Rmu_co8108556.1_g000001

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8108556.1
OG0029241
Physical Location & Seq
Forward (+)
1 .. 594
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8108556.1_g000001.1.cds

Sequence Viewer

Length: 594 bp
gttgaatttcacggggttccagtttttgcacctgatgtcacaccggacatgcttgatgtcaggcctggtgaggctctagcagtgaactttcccctgcagcttcaccacacaccagatgaaagcgttgatgtgaacaatccaagggatggacttctgaggatagtcaagtcactatctccaaaggtgactactttggttgagcaagaatcaaacacgaatacaacccctttcttgaatcggtttatagagactctagactactatttggcaatgtttgagtctattgacgtgactctgccgaggaacagcaaggagaggatcaacgtggagcagcattgtctggctcgagatattgtaaatgtgattgcctgcgaaggaaaggaaagggtggaacgccacgagctctttgggaagtggaagtccaggttgacaatggcaggatttcggcaatacccattaagctcatatgtcaactctgtcataaggagtctactgaggtgttactcagagcattataagctggaggagaaggatggtgctatgctgttgggatggaaggagaggaacttggtatcagcttctgcgtggcactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

197

Amino Acids

22.61

Weight (kDa)

5.99

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029241)

Species Orthologous Gene IDs
pyrus_communis pycom03g16330
rosa_multiflora Rmu_co8108556.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AccB7I CCANNNNNTGG 1 cut(s) 146
AccI GTMKAC 1 cut(s) 490
AclWI GGATC 1 cut(s) 326
AcsI RAATTY 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 2 cut(s) 5, 235
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 64, 422
AluBI AGCT 5 cut(s) 100, 403, 462, 520, 578
AluI AGCT 5 cut(s) 100, 403, 462, 520, 578
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 581
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 97, 331
ApoI RAATTY 1 cut(s) 5
AsuHPI GGTGA 3 cut(s) 80, 95, 196
AvaI CYCGRG 1 cut(s) 345
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 109, 343
BccI CCATC 3 cut(s) 140, 527, 546
BciT130I CCWGG 2 cut(s) 66, 424
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 77, 254
BfmI CTRYAG 1 cut(s) 95
BisI GCNGC 2 cut(s) 98, 332
BlsI GCNGC 2 cut(s) 99, 333
Bme1390I CCNGG 2 cut(s) 66, 424
BmeT110I CYCGRG 1 cut(s) 345
BmgBI CACGTC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 2 cut(s) 66, 424
BpmI CTGGAG 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 140, 299
BsaWI WCCGGW 1 cut(s) 43
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 2 cut(s) 66, 424
BseDI CCNNGG 2 cut(s) 140, 299
BseGI GGATG 3 cut(s) 151, 538, 557
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 146, 485, 519
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 2 cut(s) 109, 343
BshFI GGCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 1 cut(s) 318
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 3 cut(s) 147, 486, 518
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 99
BspPI GGATC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 140, 299
BssMI GATC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 66, 424
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 3 cut(s) 155, 494, 505
BstF5I GGATG 3 cut(s) 151, 538, 557
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 2 cut(s) 66, 424
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 64, 422
BstSFI CTRYAG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 109, 343
BsuRI GGCC 1 cut(s) 64
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 3 cut(s) 151, 538, 557
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 589
Cac8I GCNNGC 1 cut(s) 370
CaiI CAGNNNCTG 1 cut(s) 581
CviAII CATG 1 cut(s) 49
CviJI RGCY 8 cut(s) 64, 74, 100, 344, 403, 462, 520, 578
CviKI_1 RGCY 8 cut(s) 64, 74, 100, 344, 403, 462, 520, 578
DdeI CTNAG 3 cut(s) 155, 494, 505
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 140
Eco147I AGGCCT 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 345
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 64, 422
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 1 cut(s) 52
FaiI YATR 7 cut(s) 50, 245, 466, 468, 482, 516, 542
FatI CATG 1 cut(s) 48
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 98, 332
FokI GGATG 3 cut(s) 158, 545, 564
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 2 cut(s) 98, 332
FspBI CTAG 2 cut(s) 77, 254
GluI GCNGC 2 cut(s) 98, 332
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 44
Hin1II CATG 1 cut(s) 52
HincII GTYRAC 2 cut(s) 429, 472
HindII GTYRAC 2 cut(s) 429, 472
HinfI GANTC 6 cut(s) 206, 235, 250, 278, 292, 487
HpaII CCGG 1 cut(s) 44
HphI GGTGA 3 cut(s) 80, 95, 196
Hpy166II GTNNAC 5 cut(s) 85, 133, 429, 472, 491
Hpy188I TCNGA 2 cut(s) 156, 508
Hpy188III TCNNGA 3 cut(s) 232, 254, 347
Hpy8I GTNNAC 5 cut(s) 85, 133, 429, 472, 491
HpyAV CCTTC 3 cut(s) 368, 523, 550
HpyCH4IV ACGT 2 cut(s) 288, 324
HpyCH4V TGCA 2 cut(s) 29, 97
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpyF3I CTNAG 3 cut(s) 155, 494, 505
HpySE526I ACGT 2 cut(s) 288, 324
Hsp92II CATG 1 cut(s) 52
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 2 cut(s) 109, 343
MaeI CTAG 2 cut(s) 77, 254
MaeII ACGT 2 cut(s) 288, 324
MaeIII GTNAC 5 cut(s) 37, 168, 184, 289, 500
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 1 cut(s) 5
MlyI GAGTC 4 cut(s) 244, 286, 287, 496
MnlI CCTC 7 cut(s) 64, 150, 294, 309, 489, 517, 555
MseI TTAA 1 cut(s) 458
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 66, 424
MvaI CCWGG 2 cut(s) 66, 424
MwoI GCNNNNNNNGC 1 cut(s) 517
NdeI CATATG 1 cut(s) 466
NdeII GATC 1 cut(s) 318
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 18
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 4 cut(s) 37, 168, 184, 289
NspI RCATGY 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 345
PceI AGGCCT 1 cut(s) 64
PfeI GAWTC 2 cut(s) 206, 235
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 2 cut(s) 99, 333
PleI GAGTC 4 cut(s) 244, 286, 286, 495
PpsI GAGTC 4 cut(s) 244, 286, 286, 495
PsiI TTATAA 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 64, 422
PspGI CCWGG 2 cut(s) 64, 422
PspN4I GGNNCC 1 cut(s) 18
PstI CTGCAG 1 cut(s) 99
PstNI CAGNNNCTG 1 cut(s) 581
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 98, 332
Sau3AI GATC 1 cut(s) 318
SchI GAGTC 4 cut(s) 244, 286, 287, 496
ScrFI CCNGG 2 cut(s) 66, 424
SduI GDGCHC 1 cut(s) 405
SfcI CTRYAG 1 cut(s) 95
Sfr274I CTCGAG 1 cut(s) 345
SlaI CTCGAG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 1 cut(s) 5
SseBI AGGCCT 1 cut(s) 64
SspMI CTAG 2 cut(s) 77, 254
SstI GAGCTC 1 cut(s) 405
StuI AGGCCT 1 cut(s) 64
StyD4I CCNGG 2 cut(s) 64, 422
StyI CCWWGG 1 cut(s) 140
TaiI ACGT 2 cut(s) 291, 327
TaqI TCGA 1 cut(s) 346
TasI AATT 1 cut(s) 5
TfiI GAWTC 2 cut(s) 206, 235
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 4 cut(s) 37, 168, 184, 289
TseI GCWGC 2 cut(s) 97, 331
Tsp45I GTSAC 4 cut(s) 37, 168, 184, 289
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 146
XapI RAATTY 1 cut(s) 5
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 2 cut(s) 404, 430
XhoI CTCGAG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 490
XspI CTAG 2 cut(s) 77, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.