pycom02g25380

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0029226
Physical Location & Seq
Forward (+)
23339869 .. 23340797
929 bp
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UTR
Exon/CDS
Intron
pycom02g25380.1

Sequence Viewer

Length: 267 bp
ATGCCAGAAGACAAGAGGTTCAATGGAAAGAAGGCTTTATCTGAAGCATCTGTTATCGTGAGGGAGCATACACCATTGACTAATATGTTTATGTGCCTCTGGTTCAACGAGGTGGTTCAATTCACTTCTCGTGATCAGCTTAGTTTCCCATATGTTTTATGGCGGCTGAAAGTGCTTGAGAACGTCAACGTGTTCCCTGTGTGTACCCGGAAAGATCTCGTTAATAGTATGGGTCACATACGCAAGGCTAAGCCACTCGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

89

Amino Acids

10.24

Weight (kDa)

9.62

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 8 - 79 7.9e-28 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029226)

Species Orthologous Gene IDs
pyrus_communis pycom02g25380
rosa_multiflora Rmu_sc0001296.1_g000018

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 3 cut(s) 22, 106, 119
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
AsuC2I CCSGG 1 cut(s) 208
BauI CACGAG 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 15
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 1 cut(s) 208
BglII AGATCT 1 cut(s) 214
BisI GCNGC 1 cut(s) 164
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 15
Bpu1102I GCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 208
BsiSI CCGG 1 cut(s) 208
Bsp143I GATC 2 cut(s) 133, 214
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 1 cut(s) 163
BssMI GATC 2 cut(s) 133, 214
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 140, 249
BstKTI GATC 2 cut(s) 136, 217
BstMBI GATC 2 cut(s) 133, 214
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
Csp6I GTAC 1 cut(s) 204
CviJI RGCY 5 cut(s) 35, 139, 166, 248, 253
CviKI_1 RGCY 5 cut(s) 35, 139, 166, 248, 253
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 2 cut(s) 140, 249
DpnI GATC 2 cut(s) 135, 216
DpnII GATC 2 cut(s) 133, 214
Eco57I CTGAAG 1 cut(s) 63
FaiI YATR 8 cut(s) 69, 86, 92, 151, 153, 160, 230, 239
FauNDI CATATG 1 cut(s) 151
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 164
HapII CCGG 1 cut(s) 208
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HpaII CCGG 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 187, 204
Hpy188I TCNGA 2 cut(s) 43, 260
Hpy188III TCNNGA 2 cut(s) 58, 131
Hpy8I GTNNAC 2 cut(s) 187, 204
HpyAV CCTTC 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 183, 189
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 140, 249
HpySE526I ACGT 2 cut(s) 183, 189
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 2 cut(s) 133, 214
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 4 cut(s) 18, 85, 210, 221
LweI GCATC 1 cut(s) 56
MaeII ACGT 2 cut(s) 183, 189
MaeIII GTNAC 1 cut(s) 233
MalI GATC 2 cut(s) 135, 216
MboI GATC 2 cut(s) 133, 214
MboII GAAGA 1 cut(s) 20
MflI RGATCY 1 cut(s) 214
MluCI AATT 1 cut(s) 119
MnlI CCTC 4 cut(s) 9, 54, 103, 107
MseI TTAA 1 cut(s) 222
MspI CCGG 1 cut(s) 208
MspR9I CCNGG 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 172
NciI CCSGG 1 cut(s) 208
NdeI CATATG 1 cut(s) 151
NdeII GATC 2 cut(s) 133, 214
NmuCI GTSAC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 165
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 2 cut(s) 133, 214
ScrFI CCNGG 1 cut(s) 208
SetI ASST 5 cut(s) 20, 114, 141, 186, 192
SfaNI GCATC 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 119
SsiI CCGC 1 cut(s) 163
StyD4I CCNGG 1 cut(s) 206
TaiI ACGT 2 cut(s) 186, 192
TasI AATT 1 cut(s) 119
TauI GCSGC 1 cut(s) 166
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseFI GTSAC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.