pycom02g11280

Uncharacterised protein family (UPF0261)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022378
Physical Location & Seq
Reverse (-)
7984833 .. 7985983
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGTGTGCGGCATTAATAGCTGAGCAGGGCCGTATTGAGCAATGCTGCTGCTGCGTTTTGTGGGATGGTGGTTGGGAGAGTTGGGAGGGTTGGGATGGTGGCTGTGGCGGGGTGGAGAAATCAACGGTGGGGCTGACCATGTTCGGGGTGACGACTCGGTGTGTGAATGGGGTGAAAGAGAGATTGGAGAAAGAAGGGTATGGAACATTGGTTTTTCATGCCACTGGGGTTGGAGGGAGAGCCATGGAGTCTCTGGTTAGAGATGGGTTCATAAAGGGTGTTTTGGATATCACAACAACAGAGGTTGCAGACAATGTGGTTTGGGGTGTCATGGCTTGTGATGCTTCCCACTTTGATGCCATCATAGAGAAAAGGATTCCTTTAGTACTAAGGGTTGGAGCTTTGGATATGGTAAACTTTGGAGCTAAGGATACCATACCATCCAATTTCAACACAGAAAGATTCATGAACTTAACAAGTTTCACTCATGAGAACCACTGTAGTCGAGAACAAGAAATTTGCTGGCTTTATAGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

180

Amino Acids

19.74

Weight (kDa)

4.98

Isoelectric Point (pI)

30.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0261_C PF23189 40 - 157 1.3e-49 Uncharacterised protein family (UPF0261) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022378)

Species Orthologous Gene IDs
malus_domestica MD02G1142800.v1.1
pyrus_communis pycom02g11230 pycom02g11280 pycom02g11310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 108
AcsI RAATTY 1 cut(s) 516
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 144
AgsI TTSAA 1 cut(s) 451
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 3 cut(s) 20, 401, 425
AluI AGCT 3 cut(s) 20, 401, 425
Alw26I GTCTC 1 cut(s) 255
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 3 cut(s) 45, 48, 51
ApoI RAATTY 1 cut(s) 516
AseI ATTAAT 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 160, 184
BbvI GCAGC 3 cut(s) 32, 35, 38
BccI CCATC 5 cut(s) 59, 89, 257, 368, 448
BceAI ACGGC 1 cut(s) 15
BciVI GTATCC 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 499
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 4 cut(s) 9, 46, 49, 52
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 4 cut(s) 10, 47, 50, 53
BmcAI AGTACT 1 cut(s) 387
BmgT120I GGNCC 1 cut(s) 28
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 331, 346
BmuI ACTGGG 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 3 cut(s) 70, 100, 440
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 32, 35, 38
BshFI GGCC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 255
BsnI GGCC 1 cut(s) 30
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 2 cut(s) 8, 108
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 1 cut(s) 13
BspHI TCATGA 2 cut(s) 465, 487
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 127, 500
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 3 cut(s) 21, 389, 426
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 3 cut(s) 70, 100, 440
BstMAI GTCTC 1 cut(s) 255
BstMWI GCNNNNNNNGC 3 cut(s) 17, 51, 341
BstSFI CTRYAG 1 cut(s) 499
BstV1I GCAGC 3 cut(s) 32, 35, 38
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 243
BtsCI GGATG 3 cut(s) 70, 100, 440
BtsIMutI CAGTG 2 cut(s) 222, 496
Cac8I GCNNGC 1 cut(s) 524
CciI TCATGA 2 cut(s) 465, 487
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 386
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 6 cut(s) 139, 218, 244, 331, 466, 488
CviJI RGCY 9 cut(s) 20, 30, 102, 133, 242, 335, 401, 425, 526
CviKI_1 RGCY 9 cut(s) 20, 30, 102, 133, 242, 335, 401, 425, 526
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 3 cut(s) 21, 389, 426
Eco130I CCWWGG 1 cut(s) 243
Eco32I GATATC 1 cut(s) 289
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 6 cut(s) 142, 221, 247, 334, 469, 491
FalI AAGNNNNNCTT 2 cut(s) 364, 396
FatI CATG 6 cut(s) 138, 217, 243, 330, 465, 487
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 4 cut(s) 9, 46, 49, 52
FokI GGATG 3 cut(s) 77, 107, 427
Fsp4HI GCNGC 4 cut(s) 9, 46, 49, 52
GluI GCNGC 4 cut(s) 9, 46, 49, 52
HaeIII GGCC 1 cut(s) 30
Hin1II CATG 6 cut(s) 142, 221, 247, 334, 469, 491
HinfI GANTC 4 cut(s) 154, 248, 376, 462
HphI GGTGA 2 cut(s) 160, 184
Hpy166II GTNNAC 1 cut(s) 415
Hpy188III TCNNGA 3 cut(s) 466, 488, 506
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 127, 500
HpyCH4V TGCA 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 51, 341
HpyF3I CTNAG 3 cut(s) 21, 389, 426
Hsp92II CATG 6 cut(s) 142, 221, 247, 334, 469, 491
LmnI GCTCC 2 cut(s) 398, 422
LpnPI CCDG 4 cut(s) 11, 210, 239, 508
Lsp1109I GCAGC 3 cut(s) 32, 35, 38
LweI GCATC 2 cut(s) 331, 346
MaeIII GTNAC 1 cut(s) 148
MluCI AATT 2 cut(s) 445, 516
MlyI GAGTC 2 cut(s) 148, 257
MmeI TCCRAC 2 cut(s) 211, 376
MnlI CCTC 3 cut(s) 79, 227, 295
MseI TTAA 2 cut(s) 14, 473
MslI CAYNNNNRTG 1 cut(s) 354
MwoI GCNNNNNNNGC 3 cut(s) 17, 51, 341
NcoI CCATGG 1 cut(s) 243
NlaIII CATG 6 cut(s) 142, 221, 247, 334, 469, 491
NmuCI GTSAC 1 cut(s) 148
PagI TCATGA 2 cut(s) 465, 487
PfeI GAWTC 2 cut(s) 376, 462
PkrI GCNGC 4 cut(s) 10, 47, 50, 53
PleI GAGTC 2 cut(s) 148, 256
PpsI GAGTC 2 cut(s) 148, 256
PshBI ATTAAT 1 cut(s) 14
PspPI GGNCC 1 cut(s) 28
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 354
SaqAI TTAA 2 cut(s) 14, 473
SatI GCNGC 4 cut(s) 9, 46, 49, 52
Sau96I GGNCC 1 cut(s) 28
ScaI AGTACT 1 cut(s) 387
SchI GAGTC 2 cut(s) 148, 257
SetI ASST 4 cut(s) 22, 306, 403, 427
SfaNI GCATC 2 cut(s) 331, 346
SfcI CTRYAG 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 354
Sse9I AATT 2 cut(s) 445, 516
SsiI CCGC 2 cut(s) 8, 108
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 127, 500
TaqI TCGA 1 cut(s) 505
TasI AATT 2 cut(s) 445, 516
TatI WGTACW 1 cut(s) 385
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 376, 462
Tru1I TTAA 2 cut(s) 14, 473
Tru9I TTAA 2 cut(s) 14, 473
TscAI CASTG 2 cut(s) 229, 503
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 45, 48, 51
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 206, 259, 454, 482
TspRI CASTG 2 cut(s) 229, 503
VspI ATTAAT 1 cut(s) 14
XapI RAATTY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
ZrmI AGTACT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.