pycom03g00630

Protein TRANSPORT INHIBITOR RESPONSE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0024448
Physical Location & Seq
Forward (+)
516149 .. 517538
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00630.3

Sequence Viewer

Length: 840 bp
ATGGGTTCTCAGAGCAAGGTTAATCTACTTGGATTCAAACTTAATTATCTTAACATTTATACCTATATCATTAACTGGGAATATTTGCTGTACGGGGAAGAAAATTGCCTCAACCTAGCCCCTAATGTTAGCATCGATAGTCCTTACAGATTTCCAGATGATTTCAACTGGCGTATCAGGATAGATTTAAGGACTGGGAATCTTCACGTTTTGAGATTGTGCCGCTGCTGTGTTATGCGGGCAGAAGCAGATGCTGAGATCGCAGGGCCTCAGGAAGATCTTACTGGCCTCGGTCTTGGCCATGGTGGCGGAGACGACGTTTCCGGTAGACGTTATGAAGACTTCCATTTTGCTGCGATAATGACTGCTCCAACGATCATGGATTCAATTGGGGGAAATGCCGAAAATGGCAAACTCGTTTCTCGAGGAGGTGCTGGACCATGTGTTCTCATTCATATAGACGGACCAGGACCGGAACTTGATCTCGATAGTGTGTGCAAGTTGTGGTACGAGATCAAACGATGGTGCTGGCGGCGGATTTTCGGTAGAAACTGCTATGCCGTCAGCCCTAAGATAGTGATAAGGCGATTCCTGGACGTTAGATTCATCGGGCTCAAGGGACTGCCTCCATGCGCAGACTCTTCAATCTGGTTCCCCGAGATTGGGTTGGTACGTGTACCCTTCGATCGCCGTCACTGCTACCAATTGCAGGTTTCTCTCTCTTGGGTTCTCGGTGCATTCTTCACCATCTCCAATGGTATCTCCAGGTACTTGTATACTATCGTTGAACATATTTCTTCCAGCCATGAACATGCACTTACATATACGATAATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000822 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006464 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007584 GO:0008144 GO:0008150 GO:0008152 GO:0009267 GO:0009555 GO:0009605 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0009991 GO:0010011 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010152 GO:0010311 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0016567 GO:0016740 GO:0019005 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0036094 GO:0036211 GO:0038023 GO:0038198 GO:0042221 GO:0042562 GO:0042594 GO:0043167 GO:0043168 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045013 GO:0045014 GO:0045892 GO:0045934 GO:0045990 GO:0046015 GO:0048229 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060089 GO:0060255 GO:0061458 GO:0061984 GO:0061985 GO:0061986 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071496 GO:0071704 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1905393 GO:1990234 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

280

Amino Acids

31.6

Weight (kDa)

6.45

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024448)

Species Orthologous Gene IDs
malus_domestica MD17G1124500.v1.1
pyrus_communis pycom03g00630 pycom04g08810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 700
AccI GTMKAC 2 cut(s) 328, 776
AciI CCGC 5 cut(s) 223, 238, 309, 532, 535
AcoI YGGCCR 1 cut(s) 298
AfaI GTAC 5 cut(s) 92, 509, 672, 678, 770
AfiI CCNNNNNNNGG 3 cut(s) 662, 663, 709
AflIII ACRYGT 1 cut(s) 673
AgsI TTSAA 6 cut(s) 37, 166, 387, 645, 788, 836
AjnI CCWGG 3 cut(s) 466, 591, 764
AloI GAACNNNNNNTCC 2 cut(s) 429, 461
Alw26I GTCTC 1 cut(s) 306
AlwNI CAGNNNCTG 1 cut(s) 254
Ama87I CYCGRG 2 cut(s) 423, 656
AoxI GGCC 3 cut(s) 266, 286, 298
ApeKI GCWGC 2 cut(s) 225, 353
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 4 cut(s) 266, 437, 464, 470
AsuHPI GGTGA 1 cut(s) 736
AvaI CYCGRG 2 cut(s) 423, 656
AvaII GGWCC 3 cut(s) 437, 464, 470
AxyI CCTNAGG 1 cut(s) 270
BaeI ACNNNNGTAYC 2 cut(s) 157, 190
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 615
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 212, 340
BccI CCATC 2 cut(s) 516, 755
BceAI ACGGC 2 cut(s) 545, 675
BciT130I CCWGG 3 cut(s) 468, 593, 766
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 700
BglI GCCNNNNNGGC 1 cut(s) 306
BglII AGATCT 1 cut(s) 277
BisI GCNGC 4 cut(s) 223, 226, 354, 533
BlsI GCNGC 4 cut(s) 224, 227, 355, 534
Bme1390I CCNGG 3 cut(s) 468, 593, 766
Bme18I GGWCC 3 cut(s) 437, 464, 470
BmeT110I CYCGRG 2 cut(s) 423, 656
BmgT120I GGNCC 4 cut(s) 266, 437, 464, 470
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 3 cut(s) 468, 593, 766
BmrI ACTGGG 2 cut(s) 85, 204
BmsI GCATC 2 cut(s) 141, 241
BmuI ACTGGG 2 cut(s) 85, 204
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 748
BpuEI CTTGAG 1 cut(s) 599
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 674
BsaJI CCNNGG 2 cut(s) 289, 301
BsaWI WCCGGW 2 cut(s) 323, 472
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 3 cut(s) 662, 663, 709
Bse1I ACTGG 4 cut(s) 80, 173, 199, 289
Bse21I CCTNAGG 1 cut(s) 270
BseBI CCWGG 3 cut(s) 468, 593, 766
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 289, 301
BseLI CCNNNNNNNGG 3 cut(s) 662, 663, 709
BseMII CTCAG 3 cut(s) 23, 246, 284
BseNI ACTGG 4 cut(s) 80, 173, 199, 289
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 2 cut(s) 212, 340
Bsh1285I CGRYCG 1 cut(s) 688
BshFI GGCC 3 cut(s) 268, 288, 300
BshVI ATCGAT 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 688
BsiHKCI CYCGRG 2 cut(s) 423, 656
BsiSI CCGG 2 cut(s) 324, 473
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 3 cut(s) 662, 663, 709
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 3 cut(s) 268, 288, 300
BsoBI CYCGRG 2 cut(s) 423, 656
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 6 cut(s) 258, 277, 375, 481, 513, 685
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 5 cut(s) 223, 238, 309, 532, 535
BspANI GGCC 3 cut(s) 268, 288, 300
BspCNI CTCAG 3 cut(s) 22, 247, 283
BspDI ATCGAT 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 653
BspMI ACCTGC 1 cut(s) 700
BsrI ACTGG 4 cut(s) 80, 173, 199, 289
BssECI CCNNGG 2 cut(s) 289, 301
BssMI GATC 6 cut(s) 258, 277, 375, 481, 513, 685
BssNAI GTATAC 1 cut(s) 777
BssT1I CCWWGG 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 777
Bst2UI CCWGG 3 cut(s) 468, 593, 766
Bst6I CTCTTC 1 cut(s) 646
BstBAI YACGTR 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 240, 530
BstDEI CTNAG 4 cut(s) 9, 255, 270, 570
BstDSI CCRYGG 1 cut(s) 301
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 6 cut(s) 261, 280, 378, 484, 516, 688
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 6 cut(s) 258, 277, 375, 481, 513, 685
BstMCI CGRYCG 1 cut(s) 688
BstMWI GCNNNNNNNGC 3 cut(s) 260, 306, 696
BstNI CCWGG 3 cut(s) 468, 593, 766
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 3 cut(s) 466, 591, 764
BstV1I GCAGC 2 cut(s) 212, 340
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BstZ17I GTATAC 1 cut(s) 777
Bsu15I ATCGAT 1 cut(s) 135
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 268, 288, 300
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 301
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 694
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 2 cut(s) 240, 530
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 1 cut(s) 635
Cfr13I GGNCC 4 cut(s) 266, 437, 464, 470
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 5 cut(s) 91, 508, 671, 677, 769
CviAII CATG 6 cut(s) 302, 379, 441, 630, 806, 812
CviJI RGCY 7 cut(s) 119, 268, 288, 300, 567, 613, 804
CviKI_1 RGCY 7 cut(s) 119, 268, 288, 300, 567, 613, 804
CviQI GTAC 5 cut(s) 91, 508, 671, 677, 769
DdeI CTNAG 4 cut(s) 9, 255, 270, 570
DpnI GATC 6 cut(s) 260, 279, 377, 483, 515, 687
DpnII GATC 6 cut(s) 258, 277, 375, 481, 513, 685
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 646
EarI CTCTTC 1 cut(s) 646
EciI GGCGGA 2 cut(s) 324, 550
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 615
Eco47I GGWCC 3 cut(s) 437, 464, 470
Eco81I CCTNAGG 1 cut(s) 270
Eco88I CYCGRG 2 cut(s) 423, 656
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 466, 591, 764
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 6 cut(s) 305, 382, 444, 633, 809, 815
FaqI GGGAC 1 cut(s) 633
FatI CATG 6 cut(s) 301, 378, 440, 629, 805, 811
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 2 cut(s) 328, 776
Fnu4HI GCNGC 4 cut(s) 223, 226, 354, 533
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 4 cut(s) 223, 226, 354, 533
FspBI CTAG 1 cut(s) 116
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 1 cut(s) 634
GluI GCNGC 4 cut(s) 223, 226, 354, 533
GsuI CTGGAG 1 cut(s) 748
HaeIII GGCC 3 cut(s) 268, 288, 300
HapII CCGG 2 cut(s) 324, 473
HhaI GCGC 1 cut(s) 635
Hin1II CATG 6 cut(s) 305, 382, 444, 633, 809, 815
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HinfI GANTC 6 cut(s) 33, 199, 383, 588, 603, 638
HpaII CCGG 2 cut(s) 324, 473
HphI GGTGA 1 cut(s) 736
Hpy166II GTNNAC 3 cut(s) 329, 677, 777
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 5 cut(s) 155, 178, 272, 423, 485
Hpy8I GTNNAC 3 cut(s) 329, 677, 777
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 691
HpyCH4IV ACGT 5 cut(s) 207, 318, 331, 597, 673
HpyCH4V TGCA 4 cut(s) 498, 709, 737, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 306, 696
HpyF3I CTNAG 4 cut(s) 9, 255, 270, 570
HpySE526I ACGT 5 cut(s) 207, 318, 331, 597, 673
Hsp92II CATG 6 cut(s) 305, 382, 444, 633, 809, 815
HspAI GCGC 1 cut(s) 633
Kzo9I GATC 6 cut(s) 258, 277, 375, 481, 513, 685
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 212, 340
LweI GCATC 2 cut(s) 141, 241
MaeI CTAG 1 cut(s) 116
MaeII ACGT 5 cut(s) 207, 318, 331, 597, 673
MaeIII GTNAC 1 cut(s) 692
MalI GATC 6 cut(s) 260, 279, 377, 483, 515, 687
MboI GATC 6 cut(s) 258, 277, 375, 481, 513, 685
MboII GAAGA 7 cut(s) 110, 194, 287, 350, 633, 733, 789
MfeI CAATTG 2 cut(s) 387, 704
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 615
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 5 cut(s) 43, 103, 387, 704, 831
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 1 cut(s) 395
MnlI CCTC 6 cut(s) 119, 279, 299, 419, 422, 636
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 5 cut(s) 21, 42, 51, 72, 188
MslI CAYNNNNRTG 1 cut(s) 810
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 2 cut(s) 324, 473
MspR9I CCNGG 3 cut(s) 468, 593, 766
MunI CAATTG 2 cut(s) 387, 704
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 3 cut(s) 468, 593, 766
MwoI GCNNNNNNNGC 3 cut(s) 260, 306, 696
NcoI CCATGG 1 cut(s) 301
NdeII GATC 6 cut(s) 258, 277, 375, 481, 513, 685
NlaIII CATG 6 cut(s) 305, 382, 444, 633, 809, 815
NlaIV GGNNCC 1 cut(s) 653
NmuCI GTSAC 1 cut(s) 692
NsbI TGCGCA 1 cut(s) 634
NspI RCATGY 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 423
PctI GAATGC 1 cut(s) 737
PfeI GAWTC 5 cut(s) 33, 199, 383, 588, 603
PfoI TCCNGGA 1 cut(s) 591
PkrI GCNGC 4 cut(s) 224, 227, 355, 534
Ple19I CGATCG 1 cut(s) 688
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 674
Psp6I CCWGG 3 cut(s) 466, 591, 764
PspGI CCWGG 3 cut(s) 466, 591, 764
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 4 cut(s) 266, 437, 464, 470
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 1 cut(s) 277
PvuI CGATCG 1 cut(s) 688
RsaI GTAC 5 cut(s) 92, 509, 672, 678, 770
RsaNI GTAC 5 cut(s) 91, 508, 671, 677, 769
RseI CAYNNNNRTG 1 cut(s) 810
SaqAI TTAA 5 cut(s) 21, 42, 51, 72, 188
SatI GCNGC 4 cut(s) 223, 226, 354, 533
Sau3AI GATC 6 cut(s) 258, 277, 375, 481, 513, 685
Sau96I GGNCC 4 cut(s) 266, 437, 464, 470
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 3 cut(s) 468, 593, 766
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 2 cut(s) 141, 241
Sfr274I CTCGAG 1 cut(s) 423
SinI GGWCC 3 cut(s) 437, 464, 470
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 2 cut(s) 423, 614
SmoI CTYRAG 2 cut(s) 423, 614
Sse9I AATT 5 cut(s) 43, 103, 387, 704, 831
SsiI CCGC 5 cut(s) 223, 238, 309, 532, 535
SspI AATATT 1 cut(s) 83
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 466, 591, 764
StyI CCWWGG 1 cut(s) 301
TaiI ACGT 5 cut(s) 210, 321, 334, 600, 676
TaqI TCGA 4 cut(s) 135, 424, 486, 684
TaqII GACCGA 1 cut(s) 281
TasI AATT 5 cut(s) 43, 103, 387, 704, 831
TauI GCSGC 2 cut(s) 225, 535
TfiI GAWTC 5 cut(s) 33, 199, 383, 588, 603
Tru1I TTAA 5 cut(s) 21, 42, 51, 72, 188
Tru9I TTAA 5 cut(s) 21, 42, 51, 72, 188
TscAI CASTG 1 cut(s) 701
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 2 cut(s) 225, 353
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 4 cut(s) 351, 443, 595, 822
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 1 cut(s) 701
VpaK11BI GGWCC 3 cut(s) 437, 464, 470
XceI RCATGY 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 2 cut(s) 328, 776
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.