pycom08g07540

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0022535
Physical Location & Seq
Reverse (-)
6088458 .. 6091001
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07540.5

Sequence Viewer

Length: 936 bp
ATGTTAACTTTGAGTATACTTACTGTGAGAAGCTTATCAACCTGGTGTTGTTTTCTTTGTTTTAGGAATTATGCGAAGGTACTCACTGATTCTGAAGCCCATTCTCCTGTTAGCCCCAAAAGTAGATCTCTCTCAAAAGTCATTGGCACCTCTGGCTCAGTGGAAGCACAGCAGGTCAATTTTGCTCAAGGAGATGAATATCATGCATTCGGAGGAACTGCAAAGGAGCACTGGGATTCAATGAGGTCCTACTATCAGAAAGCTGCAATGACATATTCAAGAGGAGCACGGGAACATGCAGGTTACCTCGCTGAGCAGCGGTTCAATTCCTCAGGGTTATCACAGGGTGCGGGTCGCATGGTTATGAAGCAATCGGTAGGGGTTTTAAATTCTCTTTGGCATGAATTTTTTGGGGAAATAGTGGAATGCTTGGTAATGCATATTAAACTGATCAAACTGGTGGCTGAGTTAATTTTGAGTTTTGATAACAATTACTGGAGGTTTTATTTTTCTCTTATTCCCCTGCCATGGGGGTATTTGGCTATAATGAAAGTCATCGAAATTGCTGAGAATGAAGGTATACAATGGAGGGAAGAGAATCGAGGAATGGAGCTTTGGTCTATGCTGGAGTTTATGATGCCAGATCTTTTCACTACTAAGGATGTTGACTTGAAAAATCTATTAATTGGTGAAGATAAAGATTTGATTGGCCGCATGAAATCTATATTGGGACCCTTTATTTTGAGACGCTTAAAATCAGATGTTATGCAACAAGAAGTTCCTAAAATACAGCGGTTGATCACTCGTCGTCGGAGTGTGACCAATGAGCATTCCGCATTTTCAGCAGCTACTGCTTTAGTCGTGAGTGCTTCATTGATTGGGGAGCCTACACCTTTTACTGAGGTTACTCTCACGGCGTCCCAGATTGCGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

312

Amino Acids

35.2

Weight (kDa)

7.7

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022535)

Species Orthologous Gene IDs
pyrus_communis pycom03g07570 pycom08g07540 pycom15g19660 pycom17g24050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 163, 290
AccB1I GGYRCC 1 cut(s) 146
AccI GTMKAC 2 cut(s) 16, 580
AciI CCGC 6 cut(s) 319, 350, 712, 793, 834, 929
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 2 cut(s) 388, 404
AcuI CTGAAG 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 917
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 3 cut(s) 528, 529, 928
AgsI TTSAA 4 cut(s) 240, 279, 325, 673
AjnI CCWGG 1 cut(s) 41
AjuI GAANNNNNNNTTGG 4 cut(s) 598, 630, 710, 742
AluBI AGCT 4 cut(s) 33, 263, 613, 848
AluI AGCT 4 cut(s) 33, 263, 613, 848
Alw21I GWGCWC 2 cut(s) 231, 289
Alw26I GTCTC 1 cut(s) 739
AlwNI CAGNNNCTG 1 cut(s) 851
AoxI GGCC 1 cut(s) 709
ApeKI GCWGC 3 cut(s) 263, 316, 845
ApoI RAATTY 2 cut(s) 388, 404
AseI ATTAAT 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 246, 731
AsuHPI GGTGA 1 cut(s) 701
AvaII GGWCC 2 cut(s) 246, 731
AxyI CCTNAGG 1 cut(s) 331
BanI GGYRCC 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 231, 289
BbvI GCAGC 3 cut(s) 250, 328, 857
BceAI ACGGC 1 cut(s) 930
BciT130I CCWGG 1 cut(s) 43
BclI TGATCA 2 cut(s) 450, 798
BcoDI GTCTC 1 cut(s) 739
BfuAI ACCTGC 2 cut(s) 163, 290
BglII AGATCT 2 cut(s) 125, 643
BisI GCNGC 4 cut(s) 264, 317, 712, 846
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 4 cut(s) 265, 318, 713, 847
Bme1390I CCNGG 1 cut(s) 43
Bme18I GGWCC 2 cut(s) 246, 731
BmgT120I GGNCC 2 cut(s) 246, 731
BmiI GGNNCC 4 cut(s) 148, 732, 733, 885
BmrFI CCNGG 1 cut(s) 43
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 627
BmuI ACTGGG 1 cut(s) 241
BpmI CTGGAG 2 cut(s) 517, 647
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 527
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 3 cut(s) 528, 529, 928
Bse1I ACTGG 3 cut(s) 236, 462, 500
Bse21I CCTNAGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 1 cut(s) 667
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 528, 529, 928
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 6 cut(s) 171, 303, 345, 456, 558, 891
BseNI ACTGG 3 cut(s) 236, 462, 500
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 250, 328, 857
BshFI GGCC 1 cut(s) 711
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 2 cut(s) 231, 289
BslFI GGGAC 2 cut(s) 744, 904
BslI CCNNNNNNNGG 3 cut(s) 528, 529, 928
BsmAI GTCTC 1 cut(s) 739
BsmBI CGTCTC 1 cut(s) 739
BsmFI GGGAC 2 cut(s) 744, 904
BsmI GAATGC 3 cut(s) 206, 431, 829
BsnI GGCC 1 cut(s) 711
Bsp1286I GDGCHC 2 cut(s) 231, 289
Bsp143I GATC 4 cut(s) 125, 450, 643, 798
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 527
BspACI CCGC 6 cut(s) 319, 350, 712, 793, 834, 929
BspANI GGCC 1 cut(s) 711
BspCNI CTCAG 6 cut(s) 170, 304, 344, 457, 559, 892
BspLI GGNNCC 4 cut(s) 148, 732, 733, 885
BspMI ACCTGC 2 cut(s) 163, 290
BspT107I GGYRCC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 3 cut(s) 236, 462, 500
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 4 cut(s) 125, 450, 643, 798
BssNAI GTATAC 2 cut(s) 17, 581
BssNI GRCGYC 1 cut(s) 917
BssT1I CCWWGG 1 cut(s) 527
Bst1107I GTATAC 2 cut(s) 17, 581
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 851
BstDEI CTNAG 7 cut(s) 157, 312, 331, 465, 567, 657, 900
BstDSI CCRYGG 1 cut(s) 527
BstEII GGTNACC 1 cut(s) 302
BstF5I GGATG 1 cut(s) 667
BstKTI GATC 4 cut(s) 128, 453, 646, 801
BstMAI GTCTC 1 cut(s) 739
BstMBI GATC 4 cut(s) 125, 450, 643, 798
BstMWI GCNNNNNNNGC 3 cut(s) 153, 842, 851
BstNI CCWGG 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 41
BstV1I GCAGC 3 cut(s) 250, 328, 857
BstX2I RGATCY 2 cut(s) 125, 643
BstYI RGATCY 2 cut(s) 125, 643
BstZ17I GTATAC 2 cut(s) 17, 581
Bsu36I CCTNAGG 1 cut(s) 331
BsuRI GGCC 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 527
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 3 cut(s) 84, 165, 229
BveI ACCTGC 2 cut(s) 163, 290
CaiI CAGNNNCTG 1 cut(s) 851
Cfr13I GGNCC 2 cut(s) 246, 731
CseI GACGC 2 cut(s) 756, 906
CsiI ACCWGGT 1 cut(s) 41
Csp6I GTAC 1 cut(s) 80
CviAII CATG 6 cut(s) 203, 296, 358, 401, 528, 715
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 7 cut(s) 157, 312, 331, 465, 567, 657, 900
DpnI GATC 4 cut(s) 127, 452, 645, 800
DpnII GATC 4 cut(s) 125, 450, 643, 798
DraI TTTAAA 1 cut(s) 387
DraIII CACNNNGTG 1 cut(s) 347
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 527
Eco47I GGWCC 2 cut(s) 246, 731
Eco57I CTGAAG 1 cut(s) 114
Eco81I CCTNAGG 1 cut(s) 331
Eco91I GGTNACC 1 cut(s) 302
EcoO109I RGGNCCY 2 cut(s) 246, 731
EcoO65I GGTNACC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 527
EcoT22I ATGCAT 2 cut(s) 208, 441
ErhI CCWWGG 1 cut(s) 527
Esp3I CGTCTC 1 cut(s) 739
FaeI CATG 6 cut(s) 206, 299, 361, 404, 531, 718
FaqI GGGAC 2 cut(s) 744, 904
FatI CATG 6 cut(s) 202, 295, 357, 400, 527, 714
FauI CCCGC 1 cut(s) 343
FbaI TGATCA 2 cut(s) 450, 798
FblI GTMKAC 2 cut(s) 16, 580
Fnu4HI GCNGC 4 cut(s) 264, 317, 712, 846
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 4 cut(s) 264, 317, 712, 846
GluI GCNGC 4 cut(s) 264, 317, 712, 846
GsuI CTGGAG 2 cut(s) 517, 647
HaeIII GGCC 1 cut(s) 711
HgaI GACGC 2 cut(s) 756, 906
Hin1I GRCGYC 1 cut(s) 917
Hin1II CATG 6 cut(s) 206, 299, 361, 404, 531, 718
HincII GTYRAC 2 cut(s) 6, 667
HindII GTYRAC 2 cut(s) 6, 667
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 3 cut(s) 89, 236, 598
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 4 cut(s) 6, 17, 581, 667
Hpy188I TCNGA 5 cut(s) 94, 212, 258, 760, 813
Hpy188III TCNNGA 2 cut(s) 279, 862
Hpy8I GTNNAC 4 cut(s) 6, 17, 581, 667
Hpy99I CGWCG 2 cut(s) 810, 813
HpyAV CCTTC 2 cut(s) 70, 569
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 6 cut(s) 206, 221, 266, 299, 439, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 842, 851
HpyF3I CTNAG 7 cut(s) 157, 312, 331, 465, 567, 657, 900
Hsp92I GRCGYC 1 cut(s) 917
Hsp92II CATG 6 cut(s) 206, 299, 361, 404, 531, 718
KflI GGGWCCC 1 cut(s) 731
Ksp22I TGATCA 2 cut(s) 450, 798
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 4 cut(s) 125, 450, 643, 798
LmnI GCTCC 4 cut(s) 226, 284, 610, 883
Lsp1109I GCAGC 3 cut(s) 250, 328, 857
LweI GCATC 1 cut(s) 627
MabI ACCWGGT 1 cut(s) 41
MaeIII GTNAC 3 cut(s) 302, 817, 904
MalI GATC 4 cut(s) 127, 452, 645, 800
MboI GATC 4 cut(s) 125, 450, 643, 798
MboII GAAGA 2 cut(s) 605, 704
MflI RGATCY 2 cut(s) 125, 643
MhlI GDGCHC 2 cut(s) 231, 289
MluCI AATT 9 cut(s) 67, 178, 325, 388, 404, 471, 490, 561, 684
MmeI TCCRAC 1 cut(s) 791
Mph1103I ATGCAT 2 cut(s) 208, 441
MseI TTAA 6 cut(s) 5, 386, 444, 470, 683, 752
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 319, 793
MspR9I CCNGG 1 cut(s) 43
Mva1269I GAATGC 3 cut(s) 206, 431, 829
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 153, 842, 851
NcoI CCATGG 1 cut(s) 527
NdeII GATC 4 cut(s) 125, 450, 643, 798
NlaIII CATG 6 cut(s) 206, 299, 361, 404, 531, 718
NlaIV GGNNCC 4 cut(s) 148, 732, 733, 885
NmuCI GTSAC 1 cut(s) 817
NsiI ATGCAT 2 cut(s) 208, 441
NspI RCATGY 1 cut(s) 299
PctI GAATGC 3 cut(s) 206, 431, 829
PfeI GAWTC 3 cut(s) 89, 236, 598
PkrI GCNGC 4 cut(s) 265, 318, 713, 847
PpuMI RGGWCCY 2 cut(s) 246, 731
PshBI ATTAAT 1 cut(s) 683
Psp5II RGGWCCY 2 cut(s) 246, 731
Psp6I CCWGG 1 cut(s) 41
PspEI GGTNACC 1 cut(s) 302
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 4 cut(s) 148, 732, 733, 885
PspPI GGNCC 2 cut(s) 246, 731
PspPPI RGGWCCY 2 cut(s) 246, 731
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 2 cut(s) 125, 643
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 6 cut(s) 5, 386, 444, 470, 683, 752
SatI GCNGC 4 cut(s) 264, 317, 712, 846
Sau3AI GATC 4 cut(s) 125, 450, 643, 798
Sau96I GGNCC 2 cut(s) 246, 731
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 2 cut(s) 231, 289
SexAI ACCWGGT 1 cut(s) 41
SfaNI GCATC 1 cut(s) 627
SinI GGWCC 2 cut(s) 246, 731
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 9 cut(s) 67, 178, 325, 388, 404, 471, 490, 561, 684
SsiI CCGC 6 cut(s) 319, 350, 712, 793, 834, 929
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 527
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 2 cut(s) 558, 601
TasI AATT 9 cut(s) 67, 178, 325, 388, 404, 471, 490, 561, 684
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 3 cut(s) 89, 236, 598
Tru1I TTAA 6 cut(s) 5, 386, 444, 470, 683, 752
Tru9I TTAA 6 cut(s) 5, 386, 444, 470, 683, 752
TscAI CASTG 3 cut(s) 91, 165, 236
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 3 cut(s) 263, 316, 845
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 7 cut(s) 210, 380, 417, 563, 588, 731, 861
TspRI CASTG 3 cut(s) 91, 165, 236
VpaK11BI GGWCC 2 cut(s) 246, 731
VspI ATTAAT 1 cut(s) 683
XapI RAATTY 2 cut(s) 388, 404
XceI RCATGY 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 16, 580
Zsp2I ATGCAT 2 cut(s) 208, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.