pycom04g06460

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0021260
Physical Location & Seq
Reverse (-)
6506269 .. 6506646
378 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06460.1

Sequence Viewer

Length: 378 bp
ATGGCCAATGGCACAGCAGCTGGCGTGGCACCTTATGCTCACGTGGCAATGTACAAGGTATATGGTGAGGAGGGTTGTTCTGATGCATACATTTTAGCGGCTATGGATACTGCTGTCAAAGATGGCGTTGACATCCTCTCCCTCTCACTTGCTGGTACTTCATTTCCTTTCTATAATGAAGGAATTGATGTAGGTGCATTCACAACAATCCAAAAGGGAATTTTTGTCAGCTGTTCAACAGGAAATTCTGGTCCTGACTACGAGTCTTTGTCCAACGAGGCCCCGTGGATTCTCACAGTTGGAGCAAGCACCATTGACAGAAGCATAAGAGCGACAGCACTCATTAGAAATCACGGGGAACTTGATGCGTATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

126

Amino Acids

13.09

Weight (kDa)

4.36

Isoelectric Point (pI)

21.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 6 - 104 1.5e-11 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021260)

Species Orthologous Gene IDs
pyrus_communis pycom04g06460
rosa_chinensis RchiOBHm_Chr7g0200041 RchiOBHm_Chr7g0200131
rosa_samantha Rh7DG186600 Rh7DG187700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 219, 244
AcvI CACGTG 1 cut(s) 43
AfaI GTAC 2 cut(s) 53, 157
AgsI TTSAA 1 cut(s) 237
AhdI GACNNNNNGTC 1 cut(s) 262
AluBI AGCT 2 cut(s) 20, 231
AluI AGCT 2 cut(s) 20, 231
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 2 cut(s) 3, 279
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 2 cut(s) 219, 244
AspS9I GGNCC 2 cut(s) 251, 280
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 251
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 28
BbrPI CACGTG 1 cut(s) 43
BbvI GCAGC 1 cut(s) 29
BccI CCATC 1 cut(s) 116
BciVI GTATCC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 2 cut(s) 18, 99
BlsI GCNGC 2 cut(s) 19, 100
Bme18I GGWCC 1 cut(s) 251
BmeRI GACNNNNNGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 251, 280
BmiI GGNNCC 2 cut(s) 30, 282
BmsI GCATC 2 cut(s) 73, 355
BsaAI YACGTR 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 1 cut(s) 132
BseJI GATNNNNATC 1 cut(s) 369
BseMI GCAATG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 2 cut(s) 5, 281
BshNI GGYRCC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 5, 281
Bsp1407I TGTACA 1 cut(s) 51
BspACI CCGC 1 cut(s) 98
BspANI GGCC 2 cut(s) 5, 281
BspLI GGNNCC 2 cut(s) 30, 282
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 22, 307
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 26, 35, 44
BstV1I GCAGC 1 cut(s) 29
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 2 cut(s) 5, 281
BtgI CCRYGG 1 cut(s) 284
BtsCI GGATG 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 22, 307
CaiI CAGNNNCTG 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 251, 280
Csp6I GTAC 2 cut(s) 52, 156
CviJI RGCY 5 cut(s) 5, 20, 101, 231, 281
CviKI_1 RGCY 5 cut(s) 5, 20, 101, 231, 281
CviQI GTAC 2 cut(s) 52, 156
DriI GACNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 88
FaiI YATR 7 cut(s) 36, 61, 63, 88, 104, 174, 326
Fnu4HI GCNGC 2 cut(s) 18, 99
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 2 cut(s) 18, 99
GluI GCNGC 2 cut(s) 18, 99
HaeIII GGCC 2 cut(s) 5, 281
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 263, 289
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 2 cut(s) 86, 197
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 35, 44
HpySE526I ACGT 1 cut(s) 42
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 5 cut(s) 6, 138, 225, 234, 267
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 73, 355
MaeII ACGT 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 183, 219, 244
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 2 cut(s) 280, 297
MnlI CCTC 5 cut(s) 61, 64, 146, 152, 271
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 88
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 20, 231
Mva1269I GAATGC 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 26, 35, 44
NlaIV GGNNCC 2 cut(s) 30, 282
NsiI ATGCAT 1 cut(s) 88
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 2 cut(s) 19, 100
PleI GAGTC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 43
PspCI CACGTG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 30, 282
PspPI GGNCC 2 cut(s) 251, 280
PstNI CAGNNNCTG 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 20, 231
RsaI GTAC 2 cut(s) 53, 157
RsaNI GTAC 2 cut(s) 52, 156
SatI GCNGC 2 cut(s) 18, 99
Sau96I GGNCC 2 cut(s) 251, 280
SchI GAGTC 1 cut(s) 272
SetI ASST 6 cut(s) 22, 34, 45, 60, 196, 233
SfaNI GCATC 2 cut(s) 73, 355
SinI GGWCC 1 cut(s) 251
Sse9I AATT 3 cut(s) 183, 219, 244
SsiI CCGC 1 cut(s) 98
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 45
TasI AATT 3 cut(s) 183, 219, 244
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 1 cut(s) 289
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 2 cut(s) 150, 192
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 2 cut(s) 219, 244
Zsp2I ATGCAT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.