, 280, 328, 697, 1194
BssMI GATC 1 cut(s) 220
BssNAI GTATAC 1 cut(s) 665
BssSI CACGAG 1 cut(s) 881
BssT1I CCWWGG 1 cut(s) 328
Bst1107I GTATAC 1 cut(s) 665
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 2 cut(s) 486, 698
Bst4CI ACNGT 3 cut(s) 407, 803, 998
Bst6I CTCTTC 4 cut(s) 237, 448, 745, 1444
BstAFI CTTAAG 1 cut(s) 863
BstC8I GCNNGC 5 cut(s) 252, 256, 266, 1016, 1086
BstDEI CTNAG 6 cut(s) 260, 528, 540, 1102, 1125, 1268
BstDSI CCRYGG 2 cut(s) 280, 328
BstF5I GGATG 3 cut(s) 426, 496, 592
BstH2I RGCGCY 1 cut(s) 1050
BstHHI GCGC 4 cut(s) 366, 579, 1049, 1236
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 2 cut(s) 83, 680
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 9 cut(s) 52, 171, 247, 363, 585, 804, 930, 1085, 1273
BstNI CCWGG 2 cut(s) 486, 698
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 3 cut(s) 484, 696, 1193
BstSFI CTRYAG 1 cut(s) 953
BstSLI GKGCMC 1 cut(s) 760
BstV1I GCAGC 9 cut(s) 33, 152, 750, 939, 945, 967, 1218, 1240, 1251
BstXI CCANNNNNNTGG 3 cut(s) 335, 360, 1250
BstZ17I GTATAC 1 cut(s) 665
Bsu15I ATCGAT 1 cut(s) 1056
BsuI GTATCC 1 cut(s) 1452
BsuRI GGCC 7 cut(s) 244, 352, 388, 484, 970, 1142, 1210
BsuTUI ATCGAT 1 cut(s) 1056
BtgI CCRYGG 2 cut(s) 280, 328
BtsCI GGATG 3 cut(s) 426, 496, 592
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 3 cut(s) 878, 1132, 1365
Cac8I GCNNGC 5 cut(s) 252, 256, 266, 1016, 1086
CfoI GCGC 4 cut(s) 366, 579, 1049, 1236
Cfr10I RCCGGY 2 cut(s) 192, 300
Cfr13I GGNCC 2 cut(s) 407, 683
ClaI ATCGAT 1 cut(s) 1056
Csp6I GTAC 1 cut(s) 1357
CspAI ACCGGT 1 cut(s) 300
CviAII CATG 4 cut(s) 227, 329, 1019, 1244
CviQI GTAC 1 cut(s) 1357
DdeI CTNAG 6 cut(s) 260, 528, 540, 1102, 1125, 1268
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 2 cut(s) 350, 482
Eam1104I CTCTTC 4 cut(s) 237, 448, 745, 1444
EarI CTCTTC 4 cut(s) 237, 448, 745, 1444
EciI GGCGGA 1 cut(s) 1236
Ecl136II GAGCTC 2 cut(s) 254, 1088
Eco130I CCWWGG 1 cut(s) 328
Eco147I AGGCCT 1 cut(s) 970
Eco24I GRGCYC 4 cut(s) 256, 949, 1081, 1090
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 407, 683
Eco47III AGCGCT 1 cut(s) 1048
Eco53kI GAGCTC 2 cut(s) 254, 1088
Eco57I CTGAAG 1 cut(s) 854
EcoICRI GAGCTC 2 cut(s) 254, 1088
EcoRII CCWGG 2 cut(s) 484, 696
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 4 cut(s) 256, 949, 1081, 1090
ErhI CCWWGG 1 cut(s) 328
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 4 cut(s) 230, 332, 1022, 1247
FalI AAGNNNNNCTT 4 cut(s) 484, 516, 913, 945
FatI CATG 4 cut(s) 226, 328, 1018, 1243
FauI CCCGC 1 cut(s) 257
FblI GTMKAC 1 cut(s) 664
FokI GGATG 3 cut(s) 413, 483, 579
FriOI GRGCYC 4 cut(s) 256, 949, 1081, 1090
FspBI CTAG 2 cut(s) 966, 1489
FspI TGCGCA 1 cut(s) 578
GlaI GCGC 4 cut(s) 365, 578, 1048, 1235
GsaI CCCAGC 2 cut(s) 763, 1416
HaeII RGCGCY 1 cut(s) 1050
HaeIII GGCC 7 cut(s) 244, 352, 388, 484, 970, 1142, 1210
HapII CCGG 4 cut(s) 193, 301, 319, 1194
HhaI GCGC 4 cut(s) 366, 579, 1049, 1236
Hin1II CATG 4 cut(s) 230, 332, 1022, 1247
Hin6I GCGC 4 cut(s) 364, 577, 1047, 1234
HinP1I GCGC 4 cut(s) 364, 577, 1047, 1234
HindIII AAGCTT 3 cut(s) 355, 649, 866
HinfI GANTC 5 cut(s) 90, 322, 643, 737, 886
HpaII CCGG 4 cut(s) 193, 301, 319, 1194
HphI GGTGA 4 cut(s) 182, 290, 1010, 1294
Hpy166II GTNNAC 5 cut(s) 665, 686, 880, 1024, 1359
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 5 cut(s) 665, 686, 880, 1024, 1359
Hpy99I CGWCG 1 cut(s) 1112
HpyAV CCTTC 2 cut(s) 510, 865
HpyCH4III ACNGT 3 cut(s) 407, 803, 998
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 8 cut(s) 28, 49, 398, 766, 933, 955, 990, 1137
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 171, 247, 363, 585, 804, 930, 1085, 1273
HpyF3I CTNAG 6 cut(s) 260, 528, 540, 1102, 1125, 1268
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 230, 332, 1022, 1247
HspAI GCGC 4 cut(s) 364, 577, 1047, 1234
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 1 cut(s) 220
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 1152
Lsp1109I GCAGC 9 cut(s) 33, 152, 750, 939, 945, 967, 1218, 1240, 1251
MaeI CTAG 2 cut(s) 966, 1489
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 4 cut(s) 13, 32, 303, 1282
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 9 cut(s) 224, 435, 473, 732, 850, 1303, 1345, 1431, 1490
MhlI GDGCHC 5 cut(s) 256, 760, 949, 1081, 1090
MluCI AATT 7 cut(s) 532, 746, 823, 1371, 1387, 1407, 1469
MlyI GAGTC 4 cut(s) 84, 316, 731, 895
MmeI TCCRAC 2 cut(s) 1130, 1166
MroI TCCGGA 1 cut(s) 318
MseI TTAA 2 cut(s) 840, 864
MslI CAYNNNNRTG 3 cut(s) 333, 771, 1248
MspA1I CMGCKG 2 cut(s) 763, 1231
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 4 cut(s) 193, 301, 319, 1194
MspR9I CCNGG 3 cut(s) 486, 698, 1195
Mva1269I GAATGC 1 cut(s) 1195
MvaI CCWGG 2 cut(s) 486, 698
MwoI GCNNNNNNNGC 9 cut(s) 52, 171, 247, 363, 585, 804, 930, 1085, 1273
NciI CCSGG 1 cut(s) 1195
NcoI CCATGG 1 cut(s) 328
NdeII GATC 1 cut(s) 220
NlaIII CATG 4 cut(s) 230, 332, 1022, 1247
NlaIV GGNNCC 5 cut(s) 100, 272, 340, 684, 757
NmeAIII GCCGAG 4 cut(s) 217, 226, 364, 1186
NmuCI GTSAC 2 cut(s) 32, 1282
NsbI TGCGCA 1 cut(s) 578
NspI RCATGY 1 cut(s) 1022
PceI AGGCCT 1 cut(s) 970
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 1081
PctI GAATGC 1 cut(s) 1195
PfeI GAWTC 1 cut(s) 643
PinAI ACCGGT 1 cut(s) 300
PleI GAGTC 4 cut(s) 84, 316, 731, 894
PpsI GAGTC 4 cut(s) 84, 316, 731, 894
Psp124BI GAGCTC 2 cut(s) 256, 1090
Psp6I CCWGG 2 cut(s) 484, 696
PspFI CCCAGC 2 cut(s) 759, 1412
PspGI CCWGG 2 cut(s) 484, 696
PspN4I GGNNCC 5 cut(s) 100, 272, 340, 684, 757
PspPI GGNCC 2 cut(s) 407, 683
PstI CTGCAG 1 cut(s) 957
PvuII CAGCTG 2 cut(s) 763, 1231
RsaI GTAC 1 cut(s) 1358
RsaNI GTAC 1 cut(s) 1357
RseI CAYNNNNRTG 3 cut(s) 333, 771, 1248
SacI GAGCTC 2 cut(s) 256, 1090
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 2 cut(s) 840, 864
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 2 cut(s) 407, 683
SchI GAGTC 4 cut(s) 84, 316, 731, 895
ScrFI CCNGG 3 cut(s) 486, 698, 1195
SduI GDGCHC 5 cut(s) 256, 760, 949, 1081, 1090
SfcI CTRYAG 1 cut(s) 953
SinI GGWCC 2 cut(s) 407, 683
SmiMI CAYNNNNRTG 3 cut(s) 333, 771, 1248
SmlI CTYRAG 2 cut(s) 209, 863
SmoI CTYRAG 2 cut(s) 209, 863
Sse9I AATT 7 cut(s) 532, 746, 823, 1371, 1387, 1407, 1469
SseBI AGGCCT 1 cut(s) 970
SspMI CTAG 2 cut(s) 966, 1489
SstI GAGCTC 2 cut(s) 256, 1090
StuI AGGCCT 1 cut(s) 970
StyD4I CCNGG 3 cut(s) 484, 696, 1193
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 3 cut(s) 407, 803, 998
TaiI ACGT 1 cut(s) 460
TaqI TCGA 3 cut(s) 634, 810, 1056
TasI AATT 7 cut(s) 532, 746, 823, 1371, 1387, 1407, 1469
TauI GCSGC 4 cut(s) 244, 352, 484, 1221
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 2 cut(s) 840, 864
Tru9I TTAA 2 cut(s) 840, 864
TscAI CASTG 3 cut(s) 878, 1139, 1372
TseFI GTSAC 2 cut(s) 32, 1282
TseI GCWGC 9 cut(s) 46, 165, 763, 933, 952, 955, 1228, 1231, 1264
Tsp45I GTSAC 2 cut(s) 32, 1282
TspDTI ATGAA 2 cut(s) 800, 1381
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 3 cut(s) 878, 1139, 1372
Vha464I CTTAAG 1 cut(s) 863
VpaK11BI GGWCC 2 cut(s) 407, 683
XapI RAATTY 5 cut(s) 532, 746, 1371, 1387, 1469
XceI RCATGY 1 cut(s) 1022
XmiI GTMKAC 1 cut(s) 664
XspI CTAG 2 cut(s) 966, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.