pycom02g15830

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0024363
Physical Location & Seq
Forward (+)
12808248 .. 12808859
612 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGAGGGGCCGCAAAAAGATTCAAATGGAGTTGATTGAGAGTCAACATGCACGGCAAGTCTCTTTCTCCAAACGCAGATCCAGCCTCTTCAAAAAGGCTAGTGAGCTTTGCACCATGTGTGCTGTTCAGGTTTTACTGGTTATCTTCTCACTTGGAAGCAAGCCCTTTTCTTTTGGCTACCCTTCCGTTCATTCGGTACTTAACAGGTTTCATCAGTTTCAAAACCCTGATGCCAGTACCAGTCAAAGCGAGGCTACAAAAGAACCAGTTATGCAAGGTCTTAACGACAGCTTCTCTCATTTACTTGAACAAGTGAAATTTGAGCAGAAAAGGGGAAAGCAACTGAACAGGAAAATAATGGAGCATTCGGGGCCTGATTGGTTAAAAGCACCAATGGATAGCCTCAACCTCGAGGAGTTACGCAAGGTCAAGGAAACTCTTGTCGATGTAAAAAAGAAGATAAAAAAGCAAAGGGATGAGCTTCTAGCGGAACCTCTTGCATCCTCAGCCAATAATTCTATAGAGTTGGCTCATTGTCCTGAAACTAACCTAGTTGGAGACATTTCTTCTGTTCATCCTCAAGGCTCATTAACTTTTAGGGAGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

204

Amino Acids

23.07

Weight (kDa)

9.11

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 12 - 58 1.4e-19 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024363)

Species Orthologous Gene IDs
malus_domestica MD02G1194300.v1.1
prunus_persica Prupe.6G213000_v2.0.a1
pyrus_communis pycom02g15830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 410
AciI CCGC 2 cut(s) 10, 488
AclWI GGATC 1 cut(s) 72
AcsI RAATTY 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 117
AfaI GTAC 2 cut(s) 198, 238
AgsI TTSAA 4 cut(s) 23, 91, 221, 308
AluBI AGCT 3 cut(s) 106, 291, 481
AluI AGCT 3 cut(s) 106, 291, 481
Alw26I GTCTC 2 cut(s) 64, 552
AlwI GGATC 1 cut(s) 72
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 2 cut(s) 7, 371
ApoI RAATTY 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 7, 371
AvaI CYCGRG 1 cut(s) 410
BbvCI CCTCAGC 1 cut(s) 505
BceAI ACGGC 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 64, 552
BfaI CTAG 3 cut(s) 99, 485, 551
BfmI CTRYAG 1 cut(s) 519
BisI GCNGC 1 cut(s) 10
BlsI GCNGC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 7, 371
BmiI GGNNCC 3 cut(s) 8, 372, 492
BmsI GCATC 2 cut(s) 220, 509
Bpu10I CCTNAGC 1 cut(s) 505
BpuEI CTTGAG 1 cut(s) 564
BsaXI ACNNNNNCTCC 1 cut(s) 593
Bse1I ACTGG 4 cut(s) 141, 234, 240, 266
BseGI GGATG 3 cut(s) 481, 500, 574
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 4 cut(s) 141, 234, 240, 266
BseRI GAGGAG 1 cut(s) 428
BshFI GGCC 2 cut(s) 9, 373
BsiHKCI CYCGRG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 64, 552
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 9, 373
BsoBI CYCGRG 1 cut(s) 410
Bsp143I GATC 1 cut(s) 77
BspACI CCGC 2 cut(s) 10, 488
BspANI GGCC 2 cut(s) 9, 373
BspCNI CTCAG 1 cut(s) 518
BspLI GGNNCC 3 cut(s) 8, 372, 492
BspPI GGATC 1 cut(s) 72
BsrI ACTGG 4 cut(s) 141, 234, 240, 266
BssMI GATC 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 92, 600
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 1 cut(s) 505
BstF5I GGATG 3 cut(s) 481, 500, 574
BstKTI GATC 1 cut(s) 80
BstMAI GTCTC 2 cut(s) 64, 552
BstMBI GATC 1 cut(s) 77
BstMWI GCNNNNNNNGC 3 cut(s) 81, 370, 506
BstNSI RCATGY 1 cut(s) 50
BstSFI CTRYAG 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 2 cut(s) 9, 373
BtsCI GGATG 3 cut(s) 481, 500, 574
Cac8I GCNNGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 7, 371
Csp6I GTAC 2 cut(s) 197, 237
CviAII CATG 2 cut(s) 47, 115
CviQI GTAC 2 cut(s) 197, 237
DdeI CTNAG 1 cut(s) 505
DpnI GATC 1 cut(s) 79
DpnII GATC 1 cut(s) 77
DraIII CACNNNGTG 1 cut(s) 117
Eam1104I CTCTTC 2 cut(s) 92, 600
EarI CTCTTC 2 cut(s) 92, 600
Eco88I CYCGRG 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 371
FaeI CATG 2 cut(s) 50, 118
FaiI YATR 4 cut(s) 48, 116, 272, 521
FatI CATG 2 cut(s) 46, 114
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 3 cut(s) 487, 488, 561
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 3 cut(s) 99, 485, 551
GluI GCNGC 1 cut(s) 10
HaeIII GGCC 2 cut(s) 9, 373
Hin1II CATG 2 cut(s) 50, 118
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HinfI GANTC 2 cut(s) 19, 40
Hpy166II GTNNAC 1 cut(s) 44
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 50, 111, 274, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 370, 506
HpyF3I CTNAG 1 cut(s) 505
Hsp92II CATG 2 cut(s) 50, 118
Kzo9I GATC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 361
LweI GCATC 2 cut(s) 220, 509
MaeI CTAG 3 cut(s) 99, 485, 551
MaeIII GTNAC 1 cut(s) 417
MalI GATC 1 cut(s) 79
MboI GATC 1 cut(s) 77
MboII GAAGA 4 cut(s) 79, 136, 469, 558
MflI RGATCY 1 cut(s) 77
MluCI AATT 2 cut(s) 317, 514
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 9 cut(s) 95, 244, 406, 413, 419, 504, 514, 588, 595
MseI TTAA 4 cut(s) 201, 282, 383, 590
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 3 cut(s) 81, 370, 506
NdeII GATC 1 cut(s) 77
NlaIII CATG 2 cut(s) 50, 118
NlaIV GGNNCC 3 cut(s) 8, 372, 492
NspI RCATGY 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 410
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 1 cut(s) 11
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PspN4I GGNNCC 3 cut(s) 8, 372, 492
PspPI GGNCC 2 cut(s) 7, 371
PspXI VCTCGAGB 1 cut(s) 410
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 2 cut(s) 198, 238
RsaNI GTAC 2 cut(s) 197, 237
SaqAI TTAA 4 cut(s) 201, 282, 383, 590
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 7, 371
SchI GAGTC 1 cut(s) 49
SfaNI GCATC 2 cut(s) 220, 509
SfcI CTRYAG 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 410
SmlI CTYRAG 2 cut(s) 410, 579
SmoI CTYRAG 2 cut(s) 410, 579
Sse9I AATT 2 cut(s) 317, 514
SsiI CCGC 2 cut(s) 10, 488
SspMI CTAG 3 cut(s) 99, 485, 551
TaqI TCGA 2 cut(s) 411, 444
TasI AATT 2 cut(s) 317, 514
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 4 cut(s) 201, 282, 383, 590
Tru9I TTAA 4 cut(s) 201, 282, 383, 590
TspDTI ATGAA 3 cut(s) 179, 200, 563
TspGWI ACGGA 1 cut(s) 175
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 410
XspI CTAG 3 cut(s) 99, 485, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.