pycom04g00850

Small ubiquitinrelated modifier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0028685
Physical Location & Seq
Forward (+)
750112 .. 750662
551 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 243 bp
ATGAGATCCCCTTGCAGGATGGAAGTGAAGTGTTTTTCAGGATTAAACCAACTACAAAGCATTGATGCATTGTGCACAAAACGTTCCCTGGATCGCAATTCCTTAGTGTTCATTTTCGATGGTGATCACATTAATGGCAAGAAGACTGCTGCGGAGCATCATATGGAAGATGGTGATGAAATCGATGTCTTTCTTCTCACTGATGGAGGTTCAGTGACATTCAAGCTTGTCATGATGAACTGA

Protein Analysis

81

Amino Acids

8.98

Weight (kDa)

5.44

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 21 - 64 4.4e-11 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028685)

Species Orthologous Gene IDs
malus_domestica MD04G1010400.v1.1
pyrus_communis pycom04g00850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 152
AclI AACGTT 1 cut(s) 82
AclWI GGATC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 77
Alw44I GTGCAC 1 cut(s) 73
AlwI GGATC 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 149
AseI ATTAAT 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 134, 185
BaeGI GKGCMC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 136
BccI CCATC 4 cut(s) 13, 113, 164, 197
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 124
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 55, 166
BpiI GAAGAC 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 1 cut(s) 89
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 24
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 5, 91, 124
BspACI CCGC 1 cut(s) 152
BspDI ATCGAT 1 cut(s) 183
BspHI TCATGA 1 cut(s) 231
BspPI GGATC 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 3 cut(s) 5, 91, 124
Bst2UI CCWGG 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 103
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 3 cut(s) 8, 94, 127
BstMBI GATC 3 cut(s) 5, 91, 124
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 136
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
Bsu15I ATCGAT 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 183
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 198, 219
CciI TCATGA 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 183
CviAII CATG 1 cut(s) 232
CviJI RGCY 1 cut(s) 226
CviKI_1 RGCY 1 cut(s) 226
DdeI CTNAG 1 cut(s) 103
DpnI GATC 3 cut(s) 7, 93, 126
DpnII GATC 3 cut(s) 5, 91, 124
EcoRII CCWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 70
FaeI CATG 1 cut(s) 235
FaiI YATR 3 cut(s) 162, 164, 233
FatI CATG 1 cut(s) 231
FauNDI CATATG 1 cut(s) 162
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
Hin1II CATG 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 224
HphI GGTGA 2 cut(s) 134, 185
Hpy166II GTNNAC 1 cut(s) 75
Hpy188III TCNNGA 2 cut(s) 39, 232
Hpy8I GTNNAC 1 cut(s) 75
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 3 cut(s) 15, 68, 75
HpyF3I CTNAG 1 cut(s) 103
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 3 cut(s) 5, 91, 124
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 3 cut(s) 24, 74, 101
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 2 cut(s) 55, 166
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 7, 93, 126
MboI GATC 3 cut(s) 5, 91, 124
MboII GAAGA 3 cut(s) 154, 179, 185
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 1 cut(s) 97
MnlI CCTC 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 70
MseI TTAA 2 cut(s) 44, 132
MslI CAYNNNNRTG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
NdeI CATATG 1 cut(s) 162
NdeII GATC 3 cut(s) 5, 91, 124
NlaIII CATG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 70
PagI TCATGA 1 cut(s) 231
PkrI GCNGC 1 cut(s) 151
PshBI ATTAAT 1 cut(s) 132
Psp1406I AACGTT 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PsuI RGATCY 1 cut(s) 5
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 2 cut(s) 44, 132
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 3 cut(s) 5, 91, 124
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 77
SetI ASST 3 cut(s) 85, 211, 228
SfaNI GCATC 2 cut(s) 55, 166
SgeI CNNG 8 cut(s) 24, 28, 51, 100, 101, 151, 235, 239
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 1 cut(s) 97
SsiI CCGC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 85
TaqI TCGA 2 cut(s) 117, 183
TasI AATT 1 cut(s) 97
Tru1I TTAA 2 cut(s) 44, 132
Tru9I TTAA 2 cut(s) 44, 132
TscAI CASTG 2 cut(s) 205, 219
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 100, 192
TspRI CASTG 2 cut(s) 205, 219
VneI GTGCAC 1 cut(s) 73
VspI ATTAAT 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.