pycom02g05400

Protein of unknown function (DUF1005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0029211
Physical Location & Seq
Forward (+)
3611169 .. 3612716
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05400.2

Sequence Viewer

Length: 774 bp
ATGGATCCATGCCCCTTCGTACGTCTCAATGTCGACTCTCTGGCTCTCAAGCTCCCCCACGCCACCAGACCCGCCGGTCCCGGAGTCCACTCCTCCACCACCCCCTGCTTCTGCCAGCTGAAGATCAAAAACTTCCCTCCCCAAACCGCCCTTCTCAACCTCTCCACTTCTGCCGGCGACTCCCCGCCCGACTCCTCCACCTCGGCTCCCGGCTTCCACGTGGACCCCACCCTGCTCCGGAGGCTTGCAGGGAAGCCCGTCGCGCTTCGGGTCTCAGTTTATACTGGTCGGATGGGCCAGACATGCGGCGTCACCAGTGGGAAGCTGCTAGGCCGAGTCCAACTCAGCATTGACCTGGACTCGGCTAGGGCTCACTCGATCGTGATCCACAGCGGGTGGGTGAAGTTAGGGAAGGACCGCGACAAGCCCTCCGCCAGGCTGCACTTGACGGTCCGAGCTGAACGAGACCCCCGGTTCGTTTTCCAGTTCGGAGGTGAGCCGGAGTGCAGCCCCGTGGTTTTCCAGATTCAGGGGCGGGACATACGACAGCCCGTTTTTAGCTGCAAGTTCAGTGCGGACCGTAACTCGAGAAACCGATCTCTCCAATCAGATTTTACCACCATTAACAGAGGATGGATGAGAACACTATCTGCTGATAGAGAAAGGCCAGGGAGGGAAAGAAAGGGGTGGATGATAACAATTCACGACCTATCCGGCTCGCCCGTGGCGGCCGCTTCCATCGATCACTCCCTTTGTACCCTCCCCCGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1005

Protein Analysis

258

Amino Acids

28.21

Weight (kDa)

10.04

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029211)

Species Orthologous Gene IDs
pyrus_communis pycom02g05400
rosa_multiflora Rmu_co8198526.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 33
AccII CGCG 2 cut(s) 263, 420
AccIII TCCGGA 1 cut(s) 237
AclWI GGATC 2 cut(s) 12, 379
AcoI YGGCCR 1 cut(s) 729
AcuI CTGAAG 1 cut(s) 140
AcvI CACGTG 1 cut(s) 220
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 3 cut(s) 21, 757, 770
AfiI CCNNNNNNNGG 5 cut(s) 237, 361, 435, 529, 765
AjnI CCWGG 3 cut(s) 354, 434, 667
AjuI GAANNNNNNNTTGG 2 cut(s) 135, 167
AluBI AGCT 5 cut(s) 52, 118, 325, 458, 561
AluI AGCT 5 cut(s) 52, 118, 325, 458, 561
Alw26I GTCTC 3 cut(s) 29, 277, 459
AlwI GGATC 2 cut(s) 12, 379
Ama87I CYCGRG 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 237
AoxI GGCC 4 cut(s) 295, 331, 665, 729
ApeKI GCWGC 4 cut(s) 325, 439, 507, 561
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 6 cut(s) 77, 223, 295, 415, 451, 577
AsuC2I CCSGG 4 cut(s) 81, 210, 472, 766
AsuHPI GGTGA 3 cut(s) 304, 412, 506
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 5 cut(s) 77, 223, 415, 451, 577
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 220
BbvI GCAGC 4 cut(s) 312, 426, 519, 548
BccI CCATC 3 cut(s) 286, 627, 746
BciT130I CCWGG 3 cut(s) 356, 436, 669
BcnI CCSGG 4 cut(s) 81, 210, 472, 766
BcoDI GTCTC 3 cut(s) 29, 277, 459
BfaI CTAG 2 cut(s) 329, 366
BisI GCNGC 7 cut(s) 307, 326, 440, 508, 562, 729, 732
BlsI GCNGC 7 cut(s) 308, 327, 441, 509, 563, 730, 733
Bme1390I CCNGG 7 cut(s) 81, 210, 356, 436, 472, 669, 766
Bme18I GGWCC 5 cut(s) 77, 223, 415, 451, 577
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 6 cut(s) 77, 223, 295, 415, 451, 577
BmiI GGNNCC 4 cut(s) 6, 79, 207, 225
BmrFI CCNGG 7 cut(s) 81, 210, 356, 436, 472, 669, 766
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpuEI CTTGAG 1 cut(s) 32
BpuMI CCSGG 4 cut(s) 81, 210, 472, 766
Bsa29I ATCGAT 1 cut(s) 741
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 2 cut(s) 277, 459
BsaJI CCNNGG 6 cut(s) 201, 470, 513, 668, 723, 764
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 4 cut(s) 190, 220, 413, 443
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 361, 435, 529, 765
Bse118I RCCGGY 2 cut(s) 74, 173
Bse1I ACTGG 3 cut(s) 289, 315, 484
Bse8I GATNNNNATC 1 cut(s) 383
BseAI TCCGGA 1 cut(s) 237
BseBI CCWGG 3 cut(s) 356, 436, 669
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 6 cut(s) 201, 470, 513, 668, 723, 764
BseGI GGATG 4 cut(s) 297, 638, 642, 696
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 5 cut(s) 237, 361, 435, 529, 765
BseMII CTCAG 2 cut(s) 288, 358
BseNI ACTGG 3 cut(s) 289, 315, 484
BseRI GAGGAG 2 cut(s) 82, 184
BseX3I CGGCCG 1 cut(s) 729
BseXI GCAGC 4 cut(s) 312, 426, 519, 548
BsgI GTGCAG 2 cut(s) 425, 526
Bsh1236I CGCG 2 cut(s) 263, 420
Bsh1285I CGRYCG 2 cut(s) 381, 732
BshFI GGCC 4 cut(s) 297, 333, 667, 731
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 2 cut(s) 381, 732
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 9 cut(s) 75, 81, 174, 210, 238, 472, 500, 714, 766
BsiWI CGTACG 1 cut(s) 19
BslFI GGGAC 2 cut(s) 63, 551
BslI CCNNNNNNNGG 5 cut(s) 237, 361, 435, 529, 765
BsmAI GTCTC 3 cut(s) 29, 277, 459
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 2 cut(s) 63, 551
BsnI GGCC 4 cut(s) 297, 333, 667, 731
Bso31I GGTCTC 2 cut(s) 277, 459
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 237
Bsp143I GATC 6 cut(s) 4, 123, 378, 384, 596, 742
BspANI GGCC 4 cut(s) 297, 333, 667, 731
BspCNI CTCAG 2 cut(s) 287, 357
BspDI ATCGAT 1 cut(s) 741
BspEI TCCGGA 1 cut(s) 237
BspFNI CGCG 2 cut(s) 263, 420
BspLI GGNNCC 4 cut(s) 6, 79, 207, 225
BspPI GGATC 2 cut(s) 12, 379
BspTNI GGTCTC 2 cut(s) 277, 459
BsrFI RCCGGY 2 cut(s) 74, 173
BsrI ACTGG 3 cut(s) 289, 315, 484
BssAI RCCGGY 2 cut(s) 74, 173
BssECI CCNNGG 6 cut(s) 201, 470, 513, 668, 723, 764
BssMI GATC 6 cut(s) 4, 123, 378, 384, 596, 742
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 356, 436, 669
Bst4CI ACNGT 2 cut(s) 451, 581
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 4 cut(s) 116, 175, 246, 719
BstDEI CTNAG 2 cut(s) 274, 344
BstDSI CCRYGG 2 cut(s) 513, 723
BstENI CCTNNNNNAGG 1 cut(s) 433
BstF5I GGATG 4 cut(s) 297, 638, 642, 696
BstFNI CGCG 2 cut(s) 263, 420
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 6 cut(s) 7, 126, 381, 387, 599, 745
BstMAI GTCTC 3 cut(s) 29, 277, 459
BstMBI GATC 6 cut(s) 4, 123, 378, 384, 596, 742
BstMCI CGRYCG 2 cut(s) 381, 732
BstMWI GCNNNNNNNGC 3 cut(s) 241, 262, 303
BstNI CCWGG 3 cut(s) 356, 436, 669
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 7 cut(s) 79, 208, 354, 434, 470, 667, 764
BstUI CGCG 2 cut(s) 263, 420
BstV1I GCAGC 4 cut(s) 312, 426, 519, 548
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 729
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 4 cut(s) 297, 333, 667, 731
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 2 cut(s) 513, 723
BtsCI GGATG 4 cut(s) 297, 638, 642, 696
BtsIMutI CAGTG 2 cut(s) 322, 577
Cac8I GCNNGC 4 cut(s) 116, 175, 246, 719
CciNI GCGGCCGC 1 cut(s) 729
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 2 cut(s) 74, 173
Cfr13I GGNCC 6 cut(s) 77, 223, 295, 415, 451, 577
ClaI ATCGAT 1 cut(s) 741
CpoI CGGWCCG 2 cut(s) 451, 577
CseI GACGC 1 cut(s) 298
Csp6I GTAC 3 cut(s) 20, 756, 769
CspI CGGWCCG 2 cut(s) 451, 577
CviAII CATG 2 cut(s) 9, 303
CviQI GTAC 3 cut(s) 20, 756, 769
DdeI CTNAG 2 cut(s) 274, 344
DpnI GATC 6 cut(s) 6, 125, 380, 386, 598, 744
DpnII GATC 6 cut(s) 4, 123, 378, 384, 596, 742
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
EaeI YGGCCR 1 cut(s) 729
EagI CGGCCG 1 cut(s) 729
EciI GGCGGA 1 cut(s) 421
EclXI CGGCCG 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 373
Eco31I GGTCTC 2 cut(s) 277, 459
Eco47I GGWCC 5 cut(s) 77, 223, 415, 451, 577
Eco52I CGGCCG 1 cut(s) 729
Eco57I CTGAAG 1 cut(s) 140
Eco72I CACGTG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 586
EcoNI CCTNNNNNAGG 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 354, 434, 667
EcoT38I GRGCYC 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 2 cut(s) 12, 306
FaiI YATR 4 cut(s) 10, 282, 304, 542
FaqI GGGAC 2 cut(s) 63, 551
FatI CATG 2 cut(s) 8, 302
FauI CCCGC 4 cut(s) 79, 192, 386, 528
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 7 cut(s) 307, 326, 440, 508, 562, 729, 732
FokI GGATG 4 cut(s) 304, 645, 649, 703
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 7 cut(s) 307, 326, 440, 508, 562, 729, 732
FspBI CTAG 2 cut(s) 329, 366
GlaI GCGC 1 cut(s) 264
GluI GCNGC 7 cut(s) 307, 326, 440, 508, 562, 729, 732
HaeIII GGCC 4 cut(s) 297, 333, 667, 731
HapII CCGG 9 cut(s) 75, 81, 174, 210, 238, 472, 500, 714, 766
HgaI GACGC 1 cut(s) 298
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 12, 306
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 7 cut(s) 35, 84, 179, 191, 336, 359, 526
HpaII CCGG 9 cut(s) 75, 81, 174, 210, 238, 472, 500, 714, 766
HphI GGTGA 3 cut(s) 304, 412, 506
Hpy166II GTNNAC 3 cut(s) 34, 88, 223
Hpy188I TCNGA 4 cut(s) 291, 455, 491, 610
Hpy188III TCNNGA 5 cut(s) 238, 382, 523, 588, 704
Hpy8I GTNNAC 3 cut(s) 34, 88, 223
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 3 cut(s) 25, 161, 406
HpyCH4III ACNGT 2 cut(s) 451, 581
HpyCH4IV ACGT 2 cut(s) 22, 219
HpyCH4V TGCA 4 cut(s) 248, 442, 507, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 241, 262, 303
HpyF3I CTNAG 2 cut(s) 274, 344
HpySE526I ACGT 2 cut(s) 22, 219
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 12, 306
HspAI GCGC 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 237
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 6 cut(s) 4, 123, 378, 384, 596, 742
LmnI GCTCC 3 cut(s) 57, 211, 240
Lsp1109I GCAGC 4 cut(s) 312, 426, 519, 548
MaeI CTAG 2 cut(s) 329, 366
MaeII ACGT 2 cut(s) 22, 219
MaeIII GTNAC 2 cut(s) 310, 581
MalI GATC 6 cut(s) 6, 125, 380, 386, 598, 744
MboI GATC 6 cut(s) 4, 123, 378, 384, 596, 742
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 1 cut(s) 699
MlyI GAGTC 6 cut(s) 29, 93, 173, 185, 345, 353
MmeI TCCRAC 2 cut(s) 269, 364
MroI TCCGGA 1 cut(s) 237
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 118, 393
MspI CCGG 9 cut(s) 75, 81, 174, 210, 238, 472, 500, 714, 766
MspR9I CCNGG 7 cut(s) 81, 210, 356, 436, 472, 669, 766
MvaI CCWGG 3 cut(s) 356, 436, 669
MvnI CGCG 2 cut(s) 263, 420
MwoI GCNNNNNNNGC 3 cut(s) 241, 262, 303
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 4 cut(s) 81, 210, 472, 766
NdeII GATC 6 cut(s) 4, 123, 378, 384, 596, 742
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 12, 306
NlaIV GGNNCC 4 cut(s) 6, 79, 207, 225
NmeAIII GCCGAG 3 cut(s) 182, 341, 359
NmuCI GTSAC 1 cut(s) 310
NotI GCGGCCGC 1 cut(s) 729
NspI RCATGY 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 586
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 526
Pfl23II CGTACG 1 cut(s) 19
PfoI TCCNGGA 1 cut(s) 79
PkrI GCNGC 7 cut(s) 308, 327, 441, 509, 563, 730, 733
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 6 cut(s) 29, 92, 173, 185, 344, 353
PmaCI CACGTG 1 cut(s) 220
PmlI CACGTG 1 cut(s) 220
PpsI GAGTC 6 cut(s) 29, 92, 173, 185, 344, 353
Ppu21I YACGTR 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 354, 434, 667
PspCI CACGTG 1 cut(s) 220
PspGI CCWGG 3 cut(s) 354, 434, 667
PspLI CGTACG 1 cut(s) 19
PspN4I GGNNCC 4 cut(s) 6, 79, 207, 225
PspPI GGNCC 6 cut(s) 77, 223, 295, 415, 451, 577
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 3 cut(s) 21, 757, 770
RsaNI GTAC 3 cut(s) 20, 756, 769
Rsr2I CGGWCCG 2 cut(s) 451, 577
RsrII CGGWCCG 2 cut(s) 451, 577
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 7 cut(s) 307, 326, 440, 508, 562, 729, 732
Sau3AI GATC 6 cut(s) 4, 123, 378, 384, 596, 742
Sau96I GGNCC 6 cut(s) 77, 223, 295, 415, 451, 577
SchI GAGTC 6 cut(s) 29, 93, 173, 185, 345, 353
ScrFI CCNGG 7 cut(s) 81, 210, 356, 436, 472, 669, 766
SduI GDGCHC 1 cut(s) 373
Sfr274I CTCGAG 1 cut(s) 586
SinI GGWCC 5 cut(s) 77, 223, 415, 451, 577
SlaI CTCGAG 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 47, 586
SmoI CTYRAG 2 cut(s) 47, 586
Sse9I AATT 1 cut(s) 699
SspMI CTAG 2 cut(s) 329, 366
StyD4I CCNGG 7 cut(s) 79, 208, 354, 434, 470, 667, 764
TaaI ACNGT 2 cut(s) 451, 581
TaiI ACGT 2 cut(s) 25, 222
TaqI TCGA 4 cut(s) 33, 377, 587, 741
TasI AATT 1 cut(s) 699
TauI GCSGC 3 cut(s) 309, 731, 734
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 2 cut(s) 322, 577
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 4 cut(s) 325, 439, 507, 561
Tsp45I GTSAC 1 cut(s) 310
TspRI CASTG 2 cut(s) 322, 577
VpaK11BI GGWCC 5 cut(s) 77, 223, 415, 451, 577
XagI CCTNNNNNAGG 1 cut(s) 433
XceI RCATGY 1 cut(s) 306
XhoI CTCGAG 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 2 cut(s) 329, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.