pycom03g19770

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028484
Physical Location & Seq
Forward (+)
20389809 .. 20391369
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g19770.4

Sequence Viewer

Length: 342 bp
ATGTTAATTATTCTCGATTCTTGCCTCCCTGTGCTTCACCTAATTGGCACTAGGCGTTCCATCCCGTGTGCTAACAAACAGATTCAAGAACTATTGGGAGTGTCTTGTGCTTTTGGTCAAGCAGTTCAGCGGGCATGCTTGATCACATCAGTAATACATTTTTGGATTGATTACGCGCTGGAAATTTTGAAGGAGCAGCTTATCCTCCTGGCAAATAGCATGACGTGCTGGAAATTTTGTCGGCTTAATTCTAGTGGATATAAAGCACTATCTCGAAGAGCAGCTGAAAAGTGCCACATGGACATGGATTCTTTGTCAAGCATAGTTGGATCCTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000419 GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_5

Protein Analysis

114

Amino Acids

12.56

Weight (kDa)

8.47

Isoelectric Point (pI)

68.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028484)

Species Orthologous Gene IDs
malus_domestica MD03G1248400.v1.1
pyrus_communis pycom03g19770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 130
AclWI GGATC 2 cut(s) 324, 337
AcsI RAATTY 2 cut(s) 183, 233
AgsI TTSAA 2 cut(s) 86, 190
AjiI CACGTC 1 cut(s) 225
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 199, 284
AluI AGCT 2 cut(s) 199, 284
AlwI GGATC 2 cut(s) 324, 337
ApeKI GCWGC 2 cut(s) 196, 281
ApoI RAATTY 2 cut(s) 183, 233
AspLEI GCGC 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 29
BamHI GGATCC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 208, 293
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 141
BfaI CTAG 2 cut(s) 51, 252
BisI GCNGC 2 cut(s) 197, 282
BlsI GCNGC 2 cut(s) 198, 283
Bme1390I CCNGG 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 225
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 208, 293
Bsh1236I CGCG 1 cut(s) 176
Bsp143I GATC 2 cut(s) 141, 329
BspACI CCGC 1 cut(s) 130
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 338
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 2 cut(s) 324, 337
BspQI GCTCTTC 1 cut(s) 271
BssMI GATC 2 cut(s) 141, 329
Bst2UI CCWGG 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 225
BstC8I GCNNGC 2 cut(s) 132, 136
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 2 cut(s) 144, 332
BstMBI GATC 2 cut(s) 141, 329
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 207
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 208, 293
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BtrI CACGTC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 60
Cac8I GCNNGC 2 cut(s) 132, 136
CciI TCATGA 1 cut(s) 338
CfoI GCGC 1 cut(s) 178
CviAII CATG 5 cut(s) 135, 220, 298, 304, 339
CviJI RGCY 3 cut(s) 199, 244, 284
CviKI_1 RGCY 3 cut(s) 199, 244, 284
DpnI GATC 2 cut(s) 143, 331
DpnII GATC 2 cut(s) 141, 329
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 207
FaeI CATG 5 cut(s) 138, 223, 301, 307, 342
FaiI YATR 7 cut(s) 136, 221, 261, 299, 305, 323, 340
FatI CATG 5 cut(s) 134, 219, 297, 303, 338
FauI CCCGC 1 cut(s) 123
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 197, 282
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 197, 282
FspBI CTAG 2 cut(s) 51, 252
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 197, 282
HhaI GCGC 1 cut(s) 178
Hin1II CATG 5 cut(s) 138, 223, 301, 307, 342
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 3 cut(s) 17, 82, 308
HphI GGTGA 1 cut(s) 29
Hpy188III TCNNGA 4 cut(s) 14, 86, 273, 339
HpyAV CCTTC 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 5 cut(s) 138, 223, 301, 307, 342
HspAI GCGC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 329
LguI GCTCTTC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 5 cut(s) 42, 164, 194, 214, 221
Lsp1109I GCAGC 2 cut(s) 208, 293
MaeI CTAG 2 cut(s) 51, 252
MaeII ACGT 1 cut(s) 224
MalI GATC 2 cut(s) 143, 331
MboI GATC 2 cut(s) 141, 329
MboII GAAGA 1 cut(s) 288
MflI RGATCY 1 cut(s) 329
MluCI AATT 5 cut(s) 6, 42, 183, 233, 247
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 2 cut(s) 35, 215
MseI TTAA 2 cut(s) 5, 246
MslI CAYNNNNRTG 1 cut(s) 302
MspA1I CMGCKG 2 cut(s) 130, 284
MspR9I CCNGG 1 cut(s) 209
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeII GATC 2 cut(s) 141, 329
NlaIII CATG 5 cut(s) 138, 223, 301, 307, 342
NlaIV GGNNCC 1 cut(s) 331
NspI RCATGY 1 cut(s) 138
PaeI GCATGC 1 cut(s) 138
PagI TCATGA 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 271
PfeI GAWTC 3 cut(s) 17, 82, 308
PkrI GCNGC 2 cut(s) 198, 283
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 331
PsuI RGATCY 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 2 cut(s) 5, 246
SatI GCNGC 2 cut(s) 197, 282
Sau3AI GATC 2 cut(s) 141, 329
ScrFI CCNGG 1 cut(s) 209
SetI ASST 4 cut(s) 42, 201, 227, 286
SmiMI CAYNNNNRTG 1 cut(s) 302
SphI GCATGC 1 cut(s) 138
Sse9I AATT 5 cut(s) 6, 42, 183, 233, 247
SsiI CCGC 1 cut(s) 130
SspMI CTAG 2 cut(s) 51, 252
StyD4I CCNGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 227
TaqI TCGA 2 cut(s) 15, 274
TasI AATT 5 cut(s) 6, 42, 183, 233, 247
TfiI GAWTC 3 cut(s) 17, 82, 308
Tru1I TTAA 2 cut(s) 5, 246
Tru9I TTAA 2 cut(s) 5, 246
TseI GCWGC 2 cut(s) 196, 281
TspDTI ATGAA 1 cut(s) 327
XapI RAATTY 2 cut(s) 183, 233
XceI RCATGY 1 cut(s) 138
XspI CTAG 2 cut(s) 51, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.