pycom03g23700

Replication protein A interacting middle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0019180
Physical Location & Seq
Forward (+)
23540440 .. 23543083
2644 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGTTACCAATAAAAAATAAACACGTTAATCAATACTTAAGTAATAATCTTAAGAAGGCTCAGAAGGAAACGTGGAGAAGAGATAGAGCGGTGAGACAAAGGGAAATGGAGGGAGAAGCCGCAGCCGCAGCAGAAGGGCGGCATCCTCTCAAACCCCATCCTCTTTTCAATAACCATAACAGCTGGAAACATAAGCCTTCCGAGAGAGAACTGTTACAAAAGGGTTCGAGAAGACAGAAGGCGGTTGCTTTGGAAGATGAGAATGCTGCCCACTCCACAAATTCTCAATCATATTCCAAATGGTTTGATCGATCTGCATTCCAGGACATAGTTTCTGCTGAATTGGAAAAAATTAAGGAGTCGTCATTGGATGAAAATGTAAGGAGTTCAACATCTGTCCCTGAGGCCAATGATGTGTTATGGGAATACGATGGCCTTACAAATATATACCAAGGAGATTGTGAAGAGATATTGTTAGCAATGCAAAGGATCTTTTATGAAGACCTGACTACTCATCCAACTAGAAAAGAAGAAACAGAAATATGTATTGAAACATGGGAAGATGAAGAGGATGAATATTTGTCCCAGGCAGTTTATGAGCATATGCAACTGAATGACAAGCAGATGGGCAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPA_interact_C RPA_interact_M RPA_interact_N

Protein Analysis

213

Amino Acids

24.82

Weight (kDa)

5.29

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPA_interact_M PF14767 105 - 198 7e-25 Replication protein A interacting middle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12760
malus_domestica MD03G1298900.v1.1 MD11G1318200.v1.1
prunus_persica Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1
pyrus_communis pycom03g23700 pycom11g27740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 89
AciI CCGC 5 cut(s) 89, 120, 126, 139, 242
AclWI GGATC 1 cut(s) 497
AcsI RAATTY 1 cut(s) 280
AflII CTTAAG 2 cut(s) 37, 50
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 3 cut(s) 169, 390, 551
AjnI CCWGG 2 cut(s) 321, 585
AluBI AGCT 1 cut(s) 183
AluI AGCT 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 497
AoxI GGCC 2 cut(s) 405, 433
ApeKI GCWGC 3 cut(s) 122, 128, 266
ApoI RAATTY 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 103
AxyI CCTNAGG 1 cut(s) 402
BbsI GAAGAC 2 cut(s) 238, 507
BbvI GCAGC 3 cut(s) 134, 140, 253
BccI CCATC 3 cut(s) 165, 425, 619
BciT130I CCWGG 2 cut(s) 323, 587
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 522
BfrI CTTAAG 2 cut(s) 37, 50
BisI GCNGC 6 cut(s) 120, 123, 126, 129, 140, 267
BlsI GCNGC 6 cut(s) 121, 124, 127, 130, 141, 268
Bme1390I CCNGG 2 cut(s) 323, 587
BmrFI CCNGG 2 cut(s) 323, 587
BmsI GCATC 1 cut(s) 151
BpiI GAAGAC 2 cut(s) 238, 507
Bsa29I ATCGAT 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 451, 585
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 2 cut(s) 323, 587
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 2 cut(s) 451, 585
BseGI GGATG 5 cut(s) 142, 157, 376, 514, 577
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 2 cut(s) 74, 393
BseXI GCAGC 3 cut(s) 134, 140, 253
BshFI GGCC 2 cut(s) 407, 435
BshVI ATCGAT 1 cut(s) 310
BslFI GGGAC 2 cut(s) 383, 568
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 2 cut(s) 383, 568
BsmI GAATGC 2 cut(s) 268, 317
BsnI GGCC 2 cut(s) 407, 435
Bsp143I GATC 3 cut(s) 307, 311, 489
BspACI CCGC 5 cut(s) 89, 120, 126, 139, 242
BspANI GGCC 2 cut(s) 407, 435
BspCNI CTCAG 2 cut(s) 73, 394
BspDI ATCGAT 1 cut(s) 310
BspPI GGATC 1 cut(s) 497
BspTI CTTAAG 2 cut(s) 37, 50
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 486
BssECI CCNNGG 2 cut(s) 451, 585
BssMI GATC 3 cut(s) 307, 311, 489
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 2 cut(s) 323, 587
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 3 cut(s) 73, 459, 561
BstAFI CTTAAG 2 cut(s) 37, 50
BstDEI CTNAG 2 cut(s) 60, 402
BstF5I GGATG 5 cut(s) 142, 157, 376, 514, 577
BstKTI GATC 3 cut(s) 310, 314, 492
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 3 cut(s) 307, 311, 489
BstMWI GCNNNNNNNGC 2 cut(s) 125, 128
BstNI CCWGG 2 cut(s) 323, 587
BstSCI CCNGG 2 cut(s) 321, 585
BstV1I GCAGC 3 cut(s) 134, 140, 253
BstV2I GAAGAC 2 cut(s) 238, 507
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
Bsu15I ATCGAT 1 cut(s) 310
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 2 cut(s) 407, 435
BsuTUI ATCGAT 1 cut(s) 310
BtsCI GGATG 5 cut(s) 142, 157, 376, 514, 577
ClaI ATCGAT 1 cut(s) 310
CviAII CATG 1 cut(s) 555
CviJI RGCY 7 cut(s) 59, 119, 125, 183, 196, 407, 435
CviKI_1 RGCY 7 cut(s) 59, 119, 125, 183, 196, 407, 435
DdeI CTNAG 2 cut(s) 60, 402
DpnI GATC 3 cut(s) 309, 313, 491
DpnII GATC 3 cut(s) 307, 311, 489
Eam1104I CTCTTC 3 cut(s) 73, 459, 561
EarI CTCTTC 3 cut(s) 73, 459, 561
Eco130I CCWWGG 1 cut(s) 451
Eco81I CCTNAGG 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 321, 585
EcoT14I CCWWGG 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 1 cut(s) 558
FaqI GGGAC 2 cut(s) 383, 568
FatI CATG 1 cut(s) 554
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 6 cut(s) 120, 123, 126, 129, 140, 267
FokI GGATG 5 cut(s) 129, 144, 383, 501, 584
Fsp4HI GCNGC 6 cut(s) 120, 123, 126, 129, 140, 267
FspBI CTAG 1 cut(s) 522
GluI GCNGC 6 cut(s) 120, 123, 126, 129, 140, 267
HaeIII GGCC 2 cut(s) 407, 435
Hin1II CATG 1 cut(s) 558
HinfI GANTC 1 cut(s) 359
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 63, 202
Hpy188III TCNNGA 1 cut(s) 228
HpyAV CCTTC 5 cut(s) 49, 58, 128, 207, 232
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 2 cut(s) 24, 71
HpyCH4V TGCA 3 cut(s) 317, 484, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 128
HpyF3I CTNAG 2 cut(s) 60, 402
HpySE526I ACGT 2 cut(s) 24, 71
Hsp92II CATG 1 cut(s) 558
Kzo9I GATC 3 cut(s) 307, 311, 489
LpnPI CCDG 7 cut(s) 169, 308, 335, 414, 518, 572, 599
Lsp1109I GCAGC 3 cut(s) 134, 140, 253
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 522
MaeII ACGT 2 cut(s) 24, 71
MaeIII GTNAC 2 cut(s) 3, 213
MalI GATC 3 cut(s) 309, 313, 491
MbiI CCGCTC 1 cut(s) 89
MboI GATC 3 cut(s) 307, 311, 489
MboII GAAGA 8 cut(s) 90, 243, 266, 476, 512, 542, 572, 578
MflI RGATCY 1 cut(s) 489
MluCI AATT 3 cut(s) 280, 341, 351
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 5 cut(s) 103, 156, 171, 397, 562
MseI TTAA 4 cut(s) 27, 38, 51, 354
MspA1I CMGCKG 1 cut(s) 183
MspCI CTTAAG 2 cut(s) 37, 50
MspR9I CCNGG 2 cut(s) 323, 587
Mva1269I GAATGC 2 cut(s) 268, 317
MvaI CCWGG 2 cut(s) 323, 587
MwoI GCNNNNNNNGC 2 cut(s) 125, 128
NdeI CATATG 1 cut(s) 603
NdeII GATC 3 cut(s) 307, 311, 489
NlaIII CATG 1 cut(s) 558
PctI GAATGC 2 cut(s) 268, 317
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 6 cut(s) 121, 124, 127, 130, 141, 268
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 321, 585
PspGI CCWGG 2 cut(s) 321, 585
PsuI RGATCY 1 cut(s) 489
PvuII CAGCTG 1 cut(s) 183
SaqAI TTAA 4 cut(s) 27, 38, 51, 354
SatI GCNGC 6 cut(s) 120, 123, 126, 129, 140, 267
Sau3AI GATC 3 cut(s) 307, 311, 489
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 323, 587
SetI ASST 4 cut(s) 27, 74, 185, 507
SfaNI GCATC 1 cut(s) 151
SmlI CTYRAG 2 cut(s) 37, 50
SmoI CTYRAG 2 cut(s) 37, 50
Sse9I AATT 3 cut(s) 280, 341, 351
SsiI CCGC 5 cut(s) 89, 120, 126, 139, 242
SspI AATATT 1 cut(s) 578
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 321, 585
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 2 cut(s) 27, 74
TaqI TCGA 2 cut(s) 227, 310
TasI AATT 3 cut(s) 280, 341, 351
TauI GCSGC 3 cut(s) 122, 128, 142
Tru1I TTAA 4 cut(s) 27, 38, 51, 354
Tru9I TTAA 4 cut(s) 27, 38, 51, 354
TseI GCWGC 3 cut(s) 122, 128, 266
TspDTI ATGAA 4 cut(s) 387, 513, 579, 588
Vha464I CTTAAG 2 cut(s) 37, 50
XapI RAATTY 1 cut(s) 280
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.