pycom03g07570

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0022535
Physical Location & Seq
Reverse (-)
5993641 .. 5996343
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07570.4

Sequence Viewer

Length: 816 bp
ATGAGCAAGATAATATATGTGATTGGCACCTCTGGCTCAGTGGAAGCACAGCAGGTCAATTTTGCTCAAGGAGATGAATATCATGCATTCGGAGGAACTGCAAAGGAGCACTGGGATTCAATGAGGTCCTACTATCAGAAAGCTGCAATGACATATTCAAGAGGAGCACGGGAACATGCAGGTTACCTCGCTGAGCAGCGGTTCAATTCCTCAGGGTTATCACAGGGTGCGGGTCGCATGGTTACGAAGCAATCGGTAGGGGTTTTAAATTCTCTTTGGCATGAATTTTTTGGGGAAATAGTGGAATGCTTGGTAATGCATATTAAACTGATCAAACTGGTGGCTGAGTTAATTTTGAGTTTTGATAACAATTACTGGAGGTTTTATTTTTCTCTTCTTCCCCTGCCATGGGGGTATTTGGCTATAATGAAAGTCATCGAAATTGCTGAGAATGAAGGTATACAATGGAGGGAAGAGAATCGAGGAATGGAGCTTTGGTCTATGCTGGAGTTTATGATGCCAGATCTTTTCACTACTAAGGATGTTGACTTGAAAAATCTATTAATTGGTGAAGATAGAGATTTGATTGGCCGCATGAAATCTATATTGGGACCCTTTATTTTGAGACGCTTAAAATCAGATGTTATGCAACAAGAAGTTCCTAAAATACAGCGGTTGATCACTCGTCGTCGGAGTGTGACCAATGAGCATTCCGTATTTTCAGCAGCTACTGCTTTAGTCGTGAGTGCTTCATTGATTGGGGAGCCTACACCTTTTACTGAGGTTACTCTCACGGCGTCCCAGATTGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

272

Amino Acids

30.83

Weight (kDa)

6.33

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022535)

Species Orthologous Gene IDs
pyrus_communis pycom03g07570 pycom08g07540 pycom15g19660 pycom17g24050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 43, 170
AccB1I GGYRCC 1 cut(s) 26
AccB7I CCANNNNNTGG 1 cut(s) 808
AccI GTMKAC 1 cut(s) 460
AciI CCGC 4 cut(s) 199, 230, 592, 673
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 2 cut(s) 268, 284
AcyI GRCGYC 1 cut(s) 797
AdeI CACNNNGTG 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 408, 409, 808
AgsI TTSAA 4 cut(s) 120, 159, 205, 553
AjuI GAANNNNNNNTTGG 4 cut(s) 478, 510, 590, 622
AluBI AGCT 3 cut(s) 143, 493, 728
AluI AGCT 3 cut(s) 143, 493, 728
Alw21I GWGCWC 2 cut(s) 111, 169
Alw26I GTCTC 1 cut(s) 619
AlwNI CAGNNNCTG 1 cut(s) 731
AoxI GGCC 1 cut(s) 589
ApeKI GCWGC 3 cut(s) 143, 196, 725
ApoI RAATTY 2 cut(s) 268, 284
AseI ATTAAT 1 cut(s) 563
AspS9I GGNCC 2 cut(s) 126, 611
AsuHPI GGTGA 1 cut(s) 581
AvaII GGWCC 2 cut(s) 126, 611
AxyI CCTNAGG 1 cut(s) 211
BanI GGYRCC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 111, 169
BbvI GCAGC 3 cut(s) 130, 208, 737
BceAI ACGGC 1 cut(s) 810
BclI TGATCA 2 cut(s) 330, 678
BcoDI GTCTC 1 cut(s) 619
BfuAI ACCTGC 2 cut(s) 43, 170
BglII AGATCT 1 cut(s) 523
BisI GCNGC 4 cut(s) 144, 197, 592, 726
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 4 cut(s) 145, 198, 593, 727
Bme18I GGWCC 2 cut(s) 126, 611
BmgT120I GGNCC 2 cut(s) 126, 611
BmiI GGNNCC 4 cut(s) 28, 612, 613, 765
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 121
BpmI CTGGAG 2 cut(s) 397, 527
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 797
BsaJI CCNNGG 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 408, 409, 808
Bse1I ACTGG 3 cut(s) 116, 342, 380
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 407
BseGI GGATG 1 cut(s) 547
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 3 cut(s) 408, 409, 808
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 6 cut(s) 51, 183, 225, 336, 438, 771
BseNI ACTGG 3 cut(s) 116, 342, 380
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 130, 208, 737
BshFI GGCC 1 cut(s) 591
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 111, 169
BslFI GGGAC 2 cut(s) 624, 784
BslI CCNNNNNNNGG 3 cut(s) 408, 409, 808
BsmAI GTCTC 1 cut(s) 619
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 2 cut(s) 624, 784
BsmI GAATGC 3 cut(s) 86, 311, 709
BsnI GGCC 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 111, 169
Bsp143I GATC 3 cut(s) 330, 523, 678
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 4 cut(s) 199, 230, 592, 673
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 6 cut(s) 50, 184, 224, 337, 439, 772
BspLI GGNNCC 4 cut(s) 28, 612, 613, 765
BspMI ACCTGC 2 cut(s) 43, 170
BspT107I GGYRCC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 3 cut(s) 116, 342, 380
BssECI CCNNGG 1 cut(s) 407
BssMI GATC 3 cut(s) 330, 523, 678
BssNAI GTATAC 1 cut(s) 461
BssNI GRCGYC 1 cut(s) 797
BssT1I CCWWGG 1 cut(s) 407
Bst1107I GTATAC 1 cut(s) 461
Bst6I CTCTTC 2 cut(s) 399, 468
BstACI GRCGYC 1 cut(s) 797
BstAPI GCANNNNNTGC 1 cut(s) 731
BstDEI CTNAG 7 cut(s) 37, 192, 211, 345, 447, 537, 780
BstDSI CCRYGG 1 cut(s) 407
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 1 cut(s) 547
BstKTI GATC 3 cut(s) 333, 526, 681
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 3 cut(s) 330, 523, 678
BstMWI GCNNNNNNNGC 2 cut(s) 33, 731
BstNSI RCATGY 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 182
BstV1I GCAGC 3 cut(s) 130, 208, 737
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BstZ17I GTATAC 1 cut(s) 461
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 1 cut(s) 547
BtsIMutI CAGTG 2 cut(s) 45, 109
BveI ACCTGC 2 cut(s) 43, 170
CaiI CAGNNNCTG 1 cut(s) 731
Cfr13I GGNCC 2 cut(s) 126, 611
CseI GACGC 2 cut(s) 636, 786
CviAII CATG 6 cut(s) 83, 176, 238, 281, 408, 595
CviJI RGCY 8 cut(s) 36, 143, 344, 422, 493, 591, 728, 766
CviKI_1 RGCY 8 cut(s) 36, 143, 344, 422, 493, 591, 728, 766
DdeI CTNAG 7 cut(s) 37, 192, 211, 345, 447, 537, 780
DpnI GATC 3 cut(s) 332, 525, 680
DpnII GATC 3 cut(s) 330, 523, 678
DraI TTTAAA 1 cut(s) 267
DraIII CACNNNGTG 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 399, 468
EarI CTCTTC 2 cut(s) 399, 468
Eco130I CCWWGG 1 cut(s) 407
Eco47I GGWCC 2 cut(s) 126, 611
Eco81I CCTNAGG 1 cut(s) 211
Eco91I GGTNACC 1 cut(s) 182
EcoO109I RGGNCCY 2 cut(s) 126, 611
EcoO65I GGTNACC 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 407
EcoT22I ATGCAT 2 cut(s) 88, 321
ErhI CCWWGG 1 cut(s) 407
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 6 cut(s) 86, 179, 241, 284, 411, 598
FaqI GGGAC 2 cut(s) 624, 784
FatI CATG 6 cut(s) 82, 175, 237, 280, 407, 594
FauI CCCGC 1 cut(s) 223
FbaI TGATCA 2 cut(s) 330, 678
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 4 cut(s) 144, 197, 592, 726
FokI GGATG 1 cut(s) 554
Fsp4HI GCNGC 4 cut(s) 144, 197, 592, 726
GluI GCNGC 4 cut(s) 144, 197, 592, 726
GsuI CTGGAG 2 cut(s) 397, 527
HaeIII GGCC 1 cut(s) 591
HgaI GACGC 2 cut(s) 636, 786
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 6 cut(s) 86, 179, 241, 284, 411, 598
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 2 cut(s) 116, 478
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 2 cut(s) 461, 547
Hpy188I TCNGA 4 cut(s) 92, 138, 640, 693
Hpy188III TCNNGA 2 cut(s) 159, 742
Hpy8I GTNNAC 2 cut(s) 461, 547
Hpy99I CGWCG 2 cut(s) 690, 693
HpyAV CCTTC 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 86, 101, 146, 179, 319, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 731
HpyF3I CTNAG 7 cut(s) 37, 192, 211, 345, 447, 537, 780
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 6 cut(s) 86, 179, 241, 284, 411, 598
KflI GGGWCCC 1 cut(s) 611
Ksp22I TGATCA 2 cut(s) 330, 678
Kzo9I GATC 3 cut(s) 330, 523, 678
LmnI GCTCC 4 cut(s) 106, 164, 490, 763
Lsp1109I GCAGC 3 cut(s) 130, 208, 737
LweI GCATC 1 cut(s) 507
MaeIII GTNAC 4 cut(s) 182, 241, 697, 784
MalI GATC 3 cut(s) 332, 525, 680
MboI GATC 3 cut(s) 330, 523, 678
MboII GAAGA 4 cut(s) 386, 389, 485, 584
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 111, 169
MluCI AATT 8 cut(s) 58, 205, 268, 284, 351, 370, 441, 564
MmeI TCCRAC 1 cut(s) 671
Mph1103I ATGCAT 2 cut(s) 88, 321
MseI TTAA 5 cut(s) 266, 324, 350, 563, 632
MspA1I CMGCKG 2 cut(s) 199, 673
Mva1269I GAATGC 3 cut(s) 86, 311, 709
MwoI GCNNNNNNNGC 2 cut(s) 33, 731
NcoI CCATGG 1 cut(s) 407
NdeII GATC 3 cut(s) 330, 523, 678
NlaIII CATG 6 cut(s) 86, 179, 241, 284, 411, 598
NlaIV GGNNCC 4 cut(s) 28, 612, 613, 765
NmuCI GTSAC 1 cut(s) 697
NsiI ATGCAT 2 cut(s) 88, 321
NspI RCATGY 1 cut(s) 179
PctI GAATGC 3 cut(s) 86, 311, 709
PfeI GAWTC 2 cut(s) 116, 478
PflMI CCANNNNNTGG 1 cut(s) 808
PkrI GCNGC 4 cut(s) 145, 198, 593, 727
PpuMI RGGWCCY 2 cut(s) 126, 611
PshBI ATTAAT 1 cut(s) 563
Psp5II RGGWCCY 2 cut(s) 126, 611
PspEI GGTNACC 1 cut(s) 182
PspN4I GGNNCC 4 cut(s) 28, 612, 613, 765
PspPI GGNCC 2 cut(s) 126, 611
PspPPI RGGWCCY 2 cut(s) 126, 611
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 523
SaqAI TTAA 5 cut(s) 266, 324, 350, 563, 632
SatI GCNGC 4 cut(s) 144, 197, 592, 726
Sau3AI GATC 3 cut(s) 330, 523, 678
Sau96I GGNCC 2 cut(s) 126, 611
SduI GDGCHC 2 cut(s) 111, 169
SfaNI GCATC 1 cut(s) 507
SinI GGWCC 2 cut(s) 126, 611
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 8 cut(s) 58, 205, 268, 284, 351, 370, 441, 564
SsiI CCGC 4 cut(s) 199, 230, 592, 673
StyI CCWWGG 1 cut(s) 407
TaqI TCGA 2 cut(s) 438, 481
TasI AATT 8 cut(s) 58, 205, 268, 284, 351, 370, 441, 564
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 2 cut(s) 116, 478
Tru1I TTAA 5 cut(s) 266, 324, 350, 563, 632
Tru9I TTAA 5 cut(s) 266, 324, 350, 563, 632
TscAI CASTG 2 cut(s) 45, 116
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 3 cut(s) 143, 196, 725
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 6 cut(s) 90, 297, 443, 468, 611, 741
TspGWI ACGGA 1 cut(s) 703
TspRI CASTG 2 cut(s) 45, 116
Van91I CCANNNNNTGG 1 cut(s) 808
VpaK11BI GGWCC 2 cut(s) 126, 611
VspI ATTAAT 1 cut(s) 563
XapI RAATTY 2 cut(s) 268, 284
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 460
Zsp2I ATGCAT 2 cut(s) 88, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.