pycom04g04010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0024409
Physical Location & Seq
Reverse (-)
3591216 .. 3594617
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1548 bp
ATGGCTTCCACATATAGCACATCGTTAGTTGTTTCCATGATGCTGATTTTATCCTTGAGGATGATCACAGAAATACAAGGACAGTCTACTGGGTCGACCAACTTTGCACCCCCACCACAAGTACCAGGGAGCAATTTGCTTCAGGCAAACAATGATTCAGAGCCCTTGACACAGTCAGATGATACTGTAAGGGTGGATCCTTTAGATGATTTGAAAAAATATAGAGGAGGATACAACATTACCAACAAGCACTACTGGAGTTCAACGATATTTACTGGAATTCATGGATATGCACTTGGGATGCTGTGGCTTTTGTGTGGAATTTTATATGGATGCTTCCTATTAGCAACCAAACTTTGTTGTAAAAATAGGAAGAGTGGAAAGCTAAAGAAAAGATTACTTTGTCATAAGCAGTGTTACCTCTGGCAATGGCACATTTTCCTCGCCATATTCTTCACCATCTTTGCAATAGTTGCATTCGGATTAGTTTTAGGGGGCAACGCGAGATTTCATTCACAAGCAAAATCAGTGGTGGACATTATCATAACTACTGCAAATGAAGCATCAGGTACCGTGTACAACACAACAGGAGCCATGAAAGAAATGAGCAACAACTTTGATTCAACTGGAAACACTGAGGTTTCGAGCTTCCTCACCTCCACATCCCAAAAGCTTGATACTGCAGCTGCAGATATAGAAAGGCAGGCTAGGAAGAACCGGCGTTTGATTGACAAGGGTCTCAAGATAGTGTATATTGTAACAACAGTGCCTATTTCCTTGGGCCTGGTTGCAGTGATTGCTCTGTCAGGAATGTTAATTGAAAACCGTTTGTTCGATTTCAGGTTCTCAAACGATACATGCACAGCTCTCGAAAGTTTCCAACAAAATCCGAACAACAACAGCTTGAGCTCAATCCTCCCCTGCGATGAATTGGTCTCAGCAAAATCGCTTCTAAATGATGTTGGTGCCGGGATCTATAGACTTGTTAATGAGGTAAATGCAAACATATCATCTCTACAGGAAACATCATCTCAGAATATAGAATATGTCTGCAATCCCTTCTCAGAACCACCAGAATACAAATACCAACCACAAAAATGTCCAAATAATTCCGTTCCAATAGGAGACATCCCAGAGGTCTTGAGAGTAGCTACTTGTTCAGACGCCAACAGTGGAACCTGCGAGAATGGAACAGTTATATCGGACAGCGCTTACAAACTGGTGGAACTCTACACAAGTTCAATACAAGGTATACTAAATTCATACCCAGGAATGGAAAGCCTAGTAGAATGTCAGACAGTGAAGGATGCATTTTCTGAAATCCTTGTCAAACACTGCAAACCGTTGAAGCGATACGCTAAGATGGTTTGGGCAGCAATGGCCTTTCTTGCAACACTAATGGTGTTGTTGGTTCTGTTATGGATAATACAAGCCAATCATGAACGAAACCACCATTCTGCAGATGGTTCTGTGTCTGCAACAGAAAATGTGATGGAGTTGGGCGCCGCTACTGCCGTTAAGGACAACCCAAATCCTAGCTTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

516

Amino Acids

56.5

Weight (kDa)

6.51

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024409)

Species Orthologous Gene IDs
malus_domestica MD04G1046200.v1.1
prunus_persica Prupe.1G199700_v2.0.a1
pyrus_communis pycom04g04010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1187
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 3 cut(s) 569, 965, 1502
AccI GTMKAC 3 cut(s) 86, 95, 1252
AccII CGCG 1 cut(s) 503
AciI CCGC 1 cut(s) 1506
AclWI GGATC 3 cut(s) 191, 204, 980
AcsI RAATTY 3 cut(s) 279, 321, 1258
AcuI CTGAAG 1 cut(s) 125
AcyI GRCGYC 2 cut(s) 1164, 1503
AfaI GTAC 3 cut(s) 123, 571, 578
AfeI AGCGCT 1 cut(s) 1210
AfiI CCNNNNNNNGG 1 cut(s) 1273
AgsI TTSAA 6 cut(s) 214, 264, 624, 821, 1242, 1348
AjnI CCWGG 3 cut(s) 124, 783, 1267
AluBI AGCT 9 cut(s) 385, 648, 673, 686, 866, 903, 909, 1151, 1539
AluI AGCT 9 cut(s) 385, 648, 673, 686, 866, 903, 909, 1151, 1539
Alw21I GWGCWC 1 cut(s) 911
Alw26I GTCTC 3 cut(s) 743, 940, 1119
AlwI GGATC 3 cut(s) 191, 204, 980
Aor51HI AGCGCT 1 cut(s) 1210
AoxI GGCC 2 cut(s) 781, 1380
ApeKI GCWGC 3 cut(s) 683, 686, 1373
ApoI RAATTY 3 cut(s) 279, 321, 1258
Asp718I GGTACC 1 cut(s) 569
AspLEI GCGC 2 cut(s) 1211, 1505
AspS9I GGNCC 1 cut(s) 781
AsuC2I CCSGG 1 cut(s) 970
AsuHPI GGTGA 2 cut(s) 448, 646
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 3 cut(s) 569, 965, 1502
BanII GRGCYC 2 cut(s) 165, 911
Bbv12I GWGCWC 1 cut(s) 911
BbvI GCAGC 3 cut(s) 673, 695, 1385
BccI CCATC 4 cut(s) 467, 1357, 1457, 1486
BceAI ACGGC 1 cut(s) 1499
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 3 cut(s) 126, 785, 1269
BciVI GTATCC 1 cut(s) 224
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 1 cut(s) 970
BcoDI GTCTC 3 cut(s) 743, 940, 1119
BfaI CTAG 3 cut(s) 708, 1283, 1536
BfmI CTRYAG 5 cut(s) 681, 687, 976, 1016, 1458
BfoI RGCGCY 2 cut(s) 1212, 1506
BfuAI ACCTGC 1 cut(s) 1187
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 4 cut(s) 684, 687, 1374, 1506
BlpI GCTNAGC 1 cut(s) 1540
BlsI GCNGC 4 cut(s) 685, 688, 1375, 1507
Bme1390I CCNGG 4 cut(s) 126, 785, 970, 1269
BmgT120I GGNCC 1 cut(s) 781
BmiI GGNNCC 6 cut(s) 198, 571, 592, 967, 1177, 1504
BmrFI CCNGG 4 cut(s) 126, 785, 970, 1269
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 5 cut(s) 30, 291, 323, 572, 1297
BmuI ACTGGG 1 cut(s) 99
BoxI GACNNNNGTC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 1540
BpuEI CTTGAG 4 cut(s) 76, 725, 925, 1162
BpuMI CCSGG 1 cut(s) 970
BsaHI GRCGYC 2 cut(s) 1164, 1503
BsaI GGTCTC 2 cut(s) 743, 940
BsaJI CCNNGG 3 cut(s) 125, 777, 1267
Bsc4I CCNNNNNNNGG 1 cut(s) 1273
Bse118I RCCGGY 1 cut(s) 717
Bse1I ACTGG 5 cut(s) 94, 260, 280, 631, 1224
Bse3DI GCAATG 2 cut(s) 434, 1383
BseBI CCWGG 3 cut(s) 126, 785, 1269
BseDI CCNNGG 3 cut(s) 125, 777, 1267
BseGI GGATG 6 cut(s) 66, 306, 338, 662, 1128, 1312
BseLI CCNNNNNNNGG 1 cut(s) 1273
BseMI GCAATG 2 cut(s) 434, 1383
BseMII CTCAG 4 cut(s) 627, 951, 1046, 1077
BseNI ACTGG 5 cut(s) 94, 260, 280, 631, 1224
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 673, 695, 1385
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 2 cut(s) 783, 1382
BshNI GGYRCC 3 cut(s) 569, 965, 1502
BsiHKAI GWGCWC 1 cut(s) 911
BsiSI CCGG 2 cut(s) 718, 969
BslI CCNNNNNNNGG 1 cut(s) 1273
BsmAI GTCTC 3 cut(s) 743, 940, 1119
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 783, 1382
Bso31I GGTCTC 2 cut(s) 743, 940
Bsp1286I GDGCHC 2 cut(s) 165, 911
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 3 cut(s) 63, 196, 972
Bsp1720I GCTNAGC 1 cut(s) 1540
BspACI CCGC 1 cut(s) 1506
BspANI GGCC 2 cut(s) 783, 1382
BspCNI CTCAG 4 cut(s) 628, 950, 1045, 1076
BspFNI CGCG 1 cut(s) 503
BspHI TCATGA 1 cut(s) 1438
BspLI GGNNCC 6 cut(s) 198, 571, 592, 967, 1177, 1504
BspMAI CTGCAG 3 cut(s) 685, 691, 1462
BspMI ACCTGC 1 cut(s) 1187
BspPI GGATC 3 cut(s) 191, 204, 980
BspT107I GGYRCC 3 cut(s) 569, 965, 1502
BspTNI GGTCTC 2 cut(s) 743, 940
BsrDI GCAATG 2 cut(s) 434, 1383
BsrFI RCCGGY 1 cut(s) 717
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 5 cut(s) 94, 260, 280, 631, 1224
BssAI RCCGGY 1 cut(s) 717
BssECI CCNNGG 3 cut(s) 125, 777, 1267
BssMI GATC 3 cut(s) 63, 196, 972
BssNAI GTATAC 1 cut(s) 1253
BssNI GRCGYC 2 cut(s) 1164, 1503
BssT1I CCWWGG 1 cut(s) 777
Bst1107I GTATAC 1 cut(s) 1253
Bst2UI CCWGG 3 cut(s) 126, 785, 1269
Bst6I CTCTTC 1 cut(s) 368
BstACI GRCGYC 2 cut(s) 1164, 1503
BstAPI GCANNNNNTGC 2 cut(s) 473, 797
BstAUI TGTACA 1 cut(s) 576
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 6 cut(s) 636, 937, 1032, 1063, 1359, 1540
BstF5I GGATG 6 cut(s) 66, 306, 338, 662, 1128, 1312
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 2 cut(s) 1212, 1506
BstHHI GCGC 2 cut(s) 1211, 1505
BstKTI GATC 3 cut(s) 66, 199, 975
BstMAI GTCTC 3 cut(s) 743, 940, 1119
BstMBI GATC 3 cut(s) 63, 196, 972
BstMWI GCNNNNNNNGC 6 cut(s) 473, 560, 797, 1379, 1388, 1511
BstNI CCWGG 3 cut(s) 126, 785, 1269
BstNSI RCATGY 1 cut(s) 861
BstPAI GACNNNNGTC 1 cut(s) 735
BstSCI CCNGG 4 cut(s) 124, 783, 968, 1267
BstSFI CTRYAG 5 cut(s) 681, 687, 976, 1016, 1458
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 3 cut(s) 673, 695, 1385
BstX2I RGATCY 2 cut(s) 196, 972
BstYI RGATCY 2 cut(s) 196, 972
BstZ17I GTATAC 1 cut(s) 1253
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 2 cut(s) 783, 1382
BtgZI GCGATG 1 cut(s) 939
BtsCI GGATG 6 cut(s) 66, 306, 338, 662, 1128, 1312
BtsI GCAGTG 3 cut(s) 419, 798, 1333
BtsIMutI CAGTG 8 cut(s) 419, 534, 633, 771, 798, 1177, 1305, 1333
BveI ACCTGC 1 cut(s) 1187
Cac8I GCNNGC 1 cut(s) 705
CciI TCATGA 1 cut(s) 1438
CfoI GCGC 2 cut(s) 1211, 1505
Cfr10I RCCGGY 1 cut(s) 717
Cfr13I GGNCC 1 cut(s) 781
CseI GACGC 1 cut(s) 1172
Csp6I GTAC 3 cut(s) 122, 570, 577
CspCI CAANNNNNGTGG 2 cut(s) 510, 545
CviAII CATG 5 cut(s) 37, 284, 595, 858, 1439
CviQI GTAC 3 cut(s) 122, 570, 577
DdeI CTNAG 6 cut(s) 636, 937, 1032, 1063, 1359, 1540
DinI GGCGCC 1 cut(s) 1504
DpnI GATC 3 cut(s) 65, 198, 974
DpnII GATC 3 cut(s) 63, 196, 972
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 909
Eco130I CCWWGG 1 cut(s) 777
Eco24I GRGCYC 2 cut(s) 165, 911
Eco31I GGTCTC 2 cut(s) 743, 940
Eco47III AGCGCT 1 cut(s) 1210
Eco53kI GAGCTC 1 cut(s) 909
Eco57I CTGAAG 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 909
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 3 cut(s) 124, 783, 1267
EcoT14I CCWWGG 1 cut(s) 777
EcoT22I ATGCAT 1 cut(s) 1312
EcoT38I GRGCYC 2 cut(s) 165, 911
EgeI GGCGCC 1 cut(s) 1504
EheI GGCGCC 1 cut(s) 1504
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 5 cut(s) 40, 287, 598, 861, 1442
FatI CATG 5 cut(s) 36, 283, 594, 857, 1438
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 3 cut(s) 86, 95, 1252
Fnu4HI GCNGC 4 cut(s) 684, 687, 1374, 1506
FokI GGATG 6 cut(s) 73, 313, 345, 649, 1115, 1319
FriOI GRGCYC 2 cut(s) 165, 911
Fsp4HI GCNGC 4 cut(s) 684, 687, 1374, 1506
FspBI CTAG 3 cut(s) 708, 1283, 1536
GlaI GCGC 2 cut(s) 1210, 1504
GluI GCNGC 4 cut(s) 684, 687, 1374, 1506
GsuI CTGGAG 1 cut(s) 277
HaeII RGCGCY 2 cut(s) 1212, 1506
HaeIII GGCC 2 cut(s) 783, 1382
HapII CCGG 2 cut(s) 718, 969
HgaI GACGC 1 cut(s) 1172
HhaI GCGC 2 cut(s) 1211, 1505
Hin1I GRCGYC 2 cut(s) 1164, 1503
Hin1II CATG 5 cut(s) 40, 287, 598, 861, 1442
Hin6I GCGC 2 cut(s) 1209, 1503
HinP1I GCGC 2 cut(s) 1209, 1503
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 2 cut(s) 155, 620
HpaII CCGG 2 cut(s) 718, 969
HphI GGTGA 2 cut(s) 448, 646
Hpy166II GTNNAC 5 cut(s) 87, 96, 535, 577, 1253
Hpy188III TCNNGA 5 cut(s) 742, 807, 869, 1141, 1439
Hpy8I GTNNAC 5 cut(s) 87, 96, 535, 577, 1253
HpyAV CCTTC 2 cut(s) 1069, 1297
HpyF10VI GCNNNNNNNGC 6 cut(s) 473, 560, 797, 1379, 1388, 1511
HpyF3I CTNAG 6 cut(s) 636, 937, 1032, 1063, 1359, 1540
Hsp92I GRCGYC 2 cut(s) 1164, 1503
Hsp92II CATG 5 cut(s) 40, 287, 598, 861, 1442
HspAI GCGC 2 cut(s) 1209, 1503
KasI GGCGCC 1 cut(s) 1502
KpnI GGTACC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 3 cut(s) 63, 196, 972
LmnI GCTCC 2 cut(s) 129, 590
Lsp1109I GCAGC 3 cut(s) 673, 695, 1385
LweI GCATC 5 cut(s) 30, 291, 323, 572, 1297
MaeI CTAG 3 cut(s) 708, 1283, 1536
MaeIII GTNAC 2 cut(s) 416, 757
MalI GATC 3 cut(s) 65, 198, 974
MboI GATC 3 cut(s) 63, 196, 972
MboII GAAGA 3 cut(s) 385, 445, 724
MflI RGATCY 2 cut(s) 196, 972
MhlI GDGCHC 2 cut(s) 165, 911
MluCI AATT 7 cut(s) 133, 279, 321, 816, 929, 1108, 1258
Mly113I GGCGCC 1 cut(s) 1503
MmeI TCCRAC 1 cut(s) 904
Mph1103I ATGCAT 1 cut(s) 1312
MseI TTAA 3 cut(s) 815, 987, 1518
MslI CAYNNNNRTG 2 cut(s) 288, 1096
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 2 cut(s) 718, 969
MspR9I CCNGG 4 cut(s) 126, 785, 970, 1269
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 3 cut(s) 126, 785, 1269
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 6 cut(s) 473, 560, 797, 1379, 1388, 1511
NarI GGCGCC 1 cut(s) 1503
NciI CCSGG 1 cut(s) 970
NdeII GATC 3 cut(s) 63, 196, 972
NlaIII CATG 5 cut(s) 40, 287, 598, 861, 1442
NlaIV GGNNCC 6 cut(s) 198, 571, 592, 967, 1177, 1504
NsiI ATGCAT 1 cut(s) 1312
NspI RCATGY 1 cut(s) 861
PagI TCATGA 1 cut(s) 1438
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 155, 620
PflFI GACNNNGTC 1 cut(s) 172
PkrI GCNGC 4 cut(s) 685, 688, 1375, 1507
PluTI GGCGCC 1 cut(s) 1506
PshAI GACNNNNGTC 1 cut(s) 735
Psp124BI GAGCTC 1 cut(s) 911
Psp6I CCWGG 3 cut(s) 124, 783, 1267
PspGI CCWGG 3 cut(s) 124, 783, 1267
PspN4I GGNNCC 6 cut(s) 198, 571, 592, 967, 1177, 1504
PspPI GGNCC 1 cut(s) 781
PstI CTGCAG 3 cut(s) 685, 691, 1462
PsuI RGATCY 2 cut(s) 196, 972
PsyI GACNNNGTC 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 3 cut(s) 123, 571, 578
RsaNI GTAC 3 cut(s) 122, 570, 577
RseI CAYNNNNRTG 2 cut(s) 288, 1096
SacI GAGCTC 1 cut(s) 911
SalI GTCGAC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 815, 987, 1518
SatI GCNGC 4 cut(s) 684, 687, 1374, 1506
Sau3AI GATC 3 cut(s) 63, 196, 972
Sau96I GGNCC 1 cut(s) 781
ScrFI CCNGG 4 cut(s) 126, 785, 970, 1269
SduI GDGCHC 2 cut(s) 165, 911
SfaNI GCATC 5 cut(s) 30, 291, 323, 572, 1297
SfcI CTRYAG 5 cut(s) 681, 687, 976, 1016, 1458
SfoI GGCGCC 1 cut(s) 1504
SmiMI CAYNNNNRTG 2 cut(s) 288, 1096
SmlI CTYRAG 4 cut(s) 55, 740, 904, 1141
SmoI CTYRAG 4 cut(s) 55, 740, 904, 1141
Sse9I AATT 7 cut(s) 133, 279, 321, 816, 929, 1108, 1258
SsiI CCGC 1 cut(s) 1506
SspDI GGCGCC 1 cut(s) 1502
SspMI CTAG 3 cut(s) 708, 1283, 1536
SstI GAGCTC 1 cut(s) 911
StyD4I CCNGG 4 cut(s) 124, 783, 968, 1267
StyI CCWWGG 1 cut(s) 777
TaqI TCGA 4 cut(s) 95, 644, 834, 870
TasI AATT 7 cut(s) 133, 279, 321, 816, 929, 1108, 1258
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 1508
TfiI GAWTC 2 cut(s) 155, 620
Tru1I TTAA 3 cut(s) 815, 987, 1518
Tru9I TTAA 3 cut(s) 815, 987, 1518
TscAI CASTG 8 cut(s) 419, 534, 640, 771, 798, 1177, 1305, 1340
TseI GCWGC 3 cut(s) 683, 686, 1373
TspDTI ATGAA 7 cut(s) 272, 500, 573, 611, 942, 1251, 1455
TspGWI ACGGA 1 cut(s) 1102
TspRI CASTG 8 cut(s) 419, 534, 640, 771, 798, 1177, 1305, 1340
Tth111I GACNNNGTC 1 cut(s) 172
XapI RAATTY 3 cut(s) 279, 321, 1258
XceI RCATGY 1 cut(s) 861
XcmI CCANNNNNNNNNTGG 1 cut(s) 1460
XmiI GTMKAC 3 cut(s) 86, 95, 1252
XspI CTAG 3 cut(s) 708, 1283, 1536
Zsp2I ATGCAT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.