pycom03g03940

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0019555
Physical Location & Seq
Forward (+)
3113868 .. 3117587
3720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGATTCCGGGTTCGGGCATCGAATTAGGCACCCCGTTGATAAAAATGGAGAGCTCGTTCAATCTCGGATTTTGTCAAATCCCAATCGCGCAATCTCCTGTGTGTTTGTAGGTGTTGTCATGGCATCTAAACGCATCTTAAAGGAGCTCAAGGATTTGCAGAAGGACCCGCCTACCTCATGCAGTGCTGGTCCTGTGGCTGAGGACATGTTCCATTGGCAGGCGACAATTATGGGACCTGCTGATAGCCCATACACAGGTGGTGTTTTTCTAGTTTCAATCCATTTCCCTCCGGATTATCCATTCAAGCCTCCGAAGGTTGCATTTAGGACCAAGGTCTTTCACCCAAACATTAATAGCAACGGAAGCATATGTCTTGATATTCTCAAAGAACAATGGAGCCCGGCACTAACCATTTCCAAGGTGCTGCTGTCCATATGCTCTCTGTTGACAGACCCCAACCCAGACGACCCACTTGTCCCTGAAATTGCACACATGTACAAGACGGACAGGGCAAAGTATGAAGCCACTGCTCGCAGTTGGGCGCAGAAGTACGCGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

189

Amino Acids

20.85

Weight (kDa)

8.66

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 45 - 181 2.4e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41700 AT5G41700 AT5G41700 AT5G41700
malus_domestica MD11G1052000.v1.1
pyrus_communis pycom03g03940 pycom11g04240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 247
AccB1I GGYRCC 1 cut(s) 30
AccII CGCG 2 cut(s) 90, 557
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 1 cut(s) 170
AfaI GTAC 2 cut(s) 500, 554
AfiI CCNNNNNNNGG 3 cut(s) 15, 220, 257
AflIII ACRYGT 2 cut(s) 207, 495
AgsI TTSAA 3 cut(s) 62, 279, 307
AloI GAACNNNNNNTCC 3 cut(s) 28, 42, 74
AluBI AGCT 2 cut(s) 55, 148
AluI AGCT 2 cut(s) 55, 148
Alw21I GWGCWC 2 cut(s) 57, 150
Aor13HI TCCGGA 1 cut(s) 292
ApeKI GCWGC 1 cut(s) 427
AseI ATTAAT 1 cut(s) 354
AspLEI GCGC 2 cut(s) 92, 547
AspS9I GGNCC 4 cut(s) 166, 191, 236, 330
AsuC2I CCSGG 2 cut(s) 10, 404
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 4 cut(s) 166, 191, 236, 330
BanI GGYRCC 1 cut(s) 30
BanII GRGCYC 3 cut(s) 57, 150, 404
Bbv12I GWGCWC 2 cut(s) 57, 150
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 414
BccI CCATC 1 cut(s) 553
BcnI CCSGG 2 cut(s) 10, 404
BfaI CTAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 1 cut(s) 428
BlsI GCNGC 1 cut(s) 429
Bme1390I CCNGG 2 cut(s) 10, 404
Bme18I GGWCC 4 cut(s) 166, 191, 236, 330
BmgT120I GGNCC 4 cut(s) 166, 191, 236, 330
BmiI GGNNCC 4 cut(s) 32, 168, 237, 401
BmrFI CCNGG 2 cut(s) 10, 404
BmsI GCATC 3 cut(s) 28, 134, 144
BoxI GACNNNNGTC 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 10, 404
BsaJI CCNNGG 2 cut(s) 333, 420
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 220, 257
BseAI TCCGGA 1 cut(s) 292
BseDI CCNNGG 2 cut(s) 333, 420
BseLI CCNNNNNNNGG 3 cut(s) 15, 220, 257
BseMII CTCAG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 414
Bsh1236I CGCG 2 cut(s) 90, 557
BshNI GGYRCC 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 57, 150
BsiSI CCGG 3 cut(s) 9, 293, 404
BslFI GGGAC 2 cut(s) 249, 464
BslI CCNNNNNNNGG 3 cut(s) 15, 220, 257
BsmFI GGGAC 2 cut(s) 249, 464
Bsp1286I GDGCHC 3 cut(s) 57, 150, 404
Bsp13I TCCGGA 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 498
BspACI CCGC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 193
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 2 cut(s) 90, 557
BspLI GGNNCC 4 cut(s) 32, 168, 237, 401
BspMI ACCTGC 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 30
BsrGI TGTACA 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 333, 420
BssT1I CCWWGG 2 cut(s) 333, 420
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 222, 535
BstDEI CTNAG 1 cut(s) 201
BstFNI CGCG 2 cut(s) 90, 557
BstHHI GCGC 2 cut(s) 92, 547
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstNSI RCATGY 2 cut(s) 211, 499
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 8, 402
BstUI CGCG 2 cut(s) 90, 557
BstV1I GCAGC 1 cut(s) 414
BtsI GCAGTG 2 cut(s) 190, 528
BtsIMutI CAGTG 2 cut(s) 190, 528
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 222, 535
CfoI GCGC 2 cut(s) 92, 547
Cfr13I GGNCC 4 cut(s) 166, 191, 236, 330
Csp6I GTAC 2 cut(s) 499, 553
CviAII CATG 4 cut(s) 121, 180, 208, 496
CviJI RGCY 7 cut(s) 55, 148, 200, 249, 310, 402, 527
CviKI_1 RGCY 7 cut(s) 55, 148, 200, 249, 310, 402, 527
CviQI GTAC 2 cut(s) 499, 553
DdeI CTNAG 1 cut(s) 201
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
Ecl136II GAGCTC 2 cut(s) 55, 148
Eco130I CCWWGG 2 cut(s) 333, 420
Eco24I GRGCYC 3 cut(s) 57, 150, 404
Eco47I GGWCC 4 cut(s) 166, 191, 236, 330
Eco53kI GAGCTC 2 cut(s) 55, 148
EcoICRI GAGCTC 2 cut(s) 55, 148
EcoO109I RGGNCCY 2 cut(s) 166, 236
EcoT14I CCWWGG 2 cut(s) 333, 420
EcoT38I GRGCYC 3 cut(s) 57, 150, 404
ErhI CCWWGG 2 cut(s) 333, 420
FaeI CATG 4 cut(s) 124, 183, 211, 499
FaqI GGGAC 2 cut(s) 249, 464
FatI CATG 4 cut(s) 120, 179, 207, 495
FauI CCCGC 1 cut(s) 177
FauNDI CATATG 2 cut(s) 371, 437
Fnu4HI GCNGC 1 cut(s) 428
FriOI GRGCYC 3 cut(s) 57, 150, 404
Fsp4HI GCNGC 1 cut(s) 428
FspBI CTAG 1 cut(s) 272
GlaI GCGC 2 cut(s) 91, 546
GluI GCNGC 1 cut(s) 428
HapII CCGG 3 cut(s) 9, 293, 404
HhaI GCGC 2 cut(s) 92, 547
Hin1II CATG 4 cut(s) 124, 183, 211, 499
Hin6I GCGC 2 cut(s) 90, 545
HinP1I GCGC 2 cut(s) 90, 545
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 1 cut(s) 5
HpaII CCGG 3 cut(s) 9, 293, 404
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 2 cut(s) 69, 315
Hpy188III TCNNGA 2 cut(s) 293, 377
Hpy8I GTNNAC 1 cut(s) 450
HpyAV CCTTC 2 cut(s) 157, 310
HpyCH4V TGCA 4 cut(s) 160, 183, 323, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpyF3I CTNAG 1 cut(s) 201
Hsp92II CATG 4 cut(s) 124, 183, 211, 499
HspAI GCGC 2 cut(s) 90, 545
Kpn2I TCCGGA 1 cut(s) 292
LmnI GCTCC 2 cut(s) 145, 399
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 3 cut(s) 28, 134, 144
MaeI CTAG 1 cut(s) 272
MhlI GDGCHC 3 cut(s) 57, 150, 404
MluCI AATT 3 cut(s) 24, 228, 486
MnlI CCTC 4 cut(s) 187, 196, 300, 321
MroI TCCGGA 1 cut(s) 292
MseI TTAA 2 cut(s) 140, 354
MspI CCGG 3 cut(s) 9, 293, 404
MspR9I CCNGG 2 cut(s) 10, 404
MvnI CGCG 2 cut(s) 90, 557
MwoI GCNNNNNNNGC 1 cut(s) 366
NciI CCSGG 2 cut(s) 10, 404
NdeI CATATG 2 cut(s) 371, 437
NlaIII CATG 4 cut(s) 124, 183, 211, 499
NlaIV GGNNCC 4 cut(s) 32, 168, 237, 401
NspI RCATGY 2 cut(s) 211, 499
PciI ACATGT 2 cut(s) 207, 495
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 1 cut(s) 429
PpuMI RGGWCCY 2 cut(s) 166, 236
PscI ACATGT 2 cut(s) 207, 495
PshAI GACNNNNGTC 1 cut(s) 335
PshBI ATTAAT 1 cut(s) 354
Psp124BI GAGCTC 2 cut(s) 57, 150
Psp5II RGGWCCY 2 cut(s) 166, 236
PspN4I GGNNCC 4 cut(s) 32, 168, 237, 401
PspPI GGNCC 4 cut(s) 166, 191, 236, 330
PspPPI RGGWCCY 2 cut(s) 166, 236
RsaI GTAC 2 cut(s) 500, 554
RsaNI GTAC 2 cut(s) 499, 553
SacI GAGCTC 2 cut(s) 57, 150
SaqAI TTAA 2 cut(s) 140, 354
SatI GCNGC 1 cut(s) 428
Sau96I GGNCC 4 cut(s) 166, 191, 236, 330
ScrFI CCNGG 2 cut(s) 10, 404
SduI GDGCHC 3 cut(s) 57, 150, 404
SetI ASST 9 cut(s) 57, 115, 150, 179, 241, 262, 321, 339, 426
SfaNI GCATC 3 cut(s) 28, 134, 144
SinI GGWCC 4 cut(s) 166, 191, 236, 330
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 3 cut(s) 24, 228, 486
SsiI CCGC 1 cut(s) 170
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 2 cut(s) 57, 150
StyD4I CCNGG 2 cut(s) 8, 402
StyI CCWWGG 2 cut(s) 333, 420
TaqI TCGA 1 cut(s) 22
TasI AATT 3 cut(s) 24, 228, 486
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 140, 354
Tru9I TTAA 2 cut(s) 140, 354
TscAI CASTG 2 cut(s) 190, 535
TseI GCWGC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 537
TspGWI ACGGA 2 cut(s) 378, 521
TspRI CASTG 2 cut(s) 190, 535
VpaK11BI GGWCC 4 cut(s) 166, 191, 236, 330
VspI ATTAAT 1 cut(s) 354
XceI RCATGY 2 cut(s) 211, 499
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.