pycom03g09360
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0029232
Physical Location & Seq
Forward (+)
7874815 .. 7875330
516 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09360.1

Sequence Viewer

Length: 516 bp
ATGGTCCCCTCCGTGTCATGTTTCCACCCGAAAAGCAAGCGGAAGAAGAAGCAGAGCCATAAAAATGGCCCCGAGTTTGTGTCTGAGCCCGTGGACGTTCGGTCCATGTCGTTTGTCGGGACCCACGAGTACTTGGCCCCGGAGATTGTGTCCGGCGAGGGGCATGGTAGTGCAGTGGACTGGTGGACGTTAGGGATATTTATTTTTGAACTGTTCTATGGTGTGACACCCTTTAGGGGTGTGGACAATGAGCTAACCCTAGCTAACATTGTGGCTCGAGCCCTGGAGCTTCCGAAAGAACCGGCGGTGCCCAGCACGGCCAAGGACCTCATCTCACAGTTGTTGGTCAAGGATCCGGCTCGGCGTTTGGGATCGTTGATGGGTGCATCAGCAGTCAAGAGGCACCCATTTTTCCAAGGGGTTAATTGGGCATTGTTGAGATGTACACCTCCACCATTTATTCCCCCACCATTTAAGAGGCGAGTTGTATCTACTGATCGATCAAAGTTGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

172

Amino Acids

19.06

Weight (kDa)

9.73

Isoelectric Point (pI)

58.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 35 - 138 5e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 43 - 122 2.5e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0029232)

Species Orthologous Gene IDs
pyrus_communis pycom03g09360
rosa_multiflora Rmu_sc0018675.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 307, 402
AciI CCGC 2 cut(s) 40, 305
AclWI GGATC 3 cut(s) 347, 360, 379
AcoI YGGCCR 1 cut(s) 318
AfaI GTAC 3 cut(s) 131, 445, 512
AfiI CCNNNNNNNGG 2 cut(s) 159, 236
AgsI TTSAA 1 cut(s) 209
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 3 cut(s) 253, 263, 289
AluI AGCT 3 cut(s) 253, 263, 289
AlwI GGATC 3 cut(s) 347, 360, 379
Ama87I CYCGRG 2 cut(s) 71, 276
AoxI GGCC 3 cut(s) 67, 135, 318
AspS9I GGNCC 6 cut(s) 4, 68, 102, 120, 136, 325
AsuC2I CCSGG 1 cut(s) 140
AvaI CYCGRG 2 cut(s) 71, 276
AvaII GGWCC 4 cut(s) 4, 102, 120, 325
BaeGI GKGCMC 1 cut(s) 312
BamHI GGATCC 1 cut(s) 352
BanI GGYRCC 2 cut(s) 307, 402
BanII GRGCYC 2 cut(s) 90, 283
BauI CACGAG 1 cut(s) 125
BccI CCATC 1 cut(s) 373
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 284
BcnI CCSGG 1 cut(s) 140
BfaI CTAG 1 cut(s) 260
BmcAI AGTACT 1 cut(s) 131
Bme1390I CCNGG 2 cut(s) 140, 284
Bme18I GGWCC 4 cut(s) 4, 102, 120, 325
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 71, 276
BmgT120I GGNCC 6 cut(s) 4, 68, 102, 120, 136, 325
BmiI GGNNCC 8 cut(s) 6, 70, 121, 122, 138, 309, 354, 404
BmrFI CCNGG 2 cut(s) 140, 284
BmsI GCATC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 499
BsaJI CCNNGG 5 cut(s) 90, 138, 282, 321, 415
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 236
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 185
BseBI CCWGG 1 cut(s) 284
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 5 cut(s) 90, 138, 282, 321, 415
BseLI CCNNNNNNNGG 2 cut(s) 159, 236
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 3 cut(s) 69, 137, 320
BshNI GGYRCC 2 cut(s) 307, 402
BshVI ATCGAT 1 cut(s) 499
BsiHKCI CYCGRG 2 cut(s) 71, 276
BsiSI CCGG 4 cut(s) 140, 153, 302, 356
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 159, 236
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 3 cut(s) 69, 137, 320
BsoBI CYCGRG 2 cut(s) 71, 276
Bsp1286I GDGCHC 3 cut(s) 90, 283, 312
Bsp1407I TGTACA 1 cut(s) 443
Bsp143I GATC 4 cut(s) 352, 371, 496, 500
BspACI CCGC 2 cut(s) 40, 305
BspANI GGCC 3 cut(s) 69, 137, 320
BspCNI CTCAG 1 cut(s) 76
BspDI ATCGAT 1 cut(s) 499
BspLI GGNNCC 8 cut(s) 6, 70, 121, 122, 138, 309, 354, 404
BspPI GGATC 3 cut(s) 347, 360, 379
BspT107I GGYRCC 2 cut(s) 307, 402
BsrFI RCCGGY 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 443
BsrI ACTGG 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 5 cut(s) 90, 138, 282, 321, 415
BssMI GATC 4 cut(s) 352, 371, 496, 500
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 321, 415
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 213, 339
BstAUI TGTACA 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 90
BstKTI GATC 4 cut(s) 355, 374, 499, 503
BstMBI GATC 4 cut(s) 352, 371, 496, 500
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 138, 282
BstSLI GKGCMC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 65
BstYI RGATCY 1 cut(s) 352
Bsu15I ATCGAT 1 cut(s) 499
BsuRI GGCC 3 cut(s) 69, 137, 320
BsuTUI ATCGAT 1 cut(s) 499
BtgI CCRYGG 1 cut(s) 90
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 6 cut(s) 4, 68, 102, 120, 136, 325
ClaI ATCGAT 1 cut(s) 499
Csp6I GTAC 3 cut(s) 130, 444, 511
CviAII CATG 3 cut(s) 18, 106, 164
CviQI GTAC 3 cut(s) 130, 444, 511
DdeI CTNAG 1 cut(s) 84
DpnI GATC 4 cut(s) 354, 373, 498, 502
DpnII GATC 4 cut(s) 352, 371, 496, 500
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 321, 415
Eco24I GRGCYC 2 cut(s) 90, 283
Eco47I GGWCC 4 cut(s) 4, 102, 120, 325
Eco88I CYCGRG 2 cut(s) 71, 276
EcoO109I RGGNCCY 2 cut(s) 120, 325
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 2 cut(s) 321, 415
EcoT38I GRGCYC 2 cut(s) 90, 283
ErhI CCWWGG 2 cut(s) 321, 415
FaeI CATG 3 cut(s) 21, 109, 167
FaiI YATR 5 cut(s) 19, 60, 107, 165, 219
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 17, 105, 163
FriOI GRGCYC 2 cut(s) 90, 283
FspBI CTAG 1 cut(s) 260
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 3 cut(s) 69, 137, 320
HapII CCGG 4 cut(s) 140, 153, 302, 356
Hin1II CATG 3 cut(s) 21, 109, 167
HpaII CCGG 4 cut(s) 140, 153, 302, 356
Hpy166II GTNNAC 5 cut(s) 94, 178, 186, 244, 446
Hpy188I TCNGA 2 cut(s) 85, 294
Hpy188III TCNNGA 2 cut(s) 118, 397
Hpy8I GTNNAC 5 cut(s) 94, 178, 186, 244, 446
HpyCH4III ACNGT 2 cut(s) 213, 339
HpyCH4IV ACGT 2 cut(s) 96, 188
HpyCH4V TGCA 2 cut(s) 173, 386
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 96, 188
Hsp92II CATG 3 cut(s) 21, 109, 167
KflI GGGWCCC 1 cut(s) 120
Kzo9I GATC 4 cut(s) 352, 371, 496, 500
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 8 cut(s) 153, 166, 166, 269, 296, 315, 325, 369
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 260
MaeII ACGT 2 cut(s) 96, 188
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 354, 373, 498, 502
MboI GATC 4 cut(s) 352, 371, 496, 500
MboII GAAGA 2 cut(s) 55, 58
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 90, 283, 312
MluCI AATT 1 cut(s) 424
MnlI CCTC 6 cut(s) 19, 151, 338, 393, 459, 471
MseI TTAA 2 cut(s) 423, 474
MslI CAYNNNNRTG 2 cut(s) 63, 168
MspI CCGG 4 cut(s) 140, 153, 302, 356
MspR9I CCNGG 2 cut(s) 140, 284
MvaI CCWGG 1 cut(s) 284
NciI CCSGG 1 cut(s) 140
NdeII GATC 4 cut(s) 352, 371, 496, 500
NlaIII CATG 3 cut(s) 21, 109, 167
NlaIV GGNNCC 8 cut(s) 6, 70, 121, 122, 138, 309, 354, 404
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PpuMI RGGWCCY 2 cut(s) 120, 325
Psp5II RGGWCCY 2 cut(s) 120, 325
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 8 cut(s) 6, 70, 121, 122, 138, 309, 354, 404
PspPI GGNCC 6 cut(s) 4, 68, 102, 120, 136, 325
PspPPI RGGWCCY 2 cut(s) 120, 325
PspXI VCTCGAGB 1 cut(s) 276
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 3 cut(s) 131, 445, 512
RsaNI GTAC 3 cut(s) 130, 444, 511
RseI CAYNNNNRTG 2 cut(s) 63, 168
SaqAI TTAA 2 cut(s) 423, 474
Sau3AI GATC 4 cut(s) 352, 371, 496, 500
Sau96I GGNCC 6 cut(s) 4, 68, 102, 120, 136, 325
ScaI AGTACT 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 140, 284
SduI GDGCHC 3 cut(s) 90, 283, 312
SetI ASST 7 cut(s) 99, 191, 255, 265, 291, 330, 451
SfaNI GCATC 1 cut(s) 395
Sfr274I CTCGAG 1 cut(s) 276
SinI GGWCC 4 cut(s) 4, 102, 120, 325
SlaI CTCGAG 1 cut(s) 276
SmiMI CAYNNNNRTG 2 cut(s) 63, 168
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
Sse9I AATT 1 cut(s) 424
SsiI CCGC 2 cut(s) 40, 305
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 138, 282
StyI CCWWGG 2 cut(s) 321, 415
TaaI ACNGT 2 cut(s) 213, 339
TaiI ACGT 2 cut(s) 99, 191
TaqI TCGA 2 cut(s) 277, 499
TaqII GACCGA 1 cut(s) 90
TasI AATT 1 cut(s) 424
TatI WGTACW 3 cut(s) 129, 443, 510
Tru1I TTAA 2 cut(s) 423, 474
Tru9I TTAA 2 cut(s) 423, 474
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 4 cut(s) 4, 102, 120, 325
XhoI CTCGAG 1 cut(s) 276
XspI CTAG 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.