pycom01g23360

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0024611
Physical Location & Seq
Reverse (-)
20708314 .. 20709354
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGTGCCAATCTTTGAGACAGTTAACACATAGTTCTCGCGATGTTTCACGTGATTCAAAGCTCTCTGCTGTCCAAGTAGTATTGGTTCAATTATGCGAAAATGATAGCCCTACTGTGAGGGCAAATGCCATGACTGAAGACGGCAATGACAGAACATTCTTGGAGCACATAGTCAAAGGTATAGCTGCTGCAATGGGGATCATTGTTAATCTTCCAAAGGACGCGCAGATGGCGCGGTGGCTTCTAGATGCTGAGCTCTTCGCCATTAATTTCACAGTTCCGACAAATCAGGTATGGCAGAGAAAAGTAGCTGAAGCCGGTATTATACCAGTGCTGGTGGAGCTACTAACTTCTGGAACTTCTTTGACAAAACAGTACACTGCCATTTCGCTTATACAGCTATCAGAAAGCTCCAAAAGCTTGAGCAAGCCGATCAAGAAGCGTGCGGTTGTCCAGGGCTGCTTCTCTGAAGATTTCGGAGCTTGTGAAGCTTCCCTCGATGCGCTTTTGACATTGCTTGATGACCAAGGAATACAGCATGGCGGCAAAGTGCTCGATGAAGTGAAAGCCATCGCTCCGATTATAAAATTACTGAGTACTCAAGTTCCCAGTTTGCATGGAAAATCTCTCAAGGATTTGGAAATGATTTTTCGGGTTAATGAGTTGAATCTGAAGTATGGAACTTCAACACATATGGCATTTTTCGTTTATGAATACCTGCGAAAAGTCGGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

246

Amino Acids

26.99

Weight (kDa)

6.96

Isoelectric Point (pI)

25.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024611)

Species Orthologous Gene IDs
pyrus_communis pycom01g23360
rosa_samantha Rh1AG455300 Rh1CG427200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 726
AccII CGCG 3 cut(s) 39, 224, 235
AciI CCGC 3 cut(s) 235, 446, 543
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 4 cut(s) 156, 333, 489, 692
AcvI CACGTG 1 cut(s) 50
AfaI GTAC 2 cut(s) 377, 598
AgsI TTSAA 4 cut(s) 57, 89, 667, 687
AjnI CCWGG 1 cut(s) 453
Alw21I GWGCWC 3 cut(s) 168, 258, 555
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 1 cut(s) 206
ApeKI GCWGC 3 cut(s) 185, 188, 459
AseI ATTAAT 1 cut(s) 267
AspLEI GCGC 3 cut(s) 226, 235, 505
BanII GRGCYC 1 cut(s) 258
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 3 cut(s) 168, 258, 555
BbvI GCAGC 3 cut(s) 172, 175, 446
BccI CCATC 2 cut(s) 223, 578
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 535, 569
BciT130I CCWGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 726
BisI GCNGC 4 cut(s) 186, 189, 460, 544
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 4 cut(s) 187, 190, 461, 545
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 455
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 2 cut(s) 238, 490
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 252
BpuEI CTTGAG 3 cut(s) 442, 585, 614
BsaAI YACGTR 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 454, 526
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 329, 609
Bse3DI GCAATG 3 cut(s) 151, 198, 512
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 2 cut(s) 454, 526
BseMI GCAATG 3 cut(s) 151, 198, 512
BseMII CTCAG 2 cut(s) 243, 584
BseNI ACTGG 2 cut(s) 329, 609
BseXI GCAGC 3 cut(s) 172, 175, 446
Bsh1236I CGCG 3 cut(s) 39, 224, 235
BsiHKAI GWGCWC 3 cut(s) 168, 258, 555
BsiSI CCGG 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 10
Bsp1286I GDGCHC 3 cut(s) 168, 258, 555
Bsp143I GATC 2 cut(s) 198, 432
Bsp1720I GCTNAGC 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 39
BspACI CCGC 3 cut(s) 235, 446, 543
BspCNI CTCAG 2 cut(s) 244, 585
BspFNI CGCG 3 cut(s) 39, 224, 235
BspMI ACCTGC 1 cut(s) 726
BspPI GGATC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 263
BsrDI GCAATG 3 cut(s) 151, 198, 512
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 329, 609
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 454, 526
BssMI GATC 2 cut(s) 198, 432
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 4 cut(s) 21, 115, 277, 375
Bst6I CTCTTC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 428, 444
BstDEI CTNAG 2 cut(s) 252, 593
BstFNI CGCG 3 cut(s) 39, 224, 235
BstHHI GCGC 3 cut(s) 226, 235, 505
BstKTI GATC 2 cut(s) 201, 435
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 2 cut(s) 198, 432
BstMWI GCNNNNNNNGC 6 cut(s) 230, 232, 340, 397, 417, 488
BstNI CCWGG 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 453
BstUI CGCG 3 cut(s) 39, 224, 235
BstV1I GCAGC 3 cut(s) 172, 175, 446
BstV2I GAAGAC 1 cut(s) 144
BtgZI GCGATG 2 cut(s) 54, 556
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 336, 378
BtuMI TCGCGA 1 cut(s) 39
BveI ACCTGC 1 cut(s) 726
Cac8I GCNNGC 2 cut(s) 428, 444
CfoI GCGC 3 cut(s) 226, 235, 505
Cfr10I RCCGGY 1 cut(s) 317
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 376, 597
CviAII CATG 3 cut(s) 130, 539, 617
CviQI GTAC 2 cut(s) 376, 597
DdeI CTNAG 2 cut(s) 252, 593
DpnI GATC 2 cut(s) 200, 434
DpnII GATC 2 cut(s) 198, 432
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 4 cut(s) 156, 333, 489, 692
Eco72I CACGTG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 526
FaeI CATG 3 cut(s) 133, 542, 620
FatI CATG 3 cut(s) 129, 538, 616
FauNDI CATATG 1 cut(s) 693
Fnu4HI GCNGC 4 cut(s) 186, 189, 460, 544
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 186, 189, 460, 544
FspBI CTAG 1 cut(s) 245
GlaI GCGC 3 cut(s) 225, 234, 504
GluI GCNGC 4 cut(s) 186, 189, 460, 544
HapII CCGG 1 cut(s) 318
HgaI GACGC 1 cut(s) 230
HhaI GCGC 3 cut(s) 226, 235, 505
Hin1II CATG 3 cut(s) 133, 542, 620
Hin6I GCGC 3 cut(s) 224, 233, 503
HinP1I GCGC 3 cut(s) 224, 233, 503
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HindIII AAGCTT 2 cut(s) 418, 489
HinfI GANTC 3 cut(s) 53, 667, 732
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 1 cut(s) 318
Hpy166II GTNNAC 2 cut(s) 24, 378
Hpy188I TCNGA 8 cut(s) 282, 406, 469, 479, 579, 672, 731, 737
Hpy188III TCNNGA 4 cut(s) 38, 245, 354, 436
Hpy8I GTNNAC 2 cut(s) 24, 378
HpyCH4III ACNGT 4 cut(s) 21, 115, 277, 375
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 2 cut(s) 191, 616
HpyF10VI GCNNNNNNNGC 6 cut(s) 230, 232, 340, 397, 417, 488
HpyF3I CTNAG 2 cut(s) 252, 593
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 3 cut(s) 133, 542, 620
HspAI GCGC 3 cut(s) 224, 233, 503
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 198, 432
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 5 cut(s) 163, 340, 416, 479, 580
LpnPI CCDG 9 cut(s) 275, 320, 331, 339, 342, 440, 467, 622, 731
Lsp1109I GCAGC 3 cut(s) 172, 175, 446
LweI GCATC 2 cut(s) 238, 490
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 49
MalI GATC 2 cut(s) 200, 434
MboI GATC 2 cut(s) 198, 432
MboII GAAGA 4 cut(s) 149, 203, 250, 482
MhlI GDGCHC 3 cut(s) 168, 258, 555
MluCI AATT 3 cut(s) 89, 268, 587
MmeI TCCRAC 2 cut(s) 305, 709
MnlI CCTC 2 cut(s) 111, 506
MseI TTAA 4 cut(s) 23, 207, 267, 657
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 455
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 3 cut(s) 39, 224, 235
MwoI GCNNNNNNNGC 6 cut(s) 230, 232, 340, 397, 417, 488
NdeI CATATG 1 cut(s) 693
NdeII GATC 2 cut(s) 198, 432
NlaIII CATG 3 cut(s) 133, 542, 620
NruI TCGCGA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 53, 667, 732
PkrI GCNGC 4 cut(s) 187, 190, 461, 545
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
Ppu21I YACGTR 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 453
PspCI CACGTG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 453
PsrI GAACNNNNNNTAC 2 cut(s) 69, 101
RruI TCGCGA 1 cut(s) 39
RsaI GTAC 2 cut(s) 377, 598
RsaNI GTAC 2 cut(s) 376, 597
SacI GAGCTC 1 cut(s) 258
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 23, 207, 267, 657
SatI GCNGC 4 cut(s) 186, 189, 460, 544
Sau3AI GATC 2 cut(s) 198, 432
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 1 cut(s) 455
SduI GDGCHC 3 cut(s) 168, 258, 555
SfaNI GCATC 2 cut(s) 238, 490
SmlI CTYRAG 3 cut(s) 421, 600, 629
SmoI CTYRAG 3 cut(s) 421, 600, 629
Sse9I AATT 3 cut(s) 89, 268, 587
SsiI CCGC 3 cut(s) 235, 446, 543
SspMI CTAG 1 cut(s) 245
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 453
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 4 cut(s) 21, 115, 277, 375
TaiI ACGT 1 cut(s) 52
TaqI TCGA 2 cut(s) 498, 555
TasI AATT 3 cut(s) 89, 268, 587
TatI WGTACW 2 cut(s) 375, 596
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 3 cut(s) 53, 667, 732
Tru1I TTAA 4 cut(s) 23, 207, 267, 657
Tru9I TTAA 4 cut(s) 23, 207, 267, 657
TscAI CASTG 2 cut(s) 336, 385
TseI GCWGC 3 cut(s) 185, 188, 459
TspDTI ATGAA 2 cut(s) 573, 726
TspRI CASTG 2 cut(s) 336, 385
VspI ATTAAT 1 cut(s) 267
XbaI TCTAGA 1 cut(s) 244
XspI CTAG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.