pycom03g10800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0029234
Physical Location & Seq
Forward (+)
10151730 .. 10152221
492 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10800.1

Sequence Viewer

Length: 492 bp
ATGACATGTACAGTTGCCTGGATTCAATCTCCCTACGAGACTGGTAAGGGGATTTTCATCATGGGCCGTTTTCTTAATGGCCTTCATCACCCTCGGACAGGCTTGCACACTAAGCTCTTCCTCAAGGAGGGAGGAGTGCACTCCTTTGGATCTGTATGGACTTCTCAGTCACTGGCTTCTTCATCATCCTCAGAAAGATGTACTACATCCAACTTCAGTTTCAGGACTTCTATGGCTTTATTGGGTAGCAGAGCAGCATTAATCGTACCGGTATATCCTACATGTGGATGTAAAGCTAACCTTCCTCATATTAACATCCACAAGCCCAGACTTCCCCATAACTTCTGCTTTCTTAATCAATTTATCATCATACATGTATGCTTCGGGATCAACTACCACGATAATGTCAAAAACCTTGGTAGGACTCGTGGAAGAAGGCTCTTCACTGATTTGAACGTCGAGCTTATACATGAAAGTGGCAGTGGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

164

Amino Acids

18.17

Weight (kDa)

9.69

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029234)

Species Orthologous Gene IDs
pyrus_communis pycom03g10800 pycom10g10080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AclWI GGATC 2 cut(s) 157, 395
AcoI YGGCCR 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 199
AfaI GTAC 3 cut(s) 10, 202, 267
AfiI CCNNNNNNNGG 3 cut(s) 98, 127, 284
AflIII ACRYGT 3 cut(s) 5, 281, 373
AgeI ACCGGT 1 cut(s) 268
AgsI TTSAA 2 cut(s) 26, 454
AjnI CCWGG 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 3 cut(s) 115, 296, 463
AluI AGCT 3 cut(s) 115, 296, 463
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 32
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 2 cut(s) 157, 395
AlwNI CAGNNNCTG 1 cut(s) 172
AoxI GGCC 3 cut(s) 64, 79, 484
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 254
AseI ATTAAT 1 cut(s) 260
AsiGI ACCGGT 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 486
BauI CACGAG 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 266
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 32
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 92, 415
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 127, 284
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 46, 177
Bse8I GATNNNNATC 1 cut(s) 56
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 92, 415
BseGI GGATG 4 cut(s) 185, 206, 293, 315
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 3 cut(s) 98, 127, 284
BseMII CTCAG 2 cut(s) 179, 204
BseNI ACTGG 2 cut(s) 46, 177
BseRI GAGGAG 1 cut(s) 147
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 1 cut(s) 266
BshFI GGCC 3 cut(s) 66, 81, 486
BshTI ACCGGT 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 98, 127, 284
BsmAI GTCTC 1 cut(s) 32
BsnI GGCC 3 cut(s) 66, 81, 486
Bsp1286I GDGCHC 1 cut(s) 141
Bsp1407I TGTACA 1 cut(s) 8
Bsp143I GATC 2 cut(s) 149, 387
BspANI GGCC 3 cut(s) 66, 81, 486
BspCNI CTCAG 2 cut(s) 178, 203
BspPI GGATC 2 cut(s) 157, 395
BspQI GCTCTTC 2 cut(s) 122, 446
BsrFI RCCGGY 1 cut(s) 268
BsrGI TGTACA 1 cut(s) 8
BsrI ACTGG 2 cut(s) 46, 177
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 92, 415
BssMI GATC 2 cut(s) 149, 387
BssSI CACGAG 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 415
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 2 cut(s) 122, 446
BstAUI TGTACA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 3 cut(s) 111, 165, 190
BstENI CCTNNNNNAGG 2 cut(s) 96, 125
BstF5I GGATG 4 cut(s) 185, 206, 293, 315
BstKTI GATC 2 cut(s) 152, 390
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 149, 387
BstMWI GCNNNNNNNGC 1 cut(s) 112
BstNI CCWGG 1 cut(s) 19
BstNSI RCATGY 3 cut(s) 9, 285, 377
BstSCI CCNGG 1 cut(s) 17
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 3 cut(s) 66, 81, 486
BtsCI GGATG 4 cut(s) 185, 206, 293, 315
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 3 cut(s) 170, 444, 487
Cac8I GCNNGC 1 cut(s) 104
CaiI CAGNNNCTG 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 3 cut(s) 9, 201, 266
CspAI ACCGGT 1 cut(s) 268
CviAII CATG 5 cut(s) 6, 61, 282, 374, 470
CviQI GTAC 3 cut(s) 9, 201, 266
DdeI CTNAG 3 cut(s) 111, 165, 190
DpnI GATC 2 cut(s) 151, 389
DpnII GATC 2 cut(s) 149, 387
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 122, 446
EarI CTCTTC 2 cut(s) 122, 446
Eco130I CCWWGG 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 199
EcoNI CCTNNNNNAGG 2 cut(s) 96, 125
EcoRII CCWGG 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 5 cut(s) 9, 64, 285, 377, 473
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FatI CATG 5 cut(s) 5, 60, 281, 373, 469
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 4 cut(s) 172, 193, 300, 302
Fsp4HI GCNGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 3 cut(s) 66, 81, 486
HapII CCGG 1 cut(s) 269
Hin1II CATG 5 cut(s) 9, 64, 285, 377, 473
HinfI GANTC 2 cut(s) 22, 424
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 2 cut(s) 96, 193
Hpy188III TCNNGA 2 cut(s) 223, 385
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 3 cut(s) 92, 311, 429
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 106, 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 112
HpyF3I CTNAG 3 cut(s) 111, 165, 190
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 5 cut(s) 9, 64, 285, 377, 473
Kzo9I GATC 2 cut(s) 149, 387
LguI GCTCTTC 2 cut(s) 122, 446
LpnPI CCDG 8 cut(s) 4, 27, 31, 84, 158, 208, 282, 340
Lsp1109I GCAGC 1 cut(s) 266
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 168
MalI GATC 2 cut(s) 151, 389
MboI GATC 2 cut(s) 149, 387
MboII GAAGA 4 cut(s) 109, 171, 433, 444
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 1 cut(s) 359
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 6 cut(s) 102, 121, 125, 131, 199, 315
Mox20I TGGCCA 1 cut(s) 486
MscI TGGCCA 1 cut(s) 486
MseI TTAA 4 cut(s) 75, 260, 312, 354
MslI CAYNNNNRTG 3 cut(s) 286, 402, 474
Msp20I TGGCCA 1 cut(s) 486
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 19
MvaI CCWGG 1 cut(s) 19
MwoI GCNNNNNNNGC 1 cut(s) 112
NdeII GATC 2 cut(s) 149, 387
NlaIII CATG 5 cut(s) 9, 64, 285, 377, 473
NmuCI GTSAC 1 cut(s) 168
NspI RCATGY 3 cut(s) 9, 285, 377
PciI ACATGT 3 cut(s) 5, 281, 373
PciSI GCTCTTC 2 cut(s) 122, 446
PfeI GAWTC 1 cut(s) 22
PinAI ACCGGT 1 cut(s) 268
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PscI ACATGT 3 cut(s) 5, 281, 373
PshBI ATTAAT 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 17
PspGI CCWGG 1 cut(s) 17
PspPI GGNCC 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 3 cut(s) 10, 202, 267
RsaNI GTAC 3 cut(s) 9, 201, 266
RseI CAYNNNNRTG 3 cut(s) 286, 402, 474
SapI GCTCTTC 2 cut(s) 122, 446
SaqAI TTAA 4 cut(s) 75, 260, 312, 354
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 149, 387
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 1 cut(s) 19
SduI GDGCHC 1 cut(s) 141
SetI ASST 6 cut(s) 117, 298, 303, 417, 459, 465
SmiMI CAYNNNNRTG 3 cut(s) 286, 402, 474
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 17
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 459
TaqI TCGA 1 cut(s) 459
TasI AATT 1 cut(s) 359
TatI WGTACW 2 cut(s) 8, 200
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 4 cut(s) 75, 260, 312, 354
Tru9I TTAA 4 cut(s) 75, 260, 312, 354
TscAI CASTG 3 cut(s) 177, 451, 487
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 4 cut(s) 46, 74, 171, 486
TspRI CASTG 3 cut(s) 177, 451, 487
VneI GTGCAC 1 cut(s) 137
VspI ATTAAT 1 cut(s) 260
XagI CCTNNNNNAGG 2 cut(s) 96, 125
XceI RCATGY 3 cut(s) 9, 285, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.