pycom03g16330

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0029241
Physical Location & Seq
Forward (+)
17487586 .. 17489009
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16330.4

Sequence Viewer

Length: 1215 bp
ATGGATAGTTTCGAAAAATTGATTGAAAAGGCTAGAGGAGCTGTGTCTATCGGTGGAGAACCAATCCAGCGACTTGGACAGGTGCCAATATTTACCACGCCCTTAGGTGTAGAGAGCCTGAAAGCAACGACTTGTTGTCCTACATGCAGATTCTGTATGAGATCTACTTCCAGATTGCTCAGGGAACCCAGTGGGTTACTCTCCTTCAAGCACTTGCAGCAAGACCCAGTGGGGCTCCCCATGTACGAATTACAGGTATCGATCGATCCTGTTTCTAAGCATGCCCGTGGTGATGGGTTGGAGGCAGTAGGGAGACGGTTGAAAGCCATCTCAGACAAATTTAACATCCCAGTTGAATTTCATGGAGTCCCAGTTTTCGCACCTGATGTCACACGGGACATGCTTGATGTCAGGCCGGGAGAGGCTCTTGCTGTGAACTTCCCTCTGCAGCTTCATCACACACCAGATGAAAGCGTTGATGAGAACAATCCGAGGGATGGGCTGCTGAGAACAGTGAGGTCTCTAAATCCAAAGGTAACCACTTTGGTGGAACAAGAATCAAACACGAACACAACCCCCTTTTTCAATAGGTTCGTGGAAACTCTTGAGTACTACTTGGCGATGTTCGAGTCTATTGACGTGACCCTGCCAAGGAACAGCAAGGAAAGAATCAATGTGGAGCAGCATTGCTTGGCAAGAGATATTGTGAATGTCATTGCTTGCGAGGGGAAGGAAAGGGTGGAACGTCACGAGCTCTTTGGCAAGTGGAAATCCAGGTTGACAATGGCAGGGTTTCAGCAGTCCCCGTTGAGCTCATATGTAAACTCTGTCATTGGGAGTCTGCTGAGGTGTTACTCGGAGCATTATACACTGGTTGAGAAGGACGGTGCTATGCTCTTGGGATGGAAGGACAGGAACTTAATATCAGCTTCTTATAATTTACCAGTTTGTATTTTTCTTTTAATGCAATGCACAAAGCCCTATAGTTTCTTTCTACGACCCTGTTCACTTTTGTACTTAGGATGGCCGGCATTTGTATGCCGCTGCAGAATGTGGTTCCCTACCACCGAACGTGTTACCGCTGTGTATACAAGATTCTGGTTTGTTTTACTTTTACAAAGTCTTTTACTAATAACTAAGACTAATCCGGCTTCGCTTCTTCATTCCCGCCCCTCCTCAATCTCTTTTCACCCGAGTATTTCCATGAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

405

Amino Acids

45.85

Weight (kDa)

8.67

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029241)

Species Orthologous Gene IDs
pyrus_communis pycom03g16330
rosa_multiflora Rmu_co8108556.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 1088
AciI CCGC 3 cut(s) 1042, 1080, 1168
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 1025
AcsI RAATTY 2 cut(s) 338, 356
AfaI GTAC 3 cut(s) 245, 611, 1016
AfiI CCNNNNNNNGG 2 cut(s) 497, 651
AflIII ACRYGT 1 cut(s) 1072
AgsI TTSAA 5 cut(s) 26, 208, 322, 356, 586
AhdI GACNNNNNGTC 1 cut(s) 135
AjiI CACGTC 1 cut(s) 640
AjnI CCWGG 1 cut(s) 773
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 5 cut(s) 41, 451, 754, 813, 929
AluI AGCT 5 cut(s) 41, 451, 754, 813, 929
Alw21I GWGCWC 2 cut(s) 756, 815
Alw26I GTCTC 2 cut(s) 307, 525
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 153
Ama87I CYCGRG 1 cut(s) 1192
AoxI GGCC 2 cut(s) 413, 1025
ApeKI GCWGC 5 cut(s) 217, 448, 502, 682, 1044
ApoI RAATTY 2 cut(s) 338, 356
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 302, 1181
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 1192
AxyI CCTNAGG 1 cut(s) 103
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 3 cut(s) 237, 756, 815
BauI CACGAG 1 cut(s) 749
Bbv12I GWGCWC 2 cut(s) 756, 815
BbvCI CCTCAGC 1 cut(s) 845
BbvI GCAGC 5 cut(s) 229, 460, 489, 694, 1031
BccI CCATC 5 cut(s) 287, 335, 491, 897, 1017
BciT130I CCWGG 1 cut(s) 775
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 2 cut(s) 307, 525
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 3 cut(s) 446, 982, 1045
BglII AGATCT 1 cut(s) 161
BisI GCNGC 6 cut(s) 218, 449, 503, 683, 1042, 1045
BlsI GCNGC 6 cut(s) 219, 450, 504, 684, 1043, 1046
BmcAI AGTACT 1 cut(s) 611
Bme1390I CCNGG 2 cut(s) 417, 775
BmeRI GACNNNNNGTC 1 cut(s) 135
BmeT110I CYCGRG 1 cut(s) 1192
BmgBI CACGTC 1 cut(s) 640
BmiI GGNNCC 4 cut(s) 84, 186, 236, 1058
BmrFI CCNGG 2 cut(s) 417, 775
BmrI ACTGGG 4 cut(s) 183, 221, 344, 365
BmuI ACTGGG 4 cut(s) 183, 221, 344, 365
Bpu10I CCTNAGC 2 cut(s) 179, 845
Bpu14I TTCGAA 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 626
BpuMI CCSGG 1 cut(s) 417
Bsa29I ATCGAT 2 cut(s) 260, 264
BsaI GGTCTC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 286, 491, 650
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 651
Bse118I RCCGGY 1 cut(s) 1027
Bse1I ACTGG 6 cut(s) 189, 227, 350, 371, 876, 944
Bse21I CCTNAGG 1 cut(s) 103
Bse3DI GCAATG 3 cut(s) 685, 714, 974
BseBI CCWGG 1 cut(s) 775
BseCI ATCGAT 2 cut(s) 260, 264
BseDI CCNNGG 3 cut(s) 286, 491, 650
BseGI GGATG 4 cut(s) 345, 502, 908, 1028
BseLI CCNNNNNNNGG 2 cut(s) 497, 651
BseMI GCAATG 3 cut(s) 685, 714, 974
BseMII CTCAG 4 cut(s) 193, 345, 497, 836
BseNI ACTGG 6 cut(s) 189, 227, 350, 371, 876, 944
BseRI GAGGAG 2 cut(s) 51, 1165
BseXI GCAGC 5 cut(s) 229, 460, 489, 694, 1031
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 2 cut(s) 415, 1027
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 2 cut(s) 260, 264
BsiEI CGRYCG 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 756, 815
BsiHKCI CYCGRG 1 cut(s) 1192
BsiSI CCGG 3 cut(s) 416, 1028, 1148
BslFI GGGAC 3 cut(s) 353, 410, 787
BslI CCNNNNNNNGG 2 cut(s) 497, 651
BsmAI GTCTC 2 cut(s) 307, 525
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 3 cut(s) 353, 410, 787
BsnI GGCC 2 cut(s) 415, 1027
Bso31I GGTCTC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 1192
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 237, 756, 815
Bsp143I GATC 3 cut(s) 161, 261, 265
BspACI CCGC 3 cut(s) 1042, 1080, 1168
BspANI GGCC 2 cut(s) 415, 1027
BspCNI CTCAG 4 cut(s) 192, 344, 498, 837
BspDI ATCGAT 2 cut(s) 260, 264
BspLI GGNNCC 4 cut(s) 84, 186, 236, 1058
BspMAI CTGCAG 2 cut(s) 450, 1049
BspPI GGATC 1 cut(s) 260
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 82
BspTNI GGTCTC 1 cut(s) 525
BsrDI GCAATG 3 cut(s) 685, 714, 974
BsrFI RCCGGY 1 cut(s) 1027
BsrI ACTGG 6 cut(s) 189, 227, 350, 371, 876, 944
BssAI RCCGGY 1 cut(s) 1027
BssECI CCNNGG 3 cut(s) 286, 491, 650
BssMI GATC 3 cut(s) 161, 261, 265
BssNAI GTATAC 1 cut(s) 1089
BssSI CACGAG 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 650
Bst1107I GTATAC 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 775
Bst4CI ACNGT 3 cut(s) 318, 514, 887
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 282, 721, 1029
BstDEI CTNAG 8 cut(s) 103, 179, 276, 331, 506, 845, 1018, 1137
BstDSI CCRYGG 1 cut(s) 286
BstEII GGTNACC 1 cut(s) 535
BstF5I GGATG 4 cut(s) 345, 502, 908, 1028
BstKTI GATC 3 cut(s) 164, 264, 268
BstMAI GTCTC 2 cut(s) 307, 525
BstMBI GATC 3 cut(s) 161, 261, 265
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 2 cut(s) 38, 217
BstNI CCWGG 1 cut(s) 775
BstNSI RCATGY 3 cut(s) 147, 284, 403
BstPI GGTNACC 1 cut(s) 535
BstSCI CCNGG 2 cut(s) 415, 773
BstSFI CTRYAG 3 cut(s) 446, 982, 1045
BstV1I GCAGC 5 cut(s) 229, 460, 489, 694, 1031
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 2 cut(s) 74, 547
BstYI RGATCY 1 cut(s) 161
BstZ17I GTATAC 1 cut(s) 1089
Bsu15I ATCGAT 2 cut(s) 260, 264
Bsu36I CCTNAGG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 415, 1027
BsuTUI ATCGAT 2 cut(s) 260, 264
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 635
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 4 cut(s) 345, 502, 908, 1028
BtsIMutI CAGTG 4 cut(s) 196, 234, 519, 869
Cac8I GCNNGC 3 cut(s) 282, 721, 1029
CaiI CAGNNNCTG 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 1027
ClaI ATCGAT 2 cut(s) 260, 264
Csp6I GTAC 3 cut(s) 244, 610, 1015
CviAII CATG 6 cut(s) 144, 241, 281, 362, 400, 1204
CviQI GTAC 3 cut(s) 244, 610, 1015
DdeI CTNAG 8 cut(s) 103, 179, 276, 331, 506, 845, 1018, 1137
DpnI GATC 3 cut(s) 163, 263, 267
DpnII GATC 3 cut(s) 161, 261, 265
DriI GACNNNNNGTC 1 cut(s) 135
EaeI YGGCCR 1 cut(s) 1025
Eam1105I GACNNNNNGTC 1 cut(s) 135
Ecl136II GAGCTC 2 cut(s) 754, 813
Eco130I CCWWGG 1 cut(s) 650
Eco24I GRGCYC 3 cut(s) 237, 756, 815
Eco31I GGTCTC 1 cut(s) 525
Eco53kI GAGCTC 2 cut(s) 754, 813
Eco81I CCTNAGG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 1192
Eco91I GGTNACC 1 cut(s) 535
EcoICRI GAGCTC 2 cut(s) 754, 813
EcoO65I GGTNACC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 773
EcoT14I CCWWGG 1 cut(s) 650
EcoT38I GRGCYC 3 cut(s) 237, 756, 815
ErhI CCWWGG 1 cut(s) 650
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 6 cut(s) 147, 244, 284, 365, 403, 1207
FaqI GGGAC 3 cut(s) 353, 410, 787
FatI CATG 6 cut(s) 143, 240, 280, 361, 399, 1203
FauI CCCGC 1 cut(s) 1175
FauNDI CATATG 1 cut(s) 817
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 6 cut(s) 218, 449, 503, 683, 1042, 1045
FokI GGATG 4 cut(s) 332, 509, 915, 1035
FriOI GRGCYC 3 cut(s) 237, 756, 815
Fsp4HI GCNGC 6 cut(s) 218, 449, 503, 683, 1042, 1045
FspBI CTAG 1 cut(s) 33
GluI GCNGC 6 cut(s) 218, 449, 503, 683, 1042, 1045
HaeIII GGCC 2 cut(s) 415, 1027
HapII CCGG 3 cut(s) 416, 1028, 1148
Hin1II CATG 6 cut(s) 147, 244, 284, 365, 403, 1207
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HinfI GANTC 7 cut(s) 150, 366, 557, 629, 669, 838, 1095
HpaII CCGG 3 cut(s) 416, 1028, 1148
HphI GGTGA 2 cut(s) 302, 1181
Hpy166II GTNNAC 5 cut(s) 436, 780, 823, 1007, 1089
Hpy188I TCNGA 3 cut(s) 334, 492, 859
Hpy188III TCNNGA 3 cut(s) 171, 605, 749
Hpy8I GTNNAC 5 cut(s) 436, 780, 823, 1007, 1089
HpyAV CCTTC 4 cut(s) 214, 724, 874, 901
HpyCH4III ACNGT 3 cut(s) 318, 514, 887
HpyCH4IV ACGT 3 cut(s) 639, 745, 1072
HpyCH4V TGCA 6 cut(s) 147, 217, 448, 967, 972, 1047
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 217
HpyF3I CTNAG 8 cut(s) 103, 179, 276, 331, 506, 845, 1018, 1137
HpySE526I ACGT 3 cut(s) 639, 745, 1072
Hsp92II CATG 6 cut(s) 147, 244, 284, 365, 403, 1207
KroI GCCGGC 1 cut(s) 1027
KroNI GCCGGC 1 cut(s) 1029
Kzo9I GATC 3 cut(s) 161, 261, 265
LmnI GCTCC 4 cut(s) 38, 240, 679, 859
Lsp1109I GCAGC 5 cut(s) 229, 460, 489, 694, 1031
MaeI CTAG 1 cut(s) 33
MaeII ACGT 3 cut(s) 639, 745, 1072
MaeIII GTNAC 7 cut(s) 195, 388, 535, 640, 746, 851, 1075
MalI GATC 3 cut(s) 163, 263, 267
MboI GATC 3 cut(s) 161, 261, 265
MboII GAAGA 1 cut(s) 1151
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 3 cut(s) 237, 756, 815
MluCI AATT 5 cut(s) 17, 248, 338, 356, 937
MlyI GAGTC 3 cut(s) 375, 638, 847
MmeI TCCRAC 1 cut(s) 279
MroNI GCCGGC 1 cut(s) 1027
MseI TTAA 3 cut(s) 342, 920, 962
MslI CAYNNNNRTG 3 cut(s) 285, 545, 1036
MspA1I CMGCKG 2 cut(s) 1044, 1082
MspI CCGG 3 cut(s) 416, 1028, 1148
MspR9I CCNGG 2 cut(s) 417, 775
MvaI CCWGG 1 cut(s) 775
MwoI GCNNNNNNNGC 2 cut(s) 38, 217
NaeI GCCGGC 1 cut(s) 1029
NciI CCSGG 1 cut(s) 417
NdeI CATATG 1 cut(s) 817
NdeII GATC 3 cut(s) 161, 261, 265
NgoMIV GCCGGC 1 cut(s) 1027
NlaIII CATG 6 cut(s) 147, 244, 284, 365, 403, 1207
NlaIV GGNNCC 4 cut(s) 84, 186, 236, 1058
NmuCI GTSAC 3 cut(s) 388, 640, 746
NspI RCATGY 3 cut(s) 147, 284, 403
NspV TTCGAA 1 cut(s) 12
OliI CACNNNNGTG 1 cut(s) 545
PaeI GCATGC 1 cut(s) 284
PdiI GCCGGC 1 cut(s) 1029
PfeI GAWTC 4 cut(s) 150, 557, 669, 1095
PkrI GCNGC 6 cut(s) 219, 450, 504, 684, 1043, 1046
Ple19I CGATCG 1 cut(s) 264
PleI GAGTC 3 cut(s) 374, 637, 846
PpsI GAGTC 3 cut(s) 374, 637, 846
PsiI TTATAA 1 cut(s) 936
Psp124BI GAGCTC 2 cut(s) 756, 815
Psp6I CCWGG 1 cut(s) 773
PspEI GGTNACC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 773
PspN4I GGNNCC 4 cut(s) 84, 186, 236, 1058
PstI CTGCAG 2 cut(s) 450, 1049
PstNI CAGNNNCTG 1 cut(s) 153
PsuI RGATCY 1 cut(s) 161
PvuI CGATCG 1 cut(s) 264
RsaI GTAC 3 cut(s) 245, 611, 1016
RsaNI GTAC 3 cut(s) 244, 610, 1015
RseI CAYNNNNRTG 3 cut(s) 285, 545, 1036
SacI GAGCTC 2 cut(s) 756, 815
SaqAI TTAA 3 cut(s) 342, 920, 962
SatI GCNGC 6 cut(s) 218, 449, 503, 683, 1042, 1045
Sau3AI GATC 3 cut(s) 161, 261, 265
ScaI AGTACT 1 cut(s) 611
SchI GAGTC 3 cut(s) 375, 638, 847
ScrFI CCNGG 2 cut(s) 417, 775
SduI GDGCHC 3 cut(s) 237, 756, 815
SfcI CTRYAG 3 cut(s) 446, 982, 1045
SfuI TTCGAA 1 cut(s) 12
SmiMI CAYNNNNRTG 3 cut(s) 285, 545, 1036
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SphI GCATGC 1 cut(s) 284
Sse9I AATT 5 cut(s) 17, 248, 338, 356, 937
SsiI CCGC 3 cut(s) 1042, 1080, 1168
SspI AATATT 1 cut(s) 90
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 2 cut(s) 756, 815
StyD4I CCNGG 2 cut(s) 415, 773
StyI CCWWGG 1 cut(s) 650
TaaI ACNGT 3 cut(s) 318, 514, 887
TaiI ACGT 3 cut(s) 642, 748, 1075
TaqI TCGA 4 cut(s) 12, 260, 264, 627
TasI AATT 5 cut(s) 17, 248, 338, 356, 937
TatI WGTACW 2 cut(s) 609, 1014
TauI GCSGC 1 cut(s) 1044
TfiI GAWTC 4 cut(s) 150, 557, 669, 1095
Tru1I TTAA 3 cut(s) 342, 920, 962
Tru9I TTAA 3 cut(s) 342, 920, 962
TscAI CASTG 4 cut(s) 196, 234, 519, 876
TseFI GTSAC 3 cut(s) 388, 640, 746
TseI GCWGC 5 cut(s) 217, 448, 502, 682, 1044
Tsp45I GTSAC 3 cut(s) 388, 640, 746
TspDTI ATGAA 4 cut(s) 350, 443, 483, 1151
TspRI CASTG 4 cut(s) 196, 234, 519, 876
XapI RAATTY 2 cut(s) 338, 356
XceI RCATGY 3 cut(s) 147, 284, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmiI GTMKAC 1 cut(s) 1088
XspI CTAG 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.