Rh2CG068600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0022526
Physical Location & Seq
Forward (+)
5663098 .. 5663763
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG068600.1

Sequence Viewer

Length: 666 bp
ATGGTGGTGGTACGAGATCATGATCGTCTTGTGTGGCCTCCTGGTGGACCCCAAAGCAACCGTCGCCTCGATGGGGGTGCTTATCCAAACGACGTCGTTGATCTACGTGTTCCCGTCGTCTCTCGGGTTCGCCGTGTCCACCCGGGTCGGCAACGAGCTCGGGGCGAACCGGCCCAACCGGGCCAGGCTATCCGCTGTGGTCGCCATTCTCCTGGCCTTCCTCATGGGCCTATCCGCCACGACCTTCGCGTCCGGGATGAGATCAGGGCTGTGGGGCCGGATGTTCACCCACGACGCGGAGATCATACGGCTGACGTCTGCCGCGCTTCCAATCCTAGGGCTCTGCGAGCTCGGCAACTGCCCGCAGACGGTCGCGTGTGGTGTACTCAGGGGAAGCGCGCGTCCGACTACCGCAGCCAACGTGAACCTCAGCGCGTTCTATCTGGTGGGGATGCCCGTGGCGGTTGGGCTCGGGTTCTGGGTCGGAGTCGGGTTCAATGGACTCTGGATCGGGTTGCTGTCGGCCCAGATTTGTTGTGCCGGGCTGATGTTGTATGTTGTTGGGACCACGGACTGGGATTTCCAGGCGAAAAGGGCCCAGGGGCTATCGTGCGCCGGGTGCGATGTGATACTACCTCCTCCTCCTCCTTCAACTTCAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.5

Weight (kDa)

11.22

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022526)

Species Orthologous Gene IDs
pyrus_communis pycom01g20730
rosa_samantha Rh2BG067300 Rh2CG068600 Rh2DG066900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 2 cut(s) 96, 318
AccB7I CCANNNNNTGG 1 cut(s) 574
AccII CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
AciI CCGC 7 cut(s) 193, 235, 297, 322, 363, 412, 462
AclWI GGATC 1 cut(s) 516
AcyI GRCGYC 2 cut(s) 93, 315
AfaI GTAC 2 cut(s) 12, 385
AfiI CCNNNNNNNGG 6 cut(s) 44, 73, 296, 336, 368, 574
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 3 cut(s) 497, 652, 658
AjnI CCWGG 5 cut(s) 40, 183, 211, 583, 598
AluBI AGCT 2 cut(s) 158, 350
AluI AGCT 2 cut(s) 158, 350
Alw21I GWGCWC 2 cut(s) 160, 352
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 1 cut(s) 516
Ama87I CYCGRG 4 cut(s) 123, 142, 159, 471
AoxI GGCC 8 cut(s) 35, 171, 181, 214, 227, 275, 523, 595
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 1 cut(s) 414
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AspA2I CCTAGG 1 cut(s) 335
AspLEI GCGC 5 cut(s) 326, 399, 401, 435, 615
AspS9I GGNCC 9 cut(s) 47, 172, 181, 227, 275, 524, 565, 595, 596
AsuC2I CCSGG 6 cut(s) 143, 144, 180, 254, 542, 617
AsuHPI GGTGA 1 cut(s) 278
AvaI CYCGRG 4 cut(s) 123, 142, 159, 471
AvaII GGWCC 2 cut(s) 47, 565
AvrII CCTAGG 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 599
BanII GRGCYC 5 cut(s) 160, 343, 352, 472, 599
Bbv12I GWGCWC 2 cut(s) 160, 352
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 426
BccI CCATC 1 cut(s) 65
BceAI ACGGC 2 cut(s) 117, 324
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 140, 174
BciT130I CCWGG 5 cut(s) 42, 185, 213, 585, 600
BcnI CCSGG 6 cut(s) 143, 144, 180, 254, 542, 617
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 1 cut(s) 336
BisI GCNGC 2 cut(s) 322, 415
BlnI CCTAGG 1 cut(s) 335
BlsI GCNGC 2 cut(s) 323, 416
Bme18I GGWCC 2 cut(s) 47, 565
BmeT110I CYCGRG 4 cut(s) 123, 142, 159, 471
BmgT120I GGNCC 9 cut(s) 47, 172, 181, 227, 275, 524, 565, 595, 596
BmiI GGNNCC 4 cut(s) 49, 276, 566, 597
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 1 cut(s) 442
BmuI ACTGGG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 429
BpuMI CCSGG 6 cut(s) 143, 144, 180, 254, 542, 617
BsaAI YACGTR 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 93, 315
BsaJI CCNNGG 6 cut(s) 142, 335, 457, 568, 598, 599
Bsc4I CCNNNNNNNGG 6 cut(s) 44, 73, 296, 336, 368, 574
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 579, 661
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 5 cut(s) 42, 185, 213, 585, 600
BseDI CCNNGG 6 cut(s) 142, 335, 457, 568, 598, 599
BseGI GGATG 3 cut(s) 262, 286, 457
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 6 cut(s) 44, 73, 296, 336, 368, 574
BseMII CTCAG 2 cut(s) 401, 443
BseNI ACTGG 2 cut(s) 579, 661
BsePI GCGCGC 1 cut(s) 397
BseRI GAGGAG 3 cut(s) 628, 631, 634
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 1 cut(s) 426
Bsh1236I CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 8 cut(s) 37, 173, 183, 216, 229, 277, 525, 597
BsiEI CGRYCG 1 cut(s) 373
BsiHKAI GWGCWC 2 cut(s) 160, 352
BsiHKCI CYCGRG 4 cut(s) 123, 142, 159, 471
BsiSI CCGG 7 cut(s) 143, 170, 179, 253, 278, 541, 616
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 6 cut(s) 44, 73, 296, 336, 368, 574
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 8 cut(s) 37, 173, 183, 216, 229, 277, 525, 597
BsoBI CYCGRG 4 cut(s) 123, 142, 159, 471
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 5 cut(s) 160, 343, 352, 472, 599
Bsp143I GATC 6 cut(s) 16, 22, 100, 261, 301, 508
BspACI CCGC 7 cut(s) 193, 235, 297, 322, 363, 412, 462
BspANI GGCC 8 cut(s) 37, 173, 183, 216, 229, 277, 525, 597
BspCNI CTCAG 2 cut(s) 400, 442
BspFNI CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 4 cut(s) 49, 276, 566, 597
BspPI GGATC 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 579, 661
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 6 cut(s) 142, 335, 457, 568, 598, 599
BssHII GCGCGC 1 cut(s) 397
BssMI GATC 6 cut(s) 16, 22, 100, 261, 301, 508
BssNI GRCGYC 2 cut(s) 93, 315
BssT1I CCWWGG 1 cut(s) 335
Bst2UI CCWGG 5 cut(s) 42, 185, 213, 585, 600
Bst4CI ACNGT 2 cut(s) 62, 371
BstACI GRCGYC 2 cut(s) 93, 315
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 348, 363, 399
BstDEI CTNAG 2 cut(s) 387, 429
BstDSI CCRYGG 2 cut(s) 457, 568
BstF5I GGATG 3 cut(s) 262, 286, 457
BstFNI CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
BstHHI GCGC 5 cut(s) 326, 399, 401, 435, 615
BstKTI GATC 6 cut(s) 19, 25, 103, 264, 304, 511
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 6 cut(s) 16, 22, 100, 261, 301, 508
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 6 cut(s) 63, 201, 347, 352, 594, 619
BstNI CCWGG 5 cut(s) 42, 185, 213, 585, 600
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
BstV1I GCAGC 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 212
BsuRI GGCC 8 cut(s) 37, 173, 183, 216, 229, 277, 525, 597
BtgI CCRYGG 2 cut(s) 457, 568
BtgZI GCGATG 1 cut(s) 637
BtsCI GGATG 3 cut(s) 262, 286, 457
Cac8I GCNNGC 3 cut(s) 348, 363, 399
CciI TCATGA 1 cut(s) 19
CfoI GCGC 5 cut(s) 326, 399, 401, 435, 615
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 9 cut(s) 47, 172, 181, 227, 275, 524, 565, 595, 596
Cfr9I CCCGGG 1 cut(s) 142
CseI GACGC 3 cut(s) 238, 303, 390
Csp6I GTAC 2 cut(s) 11, 384
CviAII CATG 2 cut(s) 20, 224
CviQI GTAC 2 cut(s) 11, 384
DdeI CTNAG 2 cut(s) 387, 429
DpnI GATC 6 cut(s) 18, 24, 102, 263, 303, 510
DpnII GATC 6 cut(s) 16, 22, 100, 261, 301, 508
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 2 cut(s) 158, 350
Eco130I CCWWGG 1 cut(s) 335
Eco24I GRGCYC 5 cut(s) 160, 343, 352, 472, 599
Eco47I GGWCC 2 cut(s) 47, 565
Eco53kI GAGCTC 2 cut(s) 158, 350
Eco88I CYCGRG 4 cut(s) 123, 142, 159, 471
EcoICRI GAGCTC 2 cut(s) 158, 350
EcoO109I RGGNCCY 1 cut(s) 595
EcoRII CCWGG 5 cut(s) 40, 183, 211, 583, 598
EcoT14I CCWWGG 1 cut(s) 335
EcoT38I GRGCYC 5 cut(s) 160, 343, 352, 472, 599
ErhI CCWWGG 1 cut(s) 335
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 23, 227
FaiI YATR 4 cut(s) 21, 225, 306, 556
FaqI GGGAC 1 cut(s) 578
FatI CATG 2 cut(s) 19, 223
FauI CCCGC 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 322, 415
FokI GGATG 3 cut(s) 269, 293, 464
FriOI GRGCYC 5 cut(s) 160, 343, 352, 472, 599
Fsp4HI GCNGC 2 cut(s) 322, 415
FspBI CTAG 1 cut(s) 336
GlaI GCGC 5 cut(s) 325, 398, 400, 434, 614
GluI GCNGC 2 cut(s) 322, 415
HaeIII GGCC 8 cut(s) 37, 173, 183, 216, 229, 277, 525, 597
HapII CCGG 7 cut(s) 143, 170, 179, 253, 278, 541, 616
HgaI GACGC 3 cut(s) 238, 303, 390
HhaI GCGC 5 cut(s) 326, 399, 401, 435, 615
Hin1I GRCGYC 2 cut(s) 93, 315
Hin1II CATG 2 cut(s) 23, 227
Hin6I GCGC 5 cut(s) 324, 397, 399, 433, 613
HinP1I GCGC 5 cut(s) 324, 397, 399, 433, 613
HinfI GANTC 2 cut(s) 487, 502
HpaII CCGG 7 cut(s) 143, 170, 179, 253, 278, 541, 616
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 5 cut(s) 47, 139, 286, 384, 425
Hpy188I TCNGA 2 cut(s) 406, 486
Hpy188III TCNNGA 2 cut(s) 20, 506
Hpy8I GTNNAC 5 cut(s) 47, 139, 286, 384, 425
Hpy99I CGWCG 5 cut(s) 66, 95, 98, 119, 297
HpyAV CCTTC 3 cut(s) 227, 254, 658
HpyCH4III ACNGT 2 cut(s) 62, 371
HpyCH4IV ACGT 4 cut(s) 93, 106, 315, 421
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 201, 347, 352, 594, 619
HpyF3I CTNAG 2 cut(s) 387, 429
HpySE526I ACGT 4 cut(s) 93, 106, 315, 421
Hsp92I GRCGYC 2 cut(s) 93, 315
Hsp92II CATG 2 cut(s) 23, 227
HspAI GCGC 5 cut(s) 324, 397, 399, 433, 613
Kzo9I GATC 6 cut(s) 16, 22, 100, 261, 301, 508
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 1 cut(s) 442
MaeI CTAG 1 cut(s) 336
MaeII ACGT 4 cut(s) 93, 106, 315, 421
MalI GATC 6 cut(s) 18, 24, 102, 263, 303, 510
MboI GATC 6 cut(s) 16, 22, 100, 261, 301, 508
MhlI GDGCHC 5 cut(s) 160, 343, 352, 472, 599
MlyI GAGTC 2 cut(s) 496, 496
MmeI TCCRAC 2 cut(s) 429, 464
MnlI CCTC 8 cut(s) 48, 77, 231, 438, 646, 649, 652, 655
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 7 cut(s) 143, 170, 179, 253, 278, 541, 616
MvaI CCWGG 5 cut(s) 42, 185, 213, 585, 600
MvnI CGCG 7 cut(s) 249, 297, 324, 375, 399, 401, 435
MwoI GCNNNNNNNGC 6 cut(s) 63, 201, 347, 352, 594, 619
NciI CCSGG 6 cut(s) 143, 144, 180, 254, 542, 617
NdeII GATC 6 cut(s) 16, 22, 100, 261, 301, 508
NlaIII CATG 2 cut(s) 23, 227
NlaIV GGNNCC 4 cut(s) 49, 276, 566, 597
NmeAIII GCCGAG 1 cut(s) 331
PagI TCATGA 1 cut(s) 19
PasI CCCWGGG 1 cut(s) 599
PauI GCGCGC 1 cut(s) 397
PcsI WCGNNNNNNNCGW 2 cut(s) 130, 246
PflMI CCANNNNNTGG 1 cut(s) 574
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 2 cut(s) 323, 416
PleI GAGTC 2 cut(s) 495, 496
PpsI GAGTC 2 cut(s) 495, 496
Ppu21I YACGTR 1 cut(s) 107
Psp124BI GAGCTC 2 cut(s) 160, 352
Psp6I CCWGG 5 cut(s) 40, 183, 211, 583, 598
PspGI CCWGG 5 cut(s) 40, 183, 211, 583, 598
PspN4I GGNNCC 4 cut(s) 49, 276, 566, 597
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 9 cut(s) 47, 172, 181, 227, 275, 524, 565, 595, 596
PteI GCGCGC 1 cut(s) 397
RsaI GTAC 2 cut(s) 12, 385
RsaNI GTAC 2 cut(s) 11, 384
SacI GAGCTC 2 cut(s) 160, 352
SatI GCNGC 2 cut(s) 322, 415
Sau3AI GATC 6 cut(s) 16, 22, 100, 261, 301, 508
Sau96I GGNCC 9 cut(s) 47, 172, 181, 227, 275, 524, 565, 595, 596
SchI GAGTC 2 cut(s) 496, 496
SduI GDGCHC 5 cut(s) 160, 343, 352, 472, 599
SetI ASST 9 cut(s) 96, 109, 160, 246, 318, 352, 424, 430, 638
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 2 cut(s) 47, 565
SmaI CCCGGG 1 cut(s) 144
SsiI CCGC 7 cut(s) 193, 235, 297, 322, 363, 412, 462
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 2 cut(s) 160, 352
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 2 cut(s) 62, 371
TaiI ACGT 4 cut(s) 96, 109, 318, 424
TaqI TCGA 1 cut(s) 69
TatI WGTACW 1 cut(s) 383
TauI GCSGC 1 cut(s) 324
TseI GCWGC 1 cut(s) 414
TspGWI ACGGA 1 cut(s) 585
TspMI CCCGGG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 574
VpaK11BI GGWCC 2 cut(s) 47, 565
XmaI CCCGGG 1 cut(s) 142
XmaJI CCTAGG 1 cut(s) 335
XspI CTAG 1 cut(s) 336
ZraI GACGTC 2 cut(s) 94, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.